Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,173	T→C	L155P (CTC→CCC)	hyi →	hydroxypyruvate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,173	0	T	C	100.0%	208.5 / NA	65	L155P (CTC→CCC)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/34);  total (31/34)

GCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTG  >  NC_000913/535929‑536416                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    gCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCg                                                                                                                                                                                                                                                <  1:120324/250‑1 (MQ=255)       aTTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTc                                                                                                                                                                                                                                          <  1:58193/250‑1 (MQ=255)          ccTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGc                                                                                                                                                                                                                                       <  1:121324/250‑1 (MQ=255)             ggCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAgg                                                                                                                                                                                                                                    <  1:182430/250‑1 (MQ=255)             ggCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGGCTGGTCGGTAAAACGCCGGCTGGTTTCAGAAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGAGTTATGCCACGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTTTTAACCATTTTGATATTCCTGGTTTCAATCCCACCGGCACTcggccggc                                                                                                                                                                                                                                   >  2:360971/1‑251 (MQ=255)                      gaagaTTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGGTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAatt                                                                                                                                                                                                                          <  2:574023/251‑1 (MQ=255)                       aagaGTTTCGGGATGGCGTAGCAGCAGCGATTCGGTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCGGGTTTCCGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATAGTCCTGGTTTCCATCCCACCGGAACGCGTCAGGCGCTGAAattg                                                                                                                                                                                                                         <  2:333997/251‑1 (MQ=255)                          agTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAattgatt                                                                                                                                                                                                                      <  2:197661/251‑1 (MQ=255)                                               cagcGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGAAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAt                                                                                                                                                                                                 <  1:305653/251‑1 (MQ=255)                                                   gATTCGTTATGCCCGTGCTCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGAGATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGa                                                                                                                                                                                             <  2:554317/251‑1 (MQ=255)                                                      tCGTTATGACCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGATTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATAAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGaaa                                                                                                                                                                                           >  1:342775/1‑250 (MQ=255)                                                           aTGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTc                                                                                                                                                                                       >  1:133905/1‑249 (MQ=255)                                                               ccGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATg                                                                                                                                                                                 <  1:437191/251‑1 (MQ=255)                                                                     gcTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACAttt                                                                                                                                                                           <  1:114536/251‑1 (MQ=255)                                                                         ggTAAAAAAAAAATTAACTGTCTGTACGGTAATACGCCGGCTGGTTTAAGCAATGAACAGATTCACGCAACGCTAGTCGAAAAAATGCGTTATGCAACGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAAGTGATTAACAATTTTGATATTCATGGTTTCCATCCCACCGGAACTAGGAATGCGCTAGAATTGATTGATGATGTTGGGTTCGGCAATTTGACAATTCAGTATGACATTTagct                                                                                                                                                                       >  2:432768/1‑248 (MQ=255)                                                                            aataaAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCatat                                                                                                                                                                    <  1:120444/251‑1 (MQ=255)                                                                             ataaAAAAATTAACTGTCTGGTAGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGCATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGCACTCGGCAGGCGCTGAACTTGATTGGTGATGTTGGTTGCTACAATTTGAACATTCAGTATGACATTTATCATATg                                                                                                                                                                   >  2:304081/1‑251 (MQ=255)                                                                              taaAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGTTGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGc                                                                                                                                                                  >  2:316043/1‑251 (MQ=255)                                                                                         aCTGTCTGGTCGTTAAAATGCCCGCTGGTTTCAGCAGTGAACAGTTCCACGCAACGCTGGTATAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACGGATTGAACCTATTATCCATTTTGATACTCCAGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGCAATTGATTGATGATGTTGGTTGCGGCAATTTGAAAAGTCATTATGACATTTATCATTTGCAGCGGACGGaa                                                                                                                                                       <  2:497653/251‑1 (MQ=255)                                                                                           tGTCTGGTGGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAAACTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAATATTCAGTATGACATTTATCATATGCAGCGGATGGAAgg                                                                                                                                                     >  2:202729/1‑251 (MQ=255)                                                                                            gTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGt                                                                                                                                                    <  1:56982/251‑1 (MQ=255)                                                                                               tGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGa                                                                                                                                                  >  1:90707/1‑250 (MQ=255)                                                                                                  tCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAAtt                                                                                                                                               >  1:16992/1‑250 (MQ=255)                                                                                                        aaaCGCCGGCTGGTTTCAGCAGTGAACAGATTCACGAAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAac                                                                                                                                        >  2:97826/1‑251 (MQ=255)                                                                                                          aCGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGAGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAacac                                                                                                                                      >  1:164629/1‑251 (MQ=255)                                                                                                              cGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATg                                                                                                                                  <  1:124815/251‑1 (MQ=255)                                                                                                                gCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGAc                                                                                                                                <  1:514077/251‑1 (MQ=255)                                                                                                                   ggTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGAGAATATGCTGATGAACGAAGATATTTTCTTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGGGAATTAACCAACACAATGACTc                                                                                                                              >  2:27179/1‑250 (MQ=255)                                                                                                                                       tCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGt                                                                                                          <  1:292429/250‑1 (MQ=255)                                                                                                                                        cACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCTAATATGCTGATTAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGta                                                                                                         <  2:407239/250‑2 (MQ=255)                                                                                                                                        cACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTc                                                                                                         <  1:233278/250‑1 (MQ=255)                                                                                                                                         aCGCAACGCTTGCAGAAAACCTGCGTAATGCCGCGCCTATGGGGATGAAAGAAGATATTTTATTACTGATTGAAACTATTAACCATTTTGATATTCCAGGTTTCCATCCCACCGGAACTCGGCAGGCCCTGAAATTGATTGATGTTGTTGGTTGTTGCACTTTGAAAATTCAGTAAGGCATTTATCATTTGCAGCGGGTGGAAGGGGAATTACCCAACACAAAGACTCAGTGTGCTGATAAAATTGGtaac                                                                                                       >  2:387618/1‑248 (MQ=255)                                                                                                                                             aaCGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATGTTATTACTGATTGAACCTATGAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTTCAATTTGAAAATTCAGTATGACATTGATCATATGCAGCGGATGGAAGGTGAATTAACCAACTCCATGACTCAGTGGGCTGATAAAATGGGTCCCCTGc                                                                                                   <  2:38282/251‑1 (MQ=255)                                                                                                                                                gCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCaa                                                                                                 >  1:460801/1‑250 (MQ=255)                                                                                                                                                   tGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTg                                                                                             >  1:192860/1‑251 (MQ=255)                                                                                                                                                         caaCCTGCGATATGCCGCGAATATGCTGATGCAAGAAGATATTTTAGTACTGATTGAACCTATTAACCATTTTAATATTCCTGGTTTCCATCCCACCGGAACTCGGAAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAGTTTGAACATTCAGTCTGACATTTATCATATGCAGCGGATGGAGGGTAAATTAACCAAAACCATGCCTCAGTGGGCTGATAAAATTGGTCACATGCAAATTGCCGAt                                                                                        >  2:88386/2‑250 (MQ=255)                                                                                                                                                            ccTGCGTTATGCTGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGACCCTATTAACCATTTTGATATTCATGGTTTCCATCCCACTGGAACTCGGCAGGCGCTTAAATTGATTGCTGATGATGGGTGCGCCACTTTGACAATTCAGTATGACATTTATCATATGCAGAGGATGGAAGGTGAATTCAACAACACCATAACTCAGTGGGCTGATAAAATTGGTCACCTGCAAAttccagagactc                                                                                    >  2:299477/1‑241 (MQ=255)                                                                                                                                                                 gTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCAt                                                                               <  2:244144/251‑1 (MQ=255)                                                                                                                                                                   tATGCCTCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATc                                                                              <  1:283376/250‑1 (MQ=255)                                                                                                                                                                           gAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaac                                                                     >  1:43326/1‑251 (MQ=255)                                                                                                                                                                             atatGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccg                                                                   <  1:175889/251‑1 (MQ=255)                                                                                                                                                                                       gaAAGAAGATATTTTATTACTGCTTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGATGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg                                                           >  1:428818/1‑249 (MQ=255)                                                                                                                                                                                        aaaGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATCTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCCTCGCGGCgaaccgggaaccggcg                                                         >  2:570350/1‑248 (MQ=255)                                                                                                                                                                                          agaagaTATTTTATGACTGATTGAACCTATTAACCATTTTGATATTCCTGGTGTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCATTATGACATTGATCATATGCAGCGGATGGAAGGTGAATTACCCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGaaa                                                      <  2:347644/251‑1 (MQ=255)                                                                                                                                                                                           gaagaTATTTTATTACTGATTGAACCTAGTAACCATGTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATTTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGTCGAACCGGGAACCGGAGaaaa                                                     <  2:127188/251‑2 (MQ=255)                                                                                                                                                                                               atatTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAaattaat                                                 <  1:304081/251‑1 (MQ=255)                                                                                                                                                                                                         aCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTAtc                                       <  1:308716/251‑1 (MQ=255)                                                                                                                                                                                                            gATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCCCCGTAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGGTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATGAATTATGATTAtct                                      <  2:349466/249‑1 (MQ=255)                                                                                                                                                                                                            gATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTAtct                                      <  1:571099/249‑1 (MQ=255)                                                                                                                                                                                                            gATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCAGCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTCATTATGATTATctct                                    <  2:437633/251‑1 (MQ=255)                                                                                                                                                                                                             aTTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCtt                                   >  1:392408/1‑251 (MQ=255)                                                                                                                                                                                                                 aaCCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAg                               <  1:1216/251‑1 (MQ=255)                                                                                                                                                                                                                 aaCCTATTAACCATTTTGATATTCCTGGATTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTa                                 >  1:218827/1‑249 (MQ=255)                                                                                                                                                                                                                      aTTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACGCCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAAt                          <  1:27179/251‑1 (MQ=255)                                                                                                                                                                                                                        tAACCATTTTGATATTCCTGGTTTCCATCCCACCTGAACTCGGCAGGCGCTGCAATTGATTGATGATGTTGGTTGCCTCCATTTGACAATTCAGAATGACATTAATCATATGAAGCGGGTGGCAGGTGAATTAACCAACACCATGACACAATGGGCTGATAAAATTGGTCAACTGAAAATTGAAGATAATCCGCATCGAGGCGAACCGGGAACCGGAGAAATTCATTCTGATTATCTCTTTCAGGTGATCg                        >  2:156007/1‑251 (MQ=255)                                                                                                                                                                                                                         aaCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCg                        >  2:445425/1‑250 (MQ=255)                                                                                                                                                                                                                          aCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGTAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGGAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGaa                      <  2:189731/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCATTTTGATATTCCTGGTTTCCATACCACCGGAACTCGGCAGGCTCTGAAATTGATTGATGATGTTGGGTGCTGCAATTTGAAAATTCAGTATGAAATTTATCATATGCAGAGGATGGAAGGTGAAATAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAAACGGGAACCGGAGAAATTAATTATTAATATCCCTTTAAGGAAATCGa                       >  2:363574/1‑250 (MQ=255)                                                                                                                                                                                                                             aTTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCGGCAATTTGATAATTCAGTATGACATATATCATATGAAGCGGATGGATGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCAACTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGTGAAATTAATTATGATTAACTCTATAGGGTAATCGAAAAt                   >  2:156065/1‑251 (MQ=255)                                                                                                                                                                                                                               tttGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTc                 >  1:550988/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGCTGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGATCCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTg               >  1:392622/1‑251 (MQ=255)                                                                                                                                                                                                                                  gATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTg               >  1:157469/1‑250 (MQ=255)                                                                                                                                                                                                                                           ggTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACgg     >  1:36394/1‑251 (MQ=255)                                                                                                                                                                                                                                              ttCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACggtt   <  2:90707/250‑1 (MQ=255)                                                                                                                                                                                                                                              ttCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGATTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACggttg  >  1:317338/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTG  >  NC_000913/535929‑536416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑