Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,289	A→G	Y235H (TAT→CAT)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,289	0	A	G	100.0%	185.3 / NA	53	Y235H (TAT→CAT)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/22);  total (31/22)

ATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGC  >  NC_000913/4312044‑4312532                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    agttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGc                                                                                                                                                                                                                                               <  1:125738‑M2/238‑1 (MQ=255)    tttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGcc                                                                                                                                                                                                                                              >  2:442872‑M2/11‑249 (MQ=255)      tgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGtt                                                                                                                                                                                                                                          >  1:20637‑M2/9‑251 (MQ=255)        acgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCGTGGTGATAATAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCTTCCAGCAAGGTTGGTTGTAATATTCTTGGCGTTGCATTGCGTCCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTc                                                                                                                                                                                                                                        <  1:32064‑M2/245‑1 (MQ=255)          gtgaggTAAGGGGACAG‑TAACTCGGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGGGCGAAGAAATCAACAATTGCGACGGTTTTTTCCGTATTAATATTAGATTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCAAGCAAGGTTGTTTGTAATATTCCTGCCGAGGCAATACGACCTAATAACTGCGTTTGCGGGGCTGGTGAGCAAATAAAATGATCGGGCCGTTTCGc                                                                                                                                                                                                                                      >  2:571413/5‑251 (MQ=255)                       gacagtacctcagcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGAGAGAAGAAATCAACACTTGCGCAGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGATGGGCGCCAGAGACAAAAAAAGCGTACAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGTCTGGGGAGCAAATAATATGACAGGCCAGCTTCTCTATCGGTGagtg                                                                                                                                                                                                                          >  2:305131/13‑248 (MQ=255)                        acagTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATgg                                                                                                                                                                                                                         >  1:470519/4‑251 (MQ=255)                        acagTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATgg                                                                                                                                                                                                                         >  1:355594/4‑251 (MQ=255)                          agTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGt                                                                                                                                                                                                                        >  1:493651/2‑250 (MQ=255)                          agTAACGCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGACTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTTGGCGACAGAGGCAAAAAAAGCGGCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGGTTCCGGGGCTGGTGAGCAAATAATATGATCGTCCAGTTTCGCTATCGGTGAAGGGTa                                                                                                                                                                                                                       <  2:166123/250‑1 (MQ=255)                                          cGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACACTTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGACTGGTAGCTATGGGTTATACa                                                                                                                                                                                                        >  2:355488/1‑250 (MQ=255)                                             aaTTACCTTTTTCTTATTTCAGCGCGACTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGGTGGTTGGAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAaat                                                                                                                                                                                                     >  1:127829/1‑250 (MQ=255)                                                        tCTTATTTCAGCGCGCCTGGTGAGAAGAAAACAACAATTGCGCCGGTTTTTTCCCTCTTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGGTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCCAATAATATGATCGGGCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGcccc                                                                                                                                                                                         >  1:547695/1‑251 (MQ=255)                                                              ttCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAA‑GCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCtt                                                                                                                                                                                   <  1:234586/250‑1 (MQ=255)                                                               tCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCttt                                                                                                                                                                                  >  1:65257/1‑251 (MQ=255)                                                                cAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGCGGCATTACGACATAATAACGGCGATTCCGGGGCTGGCGAGCAAATAATATGATCGGCCAGTTTCGCCATCGGTGAATGGTAGATATGGGTTATACAAATAACATTTGCCCCGTTCtttt                                                                                                                                                                                 >  2:1565/1‑251 (MQ=255)                                                                   cgcgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTCTACAAATAATCTTTGCCCCGTTCTTTTTTg                                                                                                                                                                              >  1:542304/1‑251 (MQ=255)                                                                              agaagaAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACg                                                                                                                                                                   >  1:491661/1‑251 (MQ=255)                                                                                aagaaATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGc                                                                                                                                                                 >  1:333443/1‑251 (MQ=255)                                                                                    aaTCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCt                                                                                                                                                             >  1:231216/1‑251 (MQ=255)                                                                                       caacaaTTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCttt                                                                                                                                                           <  1:129390/250‑1 (MQ=255)                                                                                            aTTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGGTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTGTACGGCCGCTTTTACATc                                                                                                                                                     <  2:493651/251‑1 (MQ=255)                                                                                               gcgcCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGGGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACTGCCGCTTTTACATccct                                                                                                                                                  >  2:197090/1‑248 (MQ=255)                                                                                                                      tttaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGAATTACGACCTAATAACGGCGTTTCAGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGACTGGGAGCTATGGGTTATACAAATAAACTTTGCCCCGTTCTTTTTTGCCAATTCTACGGCCGCTTTTACATCAATGGTCCGCCCGGAATGGGTCACt                                                                                                                            >  2:127920/2‑250 (MQ=255)                                                                                                                      attaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGAATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACt                                                                                                                            >  2:68817/1‑250 (MQ=255)                                                                                                                        taGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCTGAATGGGTCACTAc                                                                                                                          <  2:217725/250‑1 (MQ=255)                                                                                                                           cTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGc                                                                                                                      >  1:580051/1‑251 (MQ=255)                                                                                                                                taaaTGTTGAGCTGTGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATACTTGCCGAGGCATTACGACCTAATAACGTCGTTTCCTGGGCTGTTGAGCAAATAATATGATCGGCCAGTTTCGCTATCTGTGAATGGTAGCTATGGGTTATACAAAGAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTGTTACATCACTGGCTCGCCCGGAATAGGTCACGACCAGCacaa                                                                                                                  <  2:518795/248‑1 (MQ=255)                                                                                                                                 cAATGTTGAGCTGTGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCTCCCGTAATGGGTCACTACCAGCacaac                                                                                                                 <  1:129/250‑1 (MQ=255)                                                                                                                                        gAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcct                                                                                                          <  1:569049/250‑1 (MQ=255)                                                                                                                                             gggCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttcct                                                                                                      <  1:342226/249‑1 (MQ=255)                                                                                                                                                  aCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTaaca                                                                                                >  1:341370/1‑250 (MQ=255)                                                                                                                                                      agaCAAAAAAAGCGTCCATCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGAGTTTCCGGGGCTGGTGAGCAAATAATATGATCGACCAGTTTCGCTATCGGAGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGa                                                                                            >  2:495174/1‑250 (MQ=255)                                                                                                                                                              aaaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGAACTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcat                                                                                   <  2:388653/251‑1 (MQ=255)                                                                                                                                                               aggCGTCCAGCAAGGTTGGTTGTAATAGTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCGGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCCACAGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatc                                                                                  <  2:90389/249‑1 (MQ=255)                                                                                                                                                               aaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGACACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatc                                                                                  >  1:289934/1‑251 (MQ=255)                                                                                                                                                                  cGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCCGCACAACAtct                                                                                                             >  2:15440/1‑221 (MQ=255)                                                                                                                                                                  cGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtct                                                                                                             <  1:15440/221‑1 (MQ=255)                                                                                                                                                                    tCCAGCAAGGTTGGTTGTCATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACACATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatca                                                                              >  2:130560/1‑250 (MQ=255)                                                                                                                                                                    tCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat                                                                             >  2:73763/1‑251 (MQ=255)                                                                                                                                                                                           tCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATACTATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGCTGTGAGCATCAGGATAGGCCt                                                      >  1:328723/1‑251 (MQ=255)                                                                                                                                                                                           tCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGcc                                                       <  1:495174/250‑1 (MQ=255)                                                                                                                                                                                                  gAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCg                                               <  1:442872/251‑1 (MQ=255)                                                                                                                                                                                                     gCATTACGACCTAATAACGGCGTTGCCGGGGTTGGTGAGCAAATAATATGATCGGCCAGTTGCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTGTTTTGCCATTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCTGGATAGGCCTGACAGCGTAc                                            <  1:232677/251‑1 (MQ=255)                                                                                                                                                                                                             cccTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCCCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATgg                                    <  2:470519/250‑2 (MQ=255)                                                                                                                                                                                                                 aataaCGGCGTTTCCGGGGCTGGTGTGCAAATCATATGATCGGCCAGTTTCGCTAGCGGTGAATGGTAGCTATGTGTTATACAAATAATCTTTGCCCCGTTCTTTTGTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGTAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATgggc                                  <  2:309764/249‑4 (MQ=255)                                                                                                                                                                                                                 aataaCGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATgggc                                  <  1:354263/249‑4 (MQ=255)                                                                                                                                                                                                                     aCGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCCGGATAGGCCTGACAGCGTCCGCCAATGGGAAAGAAc                            >  2:563102/1‑251 (MQ=255)                                                                                                                                                                                                                        gCGTTTCCGGGTCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATTGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGACTGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAACGGACATCATCATGATGTGCGCATCAGGATCGGCCTGACAACGTACGCCAATGGGCAAGAACTtg                         >  2:393659/1‑251 (MQ=255)                                                                                                                                                                                                                              ccGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCa                                                                >  1:282734/1‑206 (MQ=255)                                                                                                                                                                                                                              ccGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCa                                                                <  2:282734/206‑1 (MQ=255)                                                                                                                                                                                                                                ggggCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCGTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCGGTAc                 <  2:304933/251‑1 (MQ=255)                                                                                                                                                                                                                                         gAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGcac         >  1:117544/1‑250 (MQ=255)                                                                                                                                                                                                                                               ataatatgatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCCCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACCTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCCTCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGAGCTGTACATCAGCACAGATAGc  >  2:140700/1‑251 (MQ=255)                                                                                                                                                                                                                                               ataatatgatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGc  <  1:563102/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGC  >  NC_000913/4312044‑4312532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑