Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,235,651	T→C	intergenic (+244/‑255)	pgi → / → yjbE	glucose‑6‑phosphate isomerase/uncharacterized protein YjbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,235,651	0	T	C	100.0%	160.1 / NA	46	intergenic (+244/‑255)	pgi/yjbE	glucose‑6‑phosphate isomerase/uncharacterized protein YjbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

TAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTC  >  NC_000913/4235405‑4235888                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              tAATCATCGTCGTTATTTAGGTCGTATAAGCCGTTCACGCCGCATCCGGCAACCGATGCCTTATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTTCATCGTATTCGGCAATTAATACATAGCACGATGGATTAAATAACCTTAATAACAATGCAGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCt                                                                                                                                                                                                                                            <  2:556644/250‑1 (MQ=255) tAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCCCGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGGGGTTAACCCt                                                                                                                                                                                                                                            >  2:340083/1‑250 (MQ=255)     catcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGAGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAAt                                                                                                                                                                                                                                       >  1:8718/1‑251 (MQ=255)         gtcgATATGTAGGCCGGATACGGCGTTCACGCCGCATCCTGCAACCGATGCCTGATGCGACGCGGTCGTGTCTTAGCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGTCGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTATCCCTAAAATAGAt                                                                                                                                                                                                                                   <  2:440243/251‑1 (MQ=255)               aggtgagcccgATAAGGAGTTCGTGCCGCATCCTGCAAGCAATGCCGGATGCGACTCGTTCGCGTCTTATCAGGTCTTCAGGTCGATGCCGATATGTACATCGTATTCGGTAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGACGGCATTAGTTTATCAGATAAATCCCCTAGTCTGTAATTTAACGGATATCATACGGTGAGGTTAACCCTAAAATAGATTTGTaa                                                                                                                                                                                                                             <  2:185189/241‑1 (MQ=255)                    ggCCGGATAAGGAGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCgtt                                                                                                                                                                                                                        >  1:130698/1‑251 (MQ=255)                                          gcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAg                                                                                                                                                                                                  <  1:371095/251‑1 (MQ=255)                                                        aTGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTg                                                                                                                                                                                    <  2:117280/251‑1 (MQ=255)                                                              gATGCGACGCGGTCGCGTCTTAGCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGtttt                                                                                                                                                                                <  2:427647/249‑1 (MQ=255)                                                                         ggcgcgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTGTATTTTAAGTTTa                                                                                                                                                                   <  2:565313/249‑1 (MQ=255)                                                                             ggTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGAt                                                                                                                                                               <  2:189024/250‑1 (MQ=255)                                                                               tCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGACAATACGAAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGAttt                                                                                                                                                             >  2:27772/1‑251 (MQ=255)                                                                                   aTCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCTGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTAt                                                                                                                                                         <  2:525200/251‑1 (MQ=255)                                                                                      aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGt                                                                                                                                                      <  1:347366/251‑1 (MQ=255)                                                                                            aCAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTAttt                                                                                                                                                 <  1:367588/250‑1 (MQ=255)                                                                                             cAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTa                                                                                                                                                >  1:389167/1‑250 (MQ=255)                                                                                                  cGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAatat                                                                                                                                           >  2:323366/1‑250 (MQ=255)                                                                                                        cGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGACTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTg                                                                                                                                     >  2:490487/1‑250 (MQ=255)                                                                                                          atatGTACATCGTCTTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGTCGTTATGTCGGCATTTTTTTATCAGATAAATCACCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAACATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTCATTGCTTTTGTTTTTATTTTAAGTTTATNATTTTTATTGTTATTTAAATATAAGTTGaa                                                                                                                                   >  2:290055/1‑250 (MQ=255)                                                                                                                       aTTCGGCAATTAATGCATATCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGGCGGCATTTTTGTATCAGATAAATCCCCTTATCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAACTAGATTTTTATTCGTTTTTTATTTCGGAAAATACGCAGATTAATTGCAATTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATAttttat                                                                                                                      <  2:106305/250‑4 (MQ=255)                                                                                                                       aTTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGc                                                                                                                                                                                                    <  2:185261/172‑1 (MQ=255)                                                                                                                       aTTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCACTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGc                                                                                                                                                                                                    >  1:185261/1‑172 (MQ=255)                                                                                                                           ggCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATattc                                                                                                                  >  1:526472/1‑250 (MQ=255)                                                                                                                           ggCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATattc                                                                                                                  >  1:509215/1‑250 (MQ=255)                                                                                                                                                    ttAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGAttttt                                                                                                                                                           >  1:337472/1‑184 (MQ=255)                                                                                                                                                    ttAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTGTAAGTTTATGAttttt                                                                                                                                                           <  2:337472/184‑1 (MQ=255)                                                                                                                                                         tAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAAt                                                                                   >  2:59198/1‑251 (MQ=255)                                                                                                                                                          aaCCTTAAGAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAAt                                                                                   <  2:231717/250‑1 (MQ=255)                                                                                                                                                              ttAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTcccc                                                                              >  1:283787/1‑251 (MQ=255)                                                                                                                                                                aataaCAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCcgc                                                                            <  1:519573/251‑1 (MQ=255)                                                                                                                                                                              cGTTATGTCGGCATTTTTTTATCAGATAAATCGCATTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGCAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGcct                                                              >  2:222294/1‑249 (MQ=255)                                                                                                                                                                               gTTATGTCGGCATTTTTTTAGCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATg                                                             <  2:101812/251‑1 (MQ=255)                                                                                                                                                                                  aTGTCGGCATTTTGTTATCAGAGAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCTGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATGGTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGcg                                                           <  2:319846/250‑2 (MQ=255)                                                                                                                                                                                     tCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTACAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCttt                                                         >  1:152949/1‑249 (MQ=255)                                                                                                                                                                                              ttttATCAGATAATTCACCTTGTCTGTAATCTAACGGAACTCCTACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAGACTTATATTTGATATTCATTCCATTTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTACTTCGCATGCTTTAGTTGGGtat                                               >  2:228474/1‑250 (MQ=255)                                                                                                                                                                                              ttttATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTAATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGtat                                               >  1:34906/1‑250 (MQ=255)                                                                                                                                                                                                  aTCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTc                                           <  2:389167/250‑1 (MQ=255)                                                                                                                                                                                                             ccccTTGTCGGTAATTTAACGGAAATCAGANCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCAGCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATGCTCGATcccgcccg                                <  2:268026/250‑1 (MQ=255)                                                                                                                                                                                                                cTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAAt                            >  1:387703/1‑251 (MQ=255)                                                                                                                                                                                                                 ttGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATg                           >  1:46634/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTAAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTggga                  <  2:124510/251‑2 (MQ=255)                                                                                                                                                                                                                                   aaaTCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCTTTCCAATTATCCTAAAACGCCATCCATAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCTTTCCCGCCCGAATTGTTTTGGGATACATCTccc         >  2:385967/1‑250 (MQ=255)                                                                                                                                                                                                                                        aTACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCcattcat    <  1:108066/251‑1 (MQ=255)                                                                                                                                                                                                                                          accttGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCattcattc  <  2:208238/247‑1 (MQ=255)                                                                                                                                                                                                                                               gAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAAc                                                                                               <  2:101705/153‑1 (MQ=255)                                                                                                                                                                                                                                               gAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAAc                                                                                               >  1:101705/1‑153 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              TAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTC  >  NC_000913/4235405‑4235888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑