Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,512,183	T→C	E54E (GAA→GAG)	yhfZ ←	putative DNA‑binding transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,512,183	0	T	C	100.0%	194.9 / NA	53	E54E (GAA→GAG)	yhfZ	putative DNA‑binding transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

CCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTGCACATACCACGTTGTTGATATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCG  >  NC_000913/3511944‑3512423                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 aattctggccttcttaatgactctttttatctattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGtttt                                                                                                                                                                                                                                       >  2:233216‑M2/183‑251 (MQ=255)         ccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatcttatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCACCGCgg                                                                                                                                                                                                                               >  2:331378‑M2/175‑251 (MQ=255)                   gactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCa                                                                                                                                                                                                                      <  1:551009‑M2/86‑1 (MQ=255)                                 attcagcaaataaaacaaggccccttaataaatattatctgctaattggttagattctccgttccagcgacttgaaaaattaagtcattcgctcactgacgccaattaaatattcctgtagcgttcttaacgggcagtcgtcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGGTCAATCCGTATCGCTCCGCGTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAAc                                                                                                                                                                                                       <  2:243967‑M2/101‑1 (MQ=255)                                                   agccccttaataaatattatctgctaatgtgttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCACCGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCt                                                                                                                                                                                     <  2:425259‑M2/119‑1 (MQ=255)                                                            ataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATgg                                                                                                                                                                            >  2:169155‑M2/124‑251 (MQ=255)                                                                  attatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGAGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTc                                                                                                                                                                       >  2:139658‑M2/118‑250 (MQ=255)                                                                         gctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGAtt                                                                                                                                                               >  1:477143‑M2/111‑251 (MQ=255)                                                                          ctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTCTCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCACCGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCTattc                                                                                                                                                              >  2:552364‑M2/110‑250 (MQ=255)                                                                            aatggtttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTAc                                                                                                                                                             <  1:225013‑M2/143‑1 (MQ=255)                                                                               gggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCa                                                                                                                                                         <  1:449261‑M2/147‑1 (MQ=255)                                                                                ggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTAAATCGATTACTGCa                                                                                                                                                         >  2:2293‑M2/104‑250 (MQ=255)                                                                                          accgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCAccc                                                                                                                                               >  2:199765‑M2/94‑250 (MQ=255)                                                                                                acagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGCCTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCACCAAAt                                                                                                                                        >  2:25652‑M2/88‑251 (MQ=255)                                                                                                    cgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCTCTTTTCACCCAACAAATAACg                                                                                                                                    <  1:102098‑M2/168‑1 (MQ=255)                                                                                                    cgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACg                                                                                                                                    >  2:238987‑M2/84‑251 (MQ=255)                                                                                                                  aagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCCCCCAACAAATAACGGGCCa                                                                                                                               >  2:371708‑M2/70‑242 (MQ=255)                                                                                                                  aagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCa                                                                                                                               <  1:371708‑M2/173‑1 (MQ=255)                                                                                                                    gtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAAAGGGCCAGCGTCGtgatg                                                                                                                    >  2:555058‑M2/68‑251 (MQ=255)                                                                                                                          cgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGacgacg                                                                                                              >  1:138110‑M2/62‑251 (MQ=255)                                                                                                                              cactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCct                                                                                                           <  2:138110‑M2/193‑1 (MQ=255)                                                                                                                                 tgacgccaattaaatattactgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAAATGCCATTGCGCACACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGACAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACACAACAAATAACGGTCCAGCGTCATGAGGACGACGActtctt                                                                                                       >  2:492099‑M2/55‑251 (MQ=255)                                                                                                                                  gacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCttt                                                                                                      <  1:435442‑M2/198‑1 (MQ=255)                                                                                                                                  gacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGACAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCttt                                                                                                      >  1:346387‑M2/54‑251 (MQ=255)                                                                                                                                      ccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACAAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAACGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTg                                                                                                  >  2:402310‑M2/50‑251 (MQ=255)                                                                                                                                            aaatattcctgtatcgttcttaacgagcagtatatgtatatgcCTCTACCAGATAACTGCCATTGCGTCCACGGCGTTCAATCCGGATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTGTTCAAGCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGTCTTCTTTTTTGATAAAc                                                                                            <  2:367163‑M2/208‑1 (MQ=255)                                                                                                                                                attcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCGTTTTTGATAAACGtt                                                                                         <  1:139658‑M2/211‑1 (MQ=255)                                                                                                                                                 ttcctgtatcgttcttaacgagcagtcttcgtatattcCTCTACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGAGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCTCACTTTTCACCCAACAAATAACGGACCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCg                                                                                       >  1:6518‑M2/39‑251 (MQ=255)                                                                                                                                                  tcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCg                                                                                       <  2:304407‑M2/213‑1 (MQ=255)                                                                                                                                                   cctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGAc                                                                                     <  1:438171‑M2/215‑1 (MQ=255)                                                                                                                                                   cctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACAGACATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCATCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGAc                                                                                     >  1:576452‑M2/37‑251 (MQ=255)                                                                                                                                                   cctgtatcgttcttaacgagcagtcttcgtatattcCCCGACCAGATAACTGCCATTGCGCCCACGGCGGTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGAc                                                                                     <  1:102707‑M2/215‑1 (MQ=255)                                                                                                                                                        atcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACTGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCata                                                                                <  1:494835‑M2/220‑1 (MQ=255)                                                                                                                                                        atcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgac                                                                                                                  >  2:439503‑M2/32‑217 (MQ=255)                                                                                                                                                        atcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgac                                                                                                                  <  1:439503‑M2/186‑1 (MQ=255)                                                                                                                                                          cgttcttaccgagccgtcttcgtatattcCTCGACCAGATAACCGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCTCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCataa                                                                               <  2:124706‑M2/221‑1 (MQ=255)                                                                                                                                                          cgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCCCGGCGTTCAATCCGTAGCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCataat                                                                              <  2:419986‑M2/222‑1 (MQ=255)                                                                                                                                                               ttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTCATAAACGTTCGCAGCATAATAAAtt                                                                         >  2:540152‑M2/25‑251 (MQ=255)                                                                                                                                                                    gagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTttccg                                                                    >  1:529341‑M2/20‑249 (MQ=255)                                                                                                                                                                     agcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGt                                                                   <  1:559017‑M2/233‑1 (MQ=255)                                                                                                                                                                      gcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATACTAAATTTTCAGt                                                                   >  2:424195‑M2/18‑250 (MQ=255)                                                                                                                                                                       ccgtcttcgtatattcCACGACCCGATAACCGCCATCGCGCCCTCGGCGTTCAAGCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACTGATGAACGGCATTCATTTTCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCCCCCAACAAATAACGGGCCAGCGTCGAGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTaa                                                                 <  2:576452‑M2/235‑1 (MQ=255)                                                                                                                                                                         gtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATc                                                                                                                                                                   >  2:415621‑M2/15‑151 (MQ=255)                                                                                                                                                                         gtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATc                                                                                                                                                                   <  1:415621‑M2/137‑1 (MQ=255)                                                                                                                                                                          acttcgtatatccctcGACCAGTTAACTGCCACTGCCCAAACGGCGTACAACCCGTATCGATACGCTTGTCTCCAATATGTTCAACGCTGCCTTCGTTAGTCTAAGGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCTCTTTCCGCCCAACACATATCGGGCAATCGTCGTGATGACGAAGCCTTCTTTTTTGATAATCGTTCGACGCCTAATTAATATTCAGTAAAtg                                                              <  2:80514/238‑2 (MQ=255)                                                                                                                                                                                 atattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAtt                                                       <  1:444185‑M2/245‑1 (MQ=255)                                                                                                                                                                                 atattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTGTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGGCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAatat                                                        <  1:342029‑M2/244‑1 (MQ=255)                                                                                                                                                                                  tattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAttt                                                      >  1:336898‑M2/6‑251 (MQ=255)                                                                                                                                                                                         cGACCAGATAACTTCCATGGCGCCCACGGCGGTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACTACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCtt                                               <  2:335594/251‑1 (MQ=255)                                                                                                                                                                                                       ccATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGa                                    >  1:58318/1‑248 (MQ=255)                                                                                                                                                                                                                                     gCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGc   <  1:192202/251‑1 (MQ=255)                                                                                                                                                                                                                                      cTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATCTTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCg  >  1:541756/1‑251 (MQ=255)                                                                                                                                                                                                                                        ccGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCg  <  2:477143/249‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 CCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTGCACATACCACGTTGTTGATATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCG  >  NC_000913/3511944‑3512423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑