| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,396 | 0 | C | T | 20.3% | 121.8 / 11.3 | 59 | S39F (TCT→TTT) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base C (13/34); new base T (12/0); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCC > NC_000913/119156‑119639 | gattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGgcg < 1:562599/250‑1 (MQ=255) gCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACg < 1:556083/250‑1 (MQ=255) gATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGTTCCTGCATTTGATTGGGCCCTGTTTCGTGTGCTGGTCAGCAAGGAGACAACACGACGCTATTTACAACCTTACTGGTGTTCATTTTCGATCCCCTTTTTAAGTTGGGCGTGCACTGGCAGCTTCAGCATCGTCTCCAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑g < 1:252369/251‑1 (MQ=255) cTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGTAAAATTTTTTTCTCCGgcgccagttgggcaagtgcactt > 2:202033/1‑232 (MQ=255) tAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTTTCGGGCGCCCGTTAGGCAT‑GCCCattat > 1:99860/1‑251 (MQ=255) aaaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTTTTTCGGgcgccccgtaggcattgccatttttgg > 1:531619/1‑228 (MQ=255) aaaCATGACGGGCCATTGTGATAGTGCGCCGGATCGGAAAACCGATCCCGGGCCTGCATTTGATTGGGCACGGTTTCGTGTGCGGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTGAGGCAT‑GACCATTATTGc < 2:96759/251‑1 (MQ=255) cGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCg < 1:441088/251‑1 (MQ=255) gtgATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑Cttttt < 1:412727/251‑1 (MQ=255) tGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑Gt < 1:385151/250‑1 (MQ=255) gcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAATTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTTCGGTGGGCGGGG‑GTTTTTTTCT‑Gtttcg > 1:137183/1‑248 (MQ=255) ggATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACgcgc < 1:18855/251‑1 (MQ=255) aTCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCAt < 1:149404/251‑1 (MQ=255) cGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCAtt < 1:372840/250‑1 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTCCGGCGCCCGGTAGGCCT‑GACCATTATTGCGATGGGCGGGC‑CTTTTTTTCT‑GTTACGCGCATTGC‑AGGGAgg > 1:66611/1‑249 (MQ=255) tCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGGCCT‑GACCCTTATTGCGGTGGGCGGGA‑CTTTTTTTCT‑GTTACGCGCATTGC‑CGGGGGTAtt > 1:512144/1‑251 (MQ=255) ccGGTCCTGCGTTTGATTGGGCACTGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTGTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGTGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATGGC‑AGGGAGTACTGt < 2:11841/251‑1 (MQ=255) cGGTCCTGCATTTGATGGGGCACGGTTTCGTGGGCGGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATGTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGtt < 2:66611/251‑1 (MQ=255) cTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTCGGGCGCCCGGTAGGCAT‑GACCATTATTTCGGTGGGCGTTG‑CTTTTTTACT‑GTTTCGCGCAATTC‑AGGGGGTATTGtttca > 1:552040/1‑246 (MQ=255) gCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGtt < 1:457904/251‑1 (MQ=255) tGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTATCTAGCGCACGTTAGGGAT‑GACCATTATTGCGCTGGGGGGGAACTTTTTTA‑T‑GTTAAGCGCATTGC‑AGGGCGTTTTGTTCCACGTTACCAc > 1:370225/1‑251 (MQ=255) gATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCAc < 1:281140/250‑1 (MQ=255) aTTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGTCAACATGACGTTATTTACAACCTTACTGGTGTTAATTTTCGAGCGGTTGGTTAAGGTGTGCGAGCACTGGCAGCTTTATCATCGTCTTGAAGTGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCTCGTTAGGCAT‑GACCATTATTGCGATGGGCGTGT‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGc < 1:39553/251‑1 (MQ=255) cACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACAATTTTTTTTCGGGCGCCCGTTAGGGATTGACC‑TTTTTGCGGTGGGCGTGG‑CTTTTTTTCT‑GTTTCGCGCCTTTC‑AGGGGGGATTGtttcacggtcccaacccccctg > 2:380544/1‑231 (MQ=255) ggTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGgtgt < 1:196099/251‑1 (MQ=255) gTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTCTTTCGGGCGCACGTTAGGGCT‑GACCATTTTTTCGGTGGGGGGGA‑CTTTTTTACT‑GTTACGCGCATTGCCAGGG‑GTATTGTTTAAACTTCCCCCGCTACTgggggggcggctggtttgt > 1:46709/1‑234 (MQ=255) aGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTg < 1:46839/251‑1 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑Ct < 1:81852/173‑1 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGGCCT‑GACCCTTATTTCGGTGGGCGTGA‑Ct > 2:81852/1‑173 (MQ=255) aGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCCGGCGCACGTTTGGCAT‑GACCATTATTTCGATGGGCGTGC‑CTTTTTTTCTTGTT‑CGCGCATTAC‑CGGGGGTATTGTTCAACGTTTCCACGCTTCCGGTGTGGGTGGTGATTGGTTTGCtgg > 1:116419/1‑250 (MQ=255) acaTGACGCTAGTTACAACCTTACTGGTGTTAGTTTTCGAGCGCCGGTTGAAGTGGGGCGAGCACTGGCAGCGTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgc < 2:60516/251‑1 (MQ=255) aaTGACGCTATTTACAAACTTACTGGTGTTAATTTTCGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGGCTTGAGGCGGTCTTTCGGGGGGTGAAACATTTTTCTCGCGGGCGCTCGTTAGGCAT‑GTCCATTATTGCTATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCTTTGC‑ATGTAGTGTTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgcg < 2:217348/250‑1 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTaa < 1:461281/249‑1 (MQ=255) aTTTACAACCTTACGGGTGTTAATTTTCGAGCTCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCTCACGTTAGGCAT‑GACCATTATTGCGATGTGCGGGA‑CTTTTTTACT‑GTGACGCGCAGTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTGCTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTTGCGCAGGTaa < 2:47035/249‑1 (MQ=255) ttACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTACGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAAAATTTTTTCTCTGGCGCACGTTAGGCAT‑GACCATTAATGCGCTGGGGGGGA‑CTTTTTTTCT‑GTTTCGCGCATTGC‑AGGGGGGAGTTTTCAACGGTTCCACGCTACTGGGGGGGCGTCTGCTTGGGTTTCTTTGTATTGTCGCAGGGAAAggt > 2:142635/1‑249 (MQ=255) aaCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGGCAT‑GACCCTTATTGCGGTGGGCGGGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGGGGATTGTTCAACGTTCCCCCGCCACTGGTGGGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGGAAAGTTCGTc > 1:462096/1‑251 (MQ=255) aCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTTCGATGGGCGTGA‑CTTTTTTTCT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCt > 1:6498/1‑251 (MQ=255) gtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCCCGTTAGGCAT‑GACCATTATTTCGGTTGGGGTGA‑CTTTTTTATT‑GTTACTCGCATTTT‑AGGGGGTATTTTTCAACGTTCCCACGCTACTTGTGTGGCTGCTGTTTGGTTTGTTGTGTATTGGCGCCGGGAAAGTTCGTCTTCAtttttatg > 1:390216/1‑245 (MQ=255) aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCtt < 1:515619/251‑1 (MQ=255) aTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACCTTTTTCTCTCCGGCGCACGTTAAGCAT‑GACCCTTATTGCGGTGGGCGGGA‑CTTTTTTTTT‑TTTACCCGCCTTGC‑AGGGGGGATTTTTTCACGTTTCCACCCTACCGGTGTGGCTGCTGATTGGTTTTTGGTGTATTGGGTCCGGGAAAGGTCGTCTTCATTTTCATggctt > 2:120249/1‑247 (MQ=255) aTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACAATTTTTTTTCGGGGGCACGCTAGGCAT‑GACCATTATTTCGATGGGGGGGA‑CTTTTTTACT‑GTTTCGCGCATTGG‑AGGGGGTATTGTTCAACGTTCCCACGCTACTGGTGGGGCTTCTGGTTGGGTTGCTGTGTATTAGCGCAGGGAAAGTTCGTCTTCATTTTCTTGCttt > 1:566834/1‑250 (MQ=255) cGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTg < 1:548553/250‑1 (MQ=255) ccTGTTTAAGTTGGGCGGGCCCTGGCAGCTTGATCATCGTCTTGAAGCGTTCGTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAgctg < 2:33578/251‑1 (MQ=255) ttAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTACGGCGGGGGAAACATTTTTTTCTCGGGCGCACGCTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑CGGGAGGATTGTTCAACGGTCCCACGCTACTGGGGTGGCTGCTGATTGGTTTGCTGTGTTTTTGCGCAGGGAAAGGTCGTCTTCATTATTCTGCTTATCTTACAGCCGCtttc > 1:316401/1‑249 (MQ=255) gTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTTCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTa < 1:515610/251‑1 (MQ=255) ttGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGAGATGGGCGTGA‑CTTTTTTTCT‑GTTTCGCGCATTGC‑AGGGGGGATTGTTCAACGTTCCCCCGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTTTTTGGGCAGGGAAAGTTCGTTTTCATTTTCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGcc > 1:443756/1‑251 (MQ=255) gATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCa < 2:390216/250‑1 (MQ=255) gATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCAc < 1:127193/251‑1 (MQ=255) gATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCAc < 2:531619/251‑1 (MQ=255) cgtcGTCTTGAAGCGTTCTTGCGGCGGGGGANACATTTTTCTCTCTGGCGCACGGTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGTCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGAt < 2:260388/249‑1 (MQ=255) gAAGCGTTCTTGCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCCTCATGATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGa < 1:184682/251‑1 (MQ=255) tCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGCGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTTTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGGCTTCATTATCATGCTTATCTTGCAGCTGCTTCACGTAATGATAGCCCTGCCCGTGCCACGATGGCTGGCGAACTCAcc > 1:265619/1‑251 (MQ=255) cggcggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGAt < 1:121737/251‑1 (MQ=255) ggcggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGAtt > 1:41740/1‑251 (MQ=255) cggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCa < 2:62697/251‑1 (MQ=255) gggagAAACATTTTTCTCTCGGGCGCACGATATGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTCCT‑CTTACGCGCATCGC‑AGGGAGTATTGTTCCACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCAGGCTTATCTGACAGCTGCTTCACGTAATGATATCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCAc > 2:543766/5‑251 (MQ=255) gTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACgg > 1:79848/1‑251 (MQ=255) aaaCATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGt < 2:137183/251‑1 (MQ=255) cctttttCTCTCGGGCGCACGGTAGGCAT‑GACCATTATTACGCTGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGA‑AGGGAGTATTGTTCAACGTTCACACGCTACTGGAGTGGCTGCTGATTGGATTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTCTCTGACAGCTGCTTCACGTAATGATAGCAATGCCCGTGCCACAGTGGCTGGCGACCTACCCATGATTCACGGCGGccc > 2:79245/3‑251 (MQ=255) | GATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCAT‑GACCATTATTGCGATGGGCGTGA‑CTTTTTTACT‑GTTACGCGCATTGC‑AGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCC > NC_000913/119156‑119639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |