Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,910,754	Δ300 bp		[mazF]	[mazF]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2910754	2911053	300	83 [0]	[0] 83	[mazF]	[mazF]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2910753	0 (0.000)	83 (1.150)	68/488	0.0	100%	intergenic (‑68/+3)	mazG/mazF	nucleoside triphosphate pyrophosphohydrolase/endoribonuclease toxin MazF
	?	NC_000913	2911054 =	0 (0.000)					coding (38/336 nt)	mazF	endoribonuclease toxin MazF

CCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAG  >  NC_000913/2910508‑2911000                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        ccAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcag                                                                                                                                                                                                                                                    <  1:387718‑M1/251‑6 (MQ=255)    gTTCTCCGCGAAGATCGTCAAAACCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGGGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTTAGCAAACGGTCGATTTGATTCATTGAATTGACCTGAAAATTGCGGGACTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatc                                                                                                                                                                                                                                                 <  2:372826‑M1/251‑9 (MQ=255)       cACCGCGAAGATCGTCAAAATCTTCACGGGGNATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcc                                                                                                                                                                                                                                              <  2:261857‑M1/251‑12 (MQ=255)             gcagaTAGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGAAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATAGCGCAGGCGCTGCATAATAGTGCGCAAACGGTCGATTTGATTCATTGCATTGTCCTGAAACTTGCGGGTCTGTCAGGTGGAAACCTGT‑ACACAGAATAGAAGTGAGTTATTAAatcaggtcgcccacatc                                                                                                                                                                                                                                        >  2:398996‑M1/3‑234 (MQ=255)             gAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAataagatcgcccatatc                                                                                                                                                                                                                                        >  1:347452‑M1/1‑234 (MQ=255)                    gTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacg                                                                                                                                                                                                                                 >  1:60674‑M1/1‑227 (MQ=255)                        aaaTCTTCACGGGCGATGGCGTCAAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatc                                                                                                                                                                                                                             >  1:511697‑M1/1‑223 (MQ=255)                                 cGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttacc                                                                                                                                                                                                                     >  1:217219‑M1/1‑214 (MQ=255)                                    gCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatagcgggtacgtatcggcttaccatta                                                                                                                                                                                                                 <  2:524142‑M1/251‑41 (MQ=255)                                     ggaTGGCTTCCAGCACTTCGTAGGTTTCTGCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTGTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGGGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccctatcgggtacgtatcggcttaccattac                                                                                                                                                                                                                <  2:471163‑M1/250‑42 (MQ=255)                                         ggCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccaga                                                                                                                                                                                                            <  1:52210‑M1/251‑46 (MQ=255)                                          gCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATGGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagac                                                                                                                                                                                                           <  1:339902‑M1/251‑47 (MQ=255)                                            gTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcgacttaccattaccagactt                                                                                                                                                                                                         >  1:293296‑M1/1‑203 (MQ=255)                                            gTCCAGCAATTCGTAGGATTCTTCAAGGGGGTAAGGCGCAATGGTGTCAAATTTCTGCTCTTTATCCCACGGGCAGCCGTTTTCAGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CCGACTAGAAGTGAGTTAGTAAatcagtacgaccatatcgggtacgtatcggcgtaccattcccagactt                                                                                                                                                                                                         >  2:400383‑M1/1‑203 (MQ=255)                                               cAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttcct                                                                                                                                                                                                      <  1:115844‑M1/251‑52 (MQ=255)                                                   aCTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatc                                                                                                                                                                                                  >  1:86849‑M1/1‑196 (MQ=255)                                                   aCTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatc                                                                                                                                                                                                  >  1:242581‑M1/1‑196 (MQ=255)                                                       cGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttc                                                                                                                                                                                              <  1:525080‑M1/251‑60 (MQ=255)                                                          aGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcgg                                                                                                                                                                                            <  1:481808‑M1/250‑62 (MQ=255)                                                           ggTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggct                                                                                                                                                                                          <  1:85740‑M1/251‑64 (MQ=255)                                                            gTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGAGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctc                                                                                                                                                                                         >  1:519666‑M1/1‑187 (MQ=255)                                                                        gtgtAAGGCGCAATGGGGACAAATGTCTGCTCTTTTTCCCACGCGCAGCAGTTTTCCGGATCGCGCAGGCGCTGCATACTAGTGAGCAAACGGTCGATTTGTTTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGGTAGGAAatcagatcgcccctatcgggtacgaatgggcttacccttaccagaactccttatctttcggctctccccagtcgat                                                                                                                                                                             >  2:441583‑M1/1‑175 (MQ=255)                                                                          gtaatgctcaatggtggCAAATTGCTTCTCTNTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CATAATAGAAGTGAGTTAGTAAatcagatcgcccatatcggttacgtttcggcttaccattaccagacttccttatctttcggctctccccagtcgatat                                                                                                                                                                           <  2:272670‑M1/243‑79 (MQ=255)                                                                            aaGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGTTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattc                                                                                                                                                                         <  2:382596‑M1/251‑81 (MQ=255)                                                                              ggCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTACGGATCGCGCAGGCGCTGCATAAAAGCGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGTAAACCTGAGAC‑CAGAATAGCAGAGAGTCAGTAAaacagctcgcccatatcgggaacgtaacggcttaccattaccagacttccttacctttcggttatccccagtcgatattctc                                                                                                                                                                       >  2:581900‑M1/1‑169 (MQ=255)                                                                               gcgcAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcg                                                                                                                                                                      >  1:494757‑M1/1‑168 (MQ=255)                                                                                            aaaTGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggcggttttccg                                                                                                                                                         >  2:311130‑M1/1‑155 (MQ=255)                                                                                            aaaTGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccg                                                                                                                                                         <  1:298715‑M1/251‑97 (MQ=255)                                                                                            aaaTGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccg                                                                                                                                                         <  1:429205‑M1/251‑97 (MQ=255)                                                                                                 tCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGACTTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGAAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcggg                                                                                                                                                    >  2:555543‑M1/1‑150 (MQ=255)                                                                                                    gCTCTTTATCCCACGGGCAGCTGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatg                                                                                                                                                 >  2:218830‑M1/1‑147 (MQ=255)                                                                                                    gCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGCTTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgt                                                                                                                                                                                                                                >  1:360059‑M1/1‑147 (MQ=255)                                                                                                    gCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTGCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGAC‑CCGAATAGAAGTGATTTAGTAAatcagatctcccatatcgggtacgt                                                                                                                                                                                                                                <  2:360059‑M1/172‑26 (MQ=255)                                                                                                          tATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttg                                                                                                                                           >  1:500008‑M1/1‑141 (MQ=255)                                                                                                           aTCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatc                                                                                                                                                                                                                                                      >  1:240017‑M1/1‑140 (MQ=255)                                                                                                           aTCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatc                                                                                                                                                                                                                                                      <  2:240017‑M1/143‑4 (MQ=255)                                                                                                             cccACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgacc                                                                                                                                        <  1:541669‑M1/251‑114 (MQ=255)                                                                                                             cacacGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCCAACGGTCGATTTGCTTCATTGAATTGTCCTGAAAATTGCGGATCTGTCAGGAGGTAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcaggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgagtatctcgtggaggttttccggcggtatgtcgttgacc                                                                                                                                        >  2:408093‑M1/3‑138 (MQ=255)                                                                                                                  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGTAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtccgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttc                                                                                                                                   >  1:250318‑M1/1‑133 (MQ=255)                                                                                                                  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttc                                                                                                                                   >  1:547121‑M1/1‑133 (MQ=255)                                                                                                                       gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaa                                                                                                                              <  2:547121‑M1/251‑124 (MQ=255)                                                                                                                         cgtgttccggATCGCGCAGGCGCTGCATAAGAGTGAGCAAACGGTCGATTGGAGTCATTGAATGGGCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagc                                                                                                                            <  2:250318‑M1/247‑126 (MQ=255)                                                                                                                             ttCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtctatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgta                                                                                                                         <  1:540572‑M1/250‑129 (MQ=255)                                                                                                                               ccGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattcccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaat                                                                                                                      <  1:147261‑M1/251‑132 (MQ=255)                                                                                                                               ccGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaat                                                                                                                      <  2:174826‑M1/251‑132 (MQ=255)                                                                                                                                        gcAGGAGCTGAATAATAGTGAGAAAACGGTCGATTTGATTCATTGAGTTGTCCTGCAAATTGCGAGTCTGTAAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggattacaattaccagacttccttatctttcggctctccccagtcgttattctcgtggaggttttccggcgtgatgtcgctggtcagttcaaaacgcgtaaatacggactct                                                                                                             >  2:421922‑M1/1‑111 (MQ=255)                                                                                                                                            gcgcTGTAGAATAGTGAGCAAACGGTCGATTTGATTAAGTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAATTTGGTTAGTAAatcagatcgcccatagcgggtacgtatcggttgaccattaccagacttccttatctttcggctctctccagtcgatatgctcgtggaggttttccggcttgatttcgttgaccagttcagcaagcgtaaatacgggctctttac                                                                                                         <  2:486482‑M1/251‑145 (MQ=255)                                                                                                                                                tGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGTAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcac                                                                                                     >  1:164421‑M1/1‑103 (MQ=255)                                                                                                                                                 gCATAATAGTGAGCAAACGGGCGATTTGAGTCATTGAATTGTCCTGAAAATTGCGGGTCGGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTTAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttactcact                                                                                                    <  2:86849‑M1/251‑150 (MQ=255)                                                                                                                                                   ataataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccctatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgcccagttcagcaagcgtaaatacgggctctttacgcacggg                                                                                                  >  2:410552‑M1/1‑100 (MQ=255)                                                                                                                                                      ataGTGAGCAAGCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGGCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctc                                                                                               <  2:415721‑M1/251‑155 (MQ=255)                                                                                                                                                      ataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctc                                                                                               <  2:6232‑M1/251‑155 (MQ=255)                                                                                                                                                      ataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctc                                                                                               <  1:226347‑M1/251‑155 (MQ=255)                                                                                                                                                           gAGCAAACGGTCGATTTGCTTCATTGCATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgcccagttcagcaagcgtaaatacgggctctttccgcactggctccata                                                                                           >  1:145939‑M1/1‑92 (MQ=255)                                                                                                                                                           gAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaata                                                                                           <  1:202522‑M1/250‑159 (MQ=255)                                                                                                                                                           gAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaata                                                                                           <  2:348790‑M1/250‑159 (MQ=255)                                                                                                                                                              cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataatt                                                                                        >  1:248404‑M1/1‑89 (MQ=255)                                                                                                                                                                      cGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgcc                                                                                >  2:223023‑M1/1‑81 (MQ=255)                                                                                                                                                                               tCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccag                                                                       <  2:97914‑M1/250‑179 (MQ=255)                                                                                                                                                                                    gAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaat                                                                 <  1:434704‑M1/251‑185 (MQ=255)                                                                                                                                                                                       ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtc                                                                                                                                                                                <  1:363227‑M1/137‑74 (MQ=255)                                                                                                                                                                                       ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtc                                                                                                                                                                                >  2:363227‑M1/1‑64 (MQ=255)                                                                                                                                                                                            cTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgcccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcactt                                                         >  2:209080‑M1/1‑59 (MQ=255)                                                                                                                                                                                              gAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTGAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattcccagactgccttatctttcggctctccccagtctatattctcgtagaggttgtccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttca                                                       <  2:176353‑M1/251‑195 (MQ=255)                                                                                                                                                                                                      ggggtCTGTCATGTGGAAACCTGTTAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccagatcgggttcgtatcggcttaccattaccaggcttccttatcgttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaat                                               <  2:500008‑M1/250‑203 (MQ=255)                                                                                                                                                                                                             gTCAGGTGGAAACCGGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatcttgcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattca                                          <  2:93657‑M1/249‑208 (MQ=255)                                                                                                                                                                                                               cAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagatt                                      >  2:192502‑M1/1‑40 (MQ=255)                                                                                                                                                                                                                 ggTGGAAACCTGTTAC‑CAGAATAGAACTGAGTTAGTAAatcatatcgcccatatcgggtaagtataggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgacccgttcagcaagcgtaaatacgggctctttacgcactggctcaataattactttgccatccaccagttccatcttcacttcctcctcaatattccgattg                                     >  2:250356‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                              gAC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcatta                        <  1:400383‑M1/250‑226 (MQ=255)                                                                                                                                                                                                                               aC‑CAGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaac                      <  1:218830‑M1/251‑228 (MQ=255)                                                                                                                                                                                                                                acaagaaTAGAAGTGATTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaac                      <  2:526323‑M1/248‑228 (MQ=255)                                                                                                                                                                                                                                   aGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgt                    >  1:12387‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                   aGAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttacttatctttcggctctccccagtcgatattctcgtggagggtttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgt                    >  1:106795‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                    gAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgtag                  <  1:577335‑M1/251‑232 (MQ=255)                                                                                                                                                                                                                                    gAATAGAAGTGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgtag                  <  1:396681‑M1/251‑232 (MQ=255)                                                                                                                                                                                                                                        aGAAGTGAGTTAGTAAatcagatagcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgtagccgg              >  1:512668‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                             tGAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgtagccgggatcc         >  1:148368‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                              gAGTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgctattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggccactcttcacttcatcatcaatattcagantgcgcgcctgcattaacgtagccgggatccg        >  2:254326‑M1/1‑10 (MQ=255)                                                                                                                                                                                                                                              gAGTTAGGAAaacagatcgcccatatcgggtacgtatcggctttccattaccaggcttccttatctgtcggctctccccagtcgatattctcgtggaggttttccgtcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcactgcatcatcaatattcagattgagcgcctgcagtaacgtagccgggatccg        <  2:494757‑M1/251‑242 (MQ=255)                                                                                                                                                                                                                                                gTTAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcctcaatattcagattgagcgcctgcattaacgtacccgtgatccgcc      >  2:13352‑M1/1‑8 (MQ=255)                                                                                                                                                                                                                                                  tAGTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgtagccgggatccgcacc    >  1:185468‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                                                    gTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatcttctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatcttcagattgagcgcctgcattaacgtacccgggatccgcaccgc  >  2:409174‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                                                    gTAAatcagatcgcccatatcgggtacgtatcggcttaccattaccagacttccttatctttcggctctccccagtcgatattctcgtggaggttttccggcgtgatgtcgttgaccagttcagcaagcgtaaatacgggctctttacgcactggctcaataattaatttgccatccaccaggtcaatcttcacttcatcatcaatattcagattgagcgcctgcattaacgtagccgggatccgcaccgc  >  1:497978‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        CCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAC‑CAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAG  >  NC_000913/2910508‑2911000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑