Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,139,511	Δ1 bp	coding (198/663 nt)	fsaB ←	fructose‑6‑phosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,139,511	0	C	.	100.0%	250.2 / NA	57	coding (198/663 nt)	fsaB	fructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/29);  total (28/29)

CTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGT  >  NC_000913/4139265‑4139760                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          cTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑Tgcgc                                                                                                                                                                                                                                                      >  1:408314/1‑251 (MQ=255)   ccTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑Tgcgc                                                                                                                                                                                                                                                      <  1:221650/249‑1 (MQ=255)              cGAATGCCGTTTCCGCCCTGGGCATCTACGCGGGTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCCCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATg                                                                                                                                                                                                                                         >  2:389166/1‑251 (MQ=255)                 aTGCCGTTTCCGCCCGGGGCATTTACGCGGTTAACAAACGGAGCAACGTAGTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACATCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATATCGTCGCGCAGGCGCTGCGCTTCTTCCATCATCCC‑TGCGCGTCGCGGCTCATGGTc                                                                                                                                                                                                                                      <  2:350998/251‑1 (MQ=255)                            gCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAAc                                                                                                                                                                                                                             >  1:432719/1‑249 (MQ=255)                             cccTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGCAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAAc                                                                                                                                                                                                                             >  1:319698/1‑248 (MQ=255)                             cccTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAG‑AGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGa                                                                                                                                                                                                                          >  2:435233/1‑250 (MQ=255)                                 gggCATCTACGCGGTTAACATACGGAGCAACGTATTTTTCCCCTGCCAGTGCGGCTAATATCCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGc                                                                                                                                                                                                                      <  1:95672/251‑1 (MQ=255)                                    gcacgtcagatctAACATAAGGATCAACGTATTTTTCCCCTGCCAGTGCGGCGAATACCCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGGAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTAGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑GGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCt                                                                                                                                                                                                                   <  2:318794/239‑1 (MQ=255)                                         aCGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCtc                                                                                                                                                                                                                  <  1:413042/247‑1 (MQ=255)                                            cgGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCa                                                                                                                                                                                                           <  1:496272/251‑1 (MQ=255)                                               ttAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCTTGGTCTGAGCAAACAGAATGCCCTCATCACCAATc                                                                                                                                                                                                        >  2:549596/1‑251 (MQ=255)                                                 aaCATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTACTACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGCAAGTCACCGGTATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCAC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCt                                                                                                                                                                                                                   >  1:318794/1‑238 (MQ=255)                                                  aCATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCt                                                                                                                                                                                                     <  1:334038/251‑1 (MQ=255)                                                       cggtgCAACGAATTTTGCCCCTGCCAGGGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTTGTAATGCCCTCTTGTTTCAGGTGTTTAATTGCTGCCATACCTTCGGAAGTCAGCGGGATGTTCTCCACAATTCCCTGAATAGCGGCTCGCAGGCGCGTCGCTTCGTCCACCATCCC‑TGCGCGTCGCGGCTCATGTTCTGAGCAAACTGAATGCCCTCAGCTCAAGTCGCttttt                                                                                                                                                                                                 <  2:518467/246‑1 (MQ=255)                                                                gTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCgg                                                                                                                                                                                       <  2:186151/251‑1 (MQ=255)                                                                           cTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTATTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCGTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGAAGACGCGGCAGCCCTTccc                                                                                                                                                                            >  2:467108/1‑251 (MQ=255)                                                                             gCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGCCGCGGCCGCACTTCCCat                                                                                                                                                                          >  2:188828/1‑251 (MQ=255)                                                                             gCCAGTGCGGCTAATAACCCTTGAGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGGAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCCATCGCTTTTTGCCGACGCGGCCGCCCTTCCCat                                                                                                                                                                          >  2:205580/1‑251 (MQ=255)                                                                                 gTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATgg                                                                                                                                                                      <  1:188828/251‑1 (MQ=255)                                                                                      gCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGAATcc                                                                                                                                                                 >  1:137727/1‑251 (MQ=255)                                                                                       cTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGcctcct                                                                                                                                                                >  2:14610/1‑251 (MQ=255)                                                                                         aataaCCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTAACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTg                                                                                                                                                              >  1:515941/1‑251 (MQ=255)                                                                                              cccTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGc                                                                                                                                                         <  1:340360/251‑1 (MQ=255)                                                                                                       cgcgcTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAAt                                                                                                                                                <  1:63565/250‑1 (MQ=255)                                                                                                                  cagcagTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTTCTGGCAGCGATAATGCTCGGGtt                                                                                                                                       >  1:205813/1‑249 (MQ=255)                                                                                                                    gcagTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGtt                                                                                                                                   <  1:474675/251‑1 (MQ=255)                                                                                                                      agTGCCAAGAGTAGTCATACCCTCTCTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCCCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCACTCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCNTGCTGGCAGCGCTAATGCGCGCGTTAGTTGt                                                                                                                                 >  2:281352/1‑251 (MQ=255)                                                                                                                           cAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTAGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCCGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATACTGCTCGGGTTAGTTGTCAccc                                                                                                                            >  1:32665/1‑251 (MQ=255)                                                                                                                            aaGTGTATTAATACCCTCTTTTTTCAGTATTNTAATTGCTGCCAGACCTGCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCAcccc                                                                                                                           <  2:272174/251‑1 (MQ=255)                                                                                                                             aGTGTAGAAATCCACTCTTTTTTCAGTATTTTAATTGCTGCCCGACCTTCGGAAGTCACCGGGCTTTTCACCACACTACCCCGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGAGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGCCAGCACTTCCCATATGGACTCCTGGCTGGCAGCGATGATACTCGGGTTTGTTGACAACCCg                                                                                                                          >  2:374868/1‑251 (MQ=255)                                                                                                                                   gtaATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTGCACCACAATTCCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATg                                                                                                                    <  2:434046/251‑1 (MQ=255)                                                                                                                                              ttttttCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGTCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCCGCACTTCCCATATGGACTCCTTGCTGGCCGCGGTCATGCTCGGGTTAGTTGTAACCCCGTCTATGGGGACTATGCg                                                                                                         >  2:196809/1‑251 (MQ=255)                                                                                                                                                     aGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCATTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGcacgc                                                                                                  >  2:526114/1‑247 (MQ=255)                                                                                                                                                           ttAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCg                                                                                            >  1:105534/1‑251 (MQ=255)                                                                                                                                                              aTTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACt                                                                                         >  1:152437/1‑251 (MQ=255)                                                                                                                                                               ttGCTGCCAGACCTTCGGAAGACACAGGGATTTTCACCACAATACCCGGAATAGAGGCGCGAAGGCGATTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGACTGAGCAAACAGAATTCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGAATCCTTGCTGGCAGAGATAATGCTCGGGTTAGTATTCCCCCCGGCAATGGGGACTATTGGTGCCAGAAGttggaatt                                                                                        >  2:387147/1‑245 (MQ=255)                                                                                                                                                                          ccTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGtt                                                                              >  1:232378/1‑250 (MQ=255)                                                                                                                                                                             tCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGc                                                                           <  2:408314/250‑1 (MQ=255)                                                                                                                                                                               ggAAGTCACCGGGATTGTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGTTAATATGCTTGCCAGACGTTCTACTTCTGCGTCGTTAGCGgt                                                                        <  1:178643/251‑1 (MQ=255)                                                                                                                                                                                   atCACCGGGATTTTCACCACAATTCCCGGAATAGCGGCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTGTTGCAGACGCGGCAGCACTTCCCATATTGTCGCCTTGCTGGCAGCGATAATGCTCGGGTGAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCAGTGTcc                                                                    <  1:362233/250‑1 (MQ=255)                                                                                                                                                                                           gATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCCATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATAc                                                              >  1:82527/1‑249 (MQ=255)                                                                                                                                                                                                      caATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGt                                                 <  1:281983/251‑1 (MQ=255)                                                                                                                                                                                                        aTACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGCCTCCTTGTTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCACTGGGGCATATGCGTGCCAGCCGTTCGACTTCTGCGACGTGAGGGGTGTCCAGATACAGTTCCataatgatt                                               >  2:442353/1‑248 (MQ=255)                                                                                                                                                                                                        aTACCCGGAATAGCGGCTCGCAGGCGCTTCGCTTCTTCCCCCCTCCC‑TGCGCGGCGCGGCTCATGGTCGGAGCTAACAGAATGCCCTCATCTCCAATCGCTTTTTGCAGACGCGGCAGCATTGCCCATATTGCCTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGttt                                               <  2:500816/251‑1 (MQ=255)                                                                                                                                                                                                          aCCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTc                                              <  1:227649/250‑1 (MQ=255)                                                                                                                                                                                                           cccGGACTAGCGGCGCGCAGGCGCTTCGGTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTGAGCGGTGTCCAGATACAGTTCCATGATGTTTCCt                                            <  2:376370/251‑1 (MQ=255)                                                                                                                                                                                                             cGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCttt                                          <  1:435233/251‑1 (MQ=255)                                                                                                                                                                                                              ggAATAGCGTCGCGCAGGCGATACGCTTCATCCACCATCCC‑TGCGCGGCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTGTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCGGGCAGCGATAATGCTCGGGGTAGTTGTCACCCAGGCAATTGGGAATATGCGTGCCAGACGTGCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCttta                                         <  2:171689/251‑2 (MQ=255)                                                                                                                                                                                                                   aGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTGCCATGATGTTTCCTTTTAcag                                     <  1:181678/250‑1 (MQ=255)                                                                                                                                                                                                                       tCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTAcagttccc                                >  2:205010/1‑250 (MQ=255)                                                                                                                                                                                                                        cgcgCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTAcagttcca                                <  1:228173/250‑1 (MQ=255)                                                                                                                                                                                                                            cAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTAcagttccagttc                            >  1:514425/1‑250 (MQ=255)                                                                                                                                                                                                                             aGGCGCTTCGCTTCTTCCCCCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCGCATCACCAATCGCTTTTTGCAGTCGCGGCAGCACGTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCAt                          <  2:154341/251‑1 (MQ=255)                                                                                                                                                                                                                             aGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACCGTTCCAGTTCAt                          >  1:98484/1‑251 (MQ=255)                                                                                                                                                                                                                                cgcttcgcttCTTCCACCATCCC‑GGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGtt                       <  1:111439/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcttcgcttcTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTg                      <  1:175279/251‑1 (MQ=255)                                                                                                                                                                                                                                             ccaccaTCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGc          <  1:14610/251‑1 (MQ=255)                                                                                                                                                                                                                                              caccaTCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCg         >  1:190112/1‑251 (MQ=255)                                                                                                                                                                                                                                                     cccTTCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCGTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCCCTGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCTACGTTAGCGGTGTCCAGATACAGTGCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCTATAGc    <  2:332517/250‑1 (MQ=255)                                                                                                                                                                                                                                                      ccTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGAGATAGCGt  >  2:476907/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          CTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGT  >  NC_000913/4139265‑4139760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑