Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,307	G→A	L154L (CTC→CTT)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,307	0	G	A	100.0%	219.1 / NA	59	L154L (CTC→CTT)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/26);  total (33/26)

GGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTT  >  NC_000913/3815062‑3815551                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ggCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCg                                                                                                                                                                                                                                                  >  1:386579/1‑250 (MQ=255)     gCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATc                                                                                                                                                                                                                                             >  1:90562/1‑251 (MQ=255)          ttGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGATTTTTTTTAAACCCCAACCTCTTCGCGAGAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGTGAAATCaata                                                                                                                                                                                                                                         <  2:389432/250‑3 (MQ=255)              ggCACAGTTTCATTAATATGACGCCGGTTGTCTTTTCATCCGGCGAGTTGCTTTAAACGCCAAACTCTTCGCGATAGTCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCATTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCTCAATCAATACGcct                                                                                                                                                                                                                                    <  2:76479/251‑2 (MQ=255)                    gttacAGTAATAGGACGCCGGATGTCTTTTCNCCCGGCGAGTTTCTTTAAACGCCAAACTCGTCGCGATAGGCCTTATCCGCCGCCAGTTTTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCTCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCatgc                                                                                                                                                                                                                              <  2:267723/247‑4 (MQ=255)                    gTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGc                                                                                                                                                                                                                              <  1:220301/251‑1 (MQ=255)                        cAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCCATACGCCAGCAAGCGTcg                                                                                                                                                                                                                          >  2:7720/1‑251 (MQ=255)                           tAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGCCGATCAAGCGAAATCAATACGCCAGCAAGCGCCgcgc                                                                                                                                                                                                                       >  2:118606/1‑251 (MQ=255)                            aagatGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACCCCAACCTCTCCGCGATAGGCCTTAACCGCCGCCAGATGTGCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGcc                                                                                                                                                                                                                      <  2:172262/248‑1 (MQ=255)                            aaTATGACGCCGGATTACTTTTCATCCGGCGAGTTTCTTTAAACACCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTcgtgc                                                                                                                                                                                                                       <  2:439243/250‑4 (MQ=255)                                    gCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCt                                                                                                                                                                                                              >  1:393086/1‑251 (MQ=255)                                        gATGACTTTTCAGCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTGCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGaat                                                                                                                                                                                                          <  1:169604/251‑1 (MQ=255)                                                   aTCCGGCGAGTTTCTTTAAACCCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCg                                                                                                                                                                                               <  2:111938/251‑1 (MQ=255)                                                         cGAGTTTGTTTAAACCCCAAACTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTGCTCTTCCAGATAGGCAATCAGAGCTTTCATGGTTATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTcgc                                                                                                                                                                                         <  1:298208/251‑1 (MQ=255)                                                               tCTTTAAACGCCAAACTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATc                                                                                                                                                                                    <  2:386579/250‑1 (MQ=255)                                                                  ttAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGCAATCAATACGCCAGCAAGCGTCGCGCCATTAACCTGAATAATCTCCATCGATTCGCGAATCGCCg                                                                                                                                                                                >  1:18711/1‑251 (MQ=255)                                                                  ttAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCg                                                                                                                                                                                >  1:104023/1‑251 (MQ=255)                                                                            aaCTCTTCGCGATAGTCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGt                                                                                                                                                                      <  1:201112/251‑1 (MQ=255)                                                                                ctTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGCGATc                                                                                                                                                                  >  2:16742/1‑251 (MQ=255)                                                                                          ggCCTTATCCGCCGCCAGATGTTCCGCCAGTTCCGGCTGCTCTTCCAGATAGGTAATCCGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAAGCGCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCAcctcatata                                                                                                                                                        <  2:206380/251‑4 (MQ=255)                                                                                                  ccgccgCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCAt                                                                                                                                                                                                                    >  1:22594/1‑183 (MQ=255)                                                                                                  ccgccgCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTCCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCAt                                                                                                                                                                                                                    <  2:22594/183‑1 (MQ=255)                                                                                                     ccgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg                                                                                                                                             <  1:108668/251‑1 (MQ=255)                                                                                                         cAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTc                                                                                                                                         >  1:56262/1‑251 (MQ=255)                                                                                                          aGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATACTCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCCCATCCTCTCCCAGccttccccgccc                                                                                                                                        >  2:159385/1‑240 (MQ=255)                                                                                                              gTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtctc                                                                                                                                                                                                    >  1:260480/1‑187 (MQ=255)                                                                                                              gTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtctc                                                                                                                                                                                                    <  2:260480/187‑1 (MQ=255)                                                                                                               ttCCGCCATTTCCGGCTTGTCTTCAAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGCTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGACGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGCCGCGCCATTAGCCTGAATAATATCCATCGATTCGCGACTCGCCGTTCCGGCGGTGACCACCTCATCTACCAGCATTACGCGTCCTTGtc                                                                                                                                   >  2:454800/1‑250 (MQ=255)                                                                                                                       tttCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCTCAAATCGCCGTTCCGGCGGTGATCCCATCATCTACCAGCATTACGCCTCCTTGTAACGGGCtc                                                                                                                           >  1:81965/1‑250 (MQ=255)                                                                                                                            ggCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGCGATCACATCATCTAACAGCATTACGCGTCCTTGTAACGCGCTaccaac                                                                                                                      >  2:346883/1‑251 (MQ=255)                                                                                                                                 ctctTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCCGTTGTAATCACCCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCAATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCAGTACGCGTCCTTGTAACGCCCTACCAACCCGAt                                                                                                                 >  1:529821/1‑251 (MQ=255)                                                                                                                                     gccagataggCAATCAGATCTGTCAGGGTGATGATAGAGATCACTTTGCAGTGGTATTCACGCTCATCTCCCTGAATCGCCGAAATCCCGCCGCGCCCCCGTTCCTGACGATCAAGCGAACTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAACCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTCACGCGCTACCAACCAGAtt                                                                                                                <  1:231663/239‑1 (MQ=255)                                                                                                                                      ccagataggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATCACATCATCTACCCGCATTACGCGGCCTTGTAACCCGCTCCCAACCAGATTgccc                                                                                                            >  2:26173/9‑250 (MQ=255)                                                                                                                                             ggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCAc                                                                                                     >  1:352733/2‑251 (MQ=255)                                                                                                                                              gCAAACAGATCTCTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTGCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTCCCTGATGATCAAGAGAAATCAAGGCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGTTGATCGCATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCATGTTGCCGCCGTCAcc                                                                                                    <  2:229767/251‑1 (MQ=255)                                                                                                                                                        cTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGAGGTCtttt                                                                                          >  1:86325/1‑251 (MQ=255)                                                                                                                                                                gTGATGATAGAGATCACTTTGCAGTTGTAATNCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCttt                                                                                  <  2:242777/251‑1 (MQ=255)                                                                                                                                                                   atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCg                                                                               >  1:348982/1‑251 (MQ=255)                                                                                                                                                                   atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGAGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCg                                                                               >  1:305713/1‑251 (MQ=255)                                                                                                                                                                     gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCCCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATATACCAGCATTACGCGTCCTTGTAACGCGCTACCCACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                             >  1:536602/1‑251 (MQ=255)                                                                                                                                                                         gagaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTaa                                                                          >  1:249094/1‑250 (MQ=255)                                                                                                                                                                          agaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGTTTAAAg                                                                        <  1:477194/251‑1 (MQ=255)                                                                                                                                                                            aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCTATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCa                                                                      <  1:481802/251‑1 (MQ=255)                                                                                                                                                                            aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGc                                                                       >  1:142879/1‑250 (MQ=255)                                                                                                                                                                                cTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTAc                                                                  >  1:282294/1‑251 (MQ=255)                                                                                                                                                                                 tttGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGt                                                                                                  >  1:186298/1‑218 (MQ=255)                                                                                                                                                                                 tttGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGt                                                                                                  <  2:186298/218‑1 (MQ=255)                                                                                                                                                                                      aGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGCACGGcagg                                                            >  1:214732/1‑251 (MQ=255)                                                                                                                                                                                       gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGACGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAAACAGATTGACGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCaccacgcacggg                                                           >  2:57435/1‑240 (MQ=255)                                                                                                                                                                                          gTAATCCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTACAGCAGTACGGcaggtcaa                                                        <  2:451097/251‑3 (MQ=255)                                                                                                                                                                                                          tCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGGACGGcagggtaaaggcatgttttccccc                                        >  2:333036/1‑231 (MQ=255)                                                                                                                                                                                                             tGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCCGGACGGcaggtcgcggtcttgcttcttcccccg                                     >  2:380996/1‑230 (MQ=255)                                                                                                                                                                                                              gAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAATCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGGTAAAGCAGTACGGcagggaaagggcctgattctccccccgc                                    >  2:328278/1‑227 (MQ=255)                                                                                                                                                                                                                aTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATACTccccccgcgc                                  >  2:462967/1‑243 (MQ=255)                                                                                                                                                                                                                   gCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAACCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCcac                               <  2:87614/251‑1 (MQ=255)                                                                                                                                                                                                                             cgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCAGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtag                       <  2:393086/249‑3 (MQ=255)                                                                                                                                                                                                                                  cgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCATGATGCTCCGCCAGCGCCAcggcgggagggggcgc                >  1:92318/1‑241 (MQ=255)                                                                                                                                                                                                                                        cgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCgg          <  2:92318/251‑1 (MQ=255)                                                                                                                                                                                                                                                aCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCtt  <  1:57513/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTT  >  NC_000913/3815062‑3815551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑