Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,795	G→A	Q128* (CAG→TAG)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,795	0	G	A	100.0%	92.5 / NA	28	Q128* (CAG→TAG)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/8);  total (20/8)

CCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTG  >  NC_000913/2481551‑2482022                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     ccTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAg                                                                                                                                                                                                                                <  1:23062/251‑1 (MQ=255)            cATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACg                                                                                                                                                                                                                    >  2:221738/1‑251 (MQ=255)            cATTTTGACCGGGGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTGTTTT‑CTGGCGGATAATTCCGTAATAATAAACTCTATGACAGTGGAA‑TTAAc                                                                                                                                                                                                                     <  2:23427/250‑1 (MQ=255)                                 aGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCGCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGa                                                                                                                                                                                                >  1:293203/1‑250 (MQ=255)                                  gTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTaaatcatgaac                                                                                                                                                                                              >  2:392578/1‑242 (MQ=255)                                             ttaaagaaagGTAAAGAATAATGACAGGACAAAAACCCCCATGGGAACTTTGAAAAAAAATACCCACCCCCAGAAAAAGTTACCAAAAATATCCCCCCCCAATATCGGCCCAAATGTCGGGGCGATAATCACGGTCTTTGCCCA‑TAA‑GCCAAGAAAAATTTTTTTTTTTT‑TTGGGGAATAATTCTTAAATAAAAACCTCTAGGACATTGGAA‑TTACCGCCCCCGCCATTAACCCCTGAACGACTCTaaaa                                                                                                                                                                                    <  1:410929/243‑1 (MQ=255)                                                   aaagGTTAAGCATAACGTCACGACGCTAATCCCCATCGGGACATTGATTAAAAATATCTAACCCCAACTAAAGTTATCACAAATATAACCTCGCAATATCGGCCCACATATCGGAGCGATAATCACGGACATTGACCA‑TAATGCCA‑GAGCAAATATGCTGTTTT‑CTGGCGGATAAATCATTAATAAGAAACTATATGACAGTGGAA‑ATAACGGCCCCTCCCTTTACCCCCGCAAGACTCTAAAAAataac                                                                                                                                                                              >  2:428785/2‑248 (MQ=255)                                                                aaTGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                                                                                                                                                >  1:142055/1‑251 (MQ=255)                                                                   gtcagaactaaaaacccaaaaggaaCATTAATAAAAAATACCCACCCCCATCAAAATTTATCAAAAATAAACCCGCCAAATACCGGCCCACATATCGGAGAGTAAATCACTTTTTTTGCCCA‑TAATGCCA‑GAAAAATTTTTCTTTTTT‑CTGGCGAATATTTCTTTAAAAAAAAACTCTATGACAGTGAAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                                                                                                                                             <  1:94403/227‑1 (MQ=255)                                                                           gATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATCGGc                                                                                                                                                     >  1:492397/1‑251 (MQ=255)                                                                            aTAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCt                                                                                                                                                    >  1:248884/1‑251 (MQ=255)                                                                             tAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCAAATCAAGATTGGTCGATAGGCt                                                                                                                                                    >  1:395497/1‑250 (MQ=255)                                                                                              ttGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCTATAGGCTACACATTAATGAAGACa                                                                                                                                   >  2:326550/1‑250 (MQ=255)                                                                                                gATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAAc                                                                                                                                                                                                                     >  1:362240/1‑166 (MQ=255)                                                                                                gATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAAc                                                                                                                                                                                                                     <  2:362240/166‑1 (MQ=255)                                                                                                              accccaagcaaaatttATCACAAATAAACCCCCCCAATACCGGCCCACATAGCGGAGCGATAATCACGTTCTTTGCCCA‑AAATGCCA‑GAGCAATTTTTTTTTTTT‑CGGGGGAATAATTCCTAAAAAAAAAACTCTAGGACAGTGGAA‑TTAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACt                                                                                                                  <  1:358752/237‑1 (MQ=255)                                                                                                              aaCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACt                                                                                                                  >  2:313268/1‑251 (MQ=255)                                                                                                                          aGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaat                                                                                                      >  1:286085/1‑251 (MQ=255)                                                                                                                               tCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCt                                                                                                 >  1:34506/1‑251 (MQ=255)                                                                                                                                      aataccccccaaaTACCGGCCCACATAGCGGAGCAATAACCACGGTCATT‑ACCAATAAGGCCA‑GAAAAAATGTTTTTTTTT‑CGGGAGAATATTTCATAAAAAAAAACCTCTATGACAGAGAAA‑TTAGCGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                                                                                          <  1:313268/240‑1 (MQ=255)                                                                                                                                       ataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                                                                         >  1:429025/1‑251 (MQ=255)                                                                                                                                          aCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑ATGGCGGCTAATTCCTTAATAATACACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAAACATTAATGAAGAAAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGcat                                                                                       >  2:499094/1‑248 (MQ=255)                                                                                                                                              ccaaatacacccccACATAGCGGAGAAATAATCACGTTC‑TTGACCAATAATGC‑A‑AAAAAAATTTTTTTTTTTGCTGGGGGATAATTCATAAATAAAAACCTCTATGA‑AATGGAAATTAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCt                                                                                  <  1:326550/240‑1 (MQ=255)                                                                                                                                                 aaTATCGGCCCACATATAGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtg                                                                               >  1:414980/1‑251 (MQ=255)                                                                                                                                                                           aaTCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCg                                                     >  1:167944/1‑251 (MQ=255)                                                                                                                                                                                                          cAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATCAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGACGATAGGCTAAACATTAATGAAGACAACGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGGAACAGGGATCGCAATGGCCTTTGCTACACCAAACGAGGTg                        >  2:130253/1‑251 (MQ=255)                                                                                                                                                                                                          cAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCCCCGCCCTTAACCCCTGAACGACTCTAAAAAATATCAACACATCCAGATTGGTCGATAGGCTACAAATTAATGAAGACAGCGAAAAACAAGTGACTGAAAGTAAAAATAATCTTACTTCGCCTATTCTTTGTGCAAACCTGCCAGTAACAGGGANCGCAATGGCATTTGCTACACCAAACGCGGTg                        >  2:271947/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTAATTCCTTAATAATAAACTCTATGACAGTGGAA‑TTAACGGCTCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTTCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTg  >  1:292347/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     CCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCA‑TAATGCCA‑GAGCAAATGTTCTTTTTT‑CTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTG  >  NC_000913/2481551‑2482022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑