Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,586	G→A	Q249Q (CAG→CAA)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,586	0	G	A	100.0%	225.6 / NA	63	Q249Q (CAG→CAA)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/44);  total (19/44)

GCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGC  >  NC_000913/2251339‑2251826                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGACCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCCATATCGGTCACGATGCGTTCCGCTGGATCATGCAAgac                                                                                                                                                                                                                                               >  1:23232/1‑251 (MQ=255)    gggAAGATAACTCCCGCGGCGGCGTCTGCATTGAGACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGTAGCATGCAAgacgac                                                                                                                                                                                                                                            <  2:305318/250‑1 (MQ=255)     ggAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCCCCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGAcc                                                                                                                                                                                                                                           <  1:202706/251‑1 (MQ=255)         gATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGAATCTGCGCGGGATGCACCTTAACGATGCGAAACGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGGGAAGGCAATATCGGTCATGATNCGTTCCGCGGGACCCTGCAAGACGCCCGttt                                                                                                                                                                                                                                       >  2:252814/1‑251 (MQ=255)            aaaTCCCGGGTCGGCGTCTGCTTTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg                                                                                                                                                                                                                                     <  2:4032/250‑1 (MQ=255)            aaaTCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa                                                                                                                                                                                                                                    <  1:74888/251‑1 (MQ=255)             aaTCCCGTGTCGGCGGTTGCATTGATACCTGCCATGCTTTCGCTGCCGTGTATGATTGGCGTACTCCAGCCGAATGCGAGAAAACATCCGCGGATTTTTCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCCCTTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTTAAGGCAATATCGGTCATGATGCTTTCCGCTGGATCATGCAAGACGACCGTTTCGAc                                                                                                                                                                                                                                   <  1:516591/251‑1 (MQ=255)             aaTCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGCCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGAACGTTTCGAc                                                                                                                                                                                                                                   <  1:523852/251‑1 (MQ=255)                 ccGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGc                                                                                                                                                                                                                                >  2:45642/1‑250 (MQ=255)                               gCATGGATACCTGCCTTGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAGCATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCTCCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCTCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTGCGCTGATcc                                                                                                                                                                                                                   <  2:308851/249‑1 (MQ=255)                                cATTGATCCCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCCCCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCg                                                                                                                                                                                                                <  1:122056/251‑1 (MQ=255)                                cATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCg                                                                                                                                                                                                                >  1:488207/1‑251 (MQ=255)                                   gtcacccTGCCATGCTTCCGCGGCCGGTTATGTTTGGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCGGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATAGCGGTCATGATGCGTTCCGCTGGATCATGCAAGTCGACCGTTTCGACGGCATTCCGCTGAGCCTCGaa                                                                                                                                                                                                              <  2:191946/245‑1 (MQ=255)                                      cacatgccttgttttCGCTGCAGGTTATGATTTGCGGACTCCAGCCGAAGGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGTGGGATGTACCTTAACGATGAGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCa                                                                                                                                                                                                          <  2:533151/239‑1 (MQ=255)                                        ccTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc                                                                                                                                                                                                        <  1:38297/251‑1 (MQ=255)                                                   ttCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCCCCTTAACGATGCGAAAAGCCCCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGat                                                                                                                                                                                               <  1:41094/249‑1 (MQ=255)                                                      gCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTg                                                                                                                                                                                          >  1:286203/1‑251 (MQ=255)                                                                               cAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGaaa                                                                                                                                                                 <  1:468594/251‑1 (MQ=255)                                                                                      aggCGAGAAATCCTTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGACTGGCTGAAAGCGcaac                                                                                                                                                          <  2:386849/249‑1 (MQ=255)                                                                                           agaAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGCTGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACt                                                                                                                                                     >  2:68897/1‑251 (MQ=255)                                                                                             aaaaCATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTg                                                                                                                                                    >  1:6528/1‑250 (MQ=255)                                                                                                       cgGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCAACTTCACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGACGGCAATATCGGTCATGATGCGTTCAGCTGGCACATGCAAGGCGACCGTTTCGACGGCATTACGCTGATCCTCGAAACCATAAACCCGGATATCTGGGCAGCAGAGATCGCTCNGCTGAAAGCGCAACAAACTGAACACGCGGtg                                                                                                                                         >  2:265649/1‑250 (MQ=255)                                                                                                        ggagtttGCCCGTACTGTCGGCTTTAAGTATCGGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGAGCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAg                                                                                                                                        <  1:194267/247‑1 (MQ=255)                                                                                                          aTTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGcc                                                                                                                                      >  1:82441/1‑251 (MQ=255)                                                                                                              tGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGa                                                                                                                                   >  1:83671/1‑250 (MQ=255)                                                                                                                        ggcggCTTTAAGTATTTGCGCGTGATGCACCGTAACGATGCGACAAGCACCTTTGGCAGCCGCGTTGCCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGAAGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgt                                                                                                                          <  2:362512‑M1/247‑7 (MQ=255)                                                                                                                             cTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGCCGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGCTATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctgg                                                                                                                   >  2:311930‑M1/1‑238 (MQ=255)                                                                                                                                tAAGTATATGCGCGGGCTGCACCTTACCGATGCGAACAGCACATTTGTCAGCCGAGTTGACCGCCATCATCGCCTAGGCGAAAGCACTCTAGGTCATGATGCGTTCAGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTCCTCCTCGAAACCATCAACCCGGATCTCTGGGCCGAAGAGATACCCTGGCTGGACGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGagcacgtcatctggaa                                                                                                                 >  2:474272/1‑235 (MQ=255)                                                                                                                                       cTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcc                                                                                                                        >  2:378402‑M1/1‑228 (MQ=255)                                                                                                                                       cTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcc                                                                                                                        <  1:378402‑M1/236‑9 (MQ=255)                                                                                                                                           gcgGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaa                                                                                                     <  1:423288‑M1/251‑28 (MQ=255)                                                                                                                                            cgGGATGCCCCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaac                                                                                                    <  1:357007‑M1/251‑29 (MQ=255)                                                                                                                                            cgGGATGCACCTTAACGATGCGAAAAGCCCCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaac                                                                                                    <  1:392760‑M1/251‑29 (MQ=255)                                                                                                                                             gggATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGTCCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaacc                                                                                                   <  1:252814‑M1/251‑30 (MQ=255)                                                                                                                                              ggATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCCTTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccc                                                                                                  >  1:494939‑M1/1‑221 (MQ=255)                                                                                                                                                aTGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctg                                                                                                <  2:166490‑M1/251‑33 (MQ=255)                                                                                                                                                      cctaaCGATGCGAAAAGCCCCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatc                                                                                          <  2:538473‑M1/249‑39 (MQ=255)                                                                                                                                                       ttAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAACCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCCACAAACTGAAAAAACGGGAGCCTGACGCacccgtcctctggtaacaaattaccaaccctgtcaaacc                                                                                         >  2:43437‑M1/1‑212 (MQ=255)                                                                                                                                                         aaCGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatcca                                                                                        <  1:496‑M1/250‑41 (MQ=255)                                                                                                                                                                cGAAAAGCCCCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGGGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaata                                                                                <  2:111796‑M1/251‑49 (MQ=255)                                                                                                                                                                 gAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatat                                                                               <  2:33190‑M1/251‑50 (MQ=255)                                                                                                                                                                   aaaGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatcaccaagaatat                                                                               >  2:469718‑M1/1‑200 (MQ=255)                                                                                                                                                                        ccctTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCTTGAAGAgcaggtcctcaggtaaaaaattaccaaccctgtcaatccacaagaatatagattct                                                                        <  2:425496‑M1/250‑57 (MQ=255)                                                                                                                                                                                    cgggttgACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtt                                                            <  2:29270‑M1/248‑69 (MQ=255)                                                                                                                                                                                    cgcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtt                                                            <  1:78882‑M1/251‑69 (MQ=255)                                                                                                                                                                                    cgcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtt                                                            <  1:478521‑M1/251‑69 (MQ=255)                                                                                                                                                                                     gcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCGGGGCAGAAGAGAGCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctgggaaaaaattaccatcccggtcaatccacaagaatatagatactgcaaagacggttt                                                           <  2:118043‑M1/251‑70 (MQ=255)                                                                                                                                                                                       gTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGaa                                                                                                                                  >  2:49329/1‑178 (MQ=255)                                                                                                                                                                                       gTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGaa                                                                                                                                  <  1:49329/178‑1 (MQ=255)                                                                                                                                                                                           aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacat                                                     <  1:47490‑M1/251‑76 (MQ=255)                                                                                                                                                                                                   catAGCCGCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcat                                             <  2:212572‑M1/251‑84 (MQ=255)                                                                                                                                                                                                      aGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatt                                          <  1:43437‑M1/251‑87 (MQ=255)                                                                                                                                                                                                      aGCCGCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatat                                           <  2:488207‑M1/250‑86 (MQ=255)                                                                                                                                                                                                                 agtcaatATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGTCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTTAAGAgcacgtcctctgttaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacgttttaaacatgtgttcatatttaaacatgtgc                               <  2:43422‑M1/248‑98 (MQ=255)                                                                                                                                                                                                                  gggaatatCTGTCAGGATGCGTCTCGCTGGATCATGCAAGCCGACCGTTTCGACGGCATTCCTCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAATCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcgtctggtaaaaaattaccaaccctgtcaatctacaagaatatagatactgcaaaggcggtttaatcatgtgttcatatttaaacatgtgct                              <  2:322898‑M1/248‑99 (MQ=255)                                                                                                                                                                                                                            gTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgc                    <  1:150939‑M1/251‑109 (MQ=255)                                                                                                                                                                                                                              cATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcat                  <  1:412210‑M1/251‑111 (MQ=255)                                                                                                                                                                                                                                        tCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgt         <  2:83671‑M1/250‑120 (MQ=255)                                                                                                                                                                                                                                        gccgCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgt         <  1:387579‑M1/249‑120 (MQ=255)                                                                                                                                                                                                                                         ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgta        <  1:405456‑M1/250‑121 (MQ=255)                                                                                                                                                                                                                                           gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCATCGAAACCATCAACCCGGATATCTGGGAAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatacacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgtaa       >  2:191050‑M1/1‑128 (MQ=255)                                                                                                                                                                                                                                              ggATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgtaaagta   <  1:232656‑M1/250‑126 (MQ=255)                                                                                                                                                                                                                                               gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATTAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGACAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatacacaagaatatagatacggcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgtaaagaaa  >  1:171903‑M1/1‑124 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGC  >  NC_000913/2251339‑2251826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑