Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	199.1 / NA	61	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/33);  total (28/33)

AAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGT  >  NC_000913/1337153‑1337632                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                aaGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                                                                                                                                                                         >  1:437761/1‑250 (MQ=255)  aGTGTGCTGATGGCCGCAGGCTTGCTGGCGANAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                                                                                                                                                                       <  2:247665/251‑1 (MQ=255)  aGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTCGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                                                                                                                                                                       <  1:299899/251‑1 (MQ=255)               ccGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcggtg                                                                                                                                                                                                                          <  1:416358/251‑1 (MQ=255)                   aGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGt                                                                                                                                                                                                                      >  1:408581/1‑251 (MQ=255)                            ggCGAAAAAAGCCGTAACTCTGGGCCTCAAGNGCCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                                                                                                                                                                             <  2:233744/251‑1 (MQ=255)                                    aaaccGTACCTCTGGGACGCAAGAGGCAACCATGGGTCAACGCTTCTCTGGCACCGGGTTCGAAAGTCGTTTATGATTATCTTGAAAAAGCGAAACTGACTCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATTCGGTTTTACCACCTGTAGTGGTAACTCTGGGCCGCTGCCCGCTCCTATCTAAACGGCAATCAAAAAAGGCGATTTAATCGCCGGTGCGGTGCTGTACGGCAACCGTAAC‑ttt                                                                                                                                                                                                    <  2:118367/248‑1 (MQ=255)                                       ccGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGaa                                                                                                                                                                                                 <  1:322127/251‑1 (MQ=255)                                         gTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                                                                                                                                                                             <  1:85942/238‑1 (MQ=255)                                         gTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                                                                                                                                                                             >  2:85942/1‑238 (MQ=255)                                                tGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTatc                                                                                                                                                                                        >  1:469733/1‑251 (MQ=255)                                                    ccTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTatccatc                                                                                                                                                                                    >  1:501343/1‑251 (MQ=255)                                                           cGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGACACGGCAATCAAAAAAGGCGATTTAACCGTCGGAGCGGTGCTGTCCGGCAACCG‑AACATTTGAAGGCAGTATCCATCCGCTGGt                                                                                                                                                                             >  2:105625/1‑251 (MQ=255)                                                            ggCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATGCGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGtt                                                                                                                                                                            <  2:382498/251‑1 (MQ=255)                                                               aaCCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTat                                                                                                                                                                                         >  1:107961/1‑235 (MQ=255)                                                               aaCCATGGGTACAAGCGTCGCGGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTat                                                                                                                                                                                         <  2:107961/235‑1 (MQ=255)                                                                   aTGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAaact                                                                                                                                                                      >  1:480431/1‑250 (MQ=255)                                                                           aaGCGTCGCTGGCCCCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGAATCTCGACGAACTGGGGTTTAACCTTGAGGGATACGGTTGTACCACCTGAATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGACACGGCAATCAAAAAAGGCGATTTAACCGTCTGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCAGCTGGTTAAAACTAActggctg                                                                                                                                                             >  2:387028/1‑251 (MQ=255)                                                                            aGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACGCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctgg                                                                                                                                                            <  2:50375/251‑1 (MQ=255)                                                                                   cTGGCACCGGGTTCGAAAGTCGTTTCTGATCATCTGGCCACAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGCTTTAACCGTCGGTGCGGTGCTGCCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgcc                                                                                                                                                     >  2:475847/1‑251 (MQ=255)                                                                                         ccGGGTTCGAATGTCGTTTCTGATTATCTGGCAAAGGCGAAACTGACCCCTTTTCCCGACGAACTGGGGTTTACCCTTGTGGGATACGGTTTTCCCACCGTTATTGGTAACTTGGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCACCCTTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTAAAACCTAACTGGCTGGCCTCGCCGCCGCt                                                                                                                                               <  2:516178/251‑1 (MQ=255)                                                                                           gggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtgg                                                                                                                                             >  1:442360/1‑251 (MQ=255)                                                                                            ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggt                                                                                                                                            <  1:46931/251‑1 (MQ=255)                                                                                                   aaGTCGTTTCTGATTATCTGGCAAAAGCGAANCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGcc                                                                                                                                     <  2:257156/251‑1 (MQ=255)                                                                                                              gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCt                                                                                                                             <  1:377191/248‑1 (MQ=255)                                                                                                              gATTATCTGGCAAAAGCGAAACGGACATCGTATCGCGACGAACGGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCGCGCCGCCGCTGGTGGTTGCCTATGCGCt                                                                                                                             <  2:377124/248‑1 (MQ=255)                                                                                                               aTTATCTGTCAAAAGCCAAACTTACACCGGATCGCGGCTAACTGGGTTTTAACCGTGTGGGATACGGTTGTACCTCCTGTATTGGTAACTCGGGGCCGTTGTCCTATCCTATCGTAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGGATGCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTGTGCGTTGGCg                                                                                                                         <  2:474182/251‑1 (MQ=255)                                                                                                               aTTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCg                                                                                                                         <  1:125396/251‑1 (MQ=255)                                                                                                                      ggCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAat                                                                                                                   >  1:196295/1‑250 (MQ=255)                                                                                                                              cGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGGAATATGAATATc                                                                                                          >  1:57631/1‑251 (MQ=255)                                                                                                                               gAAACTGACACCGTATCTCGACGAACTGGGGTTTCACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTCTCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCCCCGCTGGTGGTTGCCTATGCGCTGGCGGGACATATTAATATc                                                                                                          >  2:412617/1‑250 (MQ=255)                                                                                                                                 aaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAc                                                                                                       <  1:265983/251‑1 (MQ=255)                                                                                                                                     gACACCGTATCTCGACGAACGGGGGTTTAACCTTGTGGGATACGTTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTACCCGACGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTTGCCTCGCCGCCGCTGGTGGGTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTgg                                                                                                   <  1:411956/251‑1 (MQ=255)                                                                                                                                     gACACCGTATCCCAGCGAACTGGGGTTTAACCTTGTGGGATCCGGGTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGTCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAc                                                                                                       <  2:341999/247‑1 (MQ=255)                                                                                                                                          cGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCACGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGAGCGGTGCTGTCCGGCAACAGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGACTATCAACCTGGCTTCt                                                                                              >  1:149946/1‑251 (MQ=255)                                                                                                                                                 cgacgaTCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCt                                                                                        <  1:262985/250‑1 (MQ=255)                                                                                                                                                     gaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAAAATGAATATCAAACTGGCTTCTTAGCCTAACgg                                                                                   >  2:474802/1‑251 (MQ=255)                                                                                                                                                     gaACTGGGGTTTAACATTGTGGGATAAGGTTGTACCACCTGTATTGGTGACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTAGGGGCGGTGCTATCCGGCAACCGAAAA‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAACTCTGAATATAAACCATGCATCTGAGCCCATCgg                                                                                   >  2:423421/1‑251 (MQ=255)                                                                                                                                                            ggcttAACTTTTTGGGATACGGTTGGACCTCCTGTATTGGTAACTCTGGTCCGCTGCCCGATCCTATCTAAACGGCAATCAAAAAAGGCGATTTAGCCGCCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACGAACCGGCGGGTCTCGCCGCCGCGGGTGGTTGCCTGTGCGCTGGCTGGAGATATGCATATCAACCGGGCTTCTGAGCCTATCGGCTATGAt                                                                            <  2:480431/248‑1 (MQ=255)                                                                                                                                                             gTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                           <  1:132298/251‑1 (MQ=255)                                                                                                                                                                tatcgttGTGGGATGCGTTTGTTCCGCCTGGTTTGGTAACTCTGGGCCTCTGCCGGTGCCTATCGAAACGGCAACCAAAAAAGGCGATGTAACCGTCGGTGCGGCGCTGTCCGGCCACCGTAAC‑TTTGAGGGCCGTATCCTGCCGCTGGTTAAACCTAACGGGATGGCCTCGCCGCCGCTGGTGGTTGCCTCTGCGCTGGCGGGGAATATGAATATCAACCTGGCTTCTGAGCCTAGCGGCCATGATCGc                                                                         <  2:431851/245‑1 (MQ=255)                                                                                                                                                                 aaCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCCTCCGAGCCTATCGGCCATGATCGcc                                                                        >  1:247239/1‑249 (MQ=255)                                                                                                                                                                   ccTTGTGGGAAGAGGTTGTACCGCCTGGATTGGTATTACTGGGCCGCTGCCGGATCCTAGCGAAACGGCAATAAAAAAAGGCGATTTATCCGTCGCTGCGGTGCTGTCCGGCAACCGAAAC‑TTGGAAGGCCGTAGCCATCCGATGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCGAGGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTAAGCCTATCGGCCATGATCGCAAAg                                                                     <  2:52654/251‑1 (MQ=255)                                                                                                                                                                      tgggggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCTGCGGCCCGAACCTATCGAACCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTTCTGGCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATAGTCCATGATCGCAAAgg                                                                    <  2:30504/246‑1 (MQ=255)                                                                                                                                                                      tggggaatacggttttccgtcgtGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAGCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCGTCTTAGCCTATCGGCCATGATCGCAAAgg                                                                    <  2:300445/227‑1 (MQ=255)                                                                                                                                                                        tgGGATAAGGTTGTACCACCTGTATTGGTAAATCTGGGCCGATGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGCTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATACGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCCACCTGGCCTCTGAACCTCACGGGCATGACCGCAAAGGCGAt                                                                >  1:150752/1‑251 (MQ=255)                                                                                                                                                                          ggATGCGGGTGTACCACCTGTATGGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGGGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAATGCGATcc                                                              <  2:469733/251‑1 (MQ=255)                                                                                                                                                                          ggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCCTGAATGCAAAGGCGATcc                                                              >  2:34672/1‑251 (MQ=255)                                                                                                                                                                              aCGGTTGTACCCCCTTTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGt                                                           <  2:408581/250‑1 (MQ=255)                                                                                                                                                                                   tGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGACACGACAATCAAAAAAGGCGATTTAACAGTCGGTGCGGTGCTGTCCGGCCACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAAATGGCTGGCCTCGACGCCGCTGGTGATTGCCTATGCGCCGGCGGGCAATATCATTAACAACCTGGCTTCTGAGACAATCGGCCCTGATCGCAAAGGCGAtgcagattatat                                                     >  1:26831/1‑240 (MQ=255)                                                                                                                                                                                    gTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATTCGGTTTATCTg                                                    >  1:47615/1‑251 (MQ=255)                                                                                                                                                                                      accaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCACGATCGCACAGGCGATCCGGGTTTTCTgc                                                   >  2:494596/1‑249 (MQ=255)                                                                                                                                                                                                   gtaaagttGGGCTGCTGGCCGCTCCTATGGAAACGGTAATCAAAAAAGCCGATTTAACCGTCGGGGCGGTGCTGTCCGGCAACCGTCAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCCCGCCGCCGCTGGTGGTTGCTTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGATCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTTGCCa                                     <  2:318287/244‑1 (MQ=255)                                                                                                                                                                                                           gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                              >  1:396352/1‑250 (MQ=255)                                                                                                                                                                                                                gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCgg                                 >  1:47888/1‑242 (MQ=255)                                                                                                                                                                                                                gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCgg                                 <  2:47888/242‑1 (MQ=255)                                                                                                                                                                                                                     ccgttCCTACCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGGCCGGCAACCGTACC‑TTTGAAGGCCGTATCCACCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGTAATTGcc                   <  2:412867/247‑1 (MQ=255)                                                                                                                                                                                                                       gATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCg                 >  1:240397/1‑251 (MQ=255)                                                                                                                                                                                                                           cTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCg             >  1:280834/1‑251 (MQ=255)                                                                                                                                                                                                                                   aCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCCGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAACCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCAAAAGAAATTGCCCGTGCGGGAGAAc      >  1:373827/1‑250 (MQ=255)                                                                                                                                                                                                                                      gCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGt  <  1:490906/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                AAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGT  >  NC_000913/1337153‑1337632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑