Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,919	G→T	Q246Y (CAG→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,919	0	G	T	100.0%	216.7 / NA	62	Q246Y (CAG→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/34);  total (28/34)

ATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATG  >  NC_000913/1301674‑1302150                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        aTTGATGATCACACATTAGAAGTCACCTTAANTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                                                                                                                                                                                                    <  2:251065/251‑1 (MQ=255) aTTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                                                                                                                                                                                                    >  1:224843/1‑251 (MQ=255)        atCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGCAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg                                                                                                                                                                                                                             >  1:265460/1‑251 (MQ=255)             acaTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                                                                                                                                                                                                        <  1:251287/251‑1 (MQ=255)                        cACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                                                                                                                                                                                              <  1:458092/250‑1 (MQ=255)                                gTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                                                                                                                                                                                                     <  1:7440/251‑1 (MQ=255)                                    aCCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCATCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCCATTGCTTCTGAAGTTACCGATGTCa                                                                                                                                                                                                 <  2:295044/251‑1 (MQ=255)                                       cGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGGGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc                                                                                                                                                                                              <  2:157249/251‑1 (MQ=255)                                                     ataAATTACTTGTTCACCCATCAACTTCACCGGTTCAAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCGATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggt                                                                                                                                                                                >  2:383438/1‑251 (MQ=255)                                                       aaaTTACTTTTGCACCCATCAACTTCACCGTTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGTACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCATCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTACCTATGGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg                                                                                                                                                                               <  2:433974/250‑1 (MQ=255)                                                       aaaTTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg                                                                                                                                                                               >  1:3476/1‑250 (MQ=255)                                                       aaaTTACTTGTTCACCCATCAACATCACCGGTGCCAAAAGCCGCTAGCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGa                                                                                                                                                                              <  2:432383/251‑1 (MQ=255)                                                        aaTTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa                                                                                                                                                                             <  1:54513/251‑1 (MQ=255)                                                              ttGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTACTATCGTCACCAACGGTGCCTATACCTTCAAAGATTGGGTCGTAAACGAACGAAACGTTCTTGAACGCAGCACGACCTACTGGAACAACGCGAAAACCGTTATTAACTATATAACCTCTTTGCCTATTGCTTCTGAAGTTACCGATGACAACCGCTACCGTAGTGGTGAATTCGa                                                                                                                                                                        >  2:371494/1‑250 (MQ=255)                                                                    aCCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCANCCGCTACCGTCGTGGTGAAATCGACATgccg                                                                                                                                                                 >  2:258690/1‑248 (MQ=255)                                                                       aaTCAACTTACCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTAATGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACAGATGTCAACCGCTACAGTAGTGGTGAAATAGAAATTACGAAt                                                                                                                                                              >  2:162997/2‑251 (MQ=255)                                                                              ttCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGCAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAACCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGGCATGACGAATCACAgg                                                                                                                                                        >  2:158226/1‑249 (MQ=255)                                                                                cACCGGTGCCAAAAGCCTCGATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTTCACCTTAAAAGATTGGTTCTTAAACGAACGTATCGTTCTTGAACGTAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTGCTGAAGTTACCTATGTCAACCGCTACCGTAGTGGTGAAATCTACATGACGAATAACAGCATg                                                                                                                                                     <  2:441528/251‑1 (MQ=255)                                                                                cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCAt                                                                                                                                                      <  1:527078/250‑1 (MQ=255)                                                                                cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGAATg                                                                                                                                                     >  2:153136/1‑251 (MQ=255)                                                                                 cccGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGc                                                                                                                                                    <  1:188725/250‑1 (MQ=255)                                                                                  ccGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGcc                                                                                                                                                   >  1:204253/1‑251 (MQ=255)                                                                                     gtgccaaaagcctgcatgtataaaTCCGGCGAAAAATGGACCCACCCTGGTGAGATCGGCCACTACGGTGCAGATTCGTTAAAAGATTGTGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGTCCTACTGGTACACCGCGAAATCCGTTATTAACTATGTAACTTATTTGCGTATTTCTGCTGAAGTTTCCGATGTGATCCGCTTCCTTAGTGGTGAAGGCGACAGGCCGAATAACAGCATGCCGAt                                                                                                                                                <  2:327387/230‑1 (MQ=255)                                                                                           aaaGCCGCTATCGAGAAATTCGGCGAAAAATNGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATTGACATGACGAATAACAGCATGCCGATCGAAtt                                                                                                                                          <  2:271845/251‑1 (MQ=255)                                                                                           aaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAAtt                                                                                                                                          >  1:79758/1‑251 (MQ=255)                                                                                           aaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAAtt                                                                                                                                          >  1:179818/1‑251 (MQ=255)                                                                                            aaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTg                                                                                                                                         >  1:93122/1‑251 (MQ=255)                                                                                             aGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGt                                                                                                                                        >  1:376021/1‑251 (MQ=255)                                                                                                 gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTACACTATTTGCCTATTGCTTCTGAAGTTACCGATGACAACCGCTACCGTAGTGGAGAAATCGAAATGACGAATAACAGCATGCAGCTAGAATTGTTcc                                                                                                                                     >  2:290404/1‑250 (MQ=255)                                                                                                     gcGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTGCCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAg                                                                                                                                <  2:404102/250‑1 (MQ=255)                                                                                                          aaaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGa                                                                                                                            >  1:438231/1‑250 (MQ=255)                                                                                                           aaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaa                                                                                                                          <  1:516931/251‑1 (MQ=255)                                                                                                           aaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaa                                                                                                                           >  1:129039/1‑250 (MQ=255)                                                                                                               cGGCGAAAAATTGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCGTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATAACGAATAACAGCATTCCGAGCGAATTGTTCCAGAAGCTGaaaaaa                                                                                                                       <  2:489584/250‑1 (MQ=255)                                                                                                                                 gCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTc                                                                                                    <  1:431654/251‑1 (MQ=255)                                                                                                                                     ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGGCGTAAACGAACGAATCGTGCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCGATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                 <  2:188611/250‑1 (MQ=255)                                                                                                                                      gTAATATCGTCACCAACGGTGCCTATGCCTTAAAAGATTGGGTCGTAAACGGACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACATCGCGAAAACCGTGATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATTCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCActtt                                                                                               <  1:297366/251‑4 (MQ=255)                                                                                                                                      gTAATATCGGCACCAACGGTACCGATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCGATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTCCCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                               <  1:297378/251‑1 (MQ=255)                                                                                                                                                cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATa                                                                                       >  2:345653/1‑249 (MQ=255)                                                                                                                                                cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATa                                                                                       <  1:345653/249‑1 (MQ=255)                                                                                                                                                cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                     >  1:158663/1‑251 (MQ=255)                                                                                                                                                 aCCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCt                                                                                    <  1:33220/251‑1 (MQ=255)                                                                                                                                                      cGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc                                                                                >  1:270582/1‑250 (MQ=255)                                                                                                                                                          gCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTATGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGACGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACtt                                                                            >  1:313652/1‑250 (MQ=255)                                                                                                                                                                    tacaaGATTGCGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGCCCTACTGGAACAAAGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGACATCGACATGCCGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATACCGGACGAAGTTCACGTTAATCCATCCCTGAGCACATACTAttcaca                                                                  >  2:482889/4‑246 (MQ=255)                                                                                                                                                                       aaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAtt                                                              <  1:459011/251‑1 (MQ=255)                                                                                                                                                                            tGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc                                                                                                                                                                          <  1:355169/138‑1 (MQ=255)                                                                                                                                                                            tGGGTCGTAAACGAAAGAATCGTTCTTGAAAGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc                                                                                                                                                                          >  2:355169/1‑138 (MQ=255)                                                                                                                                                                                    aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAAGAACAGCATGCCGAGCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAGTAACAACCAGAAAc                                                 <  1:75941/251‑1 (MQ=255)                                                                                                                                                                                           cgaaTCGTTCTTGAAAGCAGCCCGACCTACTGGACCAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCAtt                                          >  2:516642/1‑251 (MQ=255)                                                                                                                                                                                            gaaTCGTTCTTAAACGCAGCCCGACCTACTGNAAAAACGAGAAAACCTTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGGGAAAACGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCATACCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTc                                         <  2:265026/251‑1 (MQ=255)                                                                                                                                                                                             aaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAAGAAATTAACAACCAGAAACCGCCATTc                                         >  1:199920/1‑250 (MQ=255)                                                                                                                                                                                               gctttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCTGTATTAACTTTGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGAAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATAACGAAATTAACAACCAGAAACCGCCATTCAAc                                      <  2:202475/248‑1 (MQ=255)                                                                                                                                                                                                      tgagcgctgCCCGACCTACGGGAACACCGCGAAAACCGTTATTACCTATGTAACCGATTTGCCTATTGCTTTTGAAGTTACTTTTGTCAACCGCTACCGTAGTGTTGAAATCGACATGACGAATACCAGCATGCCGATCGAATTGTTCCGGAAGCTGAAAAAAGAGATCCCGGACGAGTTTCCCGTGGGTCCATACCTGTGCACTTACTATTACGAAATTACCAACCAGAAACCGCCATTCAACGAtgt                                 <  1:347041/241‑1 (MQ=255)                                                                                                                                                                                                            cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGAtgtgcgtgtgc                         <  1:243944/251‑1 (MQ=255)                                                                                                                                                                                                                ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGNAACCGCCATTCAACGATGTGCGTGGGCGTAc                     >  2:271300/1‑251 (MQ=255)                                                                                                                                                                                                                    ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTGGCCTATTGCTTCTGAAGTTCCCGATGTCACCCGCTACCGTAGTGGTGAAATCGACATGACGAAAAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACGATGACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCa                 <  2:129039/251‑1 (MQ=255)                                                                                                                                                                                                                           gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtg                                                                                   <  2:255098/178‑1 (MQ=255)                                                                                                                                                                                                                           gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtg                                                                                   >  1:255098/1‑178 (MQ=255)                                                                                                                                                                                                                            aacaacGCGAAAACCGTTTTTATCTATGAACCCTATTTGCCTATTATTTATGAATTTACCGATGTCAACCGCTACCGTTGTGGTGAAACCGACATGACGAACAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGAACGAAGTTCCCGTTGATCCAACCCTGTGCACTTATTATTACGAATTAAACAGCCATAAACCGCCATTCAACGGTGTGCGTGTGCGTACCGTCCTGAAACt         <  2:443667/251‑1 (MQ=255)                                                                                                                                                                                                                              caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt         >  1:289821/1‑249 (MQ=255)                                                                                                                                                                                                                                   cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATGGCTTCTGAAGTTACCGATGTCACCCGCGACCGTAGTGGTGAAACCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCGGAAAAAAGAGAACCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATAACGAAATGAACACCCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATg  <  1:258690/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        ATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATG  >  NC_000913/1301674‑1302150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑