Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	100.0%	155.9 / NA	50	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/20);  total (30/20)

TACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCC  >  NC_000913/674289‑674763                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       tACGTTCTGGTCTTGCGGGCACCATTTGACCGCAGAAGTTTTCTTATATCCCAGGCCTTTTTTATACTGCTCGGGGAAGAATTTCTGTCCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGGGCCGGAGCGGTGTTGTTTTTCTCCGCCACGCCTTCCGCAGGCAGACCa                                                                                                                                                                                                                                    <  1:385841/250‑1 (MQ=255)                     cccAGTGGACCGCAGAAGTGTTCGTATATCCCAGTCCTTTTTAATCCGGCTCGTTGAAGATTTTCTGTTCCCAACGTTAGTATCCCGGCTTACAGTTTGCCAGCTCTGGGCTCCAGTCATACCCAAAGCCCTG‑CATTTGGAGCTGGTTTTTCT‑TCTCCGCGATGTTG‑TCGTACG‑CCACCGTTGCCGGAGCGTTGTTGTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAGCGGc                                                                                                                                                                                                               <  2:399713/250‑1 (MQ=255)                           tgcccGCAGAATTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGATTTTCTGTTCCCAACGGTAGTTTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCGGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATCCGCGATGTTG‑TCGTCCGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAg                                                                                                                                                                                                          <  1:18313/247‑1 (MQ=255)                            gACCGCAGACGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAAACCAAACGCGCCCCAGCCGATCGGCTGccggc                                                                                                                                                                                                        >  2:367336/1‑247 (MQ=255)                             cccGCAGAAGTCTTCTTATATACCAGGCTTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGAc                                                                                                                                                                                                       <  1:32445/250‑1 (MQ=255)                                             tatataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCAt                                                                                                                                                                                        >  1:91504/1‑250 (MQ=255)                                              atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATAc                                                                                                                                                                                      >  1:247010/1‑251 (MQ=255)                                               tataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTTCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAAACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATAcc                                                                                                                                                                                     >  1:265839/1‑250 (MQ=255)                                               tataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATAcc                                                                                                                                                                                     >  2:222372/1‑250 (MQ=255)                                               tataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGGTTTTGCCCAGCATACg                                                                                                                                                                                     >  1:118443/1‑251 (MQ=255)                                                 taCCAGGCATTTTTTATACAGCTCGGTGCAGAATTTATGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTA‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGATTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCCTACGCt                                                                                                                                                                                   >  2:460292/1‑251 (MQ=255)                                                            ttttaaacgccTCGGTAAGGAATTTCTGTCCCCAAGGGTTGTATTCCGGGGTAGAGGTTGCCGCCTCGGGGCCCCATCCATA‑CCAAAACCCCGACATTGTGGGCGGGTTTTTAAGTAT‑CGCGATGTTG‑CCGTCCGCCCCC‑GGTGCCGGGGCGGTGTTTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGGCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGc                                                                                                                                                                         <  2:109360/240‑1 (MQ=255)                                                              ttATACAGCTCGGTGAAGAATTTCTGTTCCCACCGGTAGTATTCCGGCGTACAGGGTGCCAGCTCGCGGCTCCAGTCATAACCACAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCCGCATACGCTGGTAGCGGGCgct                                                                                                                                                                      >  2:153369/1‑249 (MQ=255)                                                              ttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                                                                                                                      >  1:412318/1‑251 (MQ=255)                                                               ttaacagcctgttgagtatgttCTGCTCCCACCGGTAGTATTCCGGCGGACGGGTTGCCGCCTGGCGGCTCAAGTCATAACCAATGCCCGT‑GATTTTGAGGTGTTTTTGCA‑TATACGCGAGGTTG‑TCGTACTTCCCC‑GGGGCCGGCGCGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCCGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                                                                                                                      <  2:355975/230‑1 (MQ=255)                                                               tataCAGCTCGGTGAAGAATTTCTGTCCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGCCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                                                                                                                      <  1:241070/250‑1 (MQ=255)                                                                        gggTGAAGAATTTTTGTCCCAAAGGGTATTATTCCGGCGTACAGGTGGCCACCTCGCGGCTCAGGATAAAACAAAACCCCAA‑CTTTTTGAGCTGGTTTTTCA‑TATCCGCGTTGTTG‑CCGTCCGTCCCG‑GGGGCCGGGGCGGTGTTTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCg                                                                                                                                                           <  2:94638/250‑1 (MQ=255)                                                                         gttGAAGATTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCCCGGGGCTCCAGTCATACCCAAACCCCAG‑CATTTTGAGCTGTTTTTTCA‑TATCCGCGATGTTG‑TCGTCCGCCCGC‑GGGGCCGGAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGa                                                                                                                                                          <  1:347835/249‑1 (MQ=255)                                                                             aagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGc                                                                                                                                                                                                               >  1:418618/1‑195 (MQ=255)                                                                                aaTTTCTGTTCCCAACGGAAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGGCACCGATGgtgt                                                                                                                                                    >  2:141406/1‑250 (MQ=255)                                                                                   ttCTGATCCCAACGGAAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTACCCGATGGTGTCGtt                                                                                                                                                >  2:386020/1‑251 (MQ=255)                                                                                                    gtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCCGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCAACG‑CCCCGCTGGGGTAGTTACGTACGGGGCCCAtgg                                                                                                                               >  2:329498/1‑250 (MQ=255)                                                                                                    gtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCCGCATACGCTGGTAGCGGGCGATCA‑CGCCACCGCTGGTGTAGTGACGTACGTGGCCCAtgt                                                                                                                               >  2:522578/1‑251 (MQ=255)                                                                                                        cccGGCTTACAGGTTGCCAGCGCGCGGCTCCATTCATACCCAAAGCCAAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGAGGTTG‑CCGCACGTCCGC‑GGGCCCGGGGGGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAg                                                                                                                           <  1:81948/250‑1 (MQ=255)                                                                                                            gCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                                                                                                                        >  2:431799/1‑250 (MQ=255)                                                                                                                   ggTTGCCAGCTCGCGGCTCCAATCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCAAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGAACAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCCGCATACGCTGGTAGCGGGCGATCA‑CGTCCCCGCTGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGa                                                                                                                  >  1:142091/1‑249 (MQ=255)                                                                                                                    gTTGCCAGCTCGGGGCCCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCGGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGGGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAgg                                                                                                               <  1:460292/251‑1 (MQ=255)                                                                                                                           ccTCGCGGCCCCAGTCATAACCAAAGCCCGG‑CATTTTGAGCTGGTTTTTCA‑TATCCGCGGTGTTG‑TCGTCCGCCCCC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGa                                                                                                        <  1:367336/250‑1 (MQ=255)                                                                                                                               gcgGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGc                                                                                                     >  1:129921/1‑250 (MQ=255)                                                                                                                                cgGCCCCAGTCATAACCAAAGCCCAG‑CATTTTGCGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGACCGGCTGCAGGACGTTTTTGCCCATCATACGCTGGTAGCGAGCGGTCA‑CGTCACCGCGGGTGAAGTTACGGACGGGGCCCCTGTGTAGGCGACCAGAAGGATTGGGAAGCAt                                                                                                   >  2:288099/1‑251 (MQ=255)                                                                                                                                          cATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGAGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGGTAGGGAGGCATAGACAGGc                                                                                           >  2:405503/1‑249 (MQ=255)                                                                                                                                                catatgccgagaaTTTTGGGTTGGTTTTGCA‑TATCCTTGATGTTG‑TCGTGCGCGCCC‑GGTCGCGGAGCTGTTTTGTTTTCCCCCGTCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATa                                                                                    <  1:99458/239‑1 (MQ=255)                                                                                                                                                                    ggtgtgtttCA‑TATCCGCG‑TGTTGGTCGTACGCCCAC‑GTCGCCGGAGGGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCtt                                                                <  2:113991/245‑1 (MQ=255)                                                                                                                                                                                   cgtctaTGTTG‑TCTTCCGTCCAC‑GGTGCCGGAGCGGGGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCaaa                                                   <  1:285092/245‑1 (MQ=255)                                                                                                                                                                                       gATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgc                                              >  2:199345/1‑251 (MQ=255)                                                                                                                                                                                             g‑tCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑c                                                                                                                                                                  >  1:85754/1‑129 (MQ=255)                                                                                                                                                                                                  tACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCtt                                                                >  1:367340/1‑223 (MQ=255)                                                                                                                                                                                                         aC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTa                                                                                       >  1:285692/1‑193 (MQ=255)                                                                                                                                                                                                         aC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATg                              >  2:533801/1‑250 (MQ=255)                                                                                                                                                                                                            ggTGCCGGAGCGGTGTTGTTTTTCACCGCAACGCCTTCCGCCGGCAGCCCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCTGGCGATCA‑CGTCACCGATGGTGTACTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGGCTTCCGTTACTTCAATTGTGCGCCTCTCATccaaaggaa                            >  1:478242/1‑243 (MQ=255)                                                                                                                                                                                                                ccGGGGCGGTTTTGTTTTTCCCCGCCACGCCNCCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGt                       <  2:247010/251‑1 (MQ=255)                                                                                                                                                                                                                 gggcgCGGTGTTGTTTTTCCCCGCCACGCCTNCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCATGACGTTTTTGCCCAGGATACGCTGGTAGCGGGCGAGCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTa                      <  2:265839/247‑1 (MQ=255)                                                                                                                                                                                                                 cgtggCGGTTTTTTTTTTCCCCGCCACGCCTTCCGCAGTCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTTTCGTCTTCGGTTACTTCAAATGTTCGCTTCTCATCCCAATGAAGCTGTa                      <  1:298290/247‑1 (MQ=255)                                                                                                                                                                                                                   gAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACt                    >  1:79171/1‑251 (MQ=255)                                                                                                                                                                                                                   aaGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCGTGGGTAGTCGACAAGAAGGAGAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTGCGATAACTTCAAATGGGTGCTTCCCATCCCCATAAAGcgggcct                    >  2:186558/2‑245 (MQ=255)                                                                                                                                                                                                                       gtttttgttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTgg                <  2:109870/247‑1 (MQ=255)                                                                                                                                                                                                                       ggTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGaa                                                                                                                 >  1:385096/1‑154 (MQ=255)                                                                                                                                                                                                                               ttttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGCAGCTGTACTTTGGATTCTAtc        >  2:57820/1‑251 (MQ=255)                                                                                                                                                                                                                               ttttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGGCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGTTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAt         <  1:534082/250‑1 (MQ=255)                                                                                                                                                                                                                                 ttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtctc      >  1:159601/1‑251 (MQ=255)                                                                                                                                                                                                                                     ccgccaCGCCTTCCGCAGTCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTcc  <  1:540280/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTcc  <  1:153369/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       TACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAG‑CATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCAC‑GGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCA‑CGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCC  >  NC_000913/674289‑674763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑