Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,027	T→G	intergenic (‑36/‑323)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,027	0	T	G	100.0%	143.3 / NA	41	intergenic (‑36/‑323)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/24);  total (17/24)

GATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATC  >  NC_000913/4312789‑4313273                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       gATACCTTAACTATCATCGCTTCAGATACCGCCATAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAGCCACTCCACGATGCGGCTTTCATTTCCTGTCATTCCTTCCTGCTTCATTCGCAGGTACGGCGCTAACCCTATTCCGTTCGGAAGCGCTTAATCAAACGCTGACTGGCTTATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCTCCACAGc                                                                                                                                                                                                                                             <  1:537189/250‑1 (MQ=255) gATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGc                                                                                                                                                                                                                                             >  1:175906/1‑250 (MQ=255)                  cGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCt                                                                                                                                                                                                                           <  1:166933/251‑1 (MQ=255)                      tCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGc                                                                                                                                                                                                                        <  2:386220/250‑1 (MQ=255)                       cAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGGTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTTTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCATGAACAGAAAAGATGCTGGCGa                                                                                                                                                                                                                      <  2:398110/251‑1 (MQ=255)                            aCCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTc                                                                                                                                                                                                                  <  1:514557/250‑1 (MQ=255)                                ccAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTTAGTAACCACTCCACGATGCGGCTTTCATTGTCTGTCATTCCTGCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCGCGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTTGCGACTTCACaaa                                                                                                                                                                                                             <  1:316749/251‑1 (MQ=255)                                        tCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACATCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaataa                                                                                                                                                                                                      <  1:189663/250‑1 (MQ=255)                                                     tAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtt                                                                                                                                                                                         <  1:171530/250‑1 (MQ=255)                                                      aagtGCGGGTGCACTACTTAGGTTACCGTGTTTGAGTAACCACTCCACGATGGGGCTTTCATTTTCTGTCTTTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGTAAGCGCAGAATCAAACTCTGCCTGGCTCATCACGATCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGttt                                                                                                                                                                                        <  2:82769/247‑1 (MQ=255)                                                       aTTGCGGGTTCACAACTCAGGTTACCGTGTTTGAGTAACCACTCCACGATGCGGCTGTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATAACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttat                                                                                                                                                                                      <  2:294184/251‑1 (MQ=255)                                                            gggTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttattttat                                                                                                                                                                                 <  1:132326/251‑1 (MQ=255)                                                            gggTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttattttat                                                                                                                                                                                 <  1:147509/251‑1 (MQ=255)                                                                        cAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATgg                                                                                                                                                                     <  1:177487/251‑1 (MQ=255)                                                                         aggttaaagggTTTGAGTAACCACTCCACGATGCGGCTTTCAGTTTCTGTCATTCCTTCCTGCTTCATACGCAGGTAAGGCGCTAAACCTATACCGTTCGGACGCGCTGGATCACTCTCTGACTGGCTCATCACGCTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACACCAAGAACAGAAACGATGCAGGCGACTTATCCAAAACATAAAATTTAAAAAGTTTATTTTATTGTGAACAATGGc                                                                                                                                                                    >  2:169729/9‑251 (MQ=255)                                                                         aGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTTAACAATGGc                                                                                                                                                                    >  2:287668/1‑251 (MQ=255)                                                                             tACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGt                                                                                                                                                                >  1:21442/1‑251 (MQ=255)                                                                                 gggTTTGATTAACCACTCCACGATGCGGCTTTCATTTTCTTTCATTCCTTCCTGCTTCATGCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAAGCAAACTCTGACTGGCTCATCACGGTCTCTTGTCCTTGAAATGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATTCTGGCGAc                                                                                                                                                                                                                     <  2:430865/194‑1 (MQ=255)                                                                                 gggTTTGAGTAACCACTCCACGATGCGGCTTTCCTTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGCAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGCTCAACACAGCAAGAACAGAAAAGCTGCTGGCGAc                                                                                                                                                                                                                     >  1:430865/1‑194 (MQ=255)                                                                                     ttgattacccACTCCACGATGCGGCTGTCATTTTCGGTCATTCCTTCCTGCTGTATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTTAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGATGCCACTAGATAACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTAGGGTGAACAATGGCGAGTGGCTTaa                                                                                                                                                         <  2:429040/242‑1 (MQ=255)                                                                                           aaCCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtttt                                                                                                                                                   >  1:534882/1‑250 (MQ=255)                                                                                                gagggtgccgggcgcgccATTTTCTGACATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTATCCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCGGGCGACTTCACAAAAAAATAAAATATATAAAGTTTATTTGATTGTGAACAATGGCGAGTGGCTTAAAAGTTTATTTCa                                                                                                                                             <  2:142468/234‑1 (MQ=255)                                                                                                    aCGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAttt                                                                                                                                          >  1:183750/1‑250 (MQ=255)                                                                                                           ggCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGtt                                                                                                                                  >  1:144660/1‑251 (MQ=255)                                                                                                                  aTTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAAc                                                                                                                           <  1:83514/251‑1 (MQ=255)                                                                                                                         gTCAGTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTACTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATTGCGAGTGGCTTAAAATGTTATTTCATTTAATTAGTTTAAAAACatga                                                                                                                       <  2:464514/248‑1 (MQ=255)                                                                                                                            aTTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAt                                                                                                                 >  1:97678/1‑251 (MQ=255)                                                                                                                                    ctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGttttt                                                                                                         >  2:517528/1‑251 (MQ=255)                                                                                                                                     tgcttgagtagCAGGTAAAGCGCTAACCCTATACCGGTCGGAAGCGCTGAATCAAACTCTGACAGGCTCATCCCTTTCTCTTGTCATTGAATTAAAGACGCCACTAGATCACCACAGCAAGACCAGAAAAGATGCAGGCGACTTCACAAAAAAATACCATTTATAAAGTTTCTTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTGCATTAAATTAGGTGAAAACAAGGATGTTATTTGattttt                                                                                                        >  2:170527/11‑250 (MQ=255)                                                                                                                                             tCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttt                                                                                                 <  1:235790/250‑1 (MQ=255)                                                                                                                                              cGCAGGTTGGGCCCTAACCCTATGCCATTCGGAAGCGATGAATCGAACGCAGACTGGATCATCACGTTCTCATGTCCTTGAATTGAAGACGCCACTAGCTCAGCACAGCAAGAACATAAAAGATGCTGGCGACGTCACAAATAAATAAAATTTATAAAGTTTATTTTATTGTGAACAAGGGCGAGTCGCTTAAGATTTTATTTCAGTTAATTAGTTTAAAAACATGATGTTATTTGTATTTCTGGttttt                                                                                                <  2:534209/250‑1 (MQ=255)                                                                                                                                              cGCAGGTAAGGCGCTAACCCTATACCGATCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAAGTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGttttt                                                                                                <  2:289991/250‑1 (MQ=255)                                                                                                                                                cAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTTAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAATTTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATTATGTTATTTGTTTTTCTGGTTTTTTAg                                                                                             <  2:330954/251‑1 (MQ=255)                                                                                                                                                 aGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt                                                                                               >  1:142476/1‑248 (MQ=255)                                                                                                                                                    taatgcgcTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCCTCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGCCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGACGAGTGGCTTAAACTTTTATTTCATTTAATTAGTTTAAAAACAAGGTGTTATTTGTTTTTCTGGTTTTTTAgatgt                                                                                         >  2:162053/5‑250 (MQ=255)                                                                                                                                                            tAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGtt                                                                                 >  2:190320/1‑251 (MQ=255)                                                                                                                                                                           cGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAgt                                                                   <  1:326965/250‑1 (MQ=255)                                                                                                                                                                                  gcTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTa                                                            >  1:58965/1‑250 (MQ=255)                                                                                                                                                                                                     ggCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTc                                            >  1:149341/1‑247 (MQ=255)                                                                                                                                                                                                                       gTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAAc                         >  1:254195/1‑248 (MQ=255)                                                                                                                                                                                                                                           aCTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGATTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTTGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATGCATc  <  2:82081/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATC  >  NC_000913/4312789‑4313273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑