Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,289	A→G	Y235H (TAT→CAT)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,289	0	A	G	100.0%	132.1 / NA	39	Y235H (TAT→CAT)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/16);  total (23/16)

GTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGA  >  NC_000913/4312049‑4312504                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                        tgacgggaGGTAAGGGTACA‑GTAACTTCGCGATGGCAGAATTACCTTTTTCTGATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTGCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATAGGATCGGCCAGt                                                                                                                                                                                                               <  2:542805‑M2/242‑1 (MQ=255)                ggACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAAt                                                                                                                                                                                               <  1:281667/251‑1 (MQ=255)                         aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                                                                                                                                                        >  1:61366/1‑250 (MQ=255)                            ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                                                                                                                                                      <  1:204527/249‑1 (MQ=255)                                    gCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATAc                                                                                                                                                                             >  1:328981/1‑250 (MQ=255)                                          ttACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTAGCTATCGGTGAATGGTAGCTATGGGTTATACAaataat                                                                                                                                                                      >  1:124691/1‑251 (MQ=255)                                                     ttATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTCCGTTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGCCCCGt                                                                                                                                                           >  2:529293/1‑251 (MQ=255)                                                           aaGCGCGCCTGATGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGCTGCGCTGGGCGGCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTATTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATCTGATCGGCAAGTTTCGCTACCGGTGAATGGTAGATATGGGTTATACAAATAATCTTTGCCTAGTTCtttt                                                                                                                                                     >  2:506292/2‑251 (MQ=255)                                                            aGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCttttt                                                                                                                                                    >  1:162685/1‑251 (MQ=255)                                                              cgcgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTg                                                                                                                                                  >  2:401049/1‑251 (MQ=255)                                                                      gTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCt                                                                                                                                          <  1:215848/251‑1 (MQ=255)                                                                             gaaATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCg                                                                                                                                   <  2:1493/251‑1 (MQ=255)                                                                               aaTCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCt                                                                                                                                 <  1:371518/251‑1 (MQ=255)                                                                                      aaTTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACAt                                                                                                                          <  1:214227/251‑1 (MQ=255)                                                                                               ggTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTc                                                                                                                 <  1:74434/251‑1 (MQ=255)                                                                                                 gtttttGCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGGAATATTCTTGCCAAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGGGAATGGTAGCTATGGGTTATACCAATAATCTTTGACCCGTGCTTTTTTGCCNGTTTTAAGGCAGCTTTTACATCACTGGTTCg                                                                                                                >  2:254152/2‑251 (MQ=255)                                                                                                        c‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTTCATCACTGGTTCGCCCGGAAt                                                                                                        >  2:303857/1‑251 (MQ=255)                                                                                                                 tattaGCTTGTTCAATGTTGAGCTGGGCGACAGCGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATCTGATCGGCCCGGTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGACGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACt                                                                                                >  2:73605/1‑251 (MQ=255)                                                                                                                     aGCTTGTTCAATGGTGAGCTGGCCGACAGAGACAAAAAAAGCGTTCAGCTAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTACTGGGGAATGGAAGCTATGGGTTATACAAATAATCATTGCCCCGTTTTTTTTTTCCAGTTCTACGGCCACTTTTTCATCACTGGTTTGCCCGGAATGGGTCACttcc                                                                                             >  2:177487/1‑247 (MQ=255)                                                                                                                      gCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTAcc                                                                                             >  1:491105/1‑249 (MQ=255)                                                                                                                          gTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAAGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGcaca                                                                                       <  1:303857/251‑1 (MQ=255)                                                                                                                               aTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtc                                                                                  >  2:202018/1‑251 (MQ=255)                                                                                                                                  ttGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcc                                                                               >  1:529934/1‑251 (MQ=255)                                                                                                                                   tGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcct                                                                              >  1:471649/1‑251 (MQ=255)                                                                                                                                   ggAGCTGTGCGCCAGAGACAAAAAATATGCCCAGCAAGGCTGGTTTTAATATTCTTGCCTAGGCATGACGATCTAATAACGGCGTTTCCGGGGATTGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTGAGTCAGATAATCTTTGCCCCGTTCTTTGTTGCCAGTTCTTCGGCCTCATTTACATCTCTGGGTCGCCCGGAATGGGTCACGAACAGCACAACAtcttct                                                                              <  2:511362/250‑4 (MQ=255)                                                                                                                                    gAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcctt                                                                             >  1:232981/1‑251 (MQ=255)                                                                                                                                               ccgaaaCAAAAAAAGCGTCCTGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCTCTATCGGTGAATTGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTatcaac                                                                  <  2:378651/246‑1 (MQ=255)                                                                                                                                               cAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGATGGTGAGCAAATAATATGATCGGCCAGTTTAGCTATCGGTGCATGGTAGCTATGGGTTATACAGATAATCTTTGCCCCGTTCTTTTTTGCAAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGCATGGGTAANTACCAGCACAATATCTACTCCCTGTaacaac                                                                  >  2:235790/1‑251 (MQ=255)                                                                                                                                                     cAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCCCTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCg                                                            >  2:141041/1‑251 (MQ=255)                                                                                                                                                           aaGCGTCCAGCAAGGTTGGGGGTAATCTTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGTGCTGGTGAGCAAATGATATGATCGGCCACTTCCCCTATCGGCGAATGGTAGCTATGGGCTATACAAATAATTCTTGCCCCGTTCTTTTTTGCCTGATCTACGGGCGCTTTTACATCACTGGTTCGCCCGGCATGGGTCACTACAAGCACACCCTCAACCACGTGTAACAACGAAGCggccct                                                       >  2:342752/1‑246 (MQ=255)                                                                                                                                                                 ccAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGTCCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATAGGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTGGCCAGTTCTACGGCCGCTTTTACATCTCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCatg                                                <  1:474078/251‑1 (MQ=255)                                                                                                                                                                                     atTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGc                            <  1:401049/251‑1 (MQ=255)                                                                                                                                                                                            ccGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAggccaggtcgcgtac                     >  2:114896/1‑238 (MQ=255)                                                                                                                                                                                                     tACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGcc             <  1:463859/250‑1 (MQ=255)                                                                                                                                                                                                     tACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGcc             <  2:177416/250‑1 (MQ=255)                                                                                                                                                                                                        gACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGCGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCCCTGGTTCGCCCGGAATGGGTCACTCCCAGCACAACATCTCTCTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCCACAGTATAGGCCTGACAGCGTACGCCAATg         >  2:381261/1‑251 (MQ=255)                                                                                                                                                                                                            taatacCGGCGTTTACGGGGCTGGTGGGCAAATAATATGATAGAAAAGTTTCGCTATCGGTGCATGGTAGCTATGGGTTATACACATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGACGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCCCAACATCTCCTTCCTTTAAAACCCAAGCGGTCATCATCATGATGTGCGCATCNGGATAAGCCTGACAGCGTACCCCAATggggc     >  2:237732/1‑247 (MQ=255)                                                                                                                                                                                                              ataaCGGCGGTTCCGGGGCTTGTGAGCAAATNAGATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAg   <  2:242439/251‑1 (MQ=255)                                                                                                                                                                                                                aaCGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGa  >  1:410059/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                        GTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGA  >  NC_000913/4312049‑4312504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑