Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,219	A→G	L258P (CTA→CCA)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,219	0	A	G	100.0%	138.4 / NA	40	L258P (CTA→CCA)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/16);  total (24/16)

GCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATG  >  NC_000913/4311981‑4312458                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                ggtcattccctgatattacgggcactatttattcaaatctctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATGTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCt                                                                                                                                                                                                                                    <  2:412948‑M2/175‑1 (MQ=255)             atattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCAtt                                                                                                                                                                                                                        <  1:114087‑M2/187‑1 (MQ=255)               attacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGCCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTAc                                                                                                                                                                                                                      >  1:242439‑M2/63‑251 (MQ=255)                              tattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGTGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGc                                                                                                                                                                                                        <  1:52288‑M2/203‑1 (MQ=255)                                 tcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGttt                                                                                                                                                                                                    >  1:35012‑M2/45‑251 (MQ=255)                                              gaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTg                                                                                                                                                                                        >  1:1493‑M2/32‑250 (MQ=255)                                                  aacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCa                                                                                                                                                                                    >  1:365666‑M2/28‑250 (MQ=255)                                                    caggctgtcgtcagttttgacgtgaGGTAAAGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAaat                                                                                                                                                                                 >  2:74434‑M2/26‑251 (MQ=255)                                                            cgtcagttttgactggaGGTAAGGGGACA‑TTTACTCCGCGATGGATGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTGCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCaaataatatga                                                                                                                                                                          <  2:316487‑M2/233‑3 (MQ=255)                                                                     tgacgggaGGTAAGGGTACA‑GTAACTTCGCGATGGCAGAATTACCTTTTTCTGATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTGCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATAGGATCGGCCAGt                                                                                                                                                                 <  2:542805‑M2/242‑1 (MQ=255)                                                                                    ggACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAAt                                                                                                                                                 <  1:281667/251‑1 (MQ=255)                                                                                             aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                                                                                                          >  1:61366/1‑250 (MQ=255)                                                                                                ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                                                                                                        <  1:204527/249‑1 (MQ=255)                                                                                                        gCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATAc                                                                                                                               >  1:328981/1‑250 (MQ=255)                                                                                                              ttACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTAGCTATCGGTGAATGGTAGCTATGGGTTATACAaataat                                                                                                                        >  1:124691/1‑251 (MQ=255)                                                                                                                         ttATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTCCGTTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGCCCCGt                                                                                                             >  2:529293/1‑251 (MQ=255)                                                                                                                               aaGCGCGCCTGATGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGCTGCGCTGGGCGGCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTATTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATCTGATCGGCAAGTTTCGCTACCGGTGAATGGTAGATATGGGTTATACAAATAATCTTTGCCTAGTTCtttt                                                                                                       >  2:506292/2‑251 (MQ=255)                                                                                                                                aGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCttttt                                                                                                      >  1:162685/1‑251 (MQ=255)                                                                                                                                  cgcgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTg                                                                                                    >  2:401049/1‑251 (MQ=255)                                                                                                                                          gTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCt                                                                                            <  1:215848/251‑1 (MQ=255)                                                                                                                                                 gaaATCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCg                                                                                     <  2:1493/251‑1 (MQ=255)                                                                                                                                                   aaTCAACAATTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCt                                                                                   <  1:371518/251‑1 (MQ=255)                                                                                                                                                          aaTTGCGCCGGTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACAt                                                                            <  1:214227/251‑1 (MQ=255)                                                                                                                                                                   ggTTTTTTCC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTc                                                                   <  1:74434/251‑1 (MQ=255)                                                                                                                                                                     gtttttGCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGGAATATTCTTGCCAAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGGGAATGGTAGCTATGGGTTATACCAATAATCTTTGACCCGTGCTTTTTTGCCNGTTTTAAGGCAGCTTTTACATCACTGGTTCg                                                                  >  2:254152/2‑251 (MQ=255)                                                                                                                                                                            c‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTTCATCACTGGTTCGCCCGGAAt                                                          >  2:303857/1‑251 (MQ=255)                                                                                                                                                                                     tattaGCTTGTTCAATGTTGAGCTGGGCGACAGCGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATCTGATCGGCCCGGTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGACGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACt                                                  >  2:73605/1‑251 (MQ=255)                                                                                                                                                                                         aGCTTGTTCAATGGTGAGCTGGCCGACAGAGACAAAAAAAGCGTTCAGCTAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTACTGGGGAATGGAAGCTATGGGTTATACAAATAATCATTGCCCCGTTTTTTTTTTCCAGTTCTACGGCCACTTTTTCATCACTGGTTTGCCCGGAATGGGTCACttcc                                               >  2:177487/1‑247 (MQ=255)                                                                                                                                                                                          gCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTAcc                                               >  1:491105/1‑249 (MQ=255)                                                                                                                                                                                              gTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAAGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGcaca                                         <  1:303857/251‑1 (MQ=255)                                                                                                                                                                                                   aTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtc                                    >  2:202018/1‑251 (MQ=255)                                                                                                                                                                                                      ttGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcc                                 >  1:529934/1‑251 (MQ=255)                                                                                                                                                                                                       tGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcct                                >  1:471649/1‑251 (MQ=255)                                                                                                                                                                                                       ggAGCTGTGCGCCAGAGACAAAAAATATGCCCAGCAAGGCTGGTTTTAATATTCTTGCCTAGGCATGACGATCTAATAACGGCGTTTCCGGGGATTGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTGAGTCAGATAATCTTTGCCCCGTTCTTTGTTGCCAGTTCTTCGGCCTCATTTACATCTCTGGGTCGCCCGGAATGGGTCACGAACAGCACAACAtcttct                                <  2:511362/250‑4 (MQ=255)                                                                                                                                                                                                        gAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtcctt                               >  1:232981/1‑251 (MQ=255)                                                                                                                                                                                                                   ccgaaaCAAAAAAAGCGTCCTGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCTCTATCGGTGAATTGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTatcaac                    <  2:378651/246‑1 (MQ=255)                                                                                                                                                                                                                   cAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGATGGTGAGCAAATAATATGATCGGCCAGTTTAGCTATCGGTGCATGGTAGCTATGGGTTATACAGATAATCTTTGCCCCGTTCTTTTTTGCAAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGCATGGGTAANTACCAGCACAATATCTACTCCCTGTaacaac                    >  2:235790/1‑251 (MQ=255)                                                                                                                                                                                                                         cAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCCCTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCg              >  2:141041/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGCGTCCAGCAAGGTTGGGGGTAATCTTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGTGCTGGTGAGCAAATGATATGATCGGCCACTTCCCCTATCGGCGAATGGTAGCTATGGGCTATACAAATAATTCTTGCCCCGTTCTTTTTTGCCTGATCTACGGGCGCTTTTACATCACTGGTTCGCCCGGCATGGGTCACTACAAGCACACCCTCAACCACGTGTAACAACGAAGCggccct         >  2:342752/1‑246 (MQ=255)                                                                                                                                                                                                                                     ccAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGTCCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATAGGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTGGCCAGTTCTACGGCCGCTTTTACATCTCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCatg  <  1:474078/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                GCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGC‑ATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATG  >  NC_000913/4311981‑4312458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑