Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,893	A→C	A219A (GCT→GCG)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,893	0	A	C	100.0%	224.9 / NA	68	A219A (GCT→GCG)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (40/28);  total (40/28)

AGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTAC  >  NC_000913/3815653‑3816138                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCcaaca                                                                                                                                                                                                                                                >  1:35436/1‑249 (MQ=255)            gaagaaATAGGTGCTTTTTCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACTCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATAGGGTGTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGct                                                                                                                                                                                                                                    <  2:446974/250‑1 (MQ=255)                    aGGGGCTTTTGCGCCCGGNTTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGt                                                                                                                                                                                                                                        <  2:238927/238‑1 (MQ=255)                    aGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGt                                                                                                                                                                                                                                        >  1:238927/1‑238 (MQ=255)                    aGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCCTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATgg                                                                                                                                                                                                                            <  1:183833/250‑1 (MQ=255)                       ggCTTTTGCGCCCGGATTTCAGCGTAAACACNCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCAGCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGa                                                                                                                                                                                                                        <  2:249792/251‑1 (MQ=255)                         cTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAACGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAAc                                                                                                                                                                                                                      >  1:472566/1‑251 (MQ=255)                           tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACg                                                                                                                                                                                                                     <  1:428267/250‑1 (MQ=255)                           tttGCGACCGGATTTCCGCGTAAACTCGCAAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGGGAACgg                                                                                                                                                                                                                    >  1:429917/1‑251 (MQ=255)                             ttcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCAC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGc                                                                                                                                                                                                                   <  2:35436/248‑1 (MQ=255)                             tGCGCCCGTATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCTCAAATTCAATAAGCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAGAGGCGGCTCATCAGGCGCATTAAAAAGCAGTTTGCCAGCGCCGCCTTGTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGGCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGc                                                                                                                                                                                                                   <  2:143015/250‑1 (MQ=255)                                              gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                                                                                                                                                 >  1:91726/1‑251 (MQ=255)                                              gTAAACCCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                                                                                                                                                 <  2:91726/251‑1 (MQ=255)                                                    ccgcgtaacgttaccacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt                                                                                                                                                                                           <  1:253298/237‑1 (MQ=255)                                                      gCCAAACTTTAACACCTGCTTGCTAAGCGCCAATTCAATAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAACAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACACGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc                                                                                                                                                                                         >  2:480647/1‑251 (MQ=255)                                                        cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGACTTAGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGTGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCa                                                                                                                                                                                        >  2:402158/1‑250 (MQ=255)                                                               tAACACCTGCTTGCTAAGCGCAAATTCAATTAACGGGCGCTGATATGGTTTCATGCCTTCGCTCCTCAACGTACTTTTCGACAGACAAAAAAAAGGCGACACATCAGTCGCCTTAAAAATCAGTTTTCCAGCGCCGCCTTCTGCGTCGCGCCAATGTATTCGAGTCC‑CCC‑TC‑GGGCCAGCGCCAACAAGGGGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTACCCTGCACTGCAATGATgc                                                                                                                                                                                 <  2:335057/250‑1 (MQ=255)                                                                aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                                                                                                                                               <  1:372187/251‑1 (MQ=255)                                                                           gcaacgcgaaaattcaagatccttgcgcTGAAATGTTTTCATGCCTTCGCTCCGCATCTTACTTTTCTACAGACAAAAAAAAGGCGACGCAGCAGTCTCCGTAAAAATCAGTTTGCCATCGCCGCCATCTGCTTCGCTACAATGGAGTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTATCTCTTCATGGGTGAACGGCTCGCCTTCTGTCGTCCCCTGCACTTCTATGATGCGCCCGTCTTCGGt                                                                                                                                                                    <  2:282294/227‑1 (MQ=255)                                                                                             aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGCCGCTAAAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATAACTACGTTCATGTc                                                                                                                                                  >  2:178271/1‑251 (MQ=255)                                                                                             aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCCCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                                                                                                                                                  <  1:276112/251‑1 (MQ=255)                                                                                                  ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct                                                                                                                                             >  1:336019/1‑251 (MQ=255)                                                                                                     gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGGCTTCGGTCATCACTACGTTCATGTCGGTCTCTg                                                                                                                                          >  1:480168/1‑251 (MQ=255)                                                                                                       tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg                                                                                                                                        >  1:267505/1‑251 (MQ=255)                                                                                                              gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACCCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGt                                                                                                                                 >  1:322144/1‑251 (MQ=255)                                                                                                                 tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCCTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGCGTCtt                                                                                                                              >  1:260646/1‑251 (MQ=255)                                                                                                                       cTTCGCTACTCATCTTACTTTTCTACAGACAACAAACAGGCGACTCATCAGTCGCCTTAACAATCAGTTTGCCAGAGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGACAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCCATGCTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCCGAGGCTTCAACgg                                                                                                                        >  2:206813/1‑250 (MQ=255)                                                                                                                          cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTAtt                                                                                                                     >  1:414893/1‑251 (MQ=255)                                                                                                                                 cATCTTACTTTTCTACAGACAAAAAAAAGNCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCCGCGCCGCCGTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACTTATTCCAGa                                                                                                                <  2:249094/249‑1 (MQ=255)                                                                                                                                          tttCTACAGACAAAAAAAAGGCGCCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaatt                                                                                                       <  1:117613/249‑3 (MQ=255)                                                                                                                                           ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaatttct                                                                                                    >  2:322001/1‑246 (MQ=255)                                                                                                                                             cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaattgcttc                                                                                                  >  2:511074/1‑244 (MQ=255)                                                                                                                                               aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaattgcttcac                                                                                                <  1:389571/251‑10 (MQ=255)                                                                                                                                               aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaattgcttc                                                                                                  <  1:471834/249‑8 (MQ=255)                                                                                                                                                  gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGCACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGt                                                                                             >  2:182970/1‑251 (MQ=255)                                                                                                                                                  gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGCCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTATTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGt                                                                                             >  1:155674/1‑251 (MQ=255)                                                                                                                                                     aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGGCTTCAACGTATTCCAGATCGCAAATTGCTTCCCCGttt                                                                                           >  2:422116/1‑249 (MQ=255)                                                                                                                                                      aaacaaaGGCGCCGCATCAGCCGCCTTAAAAATCAGTTGGCCAGCGCCGTCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCGC‑GGGCCAGCTCCAACAAGGTGAGTAGCTCTTCATGGGTTCACGGCTCGCCGTCTGCCATCCCCTGCACTGCAATTATGCGCCCGGCTTCGGTCATCACTCCGTGCATGTCGGTCTCTGCGGCTTATTCTTCAACGGATTCCAGATCGCAAAGTGCTTCACCGtttac                                                                                         <  2:210915/247‑4 (MQ=255)                                                                                                                                                          aaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTCCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCACCC‑TC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGCAATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAAt                                                                                      >  1:183388/1‑250 (MQ=255)                                                                                                                                                           aaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAAtac                                                                                    >  1:262752/1‑249 (MQ=255)                                                                                                                                                             ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCg                                                                                  <  1:440499/251‑1 (MQ=255)                                                                                                                                                             ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCg                                                                                  <  1:344055/251‑1 (MQ=255)                                                                                                                                                               cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                >  1:197973/1‑251 (MQ=255)                                                                                                                                                                gaatcataaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCATC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCCACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                >  1:293667/4‑250 (MQ=255)                                                                                                                                                                         gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCg                                                                      >  1:510088/1‑251 (MQ=255)                                                                                                                                                                           cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGGCTCTGCGGCTGAGTCTTCAACGTATTCCCGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGc                                                                    >  2:384908/1‑251 (MQ=255)                                                                                                                                                                           cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg                                                                                                                                        <  1:229719/183‑1 (MQ=255)                                                                                                                                                                           cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg                                                                                                                                        >  2:229719/1‑183 (MQ=255)                                                                                                                                                                               ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGAATCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGGCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCACATTGCTTCACCGTTTACAATACCGACAGAACCTGCGGCTaac                                                                >  2:303507/1‑249 (MQ=255)                                                                                                                                                                                 aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGCTTACAATACCGACAGACACTGCGGCTAccct                                                              >  2:142792/1‑249 (MQ=255)                                                                                                                                                                                   aaaTCAGTTTGCAAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCACAAAGGCGAGTAGCTCTTGATGGGTGAACGGCTCGCCTTCTTCCGTCCCCTGCCCTTTAATTGTGCCCCCATCGATGGTGTTCACTACCTTCATGTCGGTCTATTCTGCTGACTCTTCAATGTATCGCAGGTCCCAAATTGCTTCAGCGTTNACAATCCCGCCAGAAACTGCGGccaccaaac                                                            >  2:255360/1‑248 (MQ=255)                                                                                                                                                                                            gtcccgcgcCGCATTCTGCGTCGCTACAATGTATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCAAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCGGCTCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCTAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCAGCCCCTTCATCg                                                   <  2:488228/246‑1 (MQ=255)                                                                                                                                                                                             gCCAGCGCCGCCTTCTTCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGCCATCACTACGTTCATGTCGGCCTCTGCGGCTGAGGCTTCACCGTATTCCAGATCGCAACTTGCTTCCCCGTTTACACTACCTACAGAAACTGCGGCTACCACCCCTTTCCTCgg                                                  >  2:66177/1‑251 (MQ=255)                                                                                                                                                                                                aGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAAAGAAACTGCCGCCACCATCCCTTTCATCggggt                                               >  2:285705/1‑248 (MQ=255)                                                                                                                                                                                                aGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCgggtt                                               <  1:322001/251‑4 (MQ=255)                                                                                                                                                                                                    ccgccTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGCTGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAACCTGCGGCTACCCTCCCTTTTATCCggttggtt                                           >  2:37546/1‑251 (MQ=255)                                                                                                                                                                                                    ccgccTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCCTCGggttggtt                                           >  1:308149/1‑251 (MQ=255)                                                                                                                                                                                                           cTGCGTCGCTACAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCt                                    >  2:378524/1‑251 (MQ=255)                                                                                                                                                                                                                     acaagagctgcgatgcc‑ccc‑TC‑GGGCCAGCGCCAACATGGGTAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCTCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTTTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCTTCCCTTTCAACGGGTTGGTTTTCAGCTTGCCGGTTTc                          <  2:366404/235‑1 (MQ=255)                                                                                                                                                                                                                     aCAAGGGATTCGA‑TTC‑CCC‑CCCGGGCCAGCGCCAACCAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCGCTTCAATGAGGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCTGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATATCGACAGAAACTGCGGCTACAATCCCTTTCATCGGGTTGGTGTTCAGCTTGCCGtttt                           <  2:193/250‑1 (MQ=255)                                                                                                                                                                                                                      cAATGGATTCGA‑TTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAACCTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTTCCGTTTTcc                         >  2:19336/1‑251 (MQ=255)                                                                                                                                                                                                                              tcga‑ttc‑CCCCTC‑GGGCCAGCGCCAACAAGGTGCGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGACACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCCCCAGCtt                 >  2:64700/1‑251 (MQ=255)                                                                                                                                                                                                                                            c‑GGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCGTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGATCATCTCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGCCAGAAACTGCGTCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATc     <  1:10630/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCt                                    <  1:53690/218‑1 (MQ=255)                                                                                                                                                                                                                                               ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCt                                    >  2:53690/1‑218 (MQ=255)                                                                                                                                                                                                                                               ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtg   >  1:45310/1‑250 (MQ=255)                                                                                                                                                                                                                                                gCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCCATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtgc  >  1:314931/1‑249 (MQ=255)                                                                                                                                                                                                                                                gCCAGCGCCAAAAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCGGACTTCCCCTGCACTTCAATCATGCGCCCGTCTTCGGGCATCACTACGTTCATGTCGGTCTCGGCGGCTGAGTCTTCAACGGATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTGCCGCCAGCTTCTGTAGCGCATCtgc  <  2:440436/251‑3 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTAC  >  NC_000913/3815653‑3816138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑