Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,517	T→C	A84A (GCA→GCG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,517	0	T	C	100.0%	154.8 / NA	45	A84A (GCA→GCG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/38);  total (7/38)

GCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGG‑TGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGA  >  NC_000913/3815272‑3815757                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAACCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCcac                                                                                                                                                                                                                                              <  2:87614/251‑1 (MQ=255)           cgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCAGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtag                                                                                                                                                                                                                                      <  2:393086/249‑3 (MQ=255)                cgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCATGATGCTCCGCCAGCGCCAcggcgggagggggcgc                                                                                                                                                                                                                               >  1:92318/1‑241 (MQ=255)                      cgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCgg                                                                                                                                                                                                                        <  2:92318/251‑1 (MQ=255)                              aCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCtt                                                                                                                                                                                                                <  1:57513/251‑1 (MQ=255)                                    aaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAg                                                                                                                                                                                                          <  1:346883/250‑1 (MQ=255)                                        gAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAgg                                                                                                                                                                                                      <  1:206272/251‑1 (MQ=255)                                              aatacaccAGCAAGCGGCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCaaa                                                                                                                                                                                                <  2:86325/245‑1 (MQ=255)                                                     cAGCAAGCGTCCCGCCATTAGCCTGCATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCACCCGTATTTCCTCCTTCACCGTGGTCTTTTGCCTCTTTTCGGTTAAAGCAGTGCGGCAGGTCAACGTCATGATGCTTCGCCAGCGCCACGGCGGTAGTGT‑TCGCAATCGGTATCCCGTTGTAAGCAGGTCCAAAcagcagt                                                                                                                                                                                         <  1:426872/251‑2 (MQ=255)                                                       gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑ACGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATc                                                                                                                                                                                       <  1:37491/251‑1 (MQ=255)                                                            cGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACt                                                                                                                                                                                  <  1:147455/251‑1 (MQ=255)                                                              tCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGACTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATTATGCTCCGCCAGCGCCACGGCGGGAGTGGGTGGAAACTGGGA‑CCCTTTGTAAGCAGGGGCCAAcaggcaatggacctcc                                                                                                                                                                                >  1:210915/1‑238 (MQ=255)                                                                gcgcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGG‑TCGCCAACGGGGTCCCCTTTTAAGGAGGGCCAAAAAGGCGATCGAACACaa                                                                                                                                                                               >  1:249792/1‑250 (MQ=255)                                                                            tGAATAATCTCCATCGATTCGCGAATCGCCGTTCAGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGCCAAGGGCATGATGCCCCGCCAGCGCCACGGCGGGTGGGG‑GCGCCAACGGGAACCCCTTGTAAACAGGGCCAAACAGCCGATAGAAcccacaacccgaatcccc                                                                                                                                                                  >  1:143015/1‑234 (MQ=255)                                                                                 aatCTCCATCGATGCGCTAATCGCCGTTCCGGCGGTGATCACATCACCTACCATCAGTACGCGTCCTTGTAACGCTCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTTTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCTGTAGTGG‑TCGCAATCGGGATCCCTGTGGAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAAGCCCCCaatg                                                                                                                                                             <  2:174347/251‑3 (MQ=255)                                                                                                         cGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACa                                                                                                                                     <  1:234449/251‑1 (MQ=255)                                                                                                          gTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAg                                                                                                                                    <  1:16742/251‑1 (MQ=255)                                                                                                                 cggTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTCCCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAgg                                                                                                                             <  2:58531/251‑2 (MQ=255)                                                                                                                     gATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTcg                                                                                                                          <  1:485528/250‑1 (MQ=255)                                                                                                                                  aCCAGCATTACGCGTCCTTGGTACGCGCTTCCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACTGCCTAACAGTGCCAGGTCGCGCCCGGTAtt                                                                                                               <  2:352733/248‑1 (MQ=255)                                                                                                                                     aGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAg                                                                                                         <  1:118606/251‑1 (MQ=255)                                                                                                                                        aTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                                              <  2:88227/243‑1 (MQ=255)                                                                                                                                        aTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGG‑TCGCAATCGGGATCCCTTTTTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGCAAAAAAGGCCCAACAGTGCCAgggcccgcccgggtttt                                                                                                              >  1:88227/1‑227 (MQ=255)                                                                                                                                          tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCg                                                                                                     <  1:536291/250‑1 (MQ=255)                                                                                                                                             gcgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGt                                                                                                 <  1:333036/251‑1 (MQ=255)                                                                                                                                                 ccTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACTGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                                                                                             <  2:529821/251‑1 (MQ=255)                                                                                                                                                  cgtGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAATGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCCCCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCTGTATTAAACAGCCCGGCGTTgaag                                                                                            <  2:105650/249‑1 (MQ=255)                                                                                                                                                     gTAACGCTCTACCAACCAGCATGCCGCCTTCCCCGTGGTCTTTTGCCGCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCATTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGaaa                                                                                         <  2:73596/251‑1 (MQ=255)                                                                                                                                                         cgcgCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAgg                                                                                     <  1:235478/251‑1 (MQ=255)                                                                                                                                                           cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAgggg                                                                                   <  1:454800/251‑1 (MQ=255)                                                                                                                                                                ccaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCtttt                                                                              <  1:226473/251‑1 (MQ=255)                                                                                                                                                                        aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCgg                                                                      <  1:462967/251‑1 (MQ=255)                                                                                                                                                                           gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAtt                                                                   <  1:26173/251‑1 (MQ=255)                                                                                                                                                                           gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAtt                                                                   <  1:149784/251‑1 (MQ=255)                                                                                                                                                                            ccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAttt                                                                  <  1:7720/251‑1 (MQ=255)                                                                                                                                                                             cgccTTCACCGTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCATGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTc                                                                 <  1:35386/251‑1 (MQ=255)                                                                                                                                                                                 ttCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGt                                                                                                                                                    <  2:326408/164‑1 (MQ=255)                                                                                                                                                                                  tCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCg                                                             <  1:380996/250‑1 (MQ=255)                                                                                                                                                                                         ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCg                                                     <  1:52213/251‑1 (MQ=255)                                                                                                                                                                                                     cttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAACCCCCCAAGGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATATGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTa                                           <  2:338287/249‑1 (MQ=255)                                                                                                                                                                                                            gTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgct                                  <  1:159385/251‑1 (MQ=255)                                                                                                                                                                                                                          gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                    >  1:440436/1‑251 (MQ=255)                                                                                                                                                                                                                          gCAGGTCAAGGTCATGATGCGCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGCACGAACCCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGGCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTGTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                    <  2:56262/251‑1 (MQ=255)                                                                                                                                                                                                                           caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                   <  1:46344/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGGAGGGCCAAACAGCAGCTCGTACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCCGGTCGCGCCCGGGATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTTCGCCCGGGTTTCAGCGTAAACTCGCCAAACTTTAACCCCTGCTTGCTAAACGCCAATTCAATAAACTTGCGCTgt  >  1:66546/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGG‑TGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGA  >  NC_000913/3815272‑3815757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑