Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,466	T→C	E101E (GAA→GAG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,466	0	T	C	100.0%	187.3 / NA	55	E101E (GAA→GAG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/38);  total (17/38)

GTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGG‑TGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCG  >  NC_000913/3815221‑3815706                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gTGATGATAGAGATCACTTTGCAGTTGTAATNCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCttt                                                                                                                                                                                                                                              <  2:242777/251‑1 (MQ=255)    atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCg                                                                                                                                                                                                                                           >  1:348982/1‑251 (MQ=255)    atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGAGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCg                                                                                                                                                                                                                                           >  1:305713/1‑251 (MQ=255)      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCCCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATATACCAGCATTACGCGTCCTTGTAACGCGCTACCCACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                                                                                                                                                                                         >  1:536602/1‑251 (MQ=255)          gagaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTaa                                                                                                                                                                                                                                      >  1:249094/1‑250 (MQ=255)           agaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGTTTAAAg                                                                                                                                                                                                                                    <  1:477194/251‑1 (MQ=255)             aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCTATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCa                                                                                                                                                                                                                                  <  1:481802/251‑1 (MQ=255)             aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGc                                                                                                                                                                                                                                   >  1:142879/1‑250 (MQ=255)                 cTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTAc                                                                                                                                                                                                                              >  1:282294/1‑251 (MQ=255)                       aGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGCACGGcagg                                                                                                                                                                                                                        >  1:214732/1‑251 (MQ=255)                        gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGACGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAAACAGATTGACGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCaccacgcacggg                                                                                                                                                                                                                       >  2:57435/1‑240 (MQ=255)                           gTAATCCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTACAGCAGTACGGcaggtcaa                                                                                                                                                                                                                    <  2:451097/251‑3 (MQ=255)                                           tCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGGACGGcagggtaaaggcatgttttccccc                                                                                                                                                                                                    >  2:333036/1‑231 (MQ=255)                                              tGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCCGGACGGcaggtcgcggtcttgcttcttcccccg                                                                                                                                                                                                 >  2:380996/1‑230 (MQ=255)                                               gAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAATCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGGTAAAGCAGTACGGcagggaaagggcctgattctccccccgc                                                                                                                                                                                                >  2:328278/1‑227 (MQ=255)                                                 aTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATACTccccccgcgc                                                                                                                                                                                              >  2:462967/1‑243 (MQ=255)                                                    gCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAACCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCcac                                                                                                                                                                                           <  2:87614/251‑1 (MQ=255)                                                              cgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCAGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtag                                                                                                                                                                                   <  2:393086/249‑3 (MQ=255)                                                                   cgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCATGATGCTCCGCCAGCGCCAcggcgggagggggcgc                                                                                                                                                                            >  1:92318/1‑241 (MQ=255)                                                                         cgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCgg                                                                                                                                                                     <  2:92318/251‑1 (MQ=255)                                                                                 aCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCtt                                                                                                                                                             <  1:57513/251‑1 (MQ=255)                                                                                       aaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAg                                                                                                                                                       <  1:346883/250‑1 (MQ=255)                                                                                           gAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAgg                                                                                                                                                   <  1:206272/251‑1 (MQ=255)                                                                                                 aatacaccAGCAAGCGGCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCaaa                                                                                                                                             <  2:86325/245‑1 (MQ=255)                                                                                                        cAGCAAGCGTCCCGCCATTAGCCTGCATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCACCCGTATTTCCTCCTTCACCGTGGTCTTTTGCCTCTTTTCGGTTAAAGCAGTGCGGCAGGTCAACGTCATGATGCTTCGCCAGCGCCACGGCGGTAGTGT‑TCGCAATCGGTATCCCGTTGTAAGCAGGTCCAAAcagcagt                                                                                                                                      <  1:426872/251‑2 (MQ=255)                                                                                                          gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑ACGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATc                                                                                                                                    <  1:37491/251‑1 (MQ=255)                                                                                                               cGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACt                                                                                                                               <  1:147455/251‑1 (MQ=255)                                                                                                                 tCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGACTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATTATGCTCCGCCAGCGCCACGGCGGGAGTGGGTGGAAACTGGGA‑CCCTTTGTAAGCAGGGGCCAAcaggcaatggacctcc                                                                                                                             >  1:210915/1‑238 (MQ=255)                                                                                                                   gcgcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGG‑TCGCCAACGGGGTCCCCTTTTAAGGAGGGCCAAAAAGGCGATCGAACACaa                                                                                                                            >  1:249792/1‑250 (MQ=255)                                                                                                                               tGAATAATCTCCATCGATTCGCGAATCGCCGTTCAGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGCCAAGGGCATGATGCCCCGCCAGCGCCACGGCGGGTGGGG‑GCGCCAACGGGAACCCCTTGTAAACAGGGCCAAACAGCCGATAGAAcccacaacccgaatcccc                                                                                                               >  1:143015/1‑234 (MQ=255)                                                                                                                                    aatCTCCATCGATGCGCTAATCGCCGTTCCGGCGGTGATCACATCACCTACCATCAGTACGCGTCCTTGTAACGCTCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTTTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCTGTAGTGG‑TCGCAATCGGGATCCCTGTGGAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAAGCCCCCaatg                                                                                                          <  2:174347/251‑3 (MQ=255)                                                                                                                                                            cGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACa                                                                                  <  1:234449/251‑1 (MQ=255)                                                                                                                                                             gTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAg                                                                                 <  1:16742/251‑1 (MQ=255)                                                                                                                                                                    cggTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTCCCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAgg                                                                          <  2:58531/251‑2 (MQ=255)                                                                                                                                                                        gATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTcg                                                                       <  1:485528/250‑1 (MQ=255)                                                                                                                                                                                     aCCAGCATTACGCGTCCTTGGTACGCGCTTCCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACTGCCTAACAGTGCCAGGTCGCGCCCGGTAtt                                                            <  2:352733/248‑1 (MQ=255)                                                                                                                                                                                        aGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAg                                                      <  1:118606/251‑1 (MQ=255)                                                                                                                                                                                           aTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                           <  2:88227/243‑1 (MQ=255)                                                                                                                                                                                           aTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGG‑TCGCAATCGGGATCCCTTTTTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGCAAAAAAGGCCCAACAGTGCCAgggcccgcccgggtttt                                                           >  1:88227/1‑227 (MQ=255)                                                                                                                                                                                             tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCg                                                  <  1:536291/250‑1 (MQ=255)                                                                                                                                                                                                gcgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGt                                              <  1:333036/251‑1 (MQ=255)                                                                                                                                                                                                    ccTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACTGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                                          <  2:529821/251‑1 (MQ=255)                                                                                                                                                                                                     cgtGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAATGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCCCCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCTGTATTAAACAGCCCGGCGTTgaag                                         <  2:105650/249‑1 (MQ=255)                                                                                                                                                                                                        gTAACGCTCTACCAACCAGCATGCCGCCTTCCCCGTGGTCTTTTGCCGCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCATTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGaaa                                      <  2:73596/251‑1 (MQ=255)                                                                                                                                                                                                            cgcgCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAgg                                  <  1:235478/251‑1 (MQ=255)                                                                                                                                                                                                              cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAgggg                                <  1:454800/251‑1 (MQ=255)                                                                                                                                                                                                                   ccaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCtttt                           <  1:226473/251‑1 (MQ=255)                                                                                                                                                                                                                           aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCgg                   <  1:462967/251‑1 (MQ=255)                                                                                                                                                                                                                              gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAtt                <  1:26173/251‑1 (MQ=255)                                                                                                                                                                                                                              gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAtt                <  1:149784/251‑1 (MQ=255)                                                                                                                                                                                                                               ccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAttt               <  1:7720/251‑1 (MQ=255)                                                                                                                                                                                                                                cgccTTCACCGTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCATGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTc              <  1:35386/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGt                                                                                                 <  2:326408/164‑1 (MQ=255)                                                                                                                                                                                                                                     tCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCg          <  1:380996/250‑1 (MQ=255)                                                                                                                                                                                                                                            ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCg  <  1:52213/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGG‑TGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCG  >  NC_000913/3815221‑3815706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑