Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,364	C→T	E135E (GAG→GAA)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,364	0	C	T	100.0%	170.4 / NA	52	E135E (GAG→GAA)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

CGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGG‑TGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACG  >  NC_000913/3815118‑3815602                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                cGAGTTTGTTTAAACCCCAAACTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTGCTCTTCCAGATAGGCAATCAGAGCTTTCATGGTTATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTcgc                                                                                                                                                                                                                                             <  1:298208/251‑1 (MQ=255)       tCTTTAAACGCCAAACTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATc                                                                                                                                                                                                                                        <  2:386579/250‑1 (MQ=255)          ttAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGCAATCAATACGCCAGCAAGCGTCGCGCCATTAACCTGAATAATCTCCATCGATTCGCGAATCGCCg                                                                                                                                                                                                                                    >  1:18711/1‑251 (MQ=255)          ttAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCg                                                                                                                                                                                                                                    >  1:104023/1‑251 (MQ=255)                    aaCTCTTCGCGATAGTCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGt                                                                                                                                                                                                                          <  1:201112/251‑1 (MQ=255)                        ctTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGCGATc                                                                                                                                                                                                                      >  2:16742/1‑251 (MQ=255)                                  ggCCTTATCCGCCGCCAGATGTTCCGCCAGTTCCGGCTGCTCTTCCAGATAGGTAATCCGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAAGCGCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCAcctcatata                                                                                                                                                                                                            <  2:206380/251‑4 (MQ=255)                                             ccgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg                                                                                                                                                                                                 <  1:108668/251‑1 (MQ=255)                                                 cAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTc                                                                                                                                                                                             >  1:56262/1‑251 (MQ=255)                                                  aGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATACTCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCCCATCCTCTCCCAGccttccccgccc                                                                                                                                                                                            >  2:159385/1‑240 (MQ=255)                                                       ttCCGCCATTTCCGGCTTGTCTTCAAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGCTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGACGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGCCGCGCCATTAGCCTGAATAATATCCATCGATTCGCGACTCGCCGTTCCGGCGGTGACCACCTCATCTACCAGCATTACGCGTCCTTGtc                                                                                                                                                                                       >  2:454800/1‑250 (MQ=255)                                                               tttCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCTCAAATCGCCGTTCCGGCGGTGATCCCATCATCTACCAGCATTACGCCTCCTTGTAACGGGCtc                                                                                                                                                                               >  1:81965/1‑250 (MQ=255)                                                                    ggCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGCGATCACATCATCTAACAGCATTACGCGTCCTTGTAACGCGCTaccaac                                                                                                                                                                          >  2:346883/1‑251 (MQ=255)                                                                         ctctTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCCGTTGTAATCACCCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCAATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCAGTACGCGTCCTTGTAACGCCCTACCAACCCGAt                                                                                                                                                                     >  1:529821/1‑251 (MQ=255)                                                                             gccagataggCAATCAGATCTGTCAGGGTGATGATAGAGATCACTTTGCAGTGGTATTCACGCTCATCTCCCTGAATCGCCGAAATCCCGCCGCGCCCCCGTTCCTGACGATCAAGCGAACTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAACCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTCACGCGCTACCAACCAGAtt                                                                                                                                                                    <  1:231663/239‑1 (MQ=255)                                                                              ccagataggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATCACATCATCTACCCGCATTACGCGGCCTTGTAACCCGCTCCCAACCAGATTgccc                                                                                                                                                                >  2:26173/9‑250 (MQ=255)                                                                                     ggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCAc                                                                                                                                                         >  1:352733/2‑251 (MQ=255)                                                                                      gCAAACAGATCTCTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTGCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTCCCTGATGATCAAGAGAAATCAAGGCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGTTGATCGCATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCATGTTGCCGCCGTCAcc                                                                                                                                                        <  2:229767/251‑1 (MQ=255)                                                                                                cTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGAGGTCtttt                                                                                                                                              >  1:86325/1‑251 (MQ=255)                                                                                                        gTGATGATAGAGATCACTTTGCAGTTGTAATNCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCttt                                                                                                                                      <  2:242777/251‑1 (MQ=255)                                                                                                           atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCg                                                                                                                                   >  1:348982/1‑251 (MQ=255)                                                                                                           atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGAGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCg                                                                                                                                   >  1:305713/1‑251 (MQ=255)                                                                                                             gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCCCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATATACCAGCATTACGCGTCCTTGTAACGCGCTACCCACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                                                                                 >  1:536602/1‑251 (MQ=255)                                                                                                                 gagaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTaa                                                                                                                              >  1:249094/1‑250 (MQ=255)                                                                                                                  agaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGTTTAAAg                                                                                                                            <  1:477194/251‑1 (MQ=255)                                                                                                                    aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCTATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCa                                                                                                                          <  1:481802/251‑1 (MQ=255)                                                                                                                    aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGc                                                                                                                           >  1:142879/1‑250 (MQ=255)                                                                                                                        cTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTAc                                                                                                                      >  1:282294/1‑251 (MQ=255)                                                                                                                         tttGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGt                                                                                                                                                      <  2:186298/218‑1 (MQ=255)                                                                                                                         tttGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGt                                                                                                                                                      >  1:186298/1‑218 (MQ=255)                                                                                                                              aGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGCACGGcagg                                                                                                                >  1:214732/1‑251 (MQ=255)                                                                                                                               gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGACGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAAACAGATTGACGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCaccacgcacggg                                                                                                               >  2:57435/1‑240 (MQ=255)                                                                                                                                  gTAATCCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTACAGCAGTACGGcaggtcaa                                                                                                            <  2:451097/251‑3 (MQ=255)                                                                                                                                                  tCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGGACGGcagggtaaaggcatgttttccccc                                                                                            >  2:333036/1‑231 (MQ=255)                                                                                                                                                     tGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCCGGACGGcaggtcgcggtcttgcttcttcccccg                                                                                         >  2:380996/1‑230 (MQ=255)                                                                                                                                                      gAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAATCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGGTAAAGCAGTACGGcagggaaagggcctgattctccccccgc                                                                                        >  2:328278/1‑227 (MQ=255)                                                                                                                                                        aTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATACTccccccgcgc                                                                                      >  2:462967/1‑243 (MQ=255)                                                                                                                                                           gCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAACCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCcac                                                                                   <  2:87614/251‑1 (MQ=255)                                                                                                                                                                     cgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCAGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtag                                                                           <  2:393086/249‑3 (MQ=255)                                                                                                                                                                          cgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCATGATGCTCCGCCAGCGCCAcggcgggagggggcgc                                                                    >  1:92318/1‑241 (MQ=255)                                                                                                                                                                                cgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCgg                                                             <  2:92318/251‑1 (MQ=255)                                                                                                                                                                                        aCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCtt                                                     <  1:57513/251‑1 (MQ=255)                                                                                                                                                                                              aaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAg                                               <  1:346883/250‑1 (MQ=255)                                                                                                                                                                                                  gAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAgg                                           <  1:206272/251‑1 (MQ=255)                                                                                                                                                                                                        aatacaccAGCAAGCGGCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCaaa                                     <  2:86325/245‑1 (MQ=255)                                                                                                                                                                                                               cAGCAAGCGTCCCGCCATTAGCCTGCATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCACCCGTATTTCCTCCTTCACCGTGGTCTTTTGCCTCTTTTCGGTTAAAGCAGTGCGGCAGGTCAACGTCATGATGCTTCGCCAGCGCCACGGCGGTAGTGT‑TCGCAATCGGTATCCCGTTGTAAGCAGGTCCAAAcagcagt                              <  1:426872/251‑2 (MQ=255)                                                                                                                                                                                                                 gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑ACGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATc                            <  1:37491/251‑1 (MQ=255)                                                                                                                                                                                                                      cGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGG‑TCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACt                       <  1:147455/251‑1 (MQ=255)                                                                                                                                                                                                                        tCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGACTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATTATGCTCCGCCAGCGCCACGGCGGGAGTGGGTGGAAACTGGGA‑CCCTTTGTAAGCAGGGGCCAAcaggcaatggacctcc                     >  1:210915/1‑238 (MQ=255)                                                                                                                                                                                                                          gcgcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGG‑TCGCCAACGGGGTCCCCTTTTAAGGAGGGCCAAAAAGGCGATCGAACACaa                    >  1:249792/1‑250 (MQ=255)                                                                                                                                                                                                                                      tGAATAATCTCCATCGATTCGCGAATCGCCGTTCAGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGCCAAGGGCATGATGCCCCGCCAGCGCCACGGCGGGTGGGG‑GCGCCAACGGGAACCCCTTGTAAACAGGGCCAAACAGCCGATAGAAcccacaacccgaatcccc       >  1:143015/1‑234 (MQ=255)                                                                                                                                                                                                                                           aatCTCCATCGATGCGCTAATCGCCGTTCCGGCGGTGATCACATCACCTACCATCAGTACGCGTCCTTGTAACGCTCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTTTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCTGTAGTGG‑TCGCAATCGGGATCCCTGTGGAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAAGCCCCCaatg  <  2:174347/251‑3 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                CGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGG‑TGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACG  >  NC_000913/3815118‑3815602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑