Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	535,337	T→C	A474A (GCT→GCC)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	535,337	0	T	C	100.0%	269.1 / NA	74	A474A (GCT→GCC)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/39);  total (35/39)

ATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCT  >  NC_000913/535092‑535579                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aTTGGTCTGTCACAAATAGCTGCGGCACACATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTACAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCAGTACATCCATGTGCTGGTCAACAACGCCTATCt                                                                                                                                                                                                                                                >  1:384408/1‑251 (MQ=255)         tccacaactcgtggCGGCACAAATGCTGAAGGTCTTTAAAACCCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCt                                                                                                                                                                                                                                          <  2:136914/236‑1 (MQ=255)         gTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCAAGTTCCTGATTGCAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTgt                                                                                                                                                                                                                                        >  2:174539/1‑250 (MQ=255)         gTCACAAACCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTg                                                                                                                                                                                                                                         <  1:363517/250‑1 (MQ=255)            acaAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTc                                                                                                                                                                                                                                     >  2:463716/1‑251 (MQ=255)                aTCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCgtc                                                                                                                                                                                                                                  >  2:428637/1‑250 (MQ=255)                   gCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCCGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCgtcagtc                                                                                                                                                                                                                              >  2:227075/1‑251 (MQ=255)                        ggCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAc                                                                                                                                                                                                                         >  1:387452/1‑251 (MQ=255)                        ggCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAc                                                                                                                                                                                                                         >  1:375299/1‑251 (MQ=255)                         gCACAAATGCTGCATGTCTTTAAAGGCCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAcg                                                                                                                                                                                                                        <  1:358505/251‑1 (MQ=255)                           acatattttgctttgcTTTAAAGACCGCCGCTGGATCAAATGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAcgcg                                                                                                                                                                                                                      <  2:14040/236‑1 (MQ=255)                             aaaTGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCt                                                                                                                                                                                                                    >  1:201040/1‑251 (MQ=255)                                 gttgaaTGTTTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                                                                                                                                                <  2:196138/246‑1 (MQ=255)                                 ggtgatgggttttAAATACCGCCACTGGAGCAACTGGGGTCAGGCTGAGCCGTTAGGCGGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCGGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATGCCGTACAGCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTAACAACGCGCttgtg                                                                                                                                                                                                                <  2:216581/241‑4 (MQ=255)                                  ctgcATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                                                                                                                                                >  2:27711/1‑250 (MQ=255)                                    gcATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGAc                                                                                                                                                                                                              >  1:53331/1‑250 (MQ=255)                                             tAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGTCGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGAGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGGCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGactact                                                                                                                                                                                                    >  1:297655/1‑251 (MQ=255)                                             tAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGactac                                                                                                                                                                                                     <  1:125802/250‑1 (MQ=255)                                               aaGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGc                                                                                                                                                                                                  >  1:36043/1‑251 (MQ=255)                                                aGACCGCCACGTGATCATCGTTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGGTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCg                                                                                                                                                                                                 <  2:48444/251‑1 (MQ=255)                                                  aCCGCCACGGGATCAACTGTGGTCAGGCTGGTCCGTTAGTCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCCCAACGCGCTTTTGACATGGACTACTGCGTg                                                                                                                                                                                               <  2:369262/251‑1 (MQ=255)                                                       cACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAAcc                                                                                                                                                                                          >  2:116560/1‑250 (MQ=255)                                                           ggATCAACTGTGGTCGGGCTGGTCCGTTAGGCGGGACGAGTCCGGCTGCGCTAGGTGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGAGTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATGCCGTACATCCATGTGCTGGTCAACAACGCCGATCTGGGGCTGAGTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGc                                                                                                                                                                                       <  2:521136/250‑1 (MQ=255)                                                                      ggtgaGGCTGGTCCGTTAGTCTTGCCGATTCCGGCTGCGCTAGGGGTTTGTGGCGCTGTTCCGAATCGCAATGTGTTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGGTGGCGCGCAGTTCAACATGCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACTCTCTTGTGACATGGAGTACGGCGTGCAACTCGCTTGCTAGAatat                                                                                                                                                                           <  2:309913/247‑1 (MQ=255)                                                                       gTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCTGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATc                                                                                                                                                                          >  1:502544/1‑251 (MQ=255)                                                                               tgTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGGTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGAGTCGTCAGTCACAACGCGCTGTTGAGATGGACTACTGCGTTCAACTCGCTTTCGAGAATATCAACTCCa                                                                                                                                                                   <  2:39297/249‑1 (MQ=255)                                                                                gTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagt                                                                                                                                                                 >  2:34932/1‑251 (MQ=255)                                                                                       aGGCTGGTTGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCGGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaagtg                                                                                                                                                           <  2:502544/250‑1 (MQ=255)                                                                                              aCGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACCTTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtg                                                                                                                                                                >  2:189170/1‑238 (MQ=255)                                                                                              aCGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGGGCAACTGGCTTTCGAGAATATCAACTCCagtg                                                                                                                                                                <  1:189170/238‑1 (MQ=255)                                                                                                      ggCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTg                                                                                                                                          <  2:531283/251‑1 (MQ=255)                                                                                                           cgcTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAAGATCAGCTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCAc                                                                                                                                     <  1:128171/251‑1 (MQ=255)                                                                                                               ggtggTTTGTGCCGCTGAGCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGGCTTCCATTTCCTGAGTGATGTGTTAGCTTTTGGCGCGCAGTTCGACATTCCGTACATCCATGTGCTCGTCAACAACGCCGATCTGGGGCTGATTCTTCAGTCACAACGCGCTCTTGACATGGACTACAGCGGGCAGCACGTTTGCGAGAATATTAACGCTATATAAGTGAATGGCT‑ACGGTGTGGGCCACGTat                                                                                                                                 <  2:204849/248‑2 (MQ=255)                                                                                                               aGGGGTTTGTGCCGGTGATTCTAAACGCAAGNTTGGGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACGGTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGGTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGGTGCCCACGTaa                                                                                                                                 <  2:270962/251‑1 (MQ=255)                                                                                                               aGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGTT‑ACGGTGTTGACCACGt                                                                                                                                   <  1:174223/249‑1 (MQ=255)                                                                                                                  ggTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCACCATTCCGTACATCAATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCAAGTGAAGTGAATGGCT‑ACGGTGTTGACCACgacaa                                                                                                                                >  2:20423/1‑245 (MQ=255)                                                                                                                  ggTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGCATGGCC‑ACGGTGTTGAccccggaaa                                                                                                                                >  2:359432/1‑242 (MQ=255)                                                                                                                        ggCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCgg                                                                                                                        <  2:201040/250‑1 (MQ=255)                                                                                                                                   cGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCGGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGt                                                                                                             <  2:406732/251‑1 (MQ=255)                                                                                                                                    gAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGtt                                                                                                            <  1:34932/251‑1 (MQ=255)                                                                                                                                    gAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGtt                                                                                                            >  1:291619/1‑251 (MQ=255)                                                                                                                                              gtggtgGCGATTTCTTGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTCGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTGTGGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTGTAGGGTGTAAAGCTAt                                                                                                  <  2:384408/251‑1 (MQ=255)                                                                                                                                                         ttCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTc                                                                                        <  1:370953/250‑1 (MQ=255)                                                                                                                                                         ttCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTc                                                                                        <  1:208790/250‑1 (MQ=255)                                                                                                                                                             ggCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGCAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAAcc                                                                                   >  1:348553/1‑251 (MQ=255)                                                                                                                                                                    ttGACTACAAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGCCTACTGCGTGCAACTCGCTTTCGAGAATATCACCTCCAGTGACGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGGTGTAAAACTATACGGGTCTTCACACCGGacga                                                                             >  2:192142/1‑247 (MQ=255)                                                                                                                                                                       aCTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTACAGCTATTCGGGTCTTCAAACCGGAAGATAtt                                                                         >  2:69224/1‑251 (MQ=255)                                                                                                                                                                        ccgccAGTTCCTTATTGAAGATGTAGGTTTTGGCGCGCAGTTCAACATTCCTTTCCTCCAAGTGCTGGTCAACCAAGCCTGTCTGGGGGTGATTCGTCAGTTAATACGTCTTTTTGACATTGACTACTTCGTGCCACTCCTTTTCCAAAATTTTAACTCCCGTGAAGTGAAAGGTTTAGGGT‑TTGTCCACGTATAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGaacctattg                                                                        <  2:118435/248‑8 (MQ=255)                                                                                                                                                                            cAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGcc                                                                    <  1:457809/251‑1 (MQ=255)                                                                                                                                                                                    gATTGACGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTAGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTACGCCAGCCTTTg                                                            >  2:57569/1‑251 (MQ=255)                                                                                                                                                                                    gATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTg                                                            >  1:540930/1‑251 (MQ=255)                                                                                                                                                                                    gATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTg                                                            <  1:359432/251‑1 (MQ=255)                                                                                                                                                                                        gAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAAc                                                         >  1:318439/1‑250 (MQ=255)                                                                                                                                                                                          agagTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAgg                                                      >  1:158992/1‑251 (MQ=255)                                                                                                                                                                                             gTTAGCTGTTGGCGCGCAGTTCAACCTTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTAGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGGAAAAGTAGCGGACGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAAAAGGCg                                                    >  2:303113/1‑250 (MQ=255)                                                                                                                                                                                             gTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCg                                                    >  1:271059/1‑250 (MQ=255)                                                                                                                                                                                               tAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGaa                                                  <  1:418732/250‑1 (MQ=255)                                                                                                                                                                                               tAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAAACGGAAAATATTGCGCCAGCCTTTGAACAGGCGaa                                                  >  2:311339/1‑250 (MQ=255)                                                                                                                                                                                                   tGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCt                                             >  1:202930/1‑251 (MQ=255)                                                                                                                                                                                                    gTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCt                                             <  1:463716/250‑1 (MQ=255)                                                                                                                                                                                                       ggCGCGCATGTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGTAAGATATTGCGCCAGCCttt                                                             <  2:101057/231‑1 (MQ=255)                                                                                                                                                                                                       ggCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCttt                                                             >  1:101057/1‑231 (MQ=255)                                                                                                                                                                                                         cgctcAGTTCAACATGCCGTTCCTCCATGTGTTGTTCAACAACGCCTAATTGGGGCTGAGGCGTCAGTCACAACGCGCTTTTGACATGGACTATTGCGTGCATCTCGGTTTCGAGCATATCAACTCCAGTGAAGTGAATGGCT‑GCGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGTTTGTCAAGCTATTCGTGTCTTCAAACCGGAAGTTAGTGCGCAAGCCTTGTAACAGGCGAAAGCCTTAATgg                                       <  2:407071/247‑1 (MQ=255)                                                                                                                                                                                                                  cAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACCTGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCt                                                                                          >  2:115109/1‑191 (MQ=255)                                                                                                                                                                                                                  cAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCt                                                                                          <  1:115109/191‑1 (MQ=255)                                                                                                                                                                                                                    aCATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAAc                                                                                    >  2:435512/1‑195 (MQ=255)                                                                                                                                                                                                                    aCATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAAc                                                                                    <  1:435512/195‑1 (MQ=255)                                                                                                                                                                                                                    aCATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCgg                            <  1:227075/251‑1 (MQ=255)                                                                                                                                                                                                                        tCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTAc                        >  2:399323/1‑251 (MQ=255)                                                                                                                                                                                                                               aTCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGt                 <  1:475197/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGCCCACGTAAAAGTAGCGGAAGGTTTAGGATGTAAAGCTATTCGGGTCTTCAAACCGGCAGATATTGCGCCAGCCTTTGAACAGGCGCAAGCCTTAATTGCGAAATATCgggacccggtggacg               >  2:287877/1‑239 (MQ=255)                                                                                                                                                                                                                                     gtgCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTgg            <  2:187732/250‑1 (MQ=255)                                                                                                                                                                                                                                           gTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTAt     <  1:91063/251‑1 (MQ=255)                                                                                                                                                                                                                                              aacaacGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATtct  <  2:486955/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCT‑ACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCT  >  NC_000913/535092‑535579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑