| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,135,781 | 0 | G | T | 29.8% | 75.0 / 16.4 | 57 | R197R (CGC→CGA) | pitB | metal phosphate:H(+) symporter PitB |
| Reads supporting (aligned to +/- strand): ref base G (11/29); new base T (16/1); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAGGCCGGTA > NC_000913/3135543‑3136025 | gCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑a < 1:308900/251‑1 (MQ=255) cTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑ATTA‑CCGTTGCCCTTTTTTTTTTTGCgtgattccggg > 1:38355/1‑240 (MQ=255) ggTAGAGTTCGCAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGAATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAACACGCCACGCCCGCCGCGGAAACAATCAGCGCACTACGCGTCCAGAATGGCGG‑TTTA‑TGGTTTCAATTTTTCTTTTTTCATTCTTCC‑ggcctgcggggg > 2:306944/1‑241 (MQ=255) aGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTTCGGTCTTCC‑GG‑AAAGCgggggat > 1:533661/1‑245 (MQ=255) gTAACGGCATCGCGGGTTCGGGAAATTTCAGAGCCGGACGCATTCATAGTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTGGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCttttt < 2:105215/251‑1 (MQ=255) aCGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑GTTT‑CGTTTGCCTTTTTTCTTTTTGCGGTCTTCC‑GG‑GATGCGGGGAATaagggtacccttcttcgt > 2:93251/1‑234 (MQ=255) cATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTAACGTTT‑CCTTTTTTTTTTTTGAGAACTTCC‑GG‑AATGCGGTGAATAAGGT‑CAC‑GGTTTTTTGTcccc > 1:390767/1‑250 (MQ=255) gggtacgggtaATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTTTTTTTTTGATTTTCC‑GG‑GAGTCGGTGAATACGGT‑‑ACAGCTTTTTTGTCCCGCTcccg > 1:93294/1‑249 (MQ=255) ggtaATTTCATAGCCTGACTCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTc < 2:143232/251‑1 (MQ=255) gtaATTTCATAGCCGGACGCATTCAGAGTGACGACGAAGCCAGCAGGGGCAAGCCCCTCCAGTACCAGCATTACCAGGCCGATCCCTGTTGGTCCGTCGTGCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCTCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTGGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGGGAATACGGT‑CAC‑GCTTTGTCGTCCCGCTCCAGTAGCGTCg < 2:136894/251‑1 (MQ=255) aaTTTCATAGCCGGACTCATTCATATTGACGACGAAGCCAGCAGGGGCAAGCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCATCCCGCTCCAGTAGCGTCgca < 2:425773/251‑1 (MQ=255) aTTTCATAGCCGGACGCATTCATATTGACGGCGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCgcag < 1:38070/251‑1 (MQ=255) ttCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑GATGCGGGGGATACGGG‑CAC‑GCTTTTTTGTCCCGCTCCAGTAGCGTCgcagcc > 1:160868/1‑250 (MQ=255) cATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAgg < 1:486652/251‑1 (MQ=255) cGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CCTTTTCCTTTTTTTTTTTTGCGATCTTCC‑CG‑GATGCGGTGAATACGGT‑CAC‑GCTTTTTTGTCCCGCCCCCGTAGCGTCGCAGCCGGAAAATc > 1:184005/1‑251 (MQ=255) ggACGCATTCATATTGACGACGAAGCCAGCAGGGGCAAGCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCa < 2:75763/251‑1 (MQ=255) aCGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAgg < 2:160868/251‑1 (MQ=255) gCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGGTTGCCTTTTTTTTTTTTGCGATTTTCC‑GG‑AATGCGGGGAATAAGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTCGCGTCGCAGCAGGAATATccagcc > 1:476079/1‑246 (MQ=255) aatgaagacgaAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGGATGACTTTTTTCTTTTTTCGACCCTCC‑GGGAAAG‑GGTGAATACGGG‑CAA‑GTTATTTAGGCCCGCTCCAGTAGCGTCTCAGCAGGAATAACAGGGCTCCCGCa > 2:150729/7‑250 (MQ=255) ttGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGGTTTC‑GGTTGGCCTTTTTTTTTTTTTGGA‑CTTCCCGG‑AA‑GCGGTGGAAGCGGG‑CAC‑CCTTTTTCGTCCCGCTCCCGGAGCGTCGCCGCAGGAATATCAGGccccccccc > 1:493595/1‑246 (MQ=255) tgccgacgaAGCCAGCAGGGGCAATCCCCTCCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑t < 2:138149/247‑1 (MQ=255) atcgcgaAGCCCGCAGCTGCAATCCCCACCATTTCCAGCATTACCAGGCCGAGCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCATCGGAAACAATCATCTCAATACGCGTCCAGAATTGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCTTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGAc < 2:295342/247‑1 (MQ=255) acgaAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCCTTTTTTTTTTTTCGGTCTTCC‑GG‑GATGCGGGGGAAAAGGT‑CAC‑GCTTTTTTGTCCCCCTCCCGGAGGGTCGCCGCAgggaaaacaggccccccccacacgac > 1:338362/1‑225 (MQ=255) cgaAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATACCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑GTTA‑AGGTTGGCTTTTTTTTTTTTGCGGTCTTTC‑GG‑AATGCGGGGCAAACGGT‑CAC‑CCGTTTTTGTCCCCCTCCAGGAGCGTCGCAGGAGGGATATCAGGGCTCCCCCaaaggccaa > 1:295342/1‑243 (MQ=255) aGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑AATGCGGGGGATACGGT‑CAC‑GCTTTTTCGTCCCCCTCCAGTCGCGTCGCAGCAGGGAAATCAAGCCTccccccaagaaccggccc > 1:173866/1‑236 (MQ=255) agGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTTTTTTTTCGATCTTCC‑GG‑GATGCGGGGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCCCTAGCGGCGCAGCAGGGATATCAGGGCCCCCCCAA‑TGAcccagcccacggt > 1:484540/1‑238 (MQ=255) gggCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCCGGCCTCCCGCAA‑TGACCCGGCCGCCGATAggg > 1:96853/1‑251 (MQ=255) ccAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCgaggag < 1:477420/251‑1 (MQ=255) tACCAGGCCGATCCCGTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGaaagtt < 1:162324/250‑4 (MQ=255) aCCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑AATGCGGGGAATACGGG‑CAC‑GCCTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGGATATCAGGCCTCCCGCCA‑AGACCCGGCCGACGGGAGGGGAAACAAACACCGAGGAGAAAAattt > 1:40734/1‑251 (MQ=255) aCCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAAtttt < 1:37611/251‑1 (MQ=255) aTCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGGGAATACGGT‑CAC‑GCTTTTTTGGCCCCCCCCCGTAGCGTCGCCGCAGGGAAATCAGGCCCCCCGCAAATG‑CCCGGCCGACGGTAGGGGAAACAATCCACGCGGGGAAAATTTTGGTC‑ACTTc > 1:182079/1‑251 (MQ=255) gTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGttt < 1:77034/251‑1 (MQ=255) cgtcgtTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑AATGCGGGGGATACGGT‑CAC‑GCTTTTTTGTCCCGCCCCAGTAGCGTCGCAGCAGGGATATCAGGCCTCCCGCCA‑TGACCAGGCCGACGAAAGGGGAAACAATCAGCGGGGGGAAAATTTTGGGC‑ACTTCACGCAGGGTTAAc > 1:233317/1‑251 (MQ=255) gtcgtTCGCGCCGTGCGAAAACGCCACGCCCGCATCTGAAACAATCAGCGCAATACGCGTCCAGAATTGCGG‑TTTA‑CGTTTGCCTTTTTTCGTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACg < 1:60045/251‑1 (MQ=255) gtcggtcgCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAAGCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑ACTGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTGCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACg < 2:395741/251‑1 (MQ=255) cgtTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGca < 1:401812/251‑1 (MQ=255) cgtTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGc < 1:196448/250‑1 (MQ=255) gtTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGTTCCC‑CCTTTTT‑GTTCCGCTCCCGTAGCGCCGCCGCAGGAAAATCAGGGCTCCCCCCA‑TGACCCGGCCGACGATAGGGGAAACAAATAGCGAGGGGAAAATTTTgggcaactctcgccggctttaaacac > 1:64369/1‑227 (MQ=255) gtTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGcat < 1:489522/251‑1 (MQ=255) gtTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGcat < 2:230021/251‑1 (MQ=255) cGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTTTTTTTTCGATCTTCC‑GG‑GATGCGGTGGATACGGT‑CCC‑GCTTTTTCTTACCGCCCCACAAGCGGCGCCGCCGGAATATTAGGCCTCCCCCCA‑TGACCCGGCCGACGATAGGGGGAACAAACAGCGAGGCGAAAATTTTGGGC‑ACCTCACGCAGGGTTAACGcagccaacccc > 2:383133/1‑246 (MQ=255) gccacgccCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑AGTTTGCCTTTTTTCTTTTTTAGATCTTTC‑GG‑AATGCGGTGAATACGGG‑CAC‑GCTTTTTCGTCCCCCCCCAGTAGCGGCGCAGCAGGAATAAAAGGCCTCCCGCAA‑TGACCCGGCCGACGGTAGGGGAAAAAATCAGCGAGGAGAAAATTTTGGTC‑ACCTCACGCAGGTTTAAAGCATCCATCACCGATGAGCCggg > 1:483047/1‑250 (MQ=255) ccacgccCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGT‑TTTG‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTGTAACGCATCCATCGCCGATGAGCCGGtt < 2:503553/251‑1 (MQ=255) cAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGGGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAAAATTAGGCCTCCCCCAA‑TGACCAGGCCGACGAAAGGGGAAACAAACAGCGGGGAGAAAAATTTGGGC‑ACTTCACGCAGGGTTAACGCATCCATCACCGCTGAACCGGTTTACAAcgcg > 1:173621/1‑251 (MQ=255) cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTg < 1:410543/251‑1 (MQ=255) cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑AGGTTGCCTTTTTTCTTTTTTCGATCTTTC‑GG‑GATGCGGTGAATAAGGG‑CAC‑GCTTTTTTGTCCCCCCCCCTTAACGGCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGAACAGGGCGCCGGGAGGGGGAACAAAAAGCGGGGGGAAAAATTTTGTG‑ACTTCTCGCAGGTTTAAAACATCCATCACCGCTGCGCCGGTTataacggcggtg > 2:168359/1‑240 (MQ=255) aaaCAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGtt < 1:481596/251‑1 (MQ=255) gAATGGCGG‑TTTT‑CGTTTGCCTTTTTTCTTTTTTCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCCGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCCACGATAGGGGAAAAAAACAGCGAGGAGAAAATTTTGGTCCACTT‑ACGCAGGTTTAAACCCTCCATCACCGATTAGCCGGTTAACAGCGCGGTGGTTAAAACGACGcccgaaatcgccccac > 2:498237/1‑237 (MQ=255) gAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCaa < 1:168359/251‑1 (MQ=255) aaTGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAAt < 2:533661/251‑1 (MQ=255) a‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCg < 1:453172/251‑1 (MQ=255) ttGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAAGACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAAc < 2:184005/250‑1 (MQ=255) tGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACt < 1:93251/250‑1 (MQ=255) gCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTgg > 1:244298/1‑251 (MQ=255) tttttCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGGCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAg < 2:517154/249‑1 (MQ=255) tttttGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAGGCCGGTa > 1:358629/1‑250 (MQ=255) | GCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGG‑TTTA‑CGTTTGCCTTTTTTCTTTTTGCGATCTTCC‑GG‑AATGCGGTGAATACGGT‑CAC‑GCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAA‑TGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTC‑ACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAGGCCGGTA > NC_000913/3135543‑3136025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |