Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,377,1600CA20.0% 112.3 / 10.8 65A145S (GCC→TCC) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (42/10);  new base A (1/12);  total (43/22)
Fisher's exact test for biased strand distribution p-value = 1.72e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCAT  >  NC_000913/2376910‑2377408
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                         
gtaaTGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGc                                                                                                                                                                                                                                                           <  1:89310/249‑1 (MQ=255)
 gAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCa                                                                                                                                                                                                                                                          <  1:244361/251‑1 (MQ=255)
     gACATGGCATTCGGCAGCCAGGTCCGCCGCAAGGGCGCGGAATGTGCGGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGATAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGAGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGAAAAAACAGGCTGTTGATACCAGTC‑GGCAAAt                                                                                                                                                                                                                                                       <  2:132146/250‑1 (MQ=255)
       cATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGATG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAANAACAGGCTGTTGATACCAGTC‑GGCAAATAcc                                                                                                                                                                                                                                                    >  2:246304/1‑251 (MQ=255)
        aTGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATAccg                                                                                                                                                                                                                                                   <  1:341944/251‑1 (MQ=255)
          ggCAGTCGGCAGCCATTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATAccgcc                                                                                                                                                                                                                                                 <  2:143834/251‑1 (MQ=255)
                 ggCAGCCAGTTCAGCCGCCAGGGCGCGCAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTCAGGTTAGCACGTAAATCAGGCTGGACGGAGAGAGAAGTCGCCTCC‑AGCATAGCGGCAAGCGTAG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGGTGATCGTCATTTAGTGAGGCAAAAACAGGCTGTTGCTACCAGTC‑GGCAACTACCGCCGTTAACg                                                                                                                                                                                                                                          >  2:331035/1‑251 (MQ=255)
                   cAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGt                                                                                                                                                                                                                                        >  1:426179/1‑251 (MQ=255)
                        aGTTCAGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACGACATAAATAATAAAACGCAAA‑AGTGCGGG‑CGCTAAGGTTAGCACGAAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGAGACAAGCGTTG‑CGCCCTTATTGTTG‑ATGCGCAGCGACACCAGATCCCGGCGGTGATCGTCCTAGAGTGAGGCAAACACAGGCTGCTGGTACCAGGC‑GGCAAATACCGCAGTTacacgttctgg                                                                                                                                                                                                                                   >  2:425542/1‑241 (MQ=255)
                             cgccgcCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTgaga                                                                                                                                                                                                                               >  1:290257/1‑250 (MQ=255)
                                    aGGGCGCGGCATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCCTTATTGTTG‑CAGCGCCGCGCCACCAGCTCCCGGCGTTGATCGGCATTGAGTGAGGCAAAAACAGGCTGTGGTTACCAGTC‑GGCAAATACCGCCGGTAACGGTTCTGGGAGAAAAGGCTg                                                                                                                                                                                                                       >  2:409788/1‑251 (MQ=255)
                                     gggCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGc                                                                                                                                                                                                                      >  1:196490/1‑251 (MQ=255)
                                     gggCGCGAAATTTGCTGTCCCGTTAACCAAAAAAAAAATAAAACG‑AAA‑TGGGGGGGGCGTAAAGGTCGGCCCTTAAATCAGGCGGGCGGGCGAGAAAAG‑CGCCCCCCAGCAGGGCGGAAAGGGTTG‑CGCTATTATGGTTG‑CGGCGCAGCGCCCCCAGCTCCCGGCGTTGTTCGTCATTGAGGGGGACAAAAACAGGCGGTTGATCCCAGTC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGc                                                                                                                                                                                                                      <  2:280032/251‑1 (MQ=255)
                                       gggcgGAATTTGCTGTCACGTTCACCACATAAAAAATAAAACGAAAA‑TGTGCGGG‑CGCTAAGTTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCCCC‑AGCAGGGCGGAAAGCGTTG‑CGCCATTTTTGTGG‑CTGCGCAGCGCCCCCAGCTCCCGGCGTTGATCGTCATTGAGTGAGAAAAAAACAGGCTGTTGATCCCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCAc                                                                                                                                                                                                                    <  1:533426/249‑1 (MQ=255)
                                             aaTTTGCTGTCACGTTCACCATATAAATAATAAACCGAAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGAAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCTTGGCGGCAAGCTTTG‑CGCCATTATTGTTG‑CGGCGCGGCGCCACCAGCTCCCGGCGTTGTTCGTCATGGAGTGAGGAAAAAACAGGCTTTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTg                                                                                                                                                                                                              <  1:85615/251‑1 (MQ=255)
                                             aaTTTGCTGTCACGTTCACCACATAAATCATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCCAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGAAAATAACGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTg                                                                                                                                                                                                              >  2:402188/1‑251 (MQ=255)
                                              atttgttgtaatgttaacaaaataaataaAAAAACGCAAT‑TGGGCGGG‑GGCTAAGTTTCGCTGTTAAACCAGGCGGTACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGAAAGCGTTG‑CGCCTTTATTGTTG‑CTGCGCGGCGCCACCAGCTCCTGTGGGTGATCGCCATTGAGGGAGGAAAAAACAGGCGTTTGATACTATTC‑GGAAAACACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTgg                                                                                                                                                                                                             <  1:213574/230‑1 (MQ=255)
                                              agttGCTGCCACGTTCACCACAAAAATAA‑AAAACCCAAAATGGGCGGG‑GGCAAAGGTTAACACGTAAACCAGGTGGGCCGGGGAGAGAAGCCGCCTCC‑AGCGGGGCGGCACGCGTGG‑CGCCATTAT‑GTTGGCTGCGCAGCGCACCCAGCCCCCGGCGTTGATCGTCTTGGGGGGAGGCAAAAACAGGTGGTGGATCCCGGCC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTgg                                                                                                                                                                                                             <  2:77716/249‑1 (MQ=255)
                                              aTTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTgg                                                                                                                                                                                                             >  1:113921/1‑251 (MQ=255)
                                                 ggCTGTCACGTCCCCCAAAAAAATAAAAAAACGCAAA‑TGGGCGGG‑CGCTAAGGTTAGCACGTAAAACAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCTTTG‑CGCTTTTTTTGTTG‑CTGCGCAGCGCCACCCGCTCCCGGCGTTGACCGTCATTGAGTGAGGAAAAAACAGGCTTTGGATACCAGTC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGa                                                                                                                                                                                                          <  1:434552/250‑1 (MQ=255)
                                                  gCTGCCACGTTCCCCACATAAAAAAAAAAAGACAAA‑TGTGCGGG‑CGCTAAGTTTAGCACTAAAACCAGGCGGGACGGCGAGAGAAGTCGCCCCC‑ACCATGGCGCCAATCTTTG‑CCCCATTATGGTTG‑CTGCCCGGCGCCACCCTCCCCGGGCTTTAATCGTCATTGAGGGAGGAAAAAACGGGCTGTTGATACCAGGC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGAt                                                                                                                                                                                                         <  1:477645/251‑1 (MQ=255)
                                                    tGTCACGTTCACAACATAAATAAAAAAACGCAAA‑TGGGCGGG‑CGTTAAGTTTAGCACGTAAACCAGGCGGGCCGGGGAGAGAAGCCGCCCCC‑AGCATGGCGGCGAGCGTTG‑CGCCTTTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACGGGTTTTTGATCCCAGTC‑GGAAAATACCGCCGTTAACGGTTCTATGAGAAAACGCTGCACCCATTGGCGATc                                                                                                                                                                                                        <  1:50304/250‑1 (MQ=255)
                                                       cACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGa                                                                                                                                                                                                     >  1:8607/1‑250 (MQ=255)
                                                             caccacATAAATAATAACACGCAAA‑TGTGCGGG‑CGCTAAGGTTCGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTg                                                                                                                                                                                               >  2:25399/1‑250 (MQ=255)
                                                             caccacAAAAATAATAAAACGCAAA‑TGTGCGGG‑CGATAGGGTTAGCCCGTAAATCAGGCTGGCCGGCGAGAGAGGTCGCCTCC‑AGCATGGCGCCAGGCGTGG‑CGCTTTTTTTGTTG‑CTGCGCAGCGCCCCCAGCTCCCGGCGTTGATCGGCTTTGGGTGAGGCAAAAACAGGCTGTGGATACCAGTG‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTg                                                                                                                                                                                               <  1:409788/250‑1 (MQ=255)
                                                                 acaTAAATACTAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGtt                                                                                                                                                                                          >  1:427216/1‑251 (MQ=255)
                                                                 acaTAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGtt                                                                                                                                                                                          >  1:29239/1‑251 (MQ=255)
                                                                  aaaaaataaAAAAACGCAAA‑GGTGCGGG‑CGATAAGTTGAGCGCGTAAAGCAGGCTGGGCGGCGAGAGAGGCCGCCTCC‑AGCATGGCGGCAAGCGTGG‑CGCTATTATTGTGG‑CGGCGCCGCGCCCCCAGCCCCCGGCTGGGATCGCTATTGATTAAGGAAAAAACAGGCGGTGGATCCGAGTC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTc                                                                                                                                                                                         <  2:79049/248‑1 (MQ=255)
                                                                   ataaataaTAAAACGCAAA‑TGTTCGGG‑CGCTAAGGTTAGCAAGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGGTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGAACGGAACGCTGAAGTTcc                                                                                                                                                                                        >  1:360203/1‑251 (MQ=255)
                                                                      aataaaAAAACGCAAA‑TGGGCGGG‑CGCTAAGGTAAGCACGTAAATCAGGCTGGACGGCGAGAGAAGCCGCCTCC‑AGCATGGCGGCAAGCTTTG‑CGCCATTATTGTTG‑CGGCGCAGCGCCACCAGCTCCCGGGGTTGATCGTCATTGAGTGAGGAAAAAACAGGCTGTTGATACCAGTC‑GCCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCa                                                                                                                                                                                     <  1:313195/251‑1 (MQ=255)
                                                                       ataataAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGGGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCAc                                                                                                                                                                                    >  2:114901/1‑251 (MQ=255)
                                                                              aaagCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGCGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGAGCGTCATTGGGTGAGGCAAAACAAGGCTGTTGCTCCCAGTC‑GGCAAATACCGCCGTTACCGGTACTGTGAGACAACGCTGCACCCATTAGCGCTCGGCACGCGGACGTTCCGccaggtgttc                                                                                                                                                                             >  2:109604/4‑242 (MQ=255)
                                                                              aaCGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTc                                                                                                                                                                             >  1:399796/1‑251 (MQ=255)
                                                                                        gtgCGGG‑CGCTAAGGTTAGCACGAAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCCTTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTGGATCCCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATAGGAACGCTGACGTTCCGCAGGTTGTTCAGCAGTTTg                                                                                                                                                                    >  2:89524/1‑251 (MQ=255)
                                                                                          gCGGG‑CGCTAAGGTTAGCCCGTAAATCAGCATGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGAACGAGTTG‑CTCCATTATTGTTG‑CTGCGTAGCGCAAACAGCTCCCGGCGTTGATCGTCATTGAGTGCGGCAAAAACAGGCTGTTGATACCAGGC‑GGCAAATACCGCCGTTCACGGTTCTGTGAGAAAACGCTCCAACCAATGGCGATTGGAACGCTGTCGTTCAGCAAGTTGTTCAGCCtttttc                                                                                                                                                                   >  2:374282/1‑248 (MQ=255)
                                                                                           ggtgtgctctgccgatctaCGTAAAGCGGGCTGGAGGGGGAGAGAAGTCCCCCCC‑AGCAGGGCGGAAAGCGTGG‑CGCCTTTTTTGTTG‑CTGCGCAGCCCCACCACCCCCCGGGGTTGATCGTCATGGAGTGAGGAAAAAACAGGTTTTTGATCCCAGTC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCa                                                                                                                                                                  <  1:244972/233‑1 (MQ=255)
                                                                                              g‑cgcTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGccc                                                                                                                                                              >  1:141324/1‑251 (MQ=255)
                                                                                                ggcTAAGGTTTGCACGTAAACCGGGCGGGGCGGCGAGAGAGGCCCCCTCC‑AGCATGGCGGAAAGGGTTG‑CTCAATTTTTGTTG‑CTGCGCGGCGCCCCCACCTCCCGGCGTTGATCGTATTTGAGTGAGCAAAAAACAGGCTGTTGATCCAAGGC‑GGAAAAGCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGcccc                                                                                                                                                             <  1:477580/250‑1 (MQ=255)
                                                                                                    aagtgtacctcGTAAATCCGGCTGGCCGGGGAAAGAGGTCGCCTCC‑CGCTGGGCGGCAAGCGTTG‑CGCCATTATGTTTG‑CGGCGCAGCCCCCCCAGCCCCCGGCTTTTATCGTCTTTGAGGGAGGCAAAAACAGGCGGTTGACCCCGGTC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGAt                                                                                                                                                         <  1:402188/241‑1 (MQ=255)
                                                                                                      ggTTACCACATAACTCGGGCGGGACGGCTAGATAGGTCCCCTCA‑AGCGTGGGGGCAAG‑GTTGGCGCCTTTATTGTTG‑GTGCGCAGCGCCCCCATCTCCCGGCGTTGATCGCCATTGGGGGGGGAAAAAACAGGCTGTTGATACC‑GTCCGACAAATCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGc                                                                                                                                                       <  2:102117/251‑1 (MQ=255)
                                                                                                       gTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGcc                                                                                                                                                      >  1:125383/1‑251 (MQ=255)
                                                                                                            cACGAAAATCCGGCTGGACGGCGAGAGAAGTCGCATCC‑ATCATGGCGACCAGCATTG‑CGCCCTTCTTGTTG‑CTGAGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTAAGTGAGGCAAAAACAGGCAGGTGATAACAGTC‑GGCAAAAACCGACGTTAACGGTTCTGTGAAAAAACGCTTCCCCCAGTGGCGAGCGAAACGCTGTCGTTCCGACCGTTGTTCACCATATTGCacaccgggatgcacgacc                                                                                                                                                 >  2:433102/1‑234 (MQ=255)
                                                                                                             aCGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGNTCAGCATTTTGCa                                                                                                                                                                  >  2:244972/1‑233 (MQ=255)
                                                                                                               gTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCCTTGTTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGGTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGCGAAAACGCTGCACCCATTGGCGATCGGCACGCTGACGTTCCGCACGTTGTTCCGCATTTTGCAGCCCCGGATGCCCGCCTTCg                                                                                                                                              >  2:480593/1‑251 (MQ=255)
                                                                                                                 aaaccAGGCTGGCCGGCGAGAGAAGTCCCCTCC‑AGAATGGCGGCAAGCTTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCCCCCGGCGTGGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGAc                                                                                                                                            <  1:29771/247‑1 (MQ=255)
                                                                                                                    ccgggcgggACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAGGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATCCCAGTC‑GGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAAt                                                                                                                                         <  1:10599/244‑1 (MQ=255)
                                                                                                                                    gagaAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCCGCGCCACCAGCTCCCGGCGTTGCTCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACACATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCCGCATTTTGCAGCCCCGAGTGCCCGCCTTCGCCAATCACCCCACAAAGcccc                                                                                                                         >  2:169836/1‑251 (MQ=255)
                                                                                                                                            gCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑ATGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAAATACCCCACAAAGCCCCGCCAGccc                                                                                                                 >  1:191347/1‑251 (MQ=255)
                                                                                                                                                                        gcCATTATTGTTG‑GTGCGCAGCGCCACCAGATCCCGGCGTTGATAGTAATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTA‑GGCAAATCCCGCCGTAAACGGTGCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGAACATTGTTCAGCACATTGCAGCCCCGGATTCCCGCCTTAGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCCAgcccccctaacccccaccc                                                                                       >  2:79472/1‑235 (MQ=255)
                                                                                                                                                                            ttattGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGATGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGCTGCCCGCCTTCGACAATAAACCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTcc                                                                                   >  1:406347/1‑251 (MQ=255)
                                                                                                                                                                                 gttg‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCCCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTccacca                                                                               >  1:463955/1‑250 (MQ=255)
                                                                                                                                                                                          gcAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTAc                                                                     >  1:50834/1‑251 (MQ=255)
                                                                                                                                                                                                    caGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGAGACCAGTC‑GGCAAATACCGCCGTTAACGGATCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCAGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCAccagcc                                                           >  1:298787/1‑251 (MQ=255)
                                                                                                                                                                                                    caGCTACCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGCTCCCAGTC‑GGCAACTACCGCCGTTAAAGGTTCTGTGAGACAACGCTGCACCCATTGGCCATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATCCCCGCCCTCGACAATAACCACACAAAGCCCCGCCAGCCACTGGCAAGCCGCCATCATCGCCACCCGTCCACCAATCGA‑GTAccccccaagcc                                                           >  2:467534/1‑244 (MQ=255)
                                                                                                                                                                                                       cTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCCGCCCCTGGCAAGCCGCCATCCTCGCCACCCGTCCCCCAAGCGA‑GTACCCCCccagcc                                                           >  2:8296/1‑248 (MQ=255)
                                                                                                                                                                                                         cccGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGa                                                        >  1:229109/1‑249 (MQ=255)
                                                                                                                                                                                                                        cATTCAGTGAGGCAAAAACAGGCTGTTGATCCCAGTC‑GGCCACTACCGCCGTTACCGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGACGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAACCCCGCCATCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCCACCGATGT‑CCCCACCAGCACGAAGTCAAGGAtagtgga                                        >  2:59497/1‑244 (MQ=255)
                                                                                                                                                                                                                         aTTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGtaac                                      >  1:242542/1‑251 (MQ=255)
                                                                                                                                                                                                                           tgagtgagGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGCTCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCCCAAAGACCCCACAGCCCCTGGCAAGCCGCCATCATCGCCACCCGCCCACCCAGCGA‑GTACCCCCCCAGCCCGAAGTCAAGGATGTTGgaact                                     >  2:387967/1‑250 (MQ=255)
                                                                                                                                                                                                                            gagtgagACAAAAGGAGGTTGGTGATACCAGAC‑GGAAAACCCCGCCGGTAACGGTTCTTTGAGAAAACGCCGCCCCCAGTTGCGATCGGACCGCGGACGGTCCGCACGTTGTCCAGCATTTTGCAGCCCCGCATGCCCGCCTTCGACAATACCCCCAAAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGtaactaa                                   <  2:941/251‑1 (MQ=255)
                                                                                                                                                                                                                                    cAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCAc                                                                                         >  2:86693/1‑190 (MQ=255)
                                                                                                                                                                                                                                         aCAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACg                      >  1:10809/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGCCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGt                  >  1:390268/1‑250 (MQ=255)
                                                                                                                                                                                                                                                gTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCCTCATCGCCACCCGCCCACCACGCGA‑GGACCCCACCAGCCAGACGTCCAGGATGTTGTACCTACCCAAGGTTTTACGCAGTa                 >  2:126543/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        cAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGACCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGGCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAc       >  1:162169/1‑251 (MQ=255)
                                                                                                                                                                                                                                                              ggCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatcat  >  1:192978/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                         
GGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA‑TGTGCGGG‑CGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCC‑AGCATGGCGGCAAGCGTTG‑CGCCATTATTGTTG‑CTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTC‑GGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGA‑GTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCAT  >  NC_000913/2376910‑2377408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑