Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,244,212	0	C	T	33.3%	64.1 / 13.8	56	G101G (GGC→GGT)	yeiG	S‑formylglutathione hydrolase/S‑lactoylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base C (22/12);  new base T (0/17);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 3.66e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGC  >  NC_000913/2243966‑2244457                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                        cGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCtttt                                                                                                                                                                                                                                                     <  1:197569/251‑1 (MQ=255)      ccAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCt                                                                                                                                                                                                                                                 <  1:218911/250‑1 (MQ=255)      ccAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCGCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCtt                                                                                                                                                                                                                                                <  2:36647/251‑1 (MQ=255)           aCCTTAAACTGCCCGATGGCTTTCAGTATCTTTCTCCCTCCACCCCGTGATCACCCCCCGCCACCAGTGCT‑GTACTGGCTTGCCGGATTATCCTGCAATGACGAGAACTTCACCACCAAGGCTGGTGCCCATCGGGTAGCGGCGGAAC‑TGTGGATT‑GTACTGGTGATGCCAGACACCAGCGCGCGCGGCGAAAAGG‑TTG‑CCAA‑CTACGATGG‑CTACGATTTAGGCC‑AGGGCGGAGTCTTTTATCTTAATg                                                                                                                                                                                                                                            <  2:339035/250‑1 (MQ=255)                   cTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGGAC‑TGGGGGTT‑GTACTGGGGGTGCCAGACACCCGACCGCCCGGCGGACAGG‑GTG‑CCAAACG‑CGGTGG‑GTACCGTTTAGGGC‑AGGGCGCCGGGCTTTATCTTTATgtcccgcca                                                                                                                                                                                                                                    >  2:542757/1‑242 (MQ=255)                       ccGATGACGTTCATTATCTTTCCCCCTCCCCCGCGTGATCCCACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AAccgcc                                                                                                                                                                                                                             <  2:513150/251‑1 (MQ=255)                            gACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGGGATGCCAGACACCAGCCCGCGCGGCGAAAAAG‑TTG‑CCAA‑CGACGATGG‑CTACGGTTTAGGCC‑CGGGCGCAGGGTTTTATCTTTATGCC‑ACGC‑CACCGCCCTggg                                                                                                                                                                                                                        >  1:246663/1‑251 (MQ=255)                                            ctccctccACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGGAT‑GTACTGGTGATGCCAGGCACCAGCCCGCGCGGCGTAAAGG‑TTG‑CCAA‑AGACGATGG‑CCACGATTTAGGCC‑AGGGCGCAGGCGTTTATCTTAATGCC‑ACGC‑AACCGCCGCTGGCGACGCATTACCgggt                                                                                                                                                                                                        >  1:528406/1‑249 (MQ=255)                                               cctccACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTAATGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑GTG‑CCAA‑CGACGATGG‑CCACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGGGGGCGACGCATTACCGGATGt                                                                                                                                                                                                      >  1:414512/1‑250 (MQ=255)                                                ccccccctcgcgctcccaccccGCCACCAGTNCG‑GTAGGGGCGTTCGGGATAACCCGGCAGTGACGAGAACTTCCCCCCCAGGGGGGGTGCCCAGCGGGTGGGGGCGAAAC‑GGGGGATT‑GTACGGGTGACGCCAGCCCCCAGCCCGCGGGGCAAAAGGG‑TGG‑CCAA‑CGACGAGGG‑CTACGATTGAGGCC‑AGGGCGCGGGTTTTTATCTTA‑TGCCCACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTAt                                                                                                                                                                                                    <  2:249136/231‑1 (MQ=255)                                                cccccccccGTGCTCACACCCCGCCCCCAGTGCT‑GTACTGGCTTTCCGGATTAACCGGCAATGACGAGAACTCCCCCCCCAAGGGGGGGCCCCAGCGGGGAGCGGCGGAAC‑GGGGGTTT‑GTACTGGTGATGCCAGACCCCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑GGGGCGCAGGCTTTTATCTTAAGGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTAt                                                                                                                                                                                                    <  1:471041/243‑1 (MQ=255)                                                    aCCTCGTGATCACACTCCGCCACCAGTGCT‑GTAATGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGAGACCAGCGGGTAGCGGCGGAACCTGGGGATA‑G‑ACTGGTGGTGGCAGACACCAGCCCGCGCGGCGGAAAAG‑TTG‑CCAA‑CGACGGGGG‑CTCCGAATTAGGCC‑CGGGCGCAGGAGTTTATCTTAATGCA‑ACGC‑AACCGCCGTGGGCGGCGACTTAACGGAGGTATGAtt                                                                                                                                                                                                >  1:431311/1‑251 (MQ=255)                                                     ccTCTTGATCCCACTCCGCCACCAGTGCT‑GTAGTGGCTTTCCGGATTAACCTGCAAGGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GGACTGGTGATGCCAGACACCAGCCCGGGCGGCGAAAAGG‑TTC‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑GGGGCGCAGGTTTTTATTTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTa                                                                                                                                                                                               <  1:71103/251‑1 (MQ=255)                                                         gTGACCACACTCCCCCACCAGTGCT‑TTACTGGCTTTCCGGATAACCCTGAAATGAAGAGACCTCCACCACCAAGGCGGGTGCCCAGGGGGTAGCGGCGGAAG‑GGGGGTTT‑GTTCTGGTGATGCCGGACCCCAGCCCGCGCGGGTAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGTTTTAGGCC‑AGGGCGCAGGCTTTTATTTTAATCCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTg                                                                                                                                                                                           <  1:65370/251‑1 (MQ=255)                                                           gATCACCCCCCGCCCCCTGTGCT‑GTACTGGCTTTCCGGATTAACCTGAAAGGAGGAGACCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGGGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGT‑TTG‑CCAA‑CGACGATGG‑CTACGATTTGGGCC‑GGGGCGCAGGTTTTTATCTAAAGGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgcg                                                                                                                                                                                         <  1:120767/251‑1 (MQ=255)                                                           aaccacccTCCCCCACCAGTGCG‑TTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTCCACCCCCAGGGGGGGTCCCCAGCGGGTAGCGGCGGACC‑TGGGGATT‑GTACTGGTGATGCCAGACCCCAGCCCGCGGGGCGAAAAGG‑TGG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGTTTTTATCTTAATGCC‑ACCC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgc                                                                                                                                                                                          <  1:260882/243‑1 (MQ=255)                                                            cccccccacctccaccaGTGCTTGT‑CTGGTTTTCCGGATTACCCTGCAATGACGAGAACCTCACCACCAAGGCGGGGGCCCAGCGGGTAGCGGGGGAAG‑GGGGGATT‑GTTCTGGTGATTCCAGACACCCCCCCGGGCGGAGAAAGGT‑TTG‑CCAA‑CGACG‑TGGTCTTGGATTGGGGCG‑AGGGCGGGGTCTTTTATTTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGTATGTATGATTATCTgcgc                                                                                                                                                                                        <  2:450599/240‑1 (MQ=255)                                                                     cgcccccaggacT‑GTACTGGCTTTCCGGTTTACCCTGTAATGACGACCCCCCCCCCCCCAGGGCGGGTGCCCGTGGGGTGGGGGCGGACC‑GGGGAATT‑TTACTGGTAAGGCCAGCCCCCGCCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CCCCAATGT‑TTACGGTTTAGGCC‑GGGGCACAGGTTTTTTTGTTAATGCC‑GCCC‑ATCCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTa                                                                                                                                                                               <  1:270022/240‑1 (MQ=255)                                                                              gtgttggacgggCTTTCCGGATTATCCGGCAATGAGGAAAACTACCCCACCAGGGGGGGTGCCCAGCGGGTAGCGGCGGAAG‑GGGGTATT‑GTACGGGGGATGCAAGCCACAGGCCCGGGCGG‑GAAAAAG‑TTGGCCAA‑GGAGGGTGG‑CTAGGATTGAGGCA‑GGGGCGCAGGCTTTTATTTAAATGCA‑AAGA‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCt                                                                                                                                                                       <  1:471620/241‑1 (MQ=255)                                                                                              ccGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCCGACACCCGCCCGCGCGGCGAAAAGG‑GTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑CACCGCCGTGGGCGACGCATTACCGGGTGTATGGTTATCTTCGCGGTGAATTACCGGCGCTGGTTCAGGTGCAAttt                                                                                                                                                       >  1:423160/1‑251 (MQ=255)                                                                                                 gATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCCGCCCGCGCGGCGAAAAGG‑GTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AAACGCCGTGGGCGACGCATTACCGGATGGATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAAt                                                                                                                                                    >  1:498190/1‑251 (MQ=255)                                                                                                           cAATGACGAGACCTTCACCACCAAGGCGGGTGCCCAGCGGGGAGCCGCGGAAC‑TTGGGATT‑TTACTGGTGATGCCCCACACCACGCCGAGCGGCGCAAAGA‑CTG‑CCCA‑CGACGGTGG‑GTACGGTTTCAGCC‑AGGGGGCAGGCTTTTATCTTAATGCC‑CCGC‑AAACGCCGTGGGCGACGCATTAACCGAAGGATGATTATATGCGCGATGAATTACCGGCGCCGGTTCatgcgcagcttaatgtgcacggac                                                                                                                                          >  2:35262/1‑228 (MQ=255)                                                                                                            aaTGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCACACCCCAGCCCGCGCGGCGCAAAGG‑ATG‑CCAC‑CGACGATGG‑CCACGATTTACAGC‑ATGGGGCAGGGTTTTATCTTAATGCC‑CCCC‑AACCGCCCTGGGCGTCGCATTGCCGGATGTATGATTATCTGCGGGGTGAATTAACGGCGCTGGTTCAGTCGCACTTTTATGTCAGCGAccc                                                                                                                                         >  1:180217/1‑250 (MQ=255)                                                                                                            aaTGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGGGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCg                                                                                                                                         >  1:74572/1‑251 (MQ=255)                                                                                                                         tCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGAAT‑TTACTGGGGATGCCAGACAACCGACCGCGCGGCGAAAAGG‑GTG‑CCAA‑CGACGATGG‑CCACGATTTAGGCC‑CGGGGGCAGGGTTTTATCTTAATGGC‑CCGC‑AACCGCCGTGGGGGACCCAGTAACGGATGGTTGGTTTTTTGCGCGGTGAATTACCGGCCCTGGGTCCGGCGCAATTTTATTGCAGCGGCCGCTGCGCCATTAgg                                                                                                                            >  2:133243/1‑250 (MQ=255)                                                                                                                            ccaccaAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCCGACACCAGCCCGCGCGGCGAAAAGG‑GTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGGTGTATGGTTATCTTCGCGGTGAATTACCGGGGCTGGTTTAGACGCAATTTAATGTCACCGACCGCTGCGCCCTTAGCggg                                                                                                                         >  1:539380/1‑250 (MQ=255)                                                                                                                                 aagccggttGCCCAGCGGGTAGTGGCGGAAC‑GGGGAATT‑GTTCTTGTGATCCCACACCCCCGCCCGCGCG‑CGAAAAGGGTTG‑CCAC‑CTCCGATGG‑CTACTTTTTAGGCA‑AGGCCGCAGTTTTTTTTCTTA‑TGCC‑CCCCCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGGCAGCGTCCGCTGCGCCATTAGCGGTCACTc                                                                                                                    <  1:209965/243‑1 (MQ=255)                                                                                                                                      gggTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCCATgg                                                                                                               >  1:155820/1‑251 (MQ=255)                                                                                                                                            ccAGCGGGGAGCGGGGGAAG‑TGGGGGTT‑GCCCGGGTGATGCCAGACCCAACCCCGCGCGGCAAAAGGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑GGGGCGCAGGTTTTTATCTTAATGCC‑CCGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTc                                                                                                         <  1:370683/251‑1 (MQ=255)                                                                                                                                              aGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGGGGTCAc                                                                                                       >  1:337456/1‑251 (MQ=255)                                                                                                                                                     aacggcggAAC‑TGGGGGTT‑TGACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑TCAA‑CGACGAGGG‑CTACGATTTAGGCC‑AGGGGGCAGGCTTTTCTCTTAAAGCC‑ACGC‑CAACGCCGTGGGCGACGCCTTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCCGGCGCAATTTTATGTCAGCGACCGCTTCGCCCTTCGCGGTCACTCAATGGGGGGTCACGGcgcgg                                                                                                >  2:388554/3‑250 (MQ=255)                                                                                                                                                        ggggcgtaatGGGGATTTGCACTGGTGATGCAAGACCCCGGCCCGCGGGGCAAAAAGG‑TTG‑CCAA‑CGAGGGTGG‑CTAGGATTTAGGCC‑AGGGCGGAGGTTTTAATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCAATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCt                                                                                               <  1:354884/242‑1 (MQ=255)                                                                                                                                                         gcggAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGAt                                                                                            >  2:412992/1‑251 (MQ=255)                                                                                                                                                             gaccgggggATT‑GGACTGGGGATGCCAGACACCAGCCCGCGGGGCAAAAAGG‑TTG‑CCAA‑CGAGGATGG‑CTACGATTTGGGCC‑AGGGCGCAGGTTTTAATCTAAATGCC‑ACGA‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTAt                                                                                         <  1:12265/246‑1 (MQ=255)                                                                                                                                                               c‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGCTTATGgc                                                                                      >  1:274630/1‑251 (MQ=255)                                                                                                                                                                               ggTGAGGCCACACACCACCCCGGGCGGGAAAAAGG‑TGC‑CCAA‑CTCCGATGG‑CTACATTTTAGCCC‑GGGCCGAAGTTTTTTATCTTAATCCC‑CCGC‑ACCCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCgg                                                                        <  1:479366/251‑1 (MQ=255)                                                                                                                                                                                        aGACCCCACCCCGCGGGGCGAAAAGG‑TTG‑CCAA‑GGACGATGG‑CTACG‑TTTAGGCCCGGGGCGCAGGTTTTTATCTAAAGGCC‑CCGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATaca                                                               <  1:524779/251‑1 (MQ=255)                                                                                                                                                                                           cacCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACAcc                                                             >  1:183143/1‑250 (MQ=255)                                                                                                                                                                                            cccgccccGCGCGGGGAAAAGT‑TTG‑CCAA‑CGGCGAGGG‑CTACGTTTGAGGCC‑GGGGCGCAGTCTTTTTTCTTAATGCC‑CCGC‑ACCCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAg                                                           <  2:344181/246‑1 (MQ=255)                                                                                                                                                                                                  ccggggggagAAAAGT‑TGG‑CCAA‑CGACGAGGT‑CTAGGATTTGGGCA‑GGGGCGGAGTTTTTTATCTTAAGGCC‑CCGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTc                                                     <  2:498190/242‑1 (MQ=255)                                                                                                                                                                                                   cgcgcgGGGAAAAGG‑TGG‑CAAA‑CGCTGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGTTTTTATCTTAATCCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCg                                                    <  1:169562/251‑1 (MQ=255)                                                                                                                                                                                                           gAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTgc                                            >  2:455674/1‑251 (MQ=255)                                                                                                                                                                                                             aaaGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTgcgc                                          >  1:394085/1‑251 (MQ=255)                                                                                                                                                                                                               aagtttG‑CCAA‑CTACAGTGG‑CGACGATTTGGGCC‑AGGGCGCAGGTTTTAAGCTAAATCCC‑CCCA‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGCTTCGGCCTTTGCGcc                                         <  1:478377/247‑1 (MQ=255)                                                                                                                                                                                                                 g‑TTG‑CCAA‑CGACGATGG‑CTACGATTTCGGCC‑AGGGCGCAGGCTTTTATCTTAAGGCC‑ACGC‑AACCGCCGGGGGCGACGCACTTCCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCACTTTAATGTCAGCGACAGCTGCGCCACTAGCGGTCACTCAATGGGTGGTCACGGCGCGCTGTTTAGGGCGCTGAAAAATCCGGGTTAATAAACCAGCGGTTCGGCCTTTGCGCCAAt                                      >  2:52843/1‑251 (MQ=255)                                                                                                                                                                                                                          a‑cgacgGTGG‑CTACGATTTAGGCC‑AGGGCGCAGGTTTTTATCTTAATGCC‑CCGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAAt                               <  1:147436/251‑1 (MQ=255)                                                                                                                                                                                                                             gacgaTGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑CACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCCTTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATcc                             >  1:268437/1‑251 (MQ=255)                                                                                                                                                                                                                                 gagggcaaagctttAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGGATGATTATCTGCGCGCTGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGAGACCGCTGCGCCAATAGCGGTCCCTCATTGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAACAACCGGGTAAAGACACCAACGTTTCGGCCTTTGCGCCACTtggacattcgtg                          >  2:472688/12‑241 (MQ=255)                                                                                                                                                                                                                                       tACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGt                    >  1:322730/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTcc                  >  1:445436/1‑251 (MQ=255)                                                                                                                                                                                                                                          gATTTAGGCC‑AGGGCGCAGGTTTTTATCTTAATGCC‑CCGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTccc                 <  1:16993/251‑1 (MQ=255)                                                                                                                                                                                                                                            tttAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGt               >  1:471654/1‑251 (MQ=255)                                                                                                                                                                                                                                             ttAGGCG‑AGGGCGCAGGTTTTTATCTTAATGCC‑ACGC‑ACCCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGt               <  1:113274/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTgggg           >  1:422961/1‑250 (MQ=255)                                                                                                                                                                                                                                                     aGGGCGCGGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATc       <  1:415202/251‑1 (MQ=255)                                                                                                                                                                                                                                                          gcAGTCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGc  <  1:413107/251‑1 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                        CGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCT‑GTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAC‑TGGGGATT‑GTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGG‑TTG‑CCAA‑CGACGATGG‑CTACGATTTAGGCC‑AGGGCGCAGGCTTTTATCTTAATGCC‑ACGC‑AACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGC  >  NC_000913/2243966‑2244457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑