| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,335,717 | 0 | C | T | 22.6% | 117.1 / 10.4 | 62 | intergenic (+50/‑114) | ymiC/acnA | protein YmiC/aconitate hydratase 1 |
| Reads supporting (aligned to +/- strand): ref base C (17/31); new base T (14/0); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGT > NC_000913/1335469‑1335961 | tCTTAGTACCCATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTGAAGGGTGTAGTCTATGATCAACACAAATATGAAATATGGGTCCTGGATGGGCGCTTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTCCTTTGCCACAAGT‑TTtct < 2:448206/250‑1 (MQ=255) ccATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTTCTTTGCCACAAGG‑TTTTTCCTCTTTTAt > 1:312165/1‑251 (MQ=255) tttAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAAt < 1:197424/250‑1 (MQ=255) ttAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTTG‑CATTTGCTTTGCCACAAGG‑GTTCTCCTCTTTTATCCAttt > 1:479879/1‑251 (MQ=255) tAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGGCATTTCGTTTTCCTCAAGG‑TTTCTCCTCTTTTATCAATTTg > 1:504263/1‑251 (MQ=255) attaatttaatggCTTTTAATACACCGCAGCAAAAACAGCTTGAGTTATATCAACACAAAAGAATAAACGTTAAGGTTGTAGCCTATGATCAACACAAATAGGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGCCGATGCTCTTCTGGTACGATCTCCGCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTTTCATTTGCTTGGCCACAATG‑TTTCTCATCTTTTATCAATTTggg < 2:314189/240‑1 (MQ=255) gAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTTGATCATTACTCACTGATCCTTGGCCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGgtt < 1:504562/251‑1 (MQ=255) aaGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑c < 1:67590/251‑1 (MQ=255) ttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTTCCACAAGG‑GTTCTCCTCTTTTATCAATTTGGGGTGTTAT‑CAAATCGtt > 1:52939/1‑251 (MQ=255) aaTAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCtttt < 2:344943/209‑1 (MQ=255) aaTAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAAG‑GTTCTCCTCtttt > 1:344943/1‑209 (MQ=255) aTAACAGCTTGAGTTATCTTAAAACAAAATAATAAACGTTAACGGTGTAGCCTATGATCAACACAAATAGGAAATATTTGTCCTGGATGGGCGCGTTTTCTCTGTCGATTCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TGTCTCCTCTTTTATCAATTTGGGTTGGTAT‑CAAATCGTTACGCTATGTTTGTGtt < 2:525308/251‑1 (MQ=255) tAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTTCCACAAGG‑TTTTTCCTCTTTTATCAATTTTGGTTGTTTT‑CAAATCGTTTCGCGATGTTTGTGttt > 1:330613/1‑250 (MQ=255) aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATc < 1:534523/251‑1 (MQ=255) aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATc < 2:330613/251‑1 (MQ=255) aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATc < 1:401177/251‑1 (MQ=255) aCAGCGTGAGTTATCACAACACAAAATAATAACCGTGAAGGGTGTAGCCTATGATCAACACAAATATTAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGTGCCGAACTCCTCTGGATCATTACTCACTGAGCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGTTTGTTAT‑CAAAGCGTTACGCGATGGTTGTGTTATc < 2:145225/251‑1 (MQ=255) cAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GCGTCATTTGTTTTTCCCCAAGG‑GTTTTCCTCTTTTATCACTTTGGGTTGTTTT‑CAAATCGTTACGCGATTTTTTTGTTATCt > 2:424114/1‑251 (MQ=255) aatcTTGAGTTATATCAAAACAAAATAATAACCGTTTAGGGTGTAGCCTATGATCAACACAAATATAAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTGCTGGGCCGAACTCCTCGGGATCATTACTCACTGATCCTTGTCCCCGCTGCGGCGGTG‑TTGTCATTTGCTTTGCCACAAGG‑CTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCt < 2:336320/248‑1 (MQ=255) acacAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTTATTTTCTTTTTCCCAAGG‑GTTTTCCTCTTTTATCAATTTGTGTTGTTTT‑CACAACGTTACGCGNTGTTTGTGTTTTTTTTAattttttacctgt > 2:257064/1‑239 (MQ=255) ataataACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTTTCCTCTTTTTTCAATTTGGGTTGTTAT‑CAAATCGTTTTGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑Tc > 1:240790/1‑251 (MQ=255) ataaCCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCTCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTGGAATATTCACCCTGAAGAGAA‑TCAgg < 1:167207/251‑1 (MQ=255) ataaCCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAgg < 2:479879/251‑1 (MQ=255) gTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTTCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGGTGTTTT‑CAAAACGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑Cg > 1:344388/1‑251 (MQ=255) tgtAGCCTATGATCAACACAAATATGAAGTATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGACCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCCTTTTCTTTTCCACAACG‑GTTCTTCTCTTTTTTCAATTTTGGCTGTTTT‑TAAAACGCGACTCAGTGTTTGCTTTTTCTTTAATATTTACCCTGAAGAGAAACCAGGG‑TT‑CGCCACCCTg > 2:312379/1‑251 (MQ=255) aGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCCTTTGCTTTGCCCCAAGG‑TTTCTCCTCTTTTTTCAATTTTGGGTGTTAT‑CAAATCGGTACGCGATGTTTTTGTTATCTTTAATATTTACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTcc > 1:161163/1‑250 (MQ=255) aTGATCAACACAACTATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGGAATTTGTGTTGCCACAAAG‑TTTTTCCTCTTTTATCAATTTGGGTTGTTTTTCAAA‑CGTTACGCGGTGTTTTTTTTTTCTTTTATATTTACCCTGAAGAGAA‑TCAGGGATT‑TGCCACCCTGTCAttta > 2:78194/1‑248 (MQ=255) aTGATCAACACAAATATGATATATTTGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTTTTCTGGGCCGAACTCCTCTGGATCATTACTCACTTATCCTTGACCCCTCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAATG‑TTTCTCCTCTTTTATCAATTTTGGTTGGTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCAttt < 2:68412/249‑2 (MQ=255) aTCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTGCCACACAG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑TAAAACGTTTCGCGATGTTTGTGTTTTCTTTAATATTCACCCTGAAGAGAA‑TTAGGGCTT‑CGCAACCCTGTCATTAagg > 1:499739/1‑249 (MQ=255) tCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTGCCACCAGG‑TTTCTCCTCCTTTTTCAATTTTGGTTGTTTT‑CAAATCGTTACGCCGTGTTTGTGTTATTTTTTATATTTACCCTGAAGAGAAATC‑GGGCTT‑CGCAACCCTGTCATTattgagg > 2:501234/1‑245 (MQ=255) cAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACGCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAaggagg < 1:47246/250‑1 (MQ=255) cacaAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCATGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCt < 1:199021/251‑1 (MQ=255) cacaAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTTATTTGCTTTTCCACAAGG‑TTTTTCCTCTTTTATCAATTTTGGTTGTTTT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAAGGAA‑TCAGGGCTTTCCCAACC‑TGTCATTAAGGAGGAGCt > 1:264057/1‑251 (MQ=255) aaTATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTTCTTTTCCACAAGG‑TTTCTCCTCTTTTTTCAATTTTGGGTGTTAT‑TAAATCTGTACGCGGTGTTTGTGTTATCTTTAATATTTACCCTGTAGAGAA‑TCAGGGCTT‑CGCAACCCTTTCATTAAGGAGGAGCTATgt > 1:398930/1‑251 (MQ=255) atGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATgtcgt < 1:4304/251‑1 (MQ=255) atGAAATATTGGTACTGGATTGGCGCGTTTTCTCTGTCGATGCTCTTCTTGGCCGAACTCCTCTGTATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTTCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATgtcgt < 1:270244/251‑1 (MQ=255) atatTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTTCCACAAGGGTTTTTCC‑CTTTTATCAATTTGGGGTGTTAT‑CCAATCGTTACGCGATGTTTGTGTTTTCTTTTATATTTACCCTGAAGGGAA‑TCAGGGCGT‑CGCAACCCCGTCCTTACAGAGGCGCAATGTCGTCaaac > 1:387728/1‑249 (MQ=255) atatTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTGCCCCCAGG‑GTTTTCCTCTTTTTTCAATTTGGGGTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTTACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAAcc > 1:3898/1‑251 (MQ=255) tatTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCCTTTGCTTTTCCACAAGG‑TTTTTCCTCTTTTTTCAATTTGGGTTGGTAT‑CAAAACGTTACGCGATGTTTGTGTTTTTTTTAATATTCACCCCGTAGAGAA‑TCAGGGGTT‑TGCAACCCTTTCATTTAGGAGGAGCTATGTCGTCAAccc > 2:526164/1‑251 (MQ=255) ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTTCCACAAAG‑TTTTTCCTCTTTTATCAATTTGGGGTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTTTTTTTAATATTCAACCTGAAAGGAA‑TCAGGGCTT‑CCCCACCCTGTCATTTAGGGGGGGGTTTGTCGTCaaacccacg > 1:308670/1‑244 (MQ=255) ggATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAg < 1:526164/250‑1 (MQ=255) gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAAt < 2:277217/126‑1 (MQ=255) gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCCTTTTCTTTGCCACAAGG‑GTTCTCCTCTTTTATCAAt > 1:277217/1‑126 (MQ=255) gTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTTTCATTTGCCTTTCCACCAAG‑TTTCTCCTCTTTTATCAATTTGGGTGTTTAT‑CAAATCGTTACGCGCTGTTTGTGTTATCTTTAATATTTACCCTGACGAGAA‑TCAGGGCTT‑CGCACCCCCGGCATTAAGGAGGAGCTATGTCGGCAAccccccgaagagccagcaaggcc > 2:27988/1‑231 (MQ=255) gTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTTCCACCAGG‑TTTCTCCTCTTTTATCAAATTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACAAGAAGCCAGTCAGGa > 1:315001/1‑250 (MQ=255) gTCGATGCTCTTCTGGGCCGAACTCCTCTGGAACATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTGGGGTTGTTAT‑CAAATCGTTACGCGATGGTTGTGTTATCTGTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCTTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAg < 1:193400/251‑1 (MQ=255) gATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAACTTGGGGTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCCGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAAGACAACTTGCAGGcc > 1:76434/1‑251 (MQ=255) gCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCGTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTAGCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGGGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCaaa < 1:78194/251‑1 (MQ=255) tcttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTa < 1:485543/141‑1 (MQ=255) tcttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCCTTTTCTTTTCCACCAGG‑TTTTTCCTCTTTTTTTAATTTTGGGTGTTAT‑TAAATCGTTACGCGGTGTTTTTGttt > 2:485543/1‑140 (MQ=255) cttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAg < 1:225923/249‑1 (MQ=255) tctGGGCCGAACTCCTCTGGATCATTACCCACTTATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTAACAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa < 1:363974/250‑1 (MQ=255) gAACTCCTCTGGATCATTACTCACTGATCCTNGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc < 2:264057/251‑1 (MQ=255) ggATCATTACTCACTGGTCCTTGACCCCNCTGCGGCTGGG‑TTGTCAGTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGGTTGTGTTATCGTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCActacta < 2:266829/248‑1 (MQ=255) gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCCTTTGCTTTTCCACAAGG‑TTTTTCCTCTTTTTTAAATTTTGGATGTTTT‑CAAATCGTTACGCCATGATTTTGTTATTTTTTATATTCTCCCCGAAGGGAA‑TCAGGGCAT‑CGCAACCCTGTCATTAAGGAGGAGCTATTTCGTCAAACCTAAGTGAAGCCAGGAAGGGCACGATGCAGGACAACGATAAAACTTACCACTACTAAAGc > 1:405173/1‑251 (MQ=255) aTCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑GTGTCATTTTCTTTTCCACAAGG‑GTTTTCCTCTTTTATCAATTTGGGGTGTTTT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTTCCCCTGAAGAGAA‑TCAGGGCTT‑TGCAACCCTGCCATTTAGGAGGAGCTATGTAGTCAAACCTACGAGAAGCCAGTAAGGACAAGTTGCAGGCCCAAGATAAAACTTAACACCACTACAGcc > 1:101903/1‑251 (MQ=255) cACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGGTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCGT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACGTACCACTACTACAGCCTGCCGCTtg < 1:173857/251‑1 (MQ=255) gATCCTTGCCCCCGCTGCGGCGGGG‑TTGTCATTTGTTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGATATGTCGTCAACCCTACGAGAAGCCAGTAAGGATACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgct < 1:484085/249‑1 (MQ=255) gATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCGGCCGCTtgctgc < 1:110560/251‑1 (MQ=255) gATCCTTGACCCCGCTGCGGCGGGG‑GTTTCCTTTTCTTTGCCCCAAGG‑TTTCTCCTCTTTTTTCAATTTTGGTTGTTAT‑CAAAATGTTACGCGCTGTTTGTGTTTTCTTTAATATTCTCCCCGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACACTACGAGAAACCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAACCTGCCGCTtgctgc > 1:383213/1‑251 (MQ=255) gTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCt > 1:450917/1‑251 (MQ=255) tCATTTGCTTTGCCACAAGG‑TTTCTCCTCTNTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCt < 2:240790/250‑1 (MQ=255) aGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGCCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGt > 2:497131/1‑251 (MQ=255) | TCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGG‑TTGTCATTTGCTTTGCCACAAGG‑TTTCTCCTCTTTTATCAATTTGGGTTGTTAT‑CAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAA‑TCAGGGCTT‑CGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGT > NC_000913/1335469‑1335961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |