| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 884,184 | 0 | A | G | 36.2% | 65.7 / 12.1 | 59 | E171G (GAG→GGG) | mdfA | multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base A (20/17); new base G (17/4); total (38/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGTGGTTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAG > NC_000913/883939‑884425 | ggtgtggtTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGAAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATAAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTTGGCGCGGCG‑GGG‑GT‑CCATGTGCTGCCCTggg‑gggggg > 1:542926/1‑250 (MQ=255) gtgtggtTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGAt < 1:440137/251‑1 (MQ=255) gtgtggtTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGAt < 1:439838/251‑1 (MQ=255) ggtTTATCGTCAACTGTCTGGCTATATGTCTTGCGTAAAACATGGAGCCATTCACCCTTTTGCGCTGCTTGCAGGGCATATGCCTCTGTTTCACTTTCGCTGTGGGATACTCCGCAATTCAGGCATCCTTCGAAGGGGCGTTTTGTTTCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTCAGTTG‑TCCTCTGGTGGGCGCGGCG‑TTT‑AT‑CTATGTGCTGACCGGGG‑AGGGGAtgtt < 2:497521/250‑1 (MQ=255) tATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGAtgtttgtt < 1:345232/250‑1 (MQ=255) cACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTTTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTtttgtttg < 1:282720/251‑1 (MQ=255) cTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATCGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑GT‑CCCTGTGCTGCCCTGGG‑AGGGGATGGTTGTTTTGTTTGCCg > 1:489359/1‑251 (MQ=255) ggCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑TGG‑GT‑CCATGTGCTGCCCTGGG‑AGGGGGTGTTTGGTTTGTTTGCCGCATTgg > 1:506385/1‑251 (MQ=255) gCAATATTGCTGGCGCACAACATTGAACCATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTG‑ATAAAAGGTCACCCCGCTGGTTGCGCAAGGGGCGCTTGTTGTCCCGCT‑ACTTGGGTCGCTGGGTGGCGACGCG‑GGG‑AT‑CCATGGGCTGCCCTGGN‑GGGGGGGGGTTGTTTTGGTTATCGCATTggg > 2:248893/1‑250 (MQ=255) gCAATATTGATGGCTCAAAACTTTGAATAATTCTCCCTGTTTCGCTGTTTGCAGGGCATAAGCATCTGTTTCATTGTCGCTTTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTTGTACGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGTCCCGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGc < 2:517248/251‑1 (MQ=255) atTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGGGGGGGCGGCG‑GGG‑AT‑CCCTGTGCTGCCCTGGG‑AGGGGGTGTTTGTTTTGTTTGCCGCATTGGCAggg > 1:89163/1‑248 (MQ=255) atTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑TGG‑GT‑ACATGTGGTGCCCTGGG‑GGGGGGTGGTTGTTTTGGTTTCCGCAtttgccgcgg > 2:433401/1‑243 (MQ=255) gCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGAtct < 1:311767/251‑1 (MQ=255) cAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCttttt < 1:190788/251‑1 (MQ=255) aaCATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGGGCGGCG‑TGG‑GT‑CCATGGGGTGCCCTGGG‑CGGGGGTGTTTGTTTTTNTTTCCCCATTGGAGGCGGGCTCCtttttacg > 2:256385/1‑248 (MQ=255) cATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGGATCAA‑GATCACCGCGCTGATGGCGAAAGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGATGTTTGGTTTTTTTGCCGCATTGGCAGCGATCTTCTTTTTC‑GGTc > 1:15016/1‑251 (MQ=255) cATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGAGCTGTGGGCTACGCCGCACTTCAGGAATCCTTCGAAGAGCCTGTTTTTCTAAA‑GATCCCCGCGCTCATGGCTGACGAGGCGATGATTGCTCCGCT‑TCTTGGTCCGCTGGATGGCGCGGCGGCGG‑GT‑C‑ATGTCCTGCCCGGGG‑AGGAGATGTTTGGTTTGGTTGCCGCACTGGCAGCGGACTTCTTTTTccgac > 1:257757/1‑247 (MQ=255) aaTTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGGTGTTTGTTTTGTTTTCCGCATTGGCAGCGATCTCCTTTTTA‑GGTCTGTAACg > 1:324845/1‑250 (MQ=255) tCACCCTGTTGCGCTTCTTGCAGGGCATAATCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTGTTC‑GGTCTGCAACGAGc < 2:68596/250‑1 (MQ=255) cACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGCTGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑TGG‑AT‑CCCTGTGCTGCCCTGGG‑AGGGGGTGTTTGTTTTGTTTGCCGCATTGGCCGCGCTCTCCTTTTTC‑GGTCTGCAACGGGccc > 2:174741/1‑250 (MQ=255) cACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCAACGCGCTGATGGCGGACGTGGCGCTGGTTGGTCCGCT‑ACTTGGTCCGCCGGTGGGCGCGGCG‑TGG‑GT‑CCATGTGTTGCCCTGGG‑AGGGGGTGGTTGTTTTGTTTGGCCCCTTGGGAGCGATTTCCttttttgggttgcaacaggcc > 2:376736/1‑234 (MQ=255) gTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATAACCGCGCTTATGGCGCACGTGGCGCGGATTGCTCTGAT‑ACCTGGGTCGCTGGGGGGCGCGGCG‑TGG‑GTACCA‑GTGGTGGCCTGGG‑AGGGGGTATTTGTTTTGTTTGCCGCATTGGCCGCGAGCTCCTTTTTC‑GGCCTGCAAcaaggcctgccgg > 2:439376/1‑239 (MQ=255) gcgcTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGGTGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGaa > 1:120479/1‑250 (MQ=255) ttcttGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTCTCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGATGTTTGTTTTGTTTGCCGCGTTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGc > 1:329289/1‑251 (MQ=255) tcttGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGGTGTTTGGTTTGTTTGCCGCATTGGCAGCGAACTCCTTTTTC‑GGTCTGCAACGAGCCAAGCCTGAAACCGcc > 1:136436/1‑251 (MQ=255) cAGGGCATCAGACTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCCA‑GAACACCGCGCTGGTGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTACGCTGGTGGGCGCGGGG‑CGG‑GT‑CCATGGGCTGCCCTGGG‑GGGGGGTGTTTGTTTTGTTTGGCGCATTGGCCGCGCTCTCCTTTTTC‑GGTTTGCCACGGGCCCTGCCTGCAACCGccccccg > 2:305173/1‑246 (MQ=255) cAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCAcgcg > 1:78049/1‑251 (MQ=255) gggtcATAAGCCTCTGTTTAATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTGCGAAGAGGCGGTTTGTAGCAA‑GATCCCCGCGCGGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCGGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCACCGAGCCATGCCTGAAACCGCCACGCGt < 1:437627/247‑1 (MQ=255) aGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTTTATCAA‑GATCACCGCCCTGATGGCGAACGGGGCGCTGGTTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGGGGCG‑GGG‑AC‑CCAAGTTCTGGCCTGGG‑GGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTT‑GGTCTTCAACTAGCCCTGCCTTAAACCGCCACCCGt > 2:224457/1‑251 (MQ=255) gggCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCCA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑GT‑CCATGTGCTGCCCTGGG‑GGGGGATGTTTGTTTTGTTTGCCGCAATGGCCGGGGTCTCCCTTTTC‑TGGCTGCAACGAGCCCTGCCTGAAACCGCCACGCGtg > 2:458608/1‑250 (MQ=255) cATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATAAA‑ACTCCACGCGCTGAAGGCGAAAGTGGCGCCGAGTGCCCCGCT‑ACTTTGGCCGCCGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGCTGTTTGTTTTGTGTGCCGCATTTGCAGCGTTCTTCTTTTTC‑GTTCTGCAACGACCCATggcctataccgcatcgctaaacg > 2:22947/1‑229 (MQ=255) gTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGCTTGTATCAA‑GATCACCGCGCTGCTCGCGAACGCGGCGCTGTTTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑CGG‑AT‑GCCTGCGCTGCCCCGGG‑GGGGGATGTTTGGTTTGTTTGCCGCAATGGTAGCGATCTCCTTTTTT‑GGACTTCAACGCGCCCTGCCTGAAACCGCCACGCGGATAGGGGAGAAAccg > 1:127898/1‑249 (MQ=255) ttCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGAAGGCGAACGTGGCGCTGATTGTTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑GGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGGTGTTTGTTTTGTTTGCCGCATTGGCAGCGGTCTCCTTTTTT‑GGTCTGGAACGAGCCATGCCCGAAACCGCCACGCGGAAAGGCgggacaatggc > 1:502186/1‑241 (MQ=255) aTTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑TGG‑AT‑CCCTGTGCTGCCCTGGG‑GGGGGGTGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCCTGCCTGAANCCGCCCCGCGTATTGGCGCGAAACTGTccct > 2:235225/1‑248 (MQ=255) gcTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGCTGGCGAACGTGGCGCTGATTGGTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGGCCTGGG‑AGGGGGAGGTTGTTTTGTTTTCCGCCTTGGGCGGGGTCTCCTTTTTT‑GGTCTGCAAAGAGGCATGCCTGACACCGCCACGCGGATAAGCGAGGAAATGGCACTGaaaa > 1:52342/1‑249 (MQ=255) cgcAATTCAGGAATCCTTCGAAGAGGCGGTTTTTATCAA‑GATCACCGCGCTGATGGCGAACGGGGCGCTGATTGCTCCGCT‑ACTTTGTCCGCTGGTGGGCGCGGCG‑GGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGATGTTTGTTTTGTTTGCCCCATTGGCAGCGATATCCTTTTTC‑GGTCTGCAACGAGCCCTGCCTGAAACCGCCCCGCGTATAGGCGGGAAACTGTCACTGAAAGAAATCGGTCGGGAcc > 2:45434/1‑250 (MQ=255) gcAATTCAGGAATCCTTCGAAGAGGCGGATTGTATCAA‑GATCACCGCGCTGATTGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGGGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑GGGGGATGTGTGGTTTGTTTGCCGCATTGGCAGCGGTCTCCTTTTTC‑GGTCTGCAACTAGCCATGCCTGAAACCGCCACGCGTATATGCGAGAAACTGTCACTGTAAGAAATCTGTCGTGACt > 1:391779/1‑250 (MQ=255) aaTTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑AATCACCGCGCTGATTGCGAACGTGGCGCTGGTTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCCTGTGCTGCCCTGGG‑GGGGGATGTTTTTTTTGTTTGCCGCCTTGGTCGCGGTCTCCTTTTTT‑GGTCTGGCACGAGCCCTGCCTGAAACCGCCACGCGTATAGGCGGGAAACTGTCCCTGTAAGAACCCGGTCGTGGCtat > 2:220550/1‑250 (MQ=255) cAGGAATCCTTCGAAGAGGCGGCTTGTAACAA‑AATCACCGCGCTGATGGGGAACGTGGCGCTGGTTGCTCCGCT‑ACTTTGTACGCTGGTGGGCGCGGCG‑TGG‑AT‑CCCTGTGCTGCCCTGGG‑AGGGGATGGTTGTTTTGGTTGCCGCCTTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGACACCGCCACGCGTATAGGCGCGAAACTGTCACTGAAAGAACCCGGTCGTGACTATAAGCt > 2:354644/1‑251 (MQ=255) ggAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCCAACGGGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑TCATGTGCTGCCCTGGG‑GGGGGAGGGTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGGCTGCAACGAGCCACGCCTGTAAACGGCACGCGTATTGGGGGGGAACTGTCACTGGAAGAACTCGGGGGTGACGATAAGcggg > 2:97144/1‑248 (MQ=255) ccgttctgTTTGTACCAA‑GATCCCCGCGCTGNGGGCGAACGTGGCCCTGATTGCTCCGTT‑CCTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AC‑CCAGGTGCTGCCCTGGG‑GGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACg < 2:239430/244‑1 (MQ=255) ggCGTTTTGTATAAA‑GATCCCCGCTCTGATGGCGACCGGGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑GGG‑AT‑CCATGTGTGGCCCTGGG‑AGGGGATGTTTTTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCACCGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGcc < 1:363697/251‑1 (MQ=255) gTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACg > 1:239430/1‑244 (MQ=255) tgtacaaa‑GAACACCGCGCTGAGGGGGACCGTGGCGCTGATTGCCCCGCT‑CCGTGGTCCGCTGGTGGGCGGGGCG‑TGG‑AT‑CCATGTGCGGCCCGGGG‑AGGGGGTGTTTGTTTTGTTTGCCGCGTGGGCGGCGTTCTCCTTTTTC‑GGTCGGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTtg < 2:138112/244‑1 (MQ=255) tCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCg > 1:264078/1‑251 (MQ=255) aTCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGACACCGCCACGCGTATAGGCGAGCAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCggaggg > 1:39710/1‑247 (MQ=255) aTCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCgggggg > 2:195881/1‑250 (MQ=255) gcgcTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGCCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCggggggggcggg > 1:517581/1‑244 (MQ=255) cgcTGATGGAGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGCTGGGCGCGGCG‑TGG‑AT‑CCATGTGATGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGTCTATAAGCTGGTGCTGTAGAACGGGCGCTTTGTTGCggggggggggggg > 1:350542/1‑243 (MQ=255) gAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCgggggggcgggggcggggggtgg > 1:139741/1‑233 (MQ=255) aggTGGCGCTGATTGCTCCGCT‑TCTGGGCCCGCTGGGGGGCGGGGCG‑TGG‑AT‑CCATGTGCTCCCCTGGG‑GGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCACCGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAGCGGCCGCTTTGTGGCGGTGGCGCTGGCGCTGGGATTCGtt < 1:435563/249‑1 (MQ=255) gTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCgggggggccggggccgggggtggtt > 1:326762/1‑229 (MQ=255) ggggcTGATTGCTCAGCT‑ACTTGGTCGGTTGGTGGGCGCGG‑G‑TGGGAT‑CCTTGTGCTGCCCGGGG‑GGGGGATGTTTGTTTTTTTTGCCGCATTGGGAGCTATCGCCTTTTTC‑GGTCGGCAACGAGCCATGCATGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTc < 1:495331/248‑1 (MQ=255) gCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCAGGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTTTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGc < 1:45434/250‑1 (MQ=255) ccctcTGGTGGGCGCGGCG‑TGG‑TT‑CCATGTGCTGCCCTGGG‑AGGGAATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTGTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGccc < 1:489090/247‑1 (MQ=255) gCTGGTGGGCTCGGCT‑TGG‑AT‑CCTTGTTCTGCC‑TGGGTAGTGGGTTTTTTTTTTTTTTGCCGCATTGGCAGCTTCCTCCTTTTCG‑GGTCGGCAGCGGGCCAGGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACACGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGTCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGt < 2:1870/251‑1 (MQ=255) ggggcggcg‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATTTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCACCGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGAt < 1:195881/248‑1 (MQ=255) cgctttGGG‑GGGGGTTGTTTGTTTTGTTGGCCGCTGTGGCCGCGATC‑CCTTTTTCCGGTCTACAGCGGGCCAGGCCGGAAACCGCCTCGCGTAAAGGCGAGAAACTGTCACTGAAAGAACTCGGGCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGGTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTTGCCGATTATCATCATTACCGGCGAGCAg < 2:504079/246‑1 (MQ=255) ggg‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCa > 1:236458/1‑81 (MQ=255) ggg‑AGGGGATGTTTGTTTTGTTGTCCGCATTGGCAGCGACCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCa < 2:236458/81‑1 (MQ=255) | GGTGTGGTTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAA‑GATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCT‑ACTTGGTCCGCTGGTGGGCGCGGCG‑TGG‑AT‑CCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTC‑GGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAG > NC_000913/883939‑884425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |