| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 2,498,768 | 9486 bp→CCC | [pyrS]–fryB | [pyrS], pyrR, ypdC, fryA, ypdE, ypdF, fryC, fryB | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 2498768 | 2508253 | 9486 | 41 [0] | [0] 41 | [pyrS]–fryB | [pyrS],pyrR,ypdC,fryA,ypdE,ypdF,fryC,fryB |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 2498767 | 0 (0.000) | 40 (0.620) +CCC |
37/484 | 0.4 | 100% | coding (97/1698 nt) | pyrS | sensor histidine kinase PyrS |
| ? | NC_000913 | 2508254 = | 0 (0.000) | intergenic (‑12/+207) | fryB/glk | putative PTS enzyme IIB component FryB/glucokinase | |||||
GTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGA > NC_000913/2508011‑2508502 | gcattatcaagagatgcattgctaatacaccatttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCagg > 1:428527‑M2/244‑250 (MQ=255) atcaagagatgcattgctaatacaccatttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatcttcctgttctttctcatccgtatccgcctgtttcgcgcccCGGTCAGTGAt > 2:531305‑M2/239‑249 (MQ=255) gagatgcattgctaatacaccatttatctagcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagaggcccccgtgcacgaaatattcaacatgctgttggcggtcttcgatcgggcagcgttaatgattatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGAc < 2:80249‑M2/18‑1 (MQ=255) tgctaatgccccatttatctatcaatccccttttaaaattgtggtttttcctcagaaacggcctgctggtcaacagccttcaggttccctatgatagaggtttaattcttatgcagagtcgtcctttcccgaaatattcaacatgctgcgggcggtcttggagcgggcagcgttaatgcttatctgcctgttctttctcatccgttgccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGaaa < 2:326406‑M2/26‑1 (MQ=255) atttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggaagagttaatgcttttctgcctgttctttctcatccgtatccgcctgtttctcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGc > 1:367905‑M2/213‑250 (MQ=255) tatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcgggcttcgatcgggcagcgttaatgctttactgcctgttttttctcatccgtatccccctttttcgcgcccCGTTCAGTGATAGGTGACGAGTGAAATTATGAACGGGCaaa > 2:489284‑M2/210‑250 (MQ=255) taactatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcatctttcaggttccctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcaaccgtagccgcctggttcgcgcccCGGTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCaaa < 2:209267‑M2/41‑1 (MQ=255) atctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGtt > 1:355493‑M2/209‑223 (MQ=255) atctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgggcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGtt < 2:355493‑M2/15‑1 (MQ=255) tctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAAc > 1:45868‑M2/208‑250 (MQ=255) tttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagattcacccgtgcacgaaatattcaacatgctgctggcggtcttcgttcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc < 1:228887‑M2/56‑1 (MQ=255) aatttgggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaa < 2:513882‑M2/63‑1 (MQ=255) attgttgtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaa > 1:432813‑M2/188‑250 (MQ=255) cctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa < 1:427085‑M2/75‑1 (MQ=255) gctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATc < 1:49022‑M2/89‑1 (MQ=255) agaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatcggcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCtt < 2:419865‑M2/122‑1 (MQ=255) tattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTa < 1:327522‑M2/29‑1 (MQ=255) tattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcggtaatgcttatctgcctgttctttatcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGCCGAGTGAAATTa > 2:327522‑M2/116‑144 (MQ=255) attcagagtaccccgtccacgaaatattctacattctgctggcgttcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTTTAGAATTAATCCCGCTGTACGGAAGTGTGACGCATGTCGACCTTGTGAGgggacaaaa < 2:508496‑M2/136‑4 (MQ=255) tcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAg < 1:89541‑M2/138‑1 (MQ=255) agagccacccgtgcacgaaatattcaacatgctgctggcggtcttcgttcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGTAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGaaa < 1:37865‑M2/141‑1 (MQ=255) gagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGCTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGaaa > 1:140997‑M2/110‑250 (MQ=255) agtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttttctgcctgttatttctcatccgtatccgcctgtttcgcgaccCGTTCAGCGATAGTTTACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAAAGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGGCCTTGTGAGGGGACACAAAGaaaa > 2:150054‑M2/109‑250 (MQ=255) atattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGa < 1:509531‑M2/160‑1 (MQ=255) caacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTa < 1:232235‑M2/164‑1 (MQ=255) acatgcttcttgtggtcttcgatcgggcagcgttaatgcttatcggcctgttctttctcatccgtatccgcctggttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAGCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAAGAAACTGTATGATTTaaaa < 2:460101‑M2/167‑1 (MQ=255) tgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCACGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGCTTTaaa > 1:33020‑M2/82‑247 (MQ=255) gctgctgtcgttcttcgatcggtcagcgttaatgcttatctgcctgttctttctcatccgtatcctcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTAAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCATGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGAtttaaaatat < 1:302178‑M2/170‑4 (MQ=255) ggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCg < 1:9070‑M2/175‑1 (MQ=255) gtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTAACGGGagag < 1:379852‑M2/181‑1 (MQ=255) cgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTAcc > 1:97659‑M2/66‑251 (MQ=255) agcgttaatgcttatctccctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATGTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATGGAATAAACTGTATTATTTAAAAGATTATCGGGAGAGTTACCTCCCGatat < 1:395820‑M2/195‑1 (MQ=255) gttaatgcttatctgccttttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAGCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGAGTATCGGGAGAGGTACCTCCCGATATaaa < 2:182085‑M2/198‑1 (MQ=255) gttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCt < 2:235535‑M2/121‑1 (MQ=255) gttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCt > 1:235535‑M2/54‑174 (MQ=255) gttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATaa > 1:517265‑M2/54‑250 (MQ=255) catccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTa < 1:140420‑M2/224‑1 (MQ=255) ccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGt < 1:468457‑M2/228‑1 (MQ=255) ccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGAAAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGt > 1:485609‑M2/24‑251 (MQ=255) gtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGCGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAAtg > 1:394759‑M2/12‑251 (MQ=255) ccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAAGAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACTTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGa < 2:108818‑M2/249‑1 (MQ=255) | GTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGA > NC_000913/2508011‑2508502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |