Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,426	Δ1 bp	intergenic (‑16/‑216)	yhiN ← / → pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,426	0	G	.	100.0%	209.4 / NA	44	intergenic (‑16/‑216)	yhiN/pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/28);  total (16/28)

AACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGT  >  NC_000913/3637182‑3637654                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     acgctgcatgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAAc                                                                                                                                                                                                                               <  1:143031‑M2/22‑1 (MQ=255)       catgtgcacatcaaccacgacgactgcctggaaatcgccgtgttgaaaggatacatgggcgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggaacgggcaattgcagcgcttgccgacggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCatcttacaaaaacggacgt                                                                                                                                                                                                                          >  2:468318‑M2/224‑244 (MQ=255)                  caacctcgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgattacgttatcgcccagcgcggcgtgcggcatgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctgaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAg                                                                                                                                                                                                              <  1:241601‑M2/39‑1 (MQ=255)                  caaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTaaa                                                                                                                                                                                                               <  2:535648‑M2/38‑1 (MQ=255)                                gtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGt                                                                                                                                                                                                 <  1:479692‑M2/52‑1 (MQ=255)                                gtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTc                                                                                                                                                                                                <  1:34999‑M2/53‑1 (MQ=255)                                gtctggaaatcgccgtgtggaaaggtgacatggggggcgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatacgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTc                                                                                                                                                                                                <  2:409603‑M2/53‑1 (MQ=255)                                  ctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGc                                                                                                                                                                                              >  1:246116‑M2/197‑251 (MQ=255)                                    ggaaatcgccgtgttgaaaggtgacatggggtacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCAc                                                                                                                                                                                            <  2:156517‑M2/57‑1 (MQ=255)                                           gccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATc                                                                                                                                                                                     >  1:3109‑M2/188‑251 (MQ=255)                                              gtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAat                                                                                                                                                                                  <  1:137786‑M2/67‑1 (MQ=255)                                                          gacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAgg                                                                                                                                                                      <  1:146771‑M2/79‑1 (MQ=255)                                                           acatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGa                                                                                                                                                                     <  1:527939‑M2/80‑1 (MQ=255)                                                               gggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggcagattgagtctattattgggtcctttggagaacgatcaacaaaggtccactagcacacctgattcctctctaaatacactatccgaGTTGCGCTCCTCTTA‑AAAAACCGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCCCCAGCATCAATATTACTAAAAGGAAGt                                                                                                                                                                  >  2:121421‑M2/168‑250 (MQ=255)                                                               gggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaattccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGt                                                                                                                                                                  <  1:542914‑M2/83‑1 (MQ=255)                                                                ggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTAt                                                                                                                                                                >  1:270822‑M2/167‑251 (MQ=255)                                                                      gtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgcttcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATCTTACTAAAAGGAAGTATTTGCCt                                                                                                                                                          >  2:209249‑M2/161‑251 (MQ=255)                                                                         cagcattttgccgatgacgttatcgcccagcgcggcgtgcggctcgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGa                                                                                                                                                        >  1:334039‑M2/158‑250 (MQ=255)                                                                           gcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAAtt                                                                                                                                                     <  1:335416‑M2/96‑1 (MQ=255)                                                                                        gacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAatt                                                                                                                                        <  1:258819‑M2/109‑1 (MQ=255)                                                                                          cgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAattat                                                                                                                                      <  2:535392‑M2/111‑1 (MQ=255)                                                                                            ttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTAttt                                                                                                                                    >  1:489976‑M2/139‑251 (MQ=255)                                                                                             tatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTAttt                                                                                                                                    <  1:542339‑M2/113‑1 (MQ=255)                                                                                                               gcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATAc                                                                                                                  >  1:199813‑M2/120‑250 (MQ=255)                                                                                                                  gcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAggg                                                                                                              <  2:410116‑M2/135‑1 (MQ=255)                                                                                                                     cgggcatttgcagtgcttgccgaaggaggattgagtctcttattgggtactttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatcagaGTTGCGCTCATCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCCGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGc                                                                                                           >  1:463667‑M2/114‑251 (MQ=255)                                                                                                                              gcagtgcttgccgaaggaagattgaggctantattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCaaa                                                                                                   <  2:253699‑M2/146‑1 (MQ=255)                                                                                                                                 gtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCaaaaaaa                                                                                               <  1:11991‑M2/150‑1 (MQ=255)                                                                                                                                           aaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATAttt                                                                                     <  1:435550‑M2/160‑1 (MQ=255)                                                                                                                                              gaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctagccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCAc                                                                                  <  2:325705‑M2/163‑1 (MQ=255)                                                                                                                                                 gattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcactcctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACttt                                                                               <  1:294781‑M2/166‑1 (MQ=255)                                                                                                                                                  attgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTg                                                                              <  1:110854‑M2/167‑1 (MQ=255)                                                                                                                                                            ttattgggtcctttggtgaacgatcaacaaagggccactagcccacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAACAAGTCTATATTTCACTTTGCccgcgccgc                                                                    >  1:108691‑M2/75‑251 (MQ=255)                                                                                                                                                              attgggtcctttggtgaacgatcaacaaagggccactcgcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgccgcg                                                                   >  1:37758‑M2/73‑250 (MQ=255)                                                                                                                                                                tgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGaa                                                                 <  1:294029‑M2/180‑1 (MQ=255)                                                                                                                                                                             gaacgaacaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATg                                                   <  2:18695‑M2/194‑1 (MQ=255)                                                                                                                                                                                  atcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGcgccg                                              <  1:235003‑M2/199‑1 (MQ=255)                                                                                                                                                                                       caaagggccactagcacacctgattcctctctaaattccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAAGCCATACAGGGGGCAAATCAAAAAAAGTCTATATGTCCCTTTGCCCGCGCCGCGAAAGTCACTGATAAAGCGCCGCGTTc                                         <  2:324459‑M2/204‑1 (MQ=255)                                                                                                                                                                                       caaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTc                                         >  1:531359‑M2/48‑251 (MQ=255)                                                                                                                                                                                         aagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCAt                                       <  1:209249‑M2/206‑1 (MQ=255)                                                                                                                                                                                                      acacctgattcctctctcaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGCCGTGAATTGTAAAGCTTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGCAGTATTTGCCTGCATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATgg                          >  2:21055‑M2/33‑251 (MQ=255)                                                                                                                                                                                                         cctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGt                       >  1:88630‑M2/30‑251 (MQ=255)                                                                                                                                                                                                                       aaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGCCGTGAATCCTACAGATTCCTCAGGGGTCGCACCAGAATAAATATTACTCAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCTCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTACCGTCCTATGCt          >  2:197201‑M2/16‑250 (MQ=255)                                                                                                                                                                                                                             cctatccgcGTTGAGCTACTCTTA‑AAAAAACGGACGTGAATTGTCAAGATTCATCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAACGCAACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGAAACGTCCTATGCTACAtttg   >  2:86044‑M2/10‑251 (MQ=255)                                                                                                                                                                                                                                atccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTTGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACAtttgt  <  1:344097‑M2/243‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     AACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGT  >  NC_000913/3637182‑3637654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑