Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,586	G→A	Q249Q (CAG→CAA)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,586	0	G	A	100.0%	260.1 / NA	74	Q249Q (CAG→CAA)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/41);  total (33/41)

GTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCA  >  NC_000913/2251341‑2251829                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gTGGAAGCTAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATC‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacg                                                                                                                                                                                                                                                 >  2:650181/1‑250 (MQ=255)   ggAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGAcc                                                                                                                                                                                                                                              <  1:605277/251‑1 (MQ=255)    gAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCg                                                                                                                                                                                                                                             >  1:546712/1‑251 (MQ=255)             ccccgggtgggCGTCGGCATTGATCCCTGCCATGCTTGCGCTGCAGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAACCAGTCGCGGATTTTGCCAGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCGGGATCATGCAAGACGACCGTTTCGACgg                                                                                                                                                                                                                                    <  2:155010/242‑1 (MQ=255)                  cgTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTc                                                                                                                                                                                                                               >  1:418187/1‑251 (MQ=255)                             gCATTGATCCCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc                                                                                                                                                                                                                    <  1:358476/251‑1 (MQ=255)                                 tGATCCCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa                                                                                                                                                                                                                 <  1:436765/250‑1 (MQ=255)                                  gATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑AGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGCTCCTCGAAAc                                                                                                                                                                                                               >  2:267554/1‑251 (MQ=255)                                   aTACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCCAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAcc                                                                                                                                                                                                              >  2:202391/1‑251 (MQ=255)                                      ccTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTTAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc                                                                                                                                                                                                           <  1:259553/251‑1 (MQ=255)                                            aTGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg                                                                                                                                                                                                     >  1:383692/1‑251 (MQ=255)                                                 ttCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat                                                                                                                                                                                                >  1:421809/1‑251 (MQ=255)                                                 ttCGCTGCCGGGTATGATTTGCGTACTCAAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCCCCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGCCCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGata                                                                                                                                                                                                 <  2:41035/250‑1 (MQ=255)                                                     cTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTgg                                                                                                                                                                                            >  1:172764/1‑251 (MQ=255)                                                       gCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCTTACTGTCGGCTTTAAGTATCTGCGCGGGCTGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGAGAAGGCAATATCGTTCGTGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGAATTACGCTGATCCTCGAAACCATCACCCCGGATATCTggg                                                                                                                                                                                           >  2:421400/1‑250 (MQ=255)                                                       gCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGc                                                                                                                                                                                          <  1:476099/251‑1 (MQ=255)                                                       gCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGc                                                                                                                                                                                          >  2:676033/1‑251 (MQ=255)                                                       gCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGc                                                                                                                                                                                          >  1:407980/1‑251 (MQ=255)                                                       gCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGc                                                                                                                                                                                          >  1:630438/1‑251 (MQ=255)                                                         cGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCag                                                                                                                                                                                        <  1:38477/251‑1 (MQ=255)                                                                    tGCGTACTCCAGCCGAATGCTAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGGATCTGCGCGGGATGCCCGTTAACGATG‑CGAAAAGCTCCTTTTGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGTTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACTGCATTTCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGc                                                                                                                                                                              <  1:405020/250‑1 (MQ=255)                                                                       gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAAGATCTGCGCGGGATGCACCTTACCGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGCTCATGATGAGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTACGCTGCTCCTCCAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTgg                                                                                                                                                                          >  2:523646/1‑251 (MQ=255)                                                                         aCTCCAGCCGAATGCGAGAAAACATTCGCGGNTTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt                                                                                                                                                                        <  2:348788/251‑1 (MQ=255)                                                                                ccGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAgcg                                                                                                                                                                 >  1:719864/1‑251 (MQ=255)                                                                                  gAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAgcgc                                                                                                                                                                >  1:369117/1‑250 (MQ=255)                                                                                   aaTGCGAGAAAACATTCGCGTATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGCGATCGCCTGGCTGAAAGCGca                                                                                                                                                               >  2:30709/1‑250 (MQ=255)                                                                                      gCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaaca                                                                                                                                                            <  1:500902/250‑1 (MQ=255)                                                                                       cGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACaaa                                                                                                                                                          <  1:192950/251‑1 (MQ=255)                                                                                           aaaaCATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGa                                                                                                                                                      <  2:418187/251‑1 (MQ=255)                                                                                                aTTCGCGGATTTTGCCCGTACTGTAGGCTTTTAGTATCTGCGCGGGAGGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGACGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCAGGATGCGTTCCGCTGGATCATGCAAGACGACCTATTCGACGGCATTCCGCTGATCCTCGAAACCATAAAACCGAATATCTGGGCAGAAGAGATCGACTGGCTGAAAGCGCAACAACCTaaaaaa                                                                                                                                                  >  2:726202/1‑250 (MQ=255)                                                                                                 ttCGCGGATTTTGCCCGTACTGCCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTGTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAg                                                                                                                                                 <  2:442659/250‑1 (MQ=255)                                                                                                    gcgGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGt                                                                                                                                             <  1:161541/251‑1 (MQ=255)                                                                                                        aTTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGcc                                                                                                                                         >  1:439220/1‑251 (MQ=255)                                                                                                                cGTACTGTCGTCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgc                                                                                                                                 <  1:439824‑M1/251‑3 (MQ=255)                                                                                                                cGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGTTC‑CGAAAAGCACCTTGGGTAGCCGCGTTGGCCGCCATCATAGCCTCGGTTAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTGCGACGGCAGTCCGCTGATCCGCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAATCTGAAAAAGCGGTAGCCTGAAGAgc                                                                                                                                 <  1:421400‑M1/251‑3 (MQ=255)                                                                                                                   aCTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcac                                                                                                                               <  1:30709‑M1/250‑5 (MQ=255)                                                                                                                   aCTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcac                                                                                                                               <  2:423448‑M1/250‑5 (MQ=255)                                                                                                                        cggatttaattATCTGCGCGGGATGCACGTTNACGATG‑CGAAAAGGTCCTTTGGCAGCCGCGTTGACCGCCAGCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGAGATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGTCTGAAGAgcacgtcctc                                                                                                                         <  2:319862‑M1/247‑11 (MQ=255)                                                                                                                         ggcgttcagtagcggcgcgggatgccccataaCGATG‑CGAAAAGCACCTCTGGCAGCCGCGTTGACCGCCATCATAGCCTAGGTAAAGGCAATATCGGTCCTGAGGCGTTCCGCTGGATCATGCAAGACGACCGGTTCGACGGCATGCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAATAGCTCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGGCTGCAGAgcatgtcctcg                                                                                                                        <  2:609118‑M1/237‑12 (MQ=255)                                                                                                                             ttaagtgttggggggggatgccccttaacg‑tGACGAAAAGCACTTTTGGCAGGCGCGTTGACCGCCATCATTGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAGCAAACTGAAAAAGCGGTAGCCTGAAGAccacgtcctctggt                                                                                                                     <  2:668645‑M1/236‑15 (MQ=255)                                                                                                                             ttAAGTGTCGGCGCTGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCTCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACACGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAATAAACTGAAAAAGCTGTAGCCTGAAGAgcacgtcctctggt                                                                                                                     <  2:640316‑M1/250‑15 (MQ=255)                                                                                                                                         gcgGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGACATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACAGTTTCGCCGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattacca                                                                                                         >  1:411729‑M1/1‑224 (MQ=255)                                                                                                                                           gggATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGTCCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCt                                                                                                                                                                            <  2:425845/181‑1 (MQ=255)                                                                                                                                           gggATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCt                                                                                                                                                                            >  1:425845/1‑181 (MQ=255)                                                                                                                                            ggATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccc                                                                                                     <  1:467889‑M1/251‑31 (MQ=255)                                                                                                                                            ggATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccc                                                                                                     <  1:323569‑M1/251‑31 (MQ=255)                                                                                                                                            ggATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccc                                                                                                     <  1:676033‑M1/251‑31 (MQ=255)                                                                                                                                             gATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCGGCCTCGTTGACCGCCATCATAGACTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGATGGATCATGCAAGACGACCGTTTCGCCGGCATTCCGCTGATCCTCGAAACCACCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCACCAAACTGAAAAAGCGGAAGCCTGAAGAgaacgccctctggtacaaaataaccaacact                                                                                                    >  2:563053‑M1/1‑220 (MQ=255)                                                                                                                                                gCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGCCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCAGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCATGAAGAgcacgtcctacggtaaaaaattacaaacactgtc                                                                                                 >  2:380542‑M1/1‑217 (MQ=255)                                                                                                                                                 cACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGaaa                                                                                                                                                                    <  2:221517/183‑1 (MQ=255)                                                                                                                                                 cACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGaaa                                                                                                                                                                    >  1:221517/1‑183 (MQ=255)                                                                                                                                                   ccTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGGgcacgtcctctggtaaaaaattaccaaccctgtcaat                                                                                              >  1:238713‑M1/1‑214 (MQ=255)                                                                                                                                                   ccTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaat                                                                                              >  1:410877‑M1/1‑214 (MQ=255)                                                                                                                                                       aaCGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCCGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccac                                                                                          >  1:99634‑M1/1‑210 (MQ=255)                                                                                                                                                          gATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaag                                                                                       <  1:335936‑M1/251‑45 (MQ=255)                                                                                                                                                                  aaaGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatat                                                                                  >  1:260901‑M1/1‑200 (MQ=255)                                                                                                                                                                    aGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagag                                                                              <  1:315478‑M1/251‑54 (MQ=255)                                                                                                                                                                       ccttgtgctagccgttttgaccgccatcatagccTCGGGGAAGGCAATATCGGTCATGATGCGGTCCGCGGGATCATGCAAGACGCCCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCTGATATCTGGGCAGAAGAGATCGCCTGTCTGAAAGCGCAATAAACTGAAAAAGCGGTAGCCTGAAGAtcacgtcctctgttaaaaaattaccatccctgtcaatccacaagaatacagatact                                                                           <  2:653776‑M1/235‑57 (MQ=255)                                                                                                                                                                           ttGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgc                                                                         <  1:632670‑M1/249‑59 (MQ=255)                                                                                                                                                                             ggCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagcaactgcaa                                                                       >  2:696543‑M1/1‑189 (MQ=255)                                                                                                                                                                                   cgcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGCCGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccaccagactatagatactgcaaagacggtt                                                               >  1:356721‑M1/1‑183 (MQ=255)                                                                                                                                                                                      gTTGACCGCCATCAGAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGGTCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaa                                                            <  2:217406‑M1/251‑72 (MQ=255)                                                                                                                                                                                         gACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaaca                                                         <  2:383692‑M1/251‑75 (MQ=255)                                                                                                                                                                                               catcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagattctgcaaagacggtttaaacatgtgtt                                                   <  1:590906‑M1/251‑81 (MQ=255)                                                                                                                                                                                                      gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatattt                                            <  1:193996‑M1/251‑88 (MQ=255)                                                                                                                                                                                                       ccgaggtgaaGGCAATAGCGGGCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaagtaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatattta                                           <  2:624444‑M1/247‑89 (MQ=255)                                                                                                                                                                                                              gacgggaatatcggTCATGATGCGTGCCGCTGGATCATGCAAGACGACCGTTTCGACGGAATTCCGCAGATCCTCGAAACCATCAACACGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAATCGCAACAAACTGAAAAAGAGGTAGACGGAAGAgcacgtcctctggtaaaaacttaccaaccctgtcactccacacgaatatagatactgcaaagacggtttaaacatgtgttcatagttaaacatgt                                    >  2:207284‑M1/7‑156 (MQ=255)                                                                                                                                                                                                               aaGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtg                                   <  1:36417‑M1/251‑97 (MQ=255)                                                                                                                                                                                                                       atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagt                           >  1:273188‑M1/1‑147 (MQ=255)                                                                                                                                                                                                                                gatgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgt                   <  1:279101‑M1/250‑113 (MQ=255)                                                                                                                                                                                                                                     gTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacg             >  1:672843‑M1/1‑133 (MQ=255)                                                                                                                                                                                                                                             ggATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgtaaagtaa     <  2:172764‑M1/251‑127 (MQ=255)                                                                                                                                                                                                                                                 cATGCAAGGCGACCGTTTCTACGGCATTCCGCTGAGTCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAAATGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacggaaagtaaatt  <  2:202803‑M1/250‑130 (MQ=255)                                                                                                                                                                                                                                                 cATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaatttaccacccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacgtaaagtaaatt  >  2:60190‑M1/1‑121 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATG‑CGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCA  >  NC_000913/2251341‑2251829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑