Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,332	T→C	intergenic (+69/‑20)	ydfG → / → rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,332	0	T	C	100.0%	257.0 / NA	68	intergenic (+69/‑20)	ydfG/rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/37);  total (31/37)

GGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAATTCGTCCGCAACGTGGCAGCT  >  NC_000913/1628090‑1628571                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                ggCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGAATGAATGTCAACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCAATCACt                                                                                                                                                                                                                                         >  1:647633‑M1/1‑250 (MQ=255)     aTGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacat                                                                                                                                                                                                                                     <  2:462468‑M1/251‑6 (MQ=255)                    aaaaaaCCTATCAAAATACAGTTGCATTGAAGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggac                                                                                                                                                                                                                      >  1:549885‑M1/1‑231 (MQ=255)                          ccTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttag                                                                                                                                                                                                                <  1:185781‑M1/251‑27 (MQ=255)                               cAAAATACCGTTGCATTGACGCCAGAAGGTGTCAGCGAAGCCGTCTGGGGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACAGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTGGACTCATGCAGTTAACCAACTCACtacatgaccagcggcaggacctttcggtacg                                                                                                                                                                                                           >  1:97726‑M1/1‑220 (MQ=255)                               cAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccg                                                                                                                                                                                                           <  1:480763‑M1/251‑32 (MQ=255)                                   aTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttct                                                                                                                                                                                                       >  1:538284‑M1/1‑216 (MQ=255)                                    tACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctc                                                                                                                                                                                                      >  1:162231‑M1/1‑215 (MQ=255)                                     aCCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCCACGCTGCCTGCTCACGGCAATATCAATACCCTGGAAATGATGCAGGTTACCAAAAGCTACGCCGGACTGAATGTCCAAAGTCAGTAATTTTTATAACCGGCGTAACTGCCGGGTTATTGCTTGTCACAACAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctc                                                                                                                                                                                                      >  2:548983‑M1/1‑214 (MQ=255)                                     aCCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctca                                                                                                                                                                                                     <  1:147691‑M1/251‑38 (MQ=255)                                       cGTTGCATTGACGCCCGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggcccgttctcaat                                                                                                                                                                                                   >  1:511101‑M1/1‑212 (MQ=255)                                          tGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaatac                                                                                                                                                                                                 <  1:142304‑M1/250‑42 (MQ=255)                                               tGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaatt                                                                                                                                                                                           >  2:309095‑M1/1‑204 (MQ=255)                                                gACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattc                                                                                                                                                                                          <  1:492586‑M1/251‑49 (MQ=255)                                                    ccAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaact                                                                                                                                                                                      <  1:290488‑M1/251‑53 (MQ=255)                                                    ccAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATAGCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATTCCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacaggaacagcggcaggaccgttagggccgttctcaataccaaattaaact                                                                                                                                                                                      <  2:628001‑M1/251‑53 (MQ=255)                                                     cAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaactt                                                                                                                                                                                     <  1:309095‑M1/251‑54 (MQ=255)                                                             gTCAGCGAAGACGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATAGCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgatt                                                                                                                                                                              <  2:139948‑M1/250‑61 (MQ=255)                                                                   gAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtcagttctcaataccaaattcaacttcctgattctcagtt                                                                                                                                                                       >  2:83395‑M1/1‑184 (MQ=255)                                                                    aaGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagtta                                                                                                                                                                      <  1:300555‑M1/251‑69 (MQ=255)                                                                    aaGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaactgcctgattctcagtta                                                                                                                                                                      <  1:166931‑M1/251‑69 (MQ=255)                                                                         gTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgtt                                                                                                                                                                 >  1:190992‑M1/1‑178 (MQ=255)                                                                                  gtgtCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggccctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcg                                                                                                                                                        >  2:301270‑M1/1‑169 (MQ=255)                                                                                  gtgtCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcg                                                                                                                                                        >  1:301751‑M1/1‑169 (MQ=255)                                                                                       aaCGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgct                                                                                                                                                   >  1:88007‑M1/1‑164 (MQ=255)                                                                                             gcctgcTCACGTCAATAGCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTGTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaa                                                                                                                                              <  2:179573‑M1/250‑93 (MQ=255)                                                                                              cctgcTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaatt                                                                                                                                            <  1:22348‑M1/251‑95 (MQ=255)                                                                                                      cGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgatgaga                                                                                                                                     >  2:624015‑M1/1‑149 (MQ=255)                                                                                                          catatcaatacCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACCGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttactggtttgaaatcgttgctctgaattgctgggcact                                                                                                                                  >  2:191024‑M1/2‑145 (MQ=255)                                                                                                               caataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaancgttgctctgaattgctgagaaatagacaa                                                                                                                            >  2:330349‑M1/1‑140 (MQ=255)                                                                                                                        ggAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacctcttt                                                                                                                  >  2:541471‑M1/1‑131 (MQ=255)                                                                                                                           aaggatgccgGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcaggtaatgttttgaaatcgttgctctgaattgctgagaaatagacaaactcatctttgct                                                                                                               <  2:121684‑M1/248‑124 (MQ=255)                                                                                                                             tgatgCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgc                                                                                                             >  2:444243‑M1/1‑126 (MQ=255)                                                                                                                                  ccGGTTACCCAAAGCTAGGCCGGACTGAATGNCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGATATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacattaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctatgctgccatct                                                                                                        <  2:353976‑M1/251‑131 (MQ=255)                                                                                                                                    ggTTACCCAAAGATATGACGGACTGAATGACCACCGTCAGTAATTTTTATACCCGGCGTAACTACCGGGTTATAGCTTGTCACAAAAAAGTGGAAGACTCATGTAGTTAACCCACTCACtacatgaacagcggaaggccctttaggtcagttcccaataccaaattcaacttcctgattctccgtttatgttttgaaatcgttgctctgaattgctgagaattagacaaaaacatctttgctgccatcttt                                                                                                      >  2:687541‑M1/1‑119 (MQ=255)                                                                                                                                     ggtacCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattccccattaatgttttgaaaacgttgctctgaattgctgagaaatagacaaacacaactttgctgccatctttc                                                                                                     >  2:136393‑M1/3‑118 (MQ=255)                                                                                                                                     gTTACCCAAAGCTATGGCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttc                                                                                                     <  2:114613‑M1/251‑134 (MQ=255)                                                                                                                                       tACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcg                                                                                                    <  2:538284‑M1/250‑135 (MQ=255)                                                                                                                                           cAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtg                                                                                               <  1:145485‑M1/251‑140 (MQ=255)                                                                                                                                              aGCTAGGCCGGAATGAATGTCCACCTTCAGTAAATTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacattaacagcggcaggacctttagggccgttttcaataccaaattcagcttcctgattctcagttaatgttttgaaatcgttgctctgaattgcttagaaatatataaatacatctttgctgccagctttcggcatgatg                                                                                            <  2:673109‑M1/251‑143 (MQ=255)                                                                                                                                                cTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcagggcctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatga                                                                                           >  2:249255‑M1/1‑107 (MQ=255)                                                                                                                                                    gCCGGGCTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaacc                                                                                       <  2:527865‑M1/250‑148 (MQ=255)                                                                                                                                                       ggACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaa                                                                                   <  1:205259‑M1/251‑152 (MQ=255)                                                                                                                                                         aCTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacc                                                                                 >  2:707512‑M1/1‑98 (MQ=255)                                                                                                                                                            gAATGTCCGCCACCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgcaatctttcggcgtgatgaaaccaaaaccttt                                                                              <  2:620971‑M1/251‑157 (MQ=255)                                                                                                                                                            gAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaaccttt                                                                              <  2:190992‑M1/251‑157 (MQ=255)                                                                                                                                                             aaTGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttt                                                                             <  1:202861‑M1/251‑158 (MQ=255)                                                                                                                                                                       gTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgttgaaaccaaaacctttttctgggtta                                                                    >  2:496934‑M1/1‑84 (MQ=255)                                                                                                                                                                         cAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaa                                                                  <  1:485286‑M1/250‑169 (MQ=255)                                                                                                                                                                          aGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGGGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttttaaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgataaatagacaaactcatctttgctgccatctttcggcgtgattaaaccaaaacctttttcagggttaaacc                                                                <  2:590228‑M1/251‑171 (MQ=255)                                                                                                                                                                           gTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgtttctctgaattgctgagaaatagacaaccacatctttgctgccatctttcggcgtgatgaaaccaaaccctttttcagggttaaaccc                                                               >  2:661034‑M1/1‑80 (MQ=255)                                                                                                                                                                             aaTTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatt                                                             >  1:350983‑M1/1‑78 (MQ=255)                                                                                                                                                                                  ttATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcac                                                         <  2:128487‑M1/250‑178 (MQ=255)                                                                                                                                                                                  ttATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcac                                                         <  1:492313‑M1/250‑178 (MQ=255)                                                                                                                                                                                        ccGGCGTAACTGCCGGGTTATTGCTGGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaagaccaaattcaacgtcctgatgctcagttaatgttttgaaatcgttgctctgtattgctgagaaatagacaaacacatcgttgctgccagctttcggcgtgatgaaaccaaaacctttttcagggttaagccacgtcactaaacca                                                  <  2:236762‑M1/251‑185 (MQ=255)                                                                                                                                                                                          ggCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgtgaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaacaaaacctttttcagggttaaaccatttcactaaaccagt                                                >  2:686669‑M1/1‑65 (MQ=255)                                                                                                                                                                                          ggCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccagctttcggcgtgat                                                                                             <  2:84410‑M1/206‑142 (MQ=255)                                                                                                                                                                                          ggCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgctgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgat                                                                                             >  1:84410‑M1/1‑65 (MQ=255)                                                                                                                                                                                            cGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtca                                              <  1:262846‑M1/251‑189 (MQ=255)                                                                                                                                                                                                      gggTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaacagtcattttgtt                                       >  1:521631‑M1/1‑53 (MQ=255)                                                                                                                                                                                                                  gTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaactagtcattttgttagacataattattac                        <  1:640878‑M1/251‑211 (MQ=255)                                                                                                                                                                                                                          aaaGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaactacctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgcttccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttgaa                <  2:413249‑M1/251‑219 (MQ=255)                                                                                                                                                                                                                                 tAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttgaagaaatt          >  1:256429‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                  aGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccgtttgaagaaattag        <  2:88007‑M1/251‑227 (MQ=255)                                                                                                                                                                                                                                   gACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttgaagaaattagc       <  1:218998‑M1/251‑228 (MQ=255)                                                                                                                                                                                                                                        ctgCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgatctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccaggcattttgttagacataattcttaccttttgcagaaattagcccttg  >  2:232642‑M1/2‑19 (MQ=255)                                                                                                                                                                                                                                             gTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatct                                                                                                        >  2:273984‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                                             gTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatct                                                                                                        <  1:273984‑M1/144‑131 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                GGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAATTCGTCCGCAACGTGGCAGCT  >  NC_000913/1628090‑1628571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑