Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,992	C→A	intergenic (‑209/‑26)	leuS ← / → ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,992	0	C	A	100.0%	296.0 / NA	80	intergenic (‑209/‑26)	leuS/ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/39);  total (41/39)

TTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCGAACGTGATATCGACGCACTGGTGGAACAGGCGCGCGAGCGCGTAATAAAAACAGGGGAGTTAACGCGAACCGAGGTCGATGAGCTGACGCGAGCTGTCAGACGTGACCTGGAAGAGTTCGCCATGAGCTATGAAGAGAGCCTGAAAGAAGAA  >  NC_000913/674746‑675236                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ttGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGt                                                                                                                                                                                                                                                   <  2:456007/250‑1 (MQ=255)    gATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAAc                                                                                                                                                                                                                                               <  1:121105/251‑1 (MQ=255)     aTTCTATCTCTTCCGTGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTGTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTGTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACGCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACg                                                                                                                                                                                                                                              <  2:280249/251‑1 (MQ=255)     aTTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACg                                                                                                                                                                                                                                              >  1:596995/1‑251 (MQ=255)        cTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAGCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGAcc                                                                                                                                                                                                                                           >  2:579204/1‑251 (MQ=255)          aTCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTACGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCg                                                                                                                                                                                                                                         >  2:392382/1‑251 (MQ=255)              ctTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCgg                                                                                                                                                                                                                                        <  1:425473/248‑1 (MQ=255)               ttCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCggg                                                                                                                                                                                                                                       >  1:271720/1‑248 (MQ=255)                tCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGgag                                                                                                                                                                                                                                     <  1:117089/249‑1 (MQ=255)                  cGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCTGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATg                                                                                                                                                                                                                                  >  1:509305/1‑250 (MQ=255)                        gTATTGCTCTTGCATGGCAGCCAGTGGTCCTNGTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTTGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgc                                                                                                                                                                                                                           <  2:362922‑M1/251‑3 (MQ=255)                            tGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatc                                                                                                                                                                                                                        <  1:207613‑M1/250‑6 (MQ=255)                             gCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccg                                                                                                                                                                                                                      >  1:45254‑M1/1‑244 (MQ=255)                               ccttGCATGGCAGCCAGTGGGCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggt                                                                                                                                                                                                                    <  2:38671‑M1/250‑10 (MQ=255)                                ctTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtg                                                                                                                                                                                                                   >  1:160520‑M1/1‑241 (MQ=255)                                   gCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagc                                                                                                                                                                                                                <  1:709226‑M1/251‑14 (MQ=255)                                   gCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagc                                                                                                                                                                                                                >  1:682194‑M1/1‑238 (MQ=255)                                   gCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagc                                                                                                                                                                                                                >  1:486698‑M1/1‑238 (MQ=255)                                     aTGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgt                                                                                                                                                                                                              <  1:189609‑M1/251‑16 (MQ=255)                                         cAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccg                                                                                                                                                                                                          <  2:299263‑M1/251‑20 (MQ=255)                                            ccAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaa                                                                                                                                                                                                       <  2:651837‑M1/251‑23 (MQ=255)                                             cAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccgatgagcgtgccgaaat                                                                                                                                                                                                      >  1:352464‑M1/1‑228 (MQ=255)                                                       gTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACACAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgt                                                                                                                                                                                             >  1:87305‑M1/1‑218 (MQ=255)                                                                   gCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgat                                                                                                                                                                                <  1:515604‑M1/251‑46 (MQ=255)                                                                   gCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAAGTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgat                                                                                                                                                                                <  2:590009‑M1/251‑46 (MQ=255)                                                                     acgtttgcccgctttggggtgttttttcagatccgcatagCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACATAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgata                                                                                                                                                                               <  2:118908‑M1/233‑47 (MQ=255)                                                                       aaaatGTAGCGTTGAGGTTGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataaga                                                                                                                                                                            <  2:601950‑M1/250‑50 (MQ=255)                                                                         aaTGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgnaatatgggatgtattccggcacgataaga                                                                                                                                                                            >  2:326369‑M1/1‑200 (MQ=255)                                                                         aaTGTAGCGTTGAGGTGGTGTTTCAGATCCGNATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccagtgagcgtgccgaaatatgggatgtattccggcacgataagaag                                                                                                                                                                          <  2:352464‑M1/251‑52 (MQ=255)                                                                             tAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggat                                                                                                                                                                      >  1:251750‑M1/1‑196 (MQ=255)                                                                             tAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCATCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggat                                                                                                                                                                      >  2:139115‑M1/1‑196 (MQ=255)                                                                                    gAGGTGGTTTTTCATATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttac                                                                                                                                                               <  2:160520‑M1/251‑63 (MQ=255)                                                                                                 aGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgc                                                                                                                                                    >  1:367035‑M1/1‑176 (MQ=255)                                                                                                  gATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggc                                                                                                                                                                                     >  2:340994‑M1/1‑175 (MQ=255)                                                                                                  gATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggc                                                                                                                                                                                     <  1:340994‑M1/215‑41 (MQ=255)                                                                                                     ccacagagcatagaccaaacGTCAGCGTAAAACCAGACTTTCGCGCACTCGACGGTGAAGTGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGTAACAAAGAACGTCTATTATTATAGTAAGTTAACTACCCGGGATATGAAACGgcaaccggtgagcttgacgaaatatgggatgtattcctgcacgataagaagggattatttacgtcgctgacgcagact                                                                                                                                               <  2:488584‑M1/244‑79 (MQ=255)                                                                                                     ccGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactc                                                                                                                                              <  2:176267‑M1/251‑80 (MQ=255)                                                                                                       gcatagcataGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgatacgaagggattatttacgtcgctgacgcagactcat                                                                                                                                            >  2:158714‑M1/1‑170 (MQ=255)                                                                                                           agcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaa                                                                                                                                         <  1:244968‑M1/250‑85 (MQ=255)                                                                                                               tagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatacggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgaagactcatcaacac                                                                                                                                      >  1:521270‑M1/1‑162 (MQ=255)                                                                                                                 gcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagc                                                                                                                                   >  2:542710‑M1/1‑160 (MQ=255)                                                                                                                       actaccgcgtCAAAACAGCCGTTCGCGCACTNGACGTTGCAATGATGCCCGGATTACTCATACATGAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTAGAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagtgattatttacgtcgctgacgcagactcatcaacacagcagcaaaa                                                                                                                            <  2:336397‑M1/247‑98 (MQ=255)                                                                                                                           ccGCGTCAAACCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaa                                                                                                                        <  2:377104‑M1/251‑102 (MQ=255)                                                                                                                            cgcgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaa                                                                                                                        >  2:150664‑M1/1‑149 (MQ=255)                                                                                                                             gcgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaac                                                                                                                      <  1:358397‑M1/251‑104 (MQ=255)                                                                                                                               gTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCCTACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcngactcatcaacacagcagcaaaaccaaaca                                                                                                                     >  2:339061‑M1/1‑146 (MQ=255)                                                                                                                                 cAAAACCGCCTGTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgtgcgtgccgaaataggggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaat                                                                                                                   <  2:645977‑M1/250‑107 (MQ=255)                                                                                                                                   aaaCAGCCTTTAGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgaagcagactcatcaacacagcagcaaaaccaaacaatgcc                                                                                                                >  1:703309‑M1/1‑142 (MQ=255)                                                                                                                                    aaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccg                                                                                                               <  1:488439‑M1/251‑111 (MQ=255)                                                                                                                                         ccTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagcagggattatttacgtcgctgacgcagactcatcaacacagcagcaaacccaaacaatgccgtcagc                                                                                                          >  1:47741‑M1/1‑136 (MQ=255)                                                                                                                                          cTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagc                                                                                                          >  1:451124‑M1/1‑135 (MQ=255)                                                                                                                                              cgcgCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacggcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcaccc                                                                                                      <  1:656259‑M1/250‑120 (MQ=255)                                                                                                                                               gcgcACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccac                                                                                                    <  1:348816‑M1/251‑122 (MQ=255)                                                                                                                                                        aCGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacatcggccag                                                                                           <  1:553000‑M1/251‑131 (MQ=255)                                                                                                                                                             gAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgcc                                                                                      >  2:141744‑M1/1‑116 (MQ=255)                                                                                                                                                               aaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggatta                                                                                                                                                                    >  2:158324‑M1/1‑114 (MQ=255)                                                                                                                                                               aaTGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggatta                                                                                                                                                                    <  1:158324‑M1/171‑58 (MQ=255)                                                                                                                                                                   atgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacg                                                                                >  1:199339‑M1/1‑110 (MQ=255)                                                                                                                                                                           attactCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatg                                                                        >  2:312057‑M1/1‑102 (MQ=255)                                                                                                                                                                                  aTACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggggtg                                                                   >  1:595623‑M1/1‑95 (MQ=255)                                                                                                                                                                                     cATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtagggtgtgggtcc                                                              >  2:85800‑M1/1‑92 (MQ=255)                                                                                                                                                                                      aTTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtctctgacgcagactcatcaacacagcagcaaaaccaaacagtgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccg                                                             <  1:354980‑M1/251‑161 (MQ=255)                                                                                                                                                                                      aTTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccg                                                             >  2:113291‑M1/1‑91 (MQ=255)                                                                                                                                                                                          aTTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtgg                                                         <  2:251750‑M1/251‑165 (MQ=255)                                                                                                                                                                                             tACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtc                                                       <  1:701176‑M1/250‑167 (MQ=255)                                                                                                                                                                                                       gtgGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccgggggtcggctgcacgtt                                            >  2:285915‑M1/1‑74 (MQ=255)                                                                                                                                                                                                         gggCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcccccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgtta                                           <  2:517606‑M1/250‑179 (MQ=255)                                                                                                                                                                                                         gggCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgtt                                            >  1:424116‑M1/1‑72 (MQ=255)                                                                                                                                                                                                            cGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtgg                                       >  1:330719‑M1/1‑69 (MQ=255)                                                                                                                                                                                                                 tAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaa                                   <  2:87305‑M1/250‑187 (MQ=255)                                                                                                                                                                                                                          aaGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccgggggtcggcgtcacgttaggggttaacacctcgcgg                         >  2:16726‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                           aGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcggg                        >  1:368544‑M1/1‑54 (MQ=255)                                                                                                                                                                                                                               aCAAAGAACGTCTATTATTATAGTAAGTTAACGTTCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatggaatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacgcagcagtaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggctaacgcctcgcgggtgaa                    <  2:596995‑M1/251‑202 (MQ=255)                                                                                                                                                                                                                                cAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaac                   <  1:392382‑M1/251‑203 (MQ=255)                                                                                                                                                                                                                                   aGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacacacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgc                >  1:518898‑M1/1‑46 (MQ=255)                                                                                                                                                                                                                                    gAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtccgcacccacagcggccaggtgccggtacgtgcgtatggtgtgagtacggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcg               >  1:496212‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                                     aaCGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgg              <  1:139115‑M1/251‑208 (MQ=255)                                                                                                                                                                                                                                        gTCTATTATTATAGTAAGTTAAAGACCCGGGAGATGAAACGgcatccggtgagcattccgaaatatgggaggtatttcggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcaccaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggat           >  1:477911‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                                            attattatAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggggtgagtccgggggtcggcggcatcatagagtttaaaacatcgcgggtaaactgcgggatcatc       >  2:111419‑M1/1‑37 (MQ=255)                                                                                                                                                                                                                                                  ataGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggatcatcgcctg  >  1:429127‑M1/1‑31 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCGAACGTGATATCGACGCACTGGTGGAACAGGCGCGCGAGCGCGTAATAAAAACAGGGGAGTTAACGCGAACCGAGGTCGATGAGCTGACGCGAGCTGTCAGACGTGACCTGGAAGAGTTCGCCATGAGCTATGAAGAGAGCCTGAAAGAAGAA  >  NC_000913/674746‑675236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑