Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	97.5%	265.8 / NA	80	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (53/25);  minor base C (0/2);  total (53/27)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674272‑674762                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       aCTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGcc                                                                                                                                                                                                                                                    >  1:337256/1‑251 (MQ=255)     gttggttccccattccGGTCTGGTCGTCCGGGCCCCATTTGACCGAAGAAGTTTTTTTATATACAAGGCCTTTTTTAACCAGCCCGGTGAAGAATTCCTGTTCCCACCGGTAGTATTCGGGCTTACAGGTGGCCTGCGCGCGGTTCAAGTCATAACCAAT‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCGCGGTGCCGGGGCGTTGTTTTTTTTCCCCGCCACGCCTTc                                                                                                                                                                                                                                                 <  2:222297/235‑1 (MQ=255)                      gTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGGGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCCGGCAGACCAAAcgc                                                                                                                                                                                                                               >  2:637674/1‑251 (MQ=255)                        cTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTc                                                                                                                                                                                                                            >  1:672596/1‑251 (MQ=255)                              gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc                                                                                                                                                                                                                       >  1:524015/1‑251 (MQ=255)                              gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc                                                                                                                                                                                                                       >  1:252292/1‑251 (MQ=255)                                       ccAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGCGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCt                                                                                                                                                                                                              >  2:282697/1‑251 (MQ=255)                                           ttGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCCTTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGc                                                                                                                                                                                                            >  2:463377/1‑249 (MQ=255)                                            tGACCGCAGAATTCTTTTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCACCGGTAGTATTCGGGCGTCCAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATCCGGGATGTTG‑TCGTACGTCCCCGGTGCCGGAGGGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAg                                                                                                                                                                                                          <  1:687470/250‑1 (MQ=255)                                              cccGCAGAAGTCTTCTTAAATGCAAGGCCTTTTTTAAACAGTGCGGTGAAGAATTTGTGTCCCCAACGGTAGTATCCCGGCGTAAAGGTTGCCGGCCCGCGGCTCCAGTCA‑AACCAAAAG‑CCCAGAATTTTGAGCGGGTTTTTCA‑TATCCGCGATGTTG‑GCTTACGCCCACGGGGCGGGAGCGGGGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGAc                                                                                                                                                                                                       <  2:252292/250‑1 (MQ=255)                                                 gCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGGATTCCGGCGTACAGGGTGCCAGCTCGCGGCTCCAGGCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGCTGTTG‑TCGGACGTCCACGGTGACGGAGCGGTGTTGGTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGtt                                                                                                                                                                                                    >  2:392645/1‑251 (MQ=255)                                                   aGAAGTCTTCTTATATCCCAGGC‑TTTTTTATACAGCGCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCCCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGttttt                                                                                                                                                                                                 <  1:10090/251‑1 (MQ=255)                                                       gTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGcc                                                                                                                                                                                              >  1:505243/1‑251 (MQ=255)                                                          ttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGccc                                                                                                                                                                                             >  1:601950/1‑249 (MQ=255)                                                           tcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCCGGACGTTTGTGCCCAGc                                                                                                                                                                                          >  2:180239/1‑251 (MQ=255)                                                                 ataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGc                                                                                                                                                                                    >  1:525949/1‑251 (MQ=255)                                                                  acccAGGCTTTTTTTATACAGCCCGGTGAAGAATTTTTTTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCCCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGTTTTTTCA‑TATCCGCGATGTTG‑TCGTACGCCCACGGTGCCGGGGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCt                                                                                                                                                                                   <  1:228371/249‑1 (MQ=255)                                                                      aGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGt                                                                                                                                                                                >  1:314436/1‑250 (MQ=255)                                                                      aGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGAACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGAGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCACACGCGACCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCGGGt                                                                                                                                                                                >  2:207613/1‑250 (MQ=255)                                                                            tttttATACCGCCCGGTGAAGAATTTTTGTTCCCAACGGTAGTTTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTGGAGCTGGTTTTTCA‑TATACGCGATTTTG‑TCGTACGTCCACGGTGCCGGGGGGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCggg                                                                                                                                                                          <  1:51506/250‑1 (MQ=255)                                                                               ttATCCAGCTCGGTGAAGAATTTCTTTTCCCAACGGTAGTATTCCGGCTTACGGTTTGCCACCTCGCGGCTCCAGTCATAACCAAG‑C‑CCCAGCATTTTGGGCTGTTTTTTAA‑TAGCCGCGAT‑TTGCGTGTACGTCCTCGTTGCGGGGGCGGTTTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGa                                                                                                                                                                       <  1:456352/250‑1 (MQ=255)                                                                                 aaaCAGCCCGGTGAAGATTTTCTGTTCCCAACGGTAGTATCCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                                                                                                                     <  1:218723/248‑1 (MQ=255)                                                                                      gCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                                                                                                                                >  1:362922/1‑250 (MQ=255)                                                                                           gTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGAt                                                                                                                                                          >  1:24252/1‑251 (MQ=255)                                                                                           gTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTAAAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGAt                                                                                                                                                          >  1:280249/1‑251 (MQ=255)                                                                                                  aTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgtgtcg                                                                                                                                                   >  1:65771/1‑249 (MQ=255)                                                                                                        gTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                                                                                                                             >  1:652614/1‑251 (MQ=255)                                                                                                          ccccAACGGTAGTTTTCCGGCGTACGGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGCCCACGGTGCCGGGGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtac                                                                                                                                           <  1:18831/250‑1 (MQ=255)                                                                                                             cAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAACGGCTGCAGGACGTTTTTTCCCAGCATACGCTGGTAGCGGGCGATCACGTCCCCGATGGTGTAGTtacgtaagcg                                                                                                                                        >  2:716095/1‑247 (MQ=255)                                                                                                              aaCGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTgg                                                                                                                                       >  1:488584/1‑251 (MQ=255)                                                                                                                cGGTAGTATTCCGGCGTACAGGTTACCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGCGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTCGTTAAGTACGTGGcc                                                                                                                                     >  2:192946/1‑251 (MQ=255)                                                                                                                    agtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGCAGTTACGTACGTGGCCCAtg                                                                                                                                 >  2:472235/1‑251 (MQ=255)                                                                                                                                   aGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGa                                                                                                                   >  1:517606/1‑250 (MQ=255)                                                                                                                                     gTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑Agg                                                                                                               >  1:445388/1‑251 (MQ=255)                                                                                                                                       tGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCCCGTCACCGATGGTGGAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGAt                                                                                                             >  2:394203/1‑251 (MQ=255)                                                                                                                                       ggCCAGCTCGCGGCCCCAGTCATAACCAAA‑G‑CCCAGCATTTGGAGCTGGTTTTTCA‑TATACGCGATGTTG‑CCGTCCGCCCACGGTGCCGGAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGAt                                                                                                             <  1:650151/250‑1 (MQ=255)                                                                                                                                        gCCAGCTCGCGGCTCCCGTCATAACCAAG‑G‑CCCAGCATTTTGCGCTGGTTTTTCA‑TATACGCGATGTTA‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCCTACGCTGGTAGCGGGCGATCACGTCACCGGTGGTGGAGTTACGTACGGGGCCCATGGGTAATCGACCagg‑agg                                                                                                               >  2:209677/1‑248 (MQ=255)                                                                                                                                          cAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGCCCAGA‑AGGATAgg                                                                                                          >  2:181595/1‑251 (MQ=255)                                                                                                                                           aGCTCGCGGCTCCCGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCACCAGGACGGTTTTGCCCAGCATACGCCGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGGACAGGGCCCAGGTGCAGTCGCCCAGA‑AGGATAggg                                                                                                         >  2:488439/1‑251 (MQ=255)                                                                                                                                            gCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGCCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGCTAACGTCACCGATGGGGTAGTTACGTAAGAGACCCATGTGGAGACAACCACA‑AGGAtggga                                                                                                         >  1:127744/1‑246 (MQ=255)                                                                                                                                             cTCGCGTCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGAGATGTTG‑TCTTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCAAAGCCTTCCGCAGGCACACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGGTTTTTCACAGCATACGCTGGAAGCGGGCGATCACGTCACCGATGGTTTAGTTACGTACGTAGCGCATTTGTAATCGACC‑GACAGGATAGGGa                                                                                                        >  1:110755/1‑250 (MQ=255)                                                                                                                                             cTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGa                                                                                                        >  2:121105/1‑250 (MQ=255)                                                                                                                                                     cccAGTCATACCCAAA‑G‑CCCAGCATTTTGAGCTG‑TTTTTCAATATCGGCGAGGTTG‑TCGTACGTCCAGGGGGCCGGGGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGAc                                                                                               <  1:75574/250‑1 (MQ=255)                                                                                                                                                      ccagttaaaaCCAAA‑C‑CCCAGCTTTTTGGGCGGGTTTTCTA‑TATTCGCGATGTTT‑CCGTGCGCCCAGGGGGCGGGGGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGGCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGa                                                                                                <  2:574655/241‑1 (MQ=255)                                                                                                                                                       cAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGAc                                                                                               >  1:494372/1‑249 (MQ=255)                                                                                                                                                         atCATAACCAAA‑T‑CCCATCTTTTTGAGCTGGTTTTTCA‑TATCCGCTATTTTG‑TCTTACGCCCACGGTGCGGGAGCGGTTTTTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGc                                                                                           <  1:76485/250‑1 (MQ=255)                                                                                                                                                          gtacacccAAA‑GGCCCGGCATTTTGAGCTGTTTTTTAT‑TATCCGGGGTGTTG‑TCGTCCCCCCCCGGTGCCGGTGCGTTGTGTTTTTTCACCGCCCCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGc                                                                                           <  2:494372/245‑1 (MQ=255)                                                                                                                                                               aCCAAA‑G‑CCCAGCATTTTGGGCTGGTTTTTCA‑TATCCGCGTGGTTG‑TCGTACGTCCCCGGGGCCGGGGCGGTGTTTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAAt                                                                                    <  1:637674/251‑1 (MQ=255)                                                                                                                                                               aCCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAAt                                                                                    >  1:662321/1‑251 (MQ=255)                                                                                                                                                                 cAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGGAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGN‑AGGATAGGGAAGCATAGACAGGCAG‑TAATAc                                                                                  >  2:349629/1‑251 (MQ=255)                                                                                                                                                                    a‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTtc                                                                               >  1:166160/1‑251 (MQ=255)                                                                                                                                                                        cccagattgtggagtttgTTTTTTA‑TATACGCGAGTTTG‑TGGTACGTCCCCGGTTCCGGGGCGGTTTTTTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGTGAAGCATAGACAGGCAG‑TAATACTtctc                                                                             <  1:641413/234‑1 (MQ=255)                                                                                                                                                                        cccAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGANAGGGAAGCATAGACAGGCAG‑TAATACTtctc                                                                             >  2:358397/1‑251 (MQ=255)                                                                                                                                                                         ccAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTctct                                                                            >  1:118311/1‑251 (MQ=255)                                                                                                                                                                             gtatttggggtGGTTTTTCAATATCCGGGATGTTG‑CCTTCCGTCCAGGGGGCCGGGGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTg                                                                         <  1:352949/241‑1 (MQ=255)                                                                                                                                                                                 ttgggCTGGTTTTTCA‑TATCCGGGAGGTTT‑TCTTACGCCAACGGGGCGGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGctct                                                                     <  1:392645/246‑1 (MQ=255)                                                                                                                                                                                 ttGAGCTGGTTTTTCA‑TATCCGCGTTGTTG‑TCGTACGCCCACGGTGCGGGAGCGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGctct                                                                     <  1:444742/250‑1 (MQ=255)                                                                                                                                                                                       gggTTTTTCA‑TATACGCGATGTGG‑TCGTCCGCCCCGGGGGCGGGGGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTc                                                              <  1:282697/250‑1 (MQ=255)                                                                                                                                                                                       gggTTTTGCA‑TATACGCAAGTTTG‑TCGTACGTCCAGGGTGCCGGAGCGGGTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCtt                                                               <  1:503445/249‑1 (MQ=255)                                                                                                                                                                                        ggTTTTTCA‑GATACGCGATGTTG‑TCGTACGCCCACGGTGCCGGAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCg                                                             <  1:394203/251‑1 (MQ=255)                                                                                                                                                                                           ttttCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCACACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAg                                                                                             >  1:406009/1‑217 (MQ=255)                                                                                                                                                                                              tCA‑TATACGCGATGTTG‑TCGTACGTCCACGGAGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACt                                                       >  1:204373/1‑251 (MQ=255)                                                                                                                                                                                                cattcacggtagtttg‑tCTTACGCCCACGGNGCCGGAGCGGTTTTTTTTTCCACCGCCCCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACt                                                       <  2:366175/237‑1 (MQ=255)                                                                                                                                                                                                a‑tatACGCGATGTTG‑TCGTACGTACACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACTCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGGAGCGGGCGATCACGTCCACGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGCTAGGGAAGCCTAGTCAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTAcgtc                                                     >  2:709226/1‑248 (MQ=255)                                                                                                                                                                                                  tataCGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGGAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCCG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTc                                                     >  2:411611/1‑250 (MQ=255)                                                                                                                                                                                                         gATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAG‑CAGGAAATACTTCTTTTTGCTTCCGCCTTCGGTTACTTCAAAtgtg                                              >  1:496315/1‑250 (MQ=255)                                                                                                                                                                                                                   gTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtctcct                                    >  2:431055/1‑249 (MQ=255)                                                                                                                                                                                                                       gtccccgttgcgggggCGGTGTTGTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCa                                <  1:181595/236‑1 (MQ=255)                                                                                                                                                                                                                       gTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGGCCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑CAATACTTCTCTTTGCTCTAGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccc                                 >  2:171276/1‑250 (MQ=255)                                                                                                                                                                                                                       gTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccc                                 >  2:117089/1‑250 (MQ=255)                                                                                                                                                                                                                       gTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccc                                 >  1:645977/1‑250 (MQ=255)                                                                                                                                                                                                                        cccACGGTGCCGGGGCGGTGTGGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGCCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccc                                 <  1:457742/248‑1 (MQ=255)                                                                                                                                                                                                                           aCGGTGCCGGAGCGGCGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTTCGCTTCTCATCCCAATg                             >  1:139922/1‑250 (MQ=255)                                                                                                                                                                                                                            cGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGa                            >  1:289808/1‑250 (MQ=255)                                                                                                                                                                                                                             ggTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAACTGTGCGCTTCTCATCCCAATGa                            >  2:711995/1‑249 (MQ=255)                                                                                                                                                                                                                               tGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGCTAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCt                        >  1:297184/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggtggtgtattttCCCCGCCACTCCTTCCGCAGGCAGACCAGACGCGTCCCAGCCAATCGGCTGCATGTCGTTTTTGACCAGCATACTCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCACGTGTCGGCGACCAGA‑AGGATAGGGAAGCATCGACAGGCAT‑TAATACTTCTCTCTGCTCTCGTCTTCTGTTACTTCAAATGTGAGATTCTCATCTCAATGAAGCTGTACTTTg                <  2:375497/242‑1 (MQ=255)                                                                                                                                                                                                                                           gttgttTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt             <  1:231239/250‑1 (MQ=255)                                                                                                                                                                                                                                            ttgttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc           >  2:205165/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttgttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc           >  2:99493/1‑251 (MQ=255)                                                                                                                                                                                                                                                   tccccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtctc     <  1:716095/247‑1 (MQ=255)                                                                                                                                                                                                                                                     cccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTc  <  1:463377/250‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       ACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑G‑CCCAGCATTTTGAGCTGGTTTTTCA‑TATACGCGATGTTG‑TCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGA‑AGGATAGGGAAGCATAGACAGGCAG‑TAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674272‑674762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑