Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,027	T→G	intergenic (‑36/‑323)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,027	0	T	G	100.0%	150.4 / NA	46	intergenic (‑36/‑323)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/32);  total (14/32)

AGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATC  >  NC_000913/4312783‑4313273                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       agcTTTGATTCCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATcacc                                                                                                                                                                                                                                                  <  2:706176/251‑1 (MQ=255)              tAACTTTCAGCGCTTCAGATACCTCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCGGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCTCCACAGCAAGAAc                                                                                                                                                                                                                                       <  2:714927/249‑1 (MQ=255)                   atcatcGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGaaa                                                                                                                                                                                                                                  >  1:630442/1‑249 (MQ=255)                     catcGCTTCAGATACCTCTAGAGCTTCTGCGACATTGTTAAGTGCGGGTGCTCACCTCAGGTTACCGGGTTTGAGTAACCCCGCCACGATGCGTCTTTCAGTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGAt                                                                                                                                                                                                                              <  2:299875/251‑1 (MQ=255)                        cGCTTCAGATACCGCCAGAGCTTCTGCGACATCTCGAATTGCGGGAGCACAACTCAGGTTACCGGGTTTGAGGAACCACTCCAAGCTGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCTGTTAAGGCGCTATCCTTATACCGTTCTGAAGCGCTACATCAGACTCTCACTGGCTCATCACATTCTCTTGTCCTTGAATTGACGACGCCACTAGATAACCCAAGCAAGAACAGAAAAGATGCt                                                                                                                                                                                                                           >  2:527006/1‑251 (MQ=255)                          cTTCAGATACCGCCATATCCTCTGCGACAGCTTTAAGTTCGTGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCTCGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCTCCACAGCAAGAACAGAAAAGaggat                                                                                                                                                                                                                           <  2:546974/249‑5 (MQ=255)                            tCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTgg                                                                                                                                                                                                                         <  1:357811/249‑1 (MQ=255)                             cAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCg                                                                                                                                                                                                                       <  1:282146/250‑1 (MQ=255)                             cAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGACGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCg                                                                                                                                                                                                                       <  1:330807/250‑1 (MQ=255)                             cAGAAACCGCCAGAGCTTCTGCGACATCTTTAAATGCGGGTGCACAACTCAGGTTTCCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCg                                                                                                                                                                                                                       <  1:366227/250‑1 (MQ=255)                                atcccGCCCGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCTCGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACtt                                                                                                                                                                                                                   <  2:170577/248‑1 (MQ=255)                                  tccGCCAGGTCTTCTTCGTCATCCTTAATTGCGGGTGCACAACTCAGGTTCCCGGGTCTGAGTAACCACTCCTCGGTGCGGCTTTCAGTTGCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATTCCGTTCGGAAGCGCTGAATCAAACGCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCTCTAGATCACCACAGCAAGAACAGAAAATAAGCTGGCGTCTTc                                                                                                                                                                                                                  <  2:430636/249‑1 (MQ=255)                                  aCCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAATAACAGAAAAGATGCTGGCGACTTc                                                                                                                                                                                                                  <  1:208830/250‑1 (MQ=255)                                  aCCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTca                                                                                                                                                                                                                 <  1:309616/251‑1 (MQ=255)                                     gCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACaa                                                                                                                                                                                                              <  1:693244/251‑1 (MQ=255)                                                 gCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaataaaa                                                                                                                                                                                                    <  1:161406/249‑1 (MQ=255)                                                  cGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAAttt                                                                                                                                                                                                 >  1:551601/1‑251 (MQ=255)                                                          ttAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATaa                                                                                                                                                                                             <  1:160521/247‑1 (MQ=255)                                                               tGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttattt                                                                                                                                                                                    >  2:689567/1‑251 (MQ=255)                                                                  gggttcactactctttttACCGGGTTTTCGTAACCCATCCACGATGCGGCTGTCATGTTCTGTCAGTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACACTATACCGTTCGGAAGCGCTGAATCAAACACTGACTGGCTCATCCAGCTCTCTTGTCCTGGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAATAAAATAAAATTTATAAAGtttattttat                                                                                                                                                                                 <  2:509113/235‑1 (MQ=255)                                                                  gggTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGTTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGAAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttattttat                                                                                                                                                                                 >  1:404192/1‑251 (MQ=255)                                                                    gTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCGTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATtg                                                                                                                                                                               <  2:630442/251‑1 (MQ=255)                                                                           aCTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACaa                                                                                                                                                                        <  2:75642/251‑1 (MQ=255)                                                                                   tACCGGGGTTGAGGAGCCACTGCACGAGACGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCCTTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGAAATAACAGAAAAGATGCTTGCGACTTCACAAAAAAAGAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAg                                                                                                                                                                 <  2:90080/250‑1 (MQ=255)                                                                                              cgTAACAACTCCACGATGCGGCTTTCATTTTATGTCATTCCTTCCTACTTCATTCGCAGGTAAGGCGATAACCCTATACCGTTCGGAAGCGATGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTCGATCACCCCAGGAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGATTATGTTATTGTGAACAATGGAGAGTGGCTTAAAAtt                                                                                                                                                     >  2:635450/2‑251 (MQ=255)                                                                                              aGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtt                                                                                                                                                     <  1:320328/251‑1 (MQ=255)                                                                                               gTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAttt                                                                                                                                                    >  1:128886/1‑251 (MQ=255)                                                                                                     aCTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTNAACAATGGCGAGTGGCTTAAAATTTTATTTc                                                                                                                                              >  2:345532/1‑251 (MQ=255)                                                                                                      cTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCCTTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCg                                                                                                                                                                                                                       >  1:622570/1‑177 (MQ=255)                                                                                                      cTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCg                                                                                                                                                                                                                       <  2:622570/177‑1 (MQ=255)                                                                                                         cACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAttt                                                                                                                                          >  1:320407/1‑251 (MQ=255)                                                                                                                           ttCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACat                                                                                                                         <  1:556225/250‑1 (MQ=255)                                                                                                                                    tccttcctGCTTCAGTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAtt                                                                                                                <  2:714790/250‑1 (MQ=255)                                                                                                                                    cccatcctGCTGCATTCTCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTTAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCTCTAGATCACCACAGCAAGTACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAAATTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAttt                                                                                                               <  1:3136/247‑1 (MQ=255)                                                                                                                                     ccttcctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCNTTTAATTactccagcggctctaacgggtc                                                                                                                >  2:355470/1‑228 (MQ=255)                                                                                                                                        tcctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGt                                                                                                             <  1:236320/249‑1 (MQ=255)                                                                                                                                            gCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTc                                                                                                        <  1:513221/250‑1 (MQ=255)                                                                                                                                                    cGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGGCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt                                                                                               >  1:16360/1‑251 (MQ=255)                                                                                                                                                           aaGGCGCTAACACTATACCGGTCGGCAGCGCTGACTCACACTATGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACGCGATCACCACAGCAAGCCCAGAAAAGAAGCAGGCGACTTCACAACAAAATAAAATTTATAAAGTTTGTTATATTGTGAACAATGGCGAGTGGCATAAAATTTTATTTCATTTAATTAGTTTAAAAATATGCAGTTATTTGTTTTTCTGGTTTTTTAgatatt                                                                                        >  2:427636/1‑248 (MQ=255)                                                                                                                                                                                        gcTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTATATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTaa                                                           <  2:5346/251‑1 (MQ=255)                                                                                                                                                                                                     ctGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTgcgc                                                <  1:17571/249‑1 (MQ=255)                                                                                                                                                                                                           gggtcatcaCGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACATCAAGAACAGAATAGATTCTGGTGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTTGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACTCTATTGCGCTGTCAGag                                        <  2:659688/248‑2 (MQ=255)                                                                                                                                                                                                                   aCGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAATATCATGATGTTATGTGTTTTTCTGGTTGGTGAGATGATAGATTTTGCTGGATGGTGAGTGTCTTTACCGCTCTTGCGCTGTCAGATGTAActct                                <  2:551601/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggtgACGCAACTAGATCACCACAGCAAGCACAGAAAAGATGCTGGCAACTTAACAAAAAAATAAAATTTAAAAAGATTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGGTGTTAGATGTTGCTGGATGGTGAGTATCTTTAACGCTATTGCGCTGTACGGTGTAACTATGTAAAAAAGATCAGGAAGGCg           >  2:544524/4‑251 (MQ=255)                                                                                                                                                                                                                                                 aCTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATc  <  1:353410/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aCTAGATCACAACAACAAGAACAGAAAAGATGCTGGCTACTTCACAAAAAAATAAAATGTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATGTTATTTCATTTAATGAGTTTAAAAACATGATGTTATTTGTTTTGCTGGTTTTTTAGATGATAGATGTTGTTGGATGGTGAGTGTCTGTAACGCTATTGTGCTGTCAGATTTAATTCTGTAAATCAGATCAGGaatggttaattcagc  <  2:190139/251‑14 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       AGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATC  >  NC_000913/4312783‑4313273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑