Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,289	A→G	Y235H (TAT→CAT)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,289	0	A	G	100.0%	185.8 / NA	54	Y235H (TAT→CAT)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

ATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATT  >  NC_000913/4312041‑4312535                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       gtcagttttgacgtgaGGTAAGGGGACA‑GTANCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATc                                                                                                                                                                                                                                                     <  2:323208‑M2/235‑1 (MQ=255) gtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATc                                                                                                                                                                                                                                                     <  1:606393‑M2/235‑1 (MQ=255)   cagttttgacgtggGGTAAGGGGGCA‑GTAACTCCGCGATGGAGGAATTATCTTGTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGATGCATTACGACCTAATAACGGCGTTTCCGGGGGTGGTGAGCAAATAATATGATCg                                                                                                                                                                                                                                                    <  2:68849‑M2/236‑1 (MQ=255)    agttttgccgtgaGGTAAGGGGACA‑GTAAATCCGCGATGGCGGACTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGCAATCAACAATTGCGCCGGGTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGCGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATCTTCTTGCCGAGGCCTTCCGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGc                                                                                                                                                                                                                                                  >  2:602281‑M2/14‑251 (MQ=255)             gtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGTCTGGTGAGCAAATAATATGATCGGCCAGTTTCGc                                                                                                                                                                                                                                         <  2:271645‑M2/247‑1 (MQ=255)                     aGGGGACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTg                                                                                                                                                                                                                                 <  2:194773/251‑1 (MQ=255)                                       cgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGtt                                                                                                                                                                                                                <  1:700577/251‑1 (MQ=255)                                         aTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAGGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCCTGTTCAATGTTTAGCTTGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGATGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGACTCGCTATCGGGTAATGGGAGCTATGGGTta                                                                                                                                                                                                               >  2:176179/1‑250 (MQ=255)                                                    aCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCt                                                                                                                                                                                                   >  1:243582/1‑251 (MQ=255)                                                       tttttCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTg                                                                                                                                                                                                >  1:331775/1‑251 (MQ=255)                                                        ttttCTTATTTCAGCAAGCCTGGTGAGAAAAAATCAACAATTGCGCTGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGACCTGGGCGACAGAGACAAAAAAAGCGACCAGCCAGGTTGGCTGTAATATTATTGCCGAGGCATTACGACCTAATAACGGCGCTTCCGGGGCTGGTGAGGGAATAATATGATCGGCCAGTTTCGCTATCGTGGAATGGTTGCACTGGGTTATACAAATAATCTTTGc                                                                                                                                                                                               >  2:86789/1‑251 (MQ=255)                                                              tATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGcccc                                                                                                                                                                                            <  2:477165/248‑1 (MQ=255)                                                                 ttCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGAAAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACATAATAACGGCGTTTCCGGGGCTGGGGAGCAAATAATATGATCGGCCAGTTTCGCTATCGCAGAATGGTCGCTATGGTTTATACAAATACTCTTTGCCCCGTTCtt                                                                                                                                                                                      >  2:285952/1‑251 (MQ=255)                                                                   cAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCtttt                                                                                                                                                                                    <  1:404224/251‑1 (MQ=255)                                                                        cgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGcc                                                                                                                                                                               >  1:250840/1‑251 (MQ=255)                                                                          ccTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAg                                                                                                                                                                             <  2:22545/251‑1 (MQ=255)                                                                             ggTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCATTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTc                                                                                                                                                                          >  2:512667/1‑251 (MQ=255)                                                                                gagaAGAAATCAACAATTGCGCCGGTTTTTTCCATATTTATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTa                                                                                                                                                                        <  1:365421/250‑1 (MQ=255)                                                                                         acaacaaTTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCttt                                                                                                                                                              <  1:229326/250‑1 (MQ=255)                                                                                          caacaaTTGCGCCGGTTTTTTCCATATTAATAGTAGCTTGTTCAATTTTGAGCTGGGCGACAGAGACAAAAAAAGCTTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTTAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTGCTTTTTTGCCAGTTCTACGGCCGCttt                                                                                                                                                              <  2:478076/250‑1 (MQ=255)                                                                                          aaacaaGTGCGCCGGTTTGTTCAATATTAATATTAGATTGTTCAATGTTGAGCTGGGCGACAGGGACAAAAAAAGCGTCAAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGTCCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCATTTTCGCTATCGTTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCACGTTATTTGTTGCCAGTGCTACGCCCGCttt                                                                                                                                                              <  2:478067/249‑1 (MQ=255)                                                                                                  gcgcCGGTTTTTTCCATATTAATATTAGCTTGTTCCATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGGGAATGGTAGCTATGGGTTATACAATTAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATTACt                                                                                                                                                     >  2:128969/1‑251 (MQ=255)                                                                                                  gcgcCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACt                                                                                                                                                     >  2:278481/1‑251 (MQ=255)                                                                                                      cGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTGGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGtt                                                                                                                                                 <  1:469373/251‑1 (MQ=255)                                                                                                             taccATATTAATATTAGCGTTTTCAATGTTGAGCTGTGCGACAGAGACAAAAAAAGCGTCCAGCAAGGATGGTTGTAATATTCTTGCCGATGCAGTACGACATATTAACGGCGTTTCCGGGGGTGGTGGGCTAATAAGATGAACGTCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATACTCTTTGCCCCGTTCTTTTTTGCCAGGTCTACGGCCGCTTTTTCATCTCTGGTTCGCCCg                                                                                                                                           <  2:414343/247‑1 (MQ=255)                                                                                                               ccATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCGAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGa                                                                                                                                         <  2:550641/249‑1 (MQ=255)                                                                                                                                            aGCTGGGCGCCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCCAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATCCAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCCCAACATCtccttc                                                                                                           >  2:423668/1‑251 (MQ=255)                                                                                                                                                           ccAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAAGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTCCCAGCACAACATCTCCTTCCTGTAACAACGAAgg                                                                                            >  2:374463/2‑250 (MQ=255)                                                                                                                                                           aCAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGc                                                                                            <  1:683135/251‑1 (MQ=255)                                                                                                                                                              aaaaagGCGTCAAGCAAGGTTTGTTGTAATATTCTGGCCGAGGCATTACGACCTAATAACGGCGTTTACGGGGCTTGTGAGCAAATAATATGATCGGCCAGTTTCGCTAGCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTTCGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCAGTACCAGCACAACATCTCCTTCCTGTAACAACGCAGCGGa                                                                                         <  2:545200/251‑1 (MQ=255)                                                                                                                                                               aaaaaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAc                                                                                        <  1:107767/251‑1 (MQ=255)                                                                                                                                                               aaaaaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAc                                                                                        >  1:662023/1‑251 (MQ=255)                                                                                                                                                                 aaaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCAGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAAAAACGAAGCGGAcat                                                                                      >  1:619406/1‑251 (MQ=255)                                                                                                                                                                 aaaGCGTCCAGCAAGGTTGGGTGTAATATTCTTGCCGAGCCATTACGACCTAATAAAGGCGTTTCCGGTGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAAAAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCAATGGTTCGCCCGGAATGGGTCACTACCCGCACAACATCTCCTTCCTGTAACAACGAAGAGGAcat                                                                                      >  2:466158/1‑251 (MQ=255)                                                                                                                                                                      gTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatca                                                                                 <  1:607727/251‑1 (MQ=255)                                                                                                                                                                       tCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat                                                                                >  1:461131/1‑251 (MQ=255)                                                                                                                                                                        ccAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGTGCTGGTGAGCAAATAATAGGCTCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAACTAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGATCGCCCGGAATGGGTCACTACCAGCACCACATCTCCTTCCTGTAACAACGAAGCGGAcatctacatg                                                                               >  2:13547/1‑245 (MQ=255)                                                                                                                                                                                        tAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                               >  1:6968/1‑251 (MQ=255)                                                                                                                                                                                                 tGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCGATCGGTGAATGGTCGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCt                                                         <  2:618162/248‑1 (MQ=255)                                                                                                                                                                                                  gCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCNTCCTGATGTGAGCCTCAGGATAGGCCTGAc                                                      >  2:329318/1‑250 (MQ=255)                                                                                                                                                                                                      aGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGCCAGCGt                                                 >  1:545169/1‑251 (MQ=255)                                                                                                                                                                                                        gCATTACGACCTCATAACGGCGTTTCCGGGGNTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTAc                                               <  2:331775/251‑1 (MQ=255)                                                                                                                                                                                                               gACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGAGCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATg                                        <  1:285952/251‑1 (MQ=255)                                                                                                                                                                                                                aCCTAATAACGGCGTTTCCGGGGCTGGGGAGCACATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGGCACTACCAGCACAACATCTCCTTCCTGTACCAACGAAGCGGACCTCATCATGATGAGAGCATCAGGATAGTCCTGACAGCGTACTCCAATgg                                       >  2:236320/1‑250 (MQ=255)                                                                                                                                                                                                                         cGGCGTTTCCGGGGCTTGTGAGCAAATAATCTGATCGGCAAGTTTCGCAATCGGCGAATGGTAGCTATGGGTTATACAAATACTCTTTGCACCGTTCTTTTTTGCCAGTTCTCCGCCCGCTTTTACATCACTGGTTCGCCCGGCATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGCCATCATCATGATGTGAGCATCCGGATAGACCTGACCGCGGACGCCAATGGGCACGAACt                              >  2:638197/1‑251 (MQ=255)                                                                                                                                                                                                                          ggCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACtt                             >  2:294960/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggggCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTAc                    >  1:513463/1‑251 (MQ=255)                                                                                                                                                                                                                                    gggCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAAATTGAGCTGTAc                    >  1:643448/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGACAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACAt                  >  1:279126/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGCATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                               >  2:314182/1‑204 (MQ=255)                                                                                                                                                                                                                                       cTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                               <  1:314182/204‑1 (MQ=255)                                                                                                                                                                                                                                            gAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTGCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGcac            <  2:133314/250‑1 (MQ=255)                                                                                                                                                                                                                                               cAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGcac            >  1:628439/1‑247 (MQ=255)                                                                                                                                                                                                                                                aaaTAATATGCTCGGCCAGTTTCGCTCTCGGTGAATGGTAGCTATGGGGTATACAAATAAGCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGTAATGGGTCACTCCCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGGCATCATCATGATGTTAGCTTCAGGATAGGCCTGACATCGTACTCCAATGAGCAATAACTGGTGCTGTCCATCAGTACAtata       <  2:667203/251‑1 (MQ=255)                                                                                                                                                                                                                                                     atatgatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCGCAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCAtt  >  1:21907/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       ATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATT  >  NC_000913/4312041‑4312535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑