Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	535,337	T→C	A474A (GCT→GCC)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	535,337	0	T	C	98.8%	302.5 / NA	83	A474A (GCT→GCC)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (36/46);  minor base A (0/1);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCG  >  NC_000913/535093‑535581                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ttggcttGTCAAAAACCGGTGCGGCACAATGGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCt                                                                                                                                                                                                                                                 <  2:647138/244‑1 (MQ=255)    gtcggTCACAAATCGTGGCGGCAAAAATGCGNCGTGTCTTTAAAGCCCGCCACTGGATCAACTGTGGGCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTgggg                                                                                                                                                                                                                                             <  2:367006/247‑1 (MQ=255)               aTCGCTGCGGCACAAATGATGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGTTCCTGCTGGTCCGTTAGGCTGGACGATTCCGGCGGCGCTATGGGTTTGTGCCGCTGATCCGAAACGCCATTTGGTAGCGATTTCTGGCGCCTATGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCTTACCTCCATGTTCTGGTCAACAAAGCCTATCTggggattcttcgac                                                                                                                                                                                                                                   >  2:725509/1‑240 (MQ=255)                       ggaaaaatggcttcATGTTTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTTATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACAGCCATGTGCTGGTCAACAAGGCCTATCTGGGGCTGATTCGTCAGTCACAAc                                                                                                                                                                                                                          <  1:632075/238‑1 (MQ=255)                       ggCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAc                                                                                                                                                                                                                          >  1:161439/1‑251 (MQ=255)                            aatgggtgcattgcTTTAAAGCCCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTTATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCt                                                                                                                                                                                                                     <  2:430804/238‑1 (MQ=255)                            aaatgtggcATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCt                                                                                                                                                                                                                     <  1:479791/244‑1 (MQ=255)                             aaTGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTANCTGGGGCTGATTCGTCAGTCACAACGCGCtt                                                                                                                                                                                                                    >  2:334038/1‑251 (MQ=255)                              aTGCTGCTTGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCttt                                                                                                                                                                                                                   <  1:203901/251‑1 (MQ=255)                                gcgtcATGTCTTTAAGGACCGCCACTGGAGCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                                                                                                                                                 <  2:490239/247‑1 (MQ=255)                                    cATGTCTTTAAAGACCGCCCCTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACAt                                                                                                                                                                                                             >  1:541679/1‑251 (MQ=255)                                     aTGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAAAATTCCGTACATCCCTGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATg                                                                                                                                                                                                            >  2:571634/1‑251 (MQ=255)                                           ttAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGacta                                                                                                                                                                                                       <  1:71890/250‑1 (MQ=255)                                           ttAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGac                                                                                                                                                                                                         <  1:484265/248‑1 (MQ=255)                                            aaaacaCCGCCGCTGGATCAACTGTTGGCAGGCTGGTCCGTTAGGCTGGACGATTGCGGCGGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAAGGTTGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTTATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACTTGCCGTACATCCATGTGCTGGTCATCAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCTCTTTTGACATGGgctact                                                                                                                                                                                                     <  2:599688/246‑1 (MQ=255)                                               aGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCg                                                                                                                                                                                                  <  1:487357/251‑1 (MQ=255)                                                 aCCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTg                                                                                                                                                                                                >  1:716813/1‑251 (MQ=255)                                                       aCTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTc                                                                                                                                                                                          >  2:386375/1‑251 (MQ=255)                                                           gATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCtt                                                                                                                                                                                      <  1:672099/251‑1 (MQ=255)                                                              cAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTc                                                                                                                                                                                    <  1:67868/250‑1 (MQ=255)                                                                   ggggTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGaa                                                                                                                                                                               <  2:445356/248‑1 (MQ=255)                                                                        cAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCa                                                                                                                                                                          >  1:345413/1‑250 (MQ=255)                                                                           gCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGCCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTc                                                                                                                                                                      >  1:393456/1‑251 (MQ=255)                                                                                      aGGCTGGAGGATTCCGGCTGCGCTAGGGGTGTGTGCCGCTGATCCGAAACGCAAGGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaagtg                                                                                                                                                            <  2:17714/250‑1 (MQ=255)                                                                                      aGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaagtg                                                                                                                                                            <  1:132386/250‑1 (MQ=255)                                                                                       ggCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAgtgaagtgaa                                                                                                                                                          <  1:337430/251‑1 (MQ=255)                                                                                           ggACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGc                                                                                                                                                      <  1:263652/251‑1 (MQ=255)                                                                                               gATTCCGGCTGCGCTAGGGGTTTGGGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCGACg                                                                                                                                                  >  2:516165/1‑251 (MQ=255)                                                                                                aTTCCGGCTGGGCTAGGTCTGTGTTCCGCTGCTCCGAAACGCAATGTGGTGGCGCTTTATGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGATATTGGCGCGCGGTTCAACATTCCGTACATCCATGTGCTGGTACACAACGCCTATATGGGGCTGATACGTGAGTCACAACGCGCTTTTGAAATGGACTACTGCGTGCAACTCGCTTTAGAGAATATCAACTCCAGAGAAGTGACTGGCTACgg                                                                                                                                                 >  2:81826/1‑251 (MQ=255)                                                                                                  tCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGgt                                                                                                                                                <  1:643332/250‑1 (MQ=255)                                                                                                    cGGCTTCGCTAGGGGGTTGTGCCGCTGATCCGAAGCGCAATGTGGGTGCGATTTCTGGCGACTTTGACTTCCATTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTGCAACATTCCTTACATCCATGTGCTGGTCATCATCGCCTATCTGGGGCTGATTCGTCAGTCTCAACGCGCTTTTGACAGGGACTAGTGCGTGCAAATCGCTTTCGAGAATATGAACTCCAGTGAAGTTAATGGCTACGGTGtt                                                                                                                                             <  2:459745/251‑1 (MQ=255)                                                                                                                 ggTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAg                                                                                                                                <  1:701858/251‑1 (MQ=255)                                                                                                                  gTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGt                                                                                                                               >  1:682732/1‑251 (MQ=255)                                                                                                                        gCCGCTGATTCCAAACGCAATGTGGTGTCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCTCAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGGGAATGGCTACGGTGTTGACCACGTAAAAGTAGCgg                                                                                                                          <  2:387842/250‑1 (MQ=255)                                                                                                                         ccGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGa                                                                                                                         >  1:625520/1‑250 (MQ=255)                                                                                                                           gCTGATCCGAAAGGCAATGTGGTGGAGATTTCTGGCGACTTTGACTTCCCGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCGTGTGCGGGTCAACAACGCCGATCTGGGGCTGATTCGTCAGTCACAACGAGCATTTGCCATGGACTCCTACGTGCAACACGCTTTCGAGAATATCAACACCAGTGACGGGAATGGCNACGGTGTAGACCAAGTAAAAGTAGCGGAAgg                                                                                                                      >  2:350286/1‑251 (MQ=255)                                                                                                                              gATCCGTAACGCCATGTGGTGGCTATTTCTGGAGACTTTGGCTTCCCGTTCCTGGTTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACAGCCATGTGCTGGTCAACAAAGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACACGCTTTCGAGAATATCAACTCCAGTGAAGTGAAGGGCTACGGTGTTGACCACGTAAAATTAGCGGAATGttt                                                                                                                   <  2:683856/251‑1 (MQ=255)                                                                                                                              gATCCGAAACGTAATGTGGTGGCGATTTGTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGGGAAGTGAATGGCTACGGTTTTGTCCACGTAAAAGTAGCGGAAGGtt                                                                                                                    <  2:102455/250‑1 (MQ=255)                                                                                                                              gATCCGAAACGCAATGTTGTGGCGATTTCTGGCGACTGTGACTGTCAGTTCCTGATTGAAGAATTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGGGCAGGTCAACGACGCCGATCTGGGGCTGATTCGTCAGTCACATCGCGCTGTTGATATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTTTTTGGCTACGGTGTTGACCACGCAAAAGTAGTGGAAGAttt                                                                                                                   <  2:612171/251‑1 (MQ=255)                                                                                                                              gATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGGTACGGTGTTGACCACGTAAAAGTAGCGGAAGGttt                                                                                                                   <  1:198640/251‑1 (MQ=255)                                                                                                                              gATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGttt                                                                                                                   >  1:628902/1‑251 (MQ=255)                                                                                                                                     aaCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGt                                                                                                            >  1:487346/1‑251 (MQ=255)                                                                                                                                     aaCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGt                                                                                                            >  2:265906/1‑251 (MQ=255)                                                                                                                                       cGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTaa                                                                                                          <  1:60816/251‑1 (MQ=255)                                                                                                                                        gcactgtgGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGCGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACACCGCGCTTTTGCCATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGACGTGAATGGATACGGTGTTGGCCNCGTAAAAGTAGCGGTAGGTTTGGGTTGTaa                                                                                                          >  2:337982/5‑250 (MQ=255)                                                                                                                                        gCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTaaa                                                                                                         <  1:386375/251‑1 (MQ=255)                                                                                                                                         cAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAg                                                                                                        <  1:265906/251‑1 (MQ=255)                                                                                                                                           aTGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGACTATCAACTCCAGTGAAGTGAATGGCTACGgt                                                                                                                                                >  1:124424/1‑209 (MQ=255)                                                                                                                                           aTGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGgt                                                                                                                                                <  2:124424/209‑1 (MQ=255)                                                                                                                                               ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTc                                                                                                  <  1:239812/251‑1 (MQ=255)                                                                                                                                               ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGGTAGCTGGTGGCGCGCAGTTCAACATTCCGTACATCCATGTTCTTGTCAACAACGACTATCTGGTGCTGATTAGTCAGTCACAACGCGCTTTTGACATGGACTACTGCTTGCAACTAGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCGAtt                                                                                                   <  2:526746/250‑1 (MQ=255)                                                                                                                                                  ggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCggg                                                                                               >  1:627949/1‑251 (MQ=255)                                                                                                                                                   gcgctgtacggCGACTTTGACTTCAAGTTACTGATTGACGAGATAGATGTTGACGCGCAGTTCAACATTCCGTACATCAATGAGCTGGCCAATCACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAAGTCGCTTTCGTGAATATCAACTCCAGTGAAGTGAATGGCTACGAGGTTGAACAAGTAAAAGAAGCGGAAGGTTTAGGTTGTACAGCTGTTCgggg                                                                                              >  2:626710/10‑250 (MQ=255)                                                                                                                                                    cGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTc                                                                                             <  1:662992/251‑1 (MQ=255)                                                                                                                                                     gATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCt                                                                                            >  1:200100/1‑251 (MQ=255)                                                                                                                                                              cgccgttGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTGCGTTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCgg                                                                                   <  1:528387/246‑1 (MQ=255)                                                                                                                                                                    tGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGat                                                                              >  2:272183/1‑250 (MQ=255)                                                                                                                                                                           cAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGCAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGACAATATTGCGcc                                                                      >  2:304602/1‑251 (MQ=255)                                                                                                                                                                             gTTCCTGATTGCAGAGTTAGCTGTTGGCGAGCAGTTCAACATTCCGTACATCCATATGCTGGTCACCAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCCGTGAAGTGAATGGCTACGGCGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTCAAGCTCTTCGGGTCTTCAAACCCGCCGATATTGCGcc                                                                      >  2:43615/1‑249 (MQ=255)                                                                                                                                                                                   gATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCtt                                                                <  2:628902/249‑1 (MQ=255)                                                                                                                                                                                   gATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCtt                                                                <  1:693471/249‑1 (MQ=255)                                                                                                                                                                                      tGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGaa                                                            <  2:487346/250‑1 (MQ=255)                                                                                                                                                                                      tGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGATGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAAc                                                           >  1:388271/1‑251 (MQ=255)                                                                                                                                                                                       gAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTGTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACACCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGACTGCCTTTGAAc                                                           >  2:34180/1‑250 (MQ=255)                                                                                                                                                                                        aaGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGCCCACGTCCAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAAc                                                           >  2:283126/1‑249 (MQ=255)                                                                                                                                                                                           agTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCg                                                      >  1:351218/1‑251 (MQ=255)                                                                                                                                                                                            gTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGAGCCAGCCTTTGAACAGGCg                                                      >  2:689299/1‑250 (MQ=255)                                                                                                                                                                                                gCTGTGGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGc                                                 <  1:693127/251‑1 (MQ=255)                                                                                                                                                                                                 cTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAAAAGGCGAAAGcc                                                >  2:7646/1‑251 (MQ=255)                                                                                                                                                                                                         gcgcAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCCGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGTCTACGGGGTTGACCACGTAAAAGTAGCGGAAGGGTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGCTATTGCGCCAGCCTTTTAACATGCTAAAGCCttcattgc                                        >  2:158782/1‑245 (MQ=255)                                                                                                                                                                                                              gTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAATCCGGAAGATATGGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAat                                   <  1:283126/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCATTCCGTACATCCATGTGCTGGTCAACACCGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAATTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTTCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCg                               <  1:368729/251‑1 (MQ=255)                                                                                                                                                                                                                        ccGTACATCCATGTGCTGGTCAACAACGCCTACCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTAc                          <  1:397062/250‑1 (MQ=255)                                                                                                                                                                                                                         cGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCg                        >  1:622654/1‑251 (MQ=255)                                                                                                                                                                                                                          gTACCTCCATGTGCGTGTCAACAACGCCTATCTGGGGCTGATTTGTCAGTCAAAACGCTCTTTTGACATGGACTACTGCGTGCAACTCGCTTGCGAGAATATCAACTCCAGTGAAGTGAATGTCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGGCCGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCgg                       <  2:38220/251‑1 (MQ=255)                                                                                                                                                                                                                          gTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCgg                       <  1:162013/251‑1 (MQ=255)                                                                                                                                                                                                                               cccATGTGCGGGTCGACAACGCATAGCGGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACGACTGCGTGCAACGCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTc                  <  2:146931/250‑1 (MQ=255)                                                                                                                                                                                                                                    gtgCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTgg              <  1:476732/250‑1 (MQ=255)                                                                                                                                                                                                                                        tGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGtt         >  1:429394/1‑251 (MQ=255)                                                                                                                                                                                                                                            caacaaCGCCTATCTGGGGCTGATTCGTCAGNCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATtc     <  2:362336/251‑1 (MQ=255)                                                                                                                                                                                                                                             aacaacGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGGCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATtct    >  2:448311/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccaacGCCTATCTGGGGCTTATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATtctc   <  1:587468/250‑1 (MQ=255)                                                                                                                                                                                                                                                 acGCCTATCTGGGGCTGATTCGTCATTCCCAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGCCGTGGAAGTTATtcccg  >  2:702874/1‑246 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     TTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCG  >  NC_000913/535093‑535581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑