Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,463	A→T	*247K (TAA→AAA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,463	0	A	T	100.0%	227.4 / NA	63	*247K (TAA→AAA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (32/31);  total (32/31)

ATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCA  >  NC_000913/155220‑155706                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aTAAACACGGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                                                                                                                                                                                                                              <  2:705146/251‑1 (MQ=255)          gACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATAGTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAcc                                                                                                                                                                                                                                      <  2:186893/250‑1 (MQ=255)                  aaaCAACCGGTTTAGCGTGGTTACCTTCACTTTACCGGCTAAGATCAATATTAAATGCGTTCTATCCCAGCAGGAAGGTATGGTCAGATTCAACGAGTTCAACACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACTTGTCGATACGGGTCAGATGATGATAATTTTTAGTATGTTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGTCTGATTTCAGccagtctttaccgtcaaatg                                                                                                                                                                                                                              <  2:294122/250‑19 (MQ=255)                    acacCCGGTATAGCGGTGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCATGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGCCATTACCTTCAATTgcgc                                                                                                                                                                                                                           <  2:606015/251‑1 (MQ=255)                      acCCGGTATAGCGGGGTTACCTTCACTGTACCTGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGcca                                                                                                                                                                                                                         <  1:58716/251‑1 (MQ=255)                      acCCGGTATAGCGGGGTTACCTTCACTATACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCCGGAAGGTATGGTCACATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGCTGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTTTATCCTTGCCTGCTTTCAGCCTGGCATTACCTTCATTTGCGccc                                                                                                                                                                                                                         >  2:86722/1‑250 (MQ=255)                          ggTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGCATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCACGCAAAACGTGGCGATACGGGCCAGATGATGATAATTTTTAGAATATTCTATAATCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                                                                                                                                                                                                      >  1:286352/1‑250 (MQ=255)                          ggTATAGCGGGGGTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCACAACGTGGCGATACTGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                                                                                                                                                                                                      >  2:59829/1‑250 (MQ=255)                           gTATAGCGGGGTTTCCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa                                                                                                                                                                                                                    <  1:54788/251‑1 (MQ=255)                                gtggggTGACCTTCACTGTACCGGCTGAGATCAATATTAGAGGCATTCTGCCCCAACAGGAAGGTATTGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCATCGCGCAAAACTTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCag                                                                                                                                                                                                               <  2:103115/249‑2 (MQ=255)                                     gTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGATTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg                                                                                                                                                                                                          <  2:643729/251‑1 (MQ=255)                                     gTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg                                                                                                                                                                                                          >  2:394041/1‑251 (MQ=255)                                       tACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCAGCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATgg                                                                                                                                                                                                         <  2:713940/250‑1 (MQ=255)                                                     cGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGCTACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt                                                                                                                                                                                          >  2:389924/1‑251 (MQ=255)                                                        cTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGAATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg                                                                                                                                                                                       >  1:256645/1‑251 (MQ=255)                                                         tGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGc                                                                                                                                                                                      >  1:42056/1‑251 (MQ=255)                                                          gagaTCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGc                                                                                                                                                                                      >  2:99355/1‑250 (MQ=255)                                                            gaTCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGc                                                                                                                                                                                      >  2:634270/1‑248 (MQ=255)                                                                 atatTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGCACGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGACTTCg                                                                                                                                                                              >  2:280947/1‑251 (MQ=255)                                                                                 gCCCCATAAAGAAGGTAAGGTCATATGCATCGAGGTCAGCCCTGAAAGCAGTATTGCAATACAGCAGCGCGTAAAACGGGGCGAGTCGGGTCAGATGATGATAATTTTTAGTATTTTATAGTTTCACGCGATGCTTCCTTCGGAATTATTTCCTTGCCTGATTTCAGCCTGGCAGTTCCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAACATGCACGTTTGCAGAATTCGCTGTGCCATGAAg                                                                                                                                                                 <  2:689199/248‑1 (MQ=255)                                                                                   cccATCAGGAAGTTATGGTCATATTCAACGAGGTCAGCACTGAAAGCAGTATGGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATAGTCTATAGTCACGCGATGCTTCCTGCGGAATGATATCCTTGCCTGATTTCATCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCAATTAAGGCCAt                                                                                                                                                            <  2:69606/251‑1 (MQ=255)                                                                                      aTCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATGAACGCCATTGa                                                                                                                                                         <  2:406642/251‑1 (MQ=255)                                                                                         aGGAAGGTAAGGGCATATTCAACGAGTTCAGCACTGAAAGCAGTATGGCAATACAGCAGCGCGCAAAACGTGGAGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGACACGCTAGGCTTCCGGCGGAATTATATCATTGCCTGATTTCAGCCTGGCATTACCTTCATTTGTGCCACCAAAGTCATTAATGGAGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGTCAATTGACtt                                                                                                                                                      >  2:408828/1‑251 (MQ=255)                                                                                          ggAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACttt                                                                                                                                                     >  1:158137/1‑251 (MQ=255)                                                                                                 aTGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGACACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAAGTCGCTTTGCCATTAAGGCCATTGACTTTCATGAc                                                                                                                                               <  2:206861/250‑1 (MQ=255)                                                                                                   ggacATATTCAACGAGCTCAGCACTGAGAGCAATATTGCAATACAACAGCGCGCAAAACGTGGCGATCCGGGTCAGTTGATGATAATTTTTAGCATATTCTATCGCCACGCTATGATTCATGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTCCCTTCAGTTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGTCCATCGACTTTCATCACCtc                                                                                                                                            >  2:237438/4‑251 (MQ=255)                                                                                                   ggTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCtc                                                                                                                                            <  1:412885/251‑1 (MQ=255)                                                                                                      cATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCTCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCtcat                                                                                                                                          <  2:115818/250‑1 (MQ=255)                                                                                                      cATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCtcat                                                                                                                                          <  1:337829/250‑1 (MQ=255)                                                                                                        tatTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCAc                                                                                                                                       >  1:38236/1‑251 (MQ=255)                                                                                                         atTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCAc                                                                                                                                       <  1:634270/250‑1 (MQ=255)                                                                                                                                  aGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCa                                                                                                             <  1:40651/251‑1 (MQ=255)                                                                                                                                        gCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGa                                                                                                       <  1:389924/251‑1 (MQ=255)                                                                                                                                             aCAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGc                                                                                                   <  1:280947/250‑1 (MQ=255)                                                                                                                                                  agcGCGCAAAACGGGGCGGTACGGGTCAGATGATTATAATTTTTAGTATATTCTCTAGTCACGCTATGCTTCCTGCGGACTTATCTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCAACCAAGTCATTAATGGCGTAAAAATGAACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCACATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTAc                                                                                             >  2:595107/1‑251 (MQ=255)                                                                                                                                                     gcgcAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGc                                                                                          >  1:145593/1‑251 (MQ=255)                                                                                                                                                         aaaaCGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGc                                                                                      >  2:276682/1‑251 (MQ=255)                                                                                                                                                         aaaaCGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGc                                                                                      <  1:206579/251‑1 (MQ=255)                                                                                                                                                              gTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCGGGCATTACCTTCAATTGCGCCACCAAAGTCATTACTGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACATCATCACAAAATGTGGCAATCATTTTCACATCAAGCGGATAGAGTTTACCGCAAGCTTCt                                                                                  >  2:137041/1‑250 (MQ=255)                                                                                                                                                                  ggATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATc                                                                             <  2:558778/250‑1 (MQ=255)                                                                                                                                                                  cGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATc                                                                             >  1:211429/1‑251 (MQ=255)                                                                                                                                                                    aTACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCttt                                                                                                                                                                          >  1:281479/1‑156 (MQ=255)                                                                                                                                                                       cGGGTCAGATGATTATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGTCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTGACCGCTAGCTTCTAAATctccac                                                                        <  2:53535/251‑1 (MQ=255)                                                                                                                                                                              gatgatggtAATTTTTAGTCTATTCTATAGTCACGCTATGCTTCCTGCGGTATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATCAATGGCGTAAAATTGCACTTTTTCAAAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAATTGGTGCAATCATTTTCACATCACTCGGCTATCTTTTACCGCTGGCTTCTAAATCACCACTGCTaac                                                                 >  2:10997/1‑250 (MQ=255)                                                                                                                                                                                tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGAGCAATCATTTTCACATCAATCGGATAGCGTTAACCGCTAGCTTCTAAATCACCACTGCTaaa                                                                 >  1:251822/1‑249 (MQ=255)                                                                                                                                                                                   tgatAATTTTTCGAATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAag                                                               >  1:557465/1‑248 (MQ=255)                                                                                                                                                                                    gatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTacctctttttgtcaagcaaa                                                              >  1:315541/1‑229 (MQ=255)                                                                                                                                                                                       aaTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGGAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACgt                                                         <  2:400008/250‑1 (MQ=255)                                                                                                                                                                                         tttttAGTATATTGTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATCGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAACAGAGACgtagt                                                      >  2:711225/1‑251 (MQ=255)                                                                                                                                                                                         tttttAGTATATTCTATAGTCACGCTATGGCTCCTGCGGAATTATATCCTTTCCTGATTTAAGCCTGGCATTCCCTTAAAGTGCGCCTCCAAAGTCCTTAATGGAGTAAAAATGCACTTTTGCAGAATTCGCTATGCCATTAAGGCCATTGTATTTGATGACCTCATCACTAAATGGAGCACTCATCTTCAAATCATTCGGATAGCGATTACCGCTCGCTTCTATATCACCACAGCTACAAGGGACgaagt                                                      >  2:45784/1‑251 (MQ=255)                                                                                                                                                                                           tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGc                                                                                                                             >  2:242347/1‑178 (MQ=255)                                                                                                                                                                                           tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGc                                                                                                                             <  1:242347/178‑1 (MQ=255)                                                                                                                                                                                              aGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAgg                                                  <  1:219412/250‑1 (MQ=255)                                                                                                                                                                                                  tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTg                                             >  2:304251/1‑251 (MQ=255)                                                                                                                                                                                                    ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTgg                                            >  1:484057/1‑250 (MQ=255)                                                                                                                                                                                                        ataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTaa                                                                                <  2:140038/210‑1 (MQ=255)                                                                                                                                                                                                        ataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTaa                                                                                >  1:140038/1‑210 (MQ=255)                                                                                                                                                                                                             cACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTTCTCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGAGAGCGTGTACCGCTAGCTGCTAAAGCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCAc                                   <  2:47622/250‑1 (MQ=255)                                                                                                                                                                                                             cACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACt                                  <  1:340763/251‑1 (MQ=255)                                                                                                                                                                                                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGaa          <  1:276682/250‑1 (MQ=255)                                                                                                                                                                                                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAAc         >  1:380687/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttGCCTGATTTCAGACTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTg      >  2:62335/1‑250 (MQ=255)                                                                                                                                                                                                                                             ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCa  <  1:489920/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCA  >  NC_000913/155220‑155706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑