Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	243.4 / NA	67	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (34/33);  total (34/33)

ATCTTCTTTTACCATCAACGTGTTAGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGT  >  NC_000913/3560205‑3560694                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                attttcttTTCCCACCAAGGTGTTAGCAA‑GTTGGAGTTTGTGGGTGACAAGGCGAAATTTGCTGTGATTGAGCGGTGGGTGCCCTCCCGCTTCCGGCGGGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCTTTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                  <  1:46296/248‑1 (MQ=255)                 aaCGTGTTAGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                  >  1:246085/1‑251 (MQ=255)                 aaCGTGTTAGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAAGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                  >  2:712830/1‑251 (MQ=255)                  aCGTGTTAGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                 >  2:91425/1‑251 (MQ=255)                  aCGTGTTAGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGATTTGc                                                                                                                                                                                                                                 >  1:618580/1‑251 (MQ=255)                         aGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                           >  1:556413/1‑250 (MQ=255)                            aaCGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                       >  1:652821/1‑251 (MQ=255)                             aCGTGG‑AGTTTGTGGGTGACAATGCACAATTTGCTGTGTTGGAGCGTTGCGTGCGCTCCCGCTCCGGGCGTGG‑GGCCG‑ATATCGAAAAACAGCGTCGAGCCTTTGGGGATTTGCGCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                                      <  1:65746/251‑1 (MQ=255)                                 tG‑AGTTTGTTGGTGACAATGCGCAATTTGTTGTGTTTGGGCAGTGCGTGCCCTCCCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAACCAGCGTCGGGCCTTTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                   <  1:191502/250‑1 (MQ=255)                                 gg‑agaTTGTTGGGGATAATGCGAAAATGGCTGTGATTGAGCGGTGCGTGCTCTCCCGCT‑CCGGGGTGGGTGCCA‑ATACCGATAACGAGGGTGGAGCCTTTGGGGTTTTGCCCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGTTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                   <  1:262319/249‑1 (MQ=255)                                           tgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTCCGCCACCCCATTCAGGTCgcgg                                                                                                                                                                                                         >  2:519987/1‑251 (MQ=255)                                           gggggacagtgggcAAATGGCTGTGATGGGGCAGTGCGGGCGCTCCCGTTTCGGGCGTGG‑‑GCCGGATATCGATAAACAGCGTCGAGCCATTGGGGTTTTGCGCC‑GCCACTTGG‑CGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg                                                                                                                                                                                                          <  1:604989/238‑1 (MQ=255)                                              tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                       >  1:717275/1‑250 (MQ=255)                                                aCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGCTTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                    >  1:201388/1‑251 (MQ=255)                                                   aTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                                 >  1:695484/1‑251 (MQ=255)                                                               cTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                                     >  1:534503/1‑251 (MQ=255)                                                                 gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                   >  2:151418/1‑251 (MQ=255)                                                                  tgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGCGGTCGCGGCGAATAGGCCGCGGGCTgcaggg                                                                                                                                                                                  >  2:677358/1‑246 (MQ=255)                                                                  tgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACggg                                                                                                                                                                                  >  1:474184/1‑250 (MQ=255)                                                                              gcgtgcgCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGCTTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                                                                                                                      >  1:596301/1‑251 (MQ=255)                                                                                  gcgcTACCGCTTCCGGCGTGG‑TGCCA‑ATACCGATAAACAGCTTCGAGCCTTTGGGGTTTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc                                                                                                                                                                  <  1:60007/251‑1 (MQ=255)                                                                                       ccccctcccGGCGTGG‑TCCCT‑ATCTCGAAAAACAGCGTCGGGCCTTGGGGGTTTTCCCCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                             <  1:101211/244‑1 (MQ=255)                                                                                         cGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                                                                                                                           >  1:85338/1‑251 (MQ=255)                                                                                             tCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCCGGATCAGGTTTTGCTCAGCCAGCTCATTGAGGTCGCGGCGTATAGTCTGCGGGCTGGCGGAGAGATGCTCTACCAGCTCTTCGGGACt                                                                                                                                                       >  2:269094/1‑251 (MQ=255)                                                                                                          ccG‑ATATCGATAAACAGCGTCGAGCCATTGGGGCTTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATTCTCTACCAGCTCTTCGGTACTGGCATAACCctt                                                                                                                                           >  2:174089/1‑250 (MQ=255)                                                                                                            g‑ATATCGATAAAAGGCGTGGAGCCTTGGGGGATGTGCCCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGTCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                                         <  1:186096/251‑1 (MQ=255)                                                                                                              atatCGATAAAAAGCGTCGAGCCATTGGGTATTTGATCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCATCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGACGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCACTACCAGCCATTCGGAAATGCCATAACCctgctt                                                                                                                                        >  2:28640/1‑250 (MQ=255)                                                                                                               tatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGCACTGCCATAACCCTGCTGt                                                                                                                                       >  2:394779/1‑251 (MQ=255)                                                                                                                  caaTAAACAGCGTCGAGCCTTTGGGGATTTGCCCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                    <  1:481033/249‑1 (MQ=255)                                                                                                                    aaaaacggcggtgggcctttGGGTATTTGCTGC‑GCC‑CTTTGGCGGGCGATGCGCTCTTTTTCTTGGGTTGGGGGGGCCTTGCGAGCGTCCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                  <  2:519123/233‑1 (MQ=255)                                                                                                                        acaccgtggacccgtggggtaTTTTCTCC‑GCCACTTTG‑CGGGCTATGCGCTCTTTTTCTTCGGGCGGGGGGGCTTTGCGATCGGGCCACGGCGGGTTAACCGAACGGGAAGGCA‑GGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                              <  1:305677/231‑1 (MQ=255)                                                                                                                        aCAGCGTCGAGCCTTTGGGGATTTGCTCC‑GCCACTTTG‑GGGGGGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                              <  1:556134/251‑1 (MQ=255)                                                                                                                           gtgTCGAGCCTTTGGGGTTTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                           <  1:531947/249‑1 (MQ=255)                                                                                                                           ccgtggacccatgggggtttggcccc‑GCCCCTTTG‑GGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                           <  1:519987/227‑1 (MQ=255)                                                                                                                              tCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCGat                                                                                                                         >  1:414409/1‑250 (MQ=255)                                                                                                                                         gggATTTGCCCCCGCCACTTTG‑CGGGCGATACGCTCTTTTTCTGCGGTCTGGTTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGAAAGGCG‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTATCCAGTTCGATAATACCGTtgt                                                                                                              <  1:226065/251‑1 (MQ=255)                                                                                                                                               gccccc‑GCCCCTTTG‑CGGGCGAGGCGCTCTTTTTCTGCGGGCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACGGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                        <  1:622088/246‑1 (MQ=255)                                                                                                                                                  gctccgctgtttggcgggggattctctctTTTTCGTCGGTGTGGGTGGCCTGGCGAGCGTGCCTCGGCGTTTTACCCTAAGTGAAAGTCA‑GGCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                                      <  2:591309/226‑1 (MQ=255)                                                                                                                                                       cctcttgg‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCAAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                <  1:258854/244‑1 (MQ=255)                                                                                                                                                            ttG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGTCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCTCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                            <  1:237655/250‑1 (MQ=255)                                                                                                                                                            tgg‑CGGGCGATGCGCTCTTTTTCTGCGGTCGGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                            <  2:293051/248‑1 (MQ=255)                                                                                                                                                                  ggCGATGCGCTTTTTTTCTTCGGTCTGGTGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCG‑GCGCCCCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                                                      <  1:80909/251‑1 (MQ=255)                                                                                                                                                                     gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTAGCTAATACCNTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                                   >  2:345268/1‑251 (MQ=255)                                                                                                                                                                             ctTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTgg                                                                             <  1:672930/249‑1 (MQ=255)                                                                                                                                                                              tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCAAGGAGTGTTAACCGACCTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCCTTGCGGTCGCGGCGCATAGTCCGCGGGCTGACGGCGAAAGGCTCTACCAGCTCTTCGGTACTGAAATAAACCTGCGGTATTACCAGTTCGATACTACCGGTGTGACGTTTTGTTTGTTTCCTTTAAAAATCCCTggcat                                                                          >  2:633268/1‑248 (MQ=255)                                                                                                                                                                                tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTAACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                                         >  2:657542/1‑250 (MQ=255)                                                                                                                                                                                 ttcttcgggtggggTGGCCTTGCGGTCGTGACACGGCGTGTAAACCGAAGTGGAAGGCATGC‑CGGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATATCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAatta                                                                        <  2:195252/239‑1 (MQ=255)                                                                                                                                                                                  gttcgtgtcggggTGGCCTTGCGATTGGGCCACGGGGTGTGAACCTAACTGGAAGGCA‑GAGCCGCCCCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACTCTGCTTTTTAACCAGTACGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                       <  2:20528/240‑1 (MQ=255)                                                                                                                                                                                      cGGTCTGTGTGGCCTTGCGATCGGGCCACTGGTTGTTAACCGAACAGAAAGGCA‑GCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttc                                                                   <  1:483645/250‑1 (MQ=255)                                                                                                                                                                                      cGGTCTGGGTGGCCTTGCGGTCGTGCCAGGCCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcg                                                                  <  1:269094/251‑1 (MQ=255)                                                                                                                                                                                       ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCTGTTCGATAATACCGTTGTGACGTTGTTTTTGTTTCATTTATTAAACCCTGGAATTAttttcgt                                                                 >  2:131777/1‑251 (MQ=255)                                                                                                                                                                                        gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                                >  1:640727/1‑251 (MQ=255)                                                                                                                                                                                               gggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGGTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                         <  1:81788/250‑1 (MQ=255)                                                                                                                                                                                                 gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCG‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAt                                                       <  1:299226/251‑1 (MQ=255)                                                                                                                                                                                                     tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCACACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCATCTCATTGAGGCCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGTTTAACCAGCTCGCTAATACCGCTGTGACGTTTTGTTTGTTTCATTTATAAATCCCTGGACTTATTTTCTGTTTCGCGCATTgcg                                                   >  2:642398/1‑249 (MQ=255)                                                                                                                                                                                                      gctgtCGTGCCACGGCGTGTTACCCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATGCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                   <  1:79325/246‑1 (MQ=255)                                                                                                                                                                                                         aTCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAACAGCTCTTCGGAACTGGCATAACCCTGCTGTTTAACCAGTTAGATAATACCGTTGTGACGTTGTGTTTGTTTCCTTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                                >  1:606687/1‑250 (MQ=255)                                                                                                                                                                                                                cACGGCGTGTTCACCGAAGTGGAAGGCG‑GCGACGCACCGCCATGATGGCGCAGGATAAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGAACTGCCATAACCCTGCTGGTTAAACCGTTCGATACTAACGTTGTGTCGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                         >  2:596118/1‑250 (MQ=255)                                                                                                                                                                                                                  gggggttttAACCGAACGGGACGGCG‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                      <  2:474184/244‑1 (MQ=255)                                                                                                                                                                                                                   ggCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGCCGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                       >  2:411019/1‑249 (MQ=255)                                                                                                                                                                                                                   ggCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                       >  1:541041/1‑249 (MQ=255)                                                                                                                                                                                                                      gtgtTAACCGACATGGGTGGCA‑GCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa                                  <  2:237715/251‑1 (MQ=255)                                                                                                                                                                                                                      gtgtTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                   >  1:342968/1‑250 (MQ=255)                                                                                                                                                                                                                              cGAACTGGAGGCCA‑GCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                          <  2:541041/251‑1 (MQ=255)                                                                                                                                                                                                                                cacctgtaagtcggctccgtaCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCGGCTGTTTAACCAGTTCGATAATTCCGTTGTGACGTTGTTTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc                         <  1:256870/231‑1 (MQ=255)                                                                                                                                                                                                                                 aCTGGAAGGCA‑GCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                       >  1:185857/1‑251 (MQ=255)                                                                                                                                                                                                                                                     ccGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgt    <  1:712830/251‑1 (MQ=255)                                                                                                                                                                                                                                                       gCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTCACCCGGCGAtgtgt  >  2:41352/1‑251 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ATCTTCTTTTACCATCAACGTGTTAGCAACGTTG‑AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGG‑TGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGT  >  NC_000913/3560205‑3560694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑