Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,426	Δ1 bp	intergenic (‑16/‑216)	yhiN ← / → pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,426	0	G	.	100.0%	326.1 / NA	69	intergenic (‑16/‑216)	yhiN/pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (27/42);  total (27/42)

AAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATT  >  NC_000913/3637181‑3637669                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cacgctgcatgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatggttgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaatggccactagcacacctgatccctctctaaataccctatccgaGTTGCGCTCCTCTTAaaaaaa                                                                                                                                                                                                                                                <  1:50619‑M2/21‑1 (MQ=255)   cgctgcatgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACg                                                                                                                                                                                                                                             <  2:239095‑M2/23‑1 (MQ=255)       gcatgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAacggac                                                                                                                                                                                                                                          <  2:247612‑M2/26‑3 (MQ=255)             gcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAatg                                                                                                                                                                                                                                   <  2:90554‑M2/33‑2 (MQ=255)                     accacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTaa                                                                                                                                                                                                                               >  1:231505‑M2/211‑247 (MQ=255)                          gacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTc                                                                                                                                                                                                                      >  1:330180‑M2/206‑251 (MQ=255)                             gactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGt                                                                                                                                                                                                                   >  2:246636‑M2/203‑251 (MQ=255)                             gactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGt                                                                                                                                                                                                                   >  1:310389‑M2/203‑251 (MQ=255)                                tgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagaattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGGCGTGATATGTAAAGATTCCTCAGTGGt                                                                                                                                                                                                                >  2:27717‑M2/200‑251 (MQ=255)                                     ggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCAc                                                                                                                                                                                                           <  1:246636‑M2/57‑1 (MQ=255)                                           cgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggttaacgatcaacaaagggccactagcacaccttattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACcatcat                                                                                                                                                                                                     <  1:283721‑M2/63‑1 (MQ=255)                                              cgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCaa                                                                                                                                                                                                  <  2:129249‑M2/66‑1 (MQ=255)                                               gtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaattccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAat                                                                                                                                                                                                 <  2:559916‑M2/67‑1 (MQ=255)                                               gtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAat                                                                                                                                                                                                 >  2:377015‑M2/185‑251 (MQ=255)                                               gtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAat                                                                                                                                                                                                 <  1:553044‑M2/67‑1 (MQ=255)                                                    gaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTAc                                                                                                                                                                                            <  1:720945‑M2/72‑1 (MQ=255)                                                      aaggttacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctagccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATACCTCAGTGGGCGCACCAGCATCAATATTACTa                                                                                                                                                                                          <  2:484681‑M2/74‑1 (MQ=255)                                                      aaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcactcctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGAGTCCTCAGTGGTCGCACCAGCATCAATATTACTa                                                                                                                                                                                          <  2:187678‑M2/74‑1 (MQ=255)                                                       aggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTa                                                                                                                                                                                          >  1:428164‑M2/177‑250 (MQ=255)                                                       aggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTa                                                                                                                                                                                          >  2:111318‑M2/177‑250 (MQ=255)                                                       aggggacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTaa                                                                                                                                                                                         <  2:411768‑M2/75‑1 (MQ=255)                                                        ggtgacatgggtggcgtgccgcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattatcgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACTGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTaaa                                                                                                                                                                                        <  1:231316‑M2/76‑1 (MQ=255)                                                         gtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTaaa                                                                                                                                                                                        >  1:150806‑M2/175‑250 (MQ=255)                                                           gacatgggcgacgtgcagcattttgacgacgacgttctcgcccagcgcggcgtgcgacacgcgcttttgcagtgcttgccgaaggaagattgtgtctattattgggtcctttggtgaccgatcaacaacgggccactagcacacctgattcctctctaaataccctatccgaGCTGCGCTCCTCTTA‑ACAAAACGGACGTGAATTGTAAAGATTCCTCAGTGCTCGCACCAGCATCACTATTACTAAAAgg                                                                                                                                                                                     >  1:585928‑M2/173‑251 (MQ=255)                                                                    gacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTAttt                                                                                                                                                                             <  1:662539‑M2/87‑1 (MQ=255)                                                                    gacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTg                                                                                                                                                                            >  1:374480‑M2/164‑251 (MQ=255)                                                                     acgtgcagcatgttgccgatgccgttatcgcccatctcggcgggcggcacgggcattttcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgacccatcaacaaagggccactagcacacctgattcctctctaaataccctatcctaGTTGCGCTCCTCGTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCCGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGc                                                                                                                                                                           <  2:481631‑M2/89‑1 (MQ=255)                                                                           agcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAAt                                                                                                                                                                     <  2:561428‑M2/95‑1 (MQ=255)                                                                              attttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATtat                                                                                                                                                                  >  1:242966‑M2/154‑251 (MQ=255)                                                                                   gccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATaa                                                                                                                                                              <  1:11336‑M2/102‑1 (MQ=255)                                                                                   gccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGAATTTGCCTGAATtatata                                                                                                                                                               >  1:495860‑M2/149‑249 (MQ=255)                                                                                      gatgacgttatcgccccgcgcggcgtgcggcacgggcctttgcagtgcttgccgaaggcagcttgagtctattattgggtcctttggtgaacgatcaacaacgggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AACAAACGGACGTGAAGTGTAACGATTCCTCAGTGGTCGCACCAGCATCATTCTTACTAACAGGAagtatttgcctgactttaatacgat                                                                                                                                                           >  2:134114‑M2/146‑238 (MQ=255)                                                                                      gatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATa                                                                                                                                                          <  1:141741‑M2/106‑1 (MQ=255)                                                                                       atgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGTACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATaa                                                                                                                                                         <  2:310389‑M2/107‑1 (MQ=255)                                                                                            gttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAattatt                                                                                                                                                    >  1:588547‑M2/140‑251 (MQ=255)                                                                                                 cgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTAtttttt                                                                                                                                                <  2:141077‑M2/116‑1 (MQ=255)                                                                                                    ccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGt                                                                                                                                            <  1:698012‑M2/120‑1 (MQ=255)                                                                                                          cggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATc                                                                                                                                      <  1:323308‑M2/126‑1 (MQ=255)                                                                                                                    cacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAgggg                                                                                                                            <  1:332384‑M2/136‑1 (MQ=255)                                                                                                                     acgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTTCTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAggggg                                                                                                                           <  1:436811‑M2/137‑1 (MQ=255)                                                                                                                        ggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTTAGTGAAATCCATACAGGGGGCaa                                                                                                                        <  2:231505‑M2/140‑1 (MQ=255)                                                                                                                           atttgcagtacttgccgaaggaagaatgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacaccggaatcctctctaaatacactaaccgaGTAGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCATCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGACTTATATAAGATAATTGTTTTTTTAGTGAAATCCATACAGGGGGAAAAt                                                                                                                      >  2:551052‑M2/109‑250 (MQ=255)                                                                                                                                cagtgcttgccgaaggaagattgagactattattgggtcctttggcgaacgatcaccaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGAGAATTGTAAAGATTCCTCAGTGGTCGCACCAGAATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCaaaa                                                                                                                 >  2:644564‑M2/104‑250 (MQ=255)                                                                                                                                  gtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAAGCaaaaaaa                                                                                                              <  2:431620‑M2/150‑1 (MQ=255)                                                                                                                                        gccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAAGTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCtat                                                                                                        <  2:476241‑M2/156‑1 (MQ=255)                                                                                                                                             aggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTc                                                                                                   <  1:647184‑M2/161‑1 (MQ=255)                                                                                                                                              ggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTc                                                                                                   >  1:361009‑M2/90‑250 (MQ=255)                                                                                                                                                   attgagtctattattgggtcctttggtgaacgatcaacaaagggacactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAGAGTCTATATTTCACTTTg                                                                                             <  2:614052‑M2/167‑1 (MQ=255)                                                                                                                                                        gtctattattgggtcctttggtgaacgctcaacaaagggccactagcaaacctgcttcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGACTTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTAATAAAAGGAAGTATTTGCCTGCATTATATACGCTAATTATTTTTTGAGTGAAATACATCCAGGGGACAAATCAAAAAAAGTCTATATTTCACTTTGAccgc                                                                                        >  2:473161‑M2/80‑251 (MQ=255)                                                                                                                                                            attattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgcc                                                                                     <  2:428164‑M2/175‑1 (MQ=255)                                                                                                                                                            attattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgcc                                                                                     <  2:495860‑M2/175‑1 (MQ=255)                                                                                                                                                                        tttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCCATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGTGAGAAATCCATACAGGGGGCAAATCAAACAAAGTCTATATTTCACTTTGCACGCGCCGCGAAAGTCACTg                                                                        >  2:666395‑M2/64‑251 (MQ=255)                                                                                                                                                                            gtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTT‑‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCa                                                                                                                    <  1:440714‑M2/143‑1 (MQ=255)                                                                                                                                                                            gtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTT‑‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCa                                                                                                                    >  2:440714‑M2/60‑202 (MQ=255)                                                                                                                                                                                     caacaaagggcctctagtccaccggattcctctctaaatcccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAAGTGTAAAGATGCCTCAGTGGTCGCACCAGCATCAATATGACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGGCGAGATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATaatgcgcagc                                                            <  2:585928‑M2/200‑4 (MQ=255)                                                                                                                                                                                                 actagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGTCGCGTTCATGTCCTCa                                               <  2:47525‑M2/213‑1 (MQ=255)                                                                                                                                                                                                   tagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCaa                                              >  1:68850‑M2/37‑250 (MQ=255)                                                                                                                                                                                                        cacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGGCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGc                                        <  1:340130‑M2/220‑1 (MQ=255)                                                                                                                                                                                                         cactgatctctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTAGCACCAGCATCAATATTACTAAAAGTAAGTAGTTGCCTGAATTATATAAGATAATTATTGTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCg                                       <  2:555797‑M2/221‑1 (MQ=255)                                                                                                                                                                                                             gattactctctacataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTCACAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCACGCGCCGAGACAGTCACTGATAATGCGCCACGTTCCTGTCATCAAAATGGCGTAAc                                   >  2:285272‑M2/27‑251 (MQ=255)                                                                                                                                                                                                                    ctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGGAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAt                            >  1:249116‑M2/20‑251 (MQ=255)                                                                                                                                                                                                                        aaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATg                           <  1:611665‑M2/233‑1 (MQ=255)                                                                                                                                                                                                                                   ccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGc             <  1:318442‑M2/247‑1 (MQ=255)                                                                                                                                                                                                                                   ccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTAGTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACAtttgtttg              <  1:86456‑M2/246‑1 (MQ=255)                                                                                                                                                                                                                                       gTTGCGCTCCTCTTA‑AAAAAAAGGACGTGAATTGTAAAGATTCCTCAGAGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTATTTGCTGGc         >  1:360093/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAattat                                                                                                                                                     >  1:679473/1‑110 (MQ=255)                                                                                                                                                                                                                                        ttGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGcc        >  1:256082/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTAGATAAGATAattat                                                                                                                                                     <  2:679473/110‑1 (MQ=255)                                                                                                                                                                                                                                          gcgcTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTTCTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCt       <  2:378931/250‑1 (MQ=255)                                                                                                                                                                                                                                               cctcTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGAtt  <  1:111318/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    AAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATT  >  NC_000913/3637181‑3637669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑