Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,307	G→A	L154L (CTC→CTT)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,307	0	G	A	100.0%	232.9 / NA	64	L154L (CTC→CTT)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/31);  total (33/31)

CCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGA  >  NC_000913/3815064‑3815545                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               ccGCTAATTGCGGCACAGTTGCATTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGaaa                                                                                                                                                                                                                                         <  2:345093/251‑1 (MQ=255) ccGCTAATTGCGGCACAGTTGAAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGACGCGCCCCCGTTCCTGACGATCAAGCGaa                                                                                                                                                                                                                                          >  2:423298/1‑250 (MQ=255)            ggCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGcc                                                                                                                                                                                                                               >  1:432589/1‑250 (MQ=255)                          attaggACGCCGGATGACTTTCCAGCCGGCGAGTTTCTTTAAACGCCAATCTCTTCGGGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATATGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCGGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAGGCGTCGCGcc                                                                                                                                                                                                                <  2:422798/246‑1 (MQ=255)                          aaTATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGCCGATCAAGCGAAATCAATAAGCCAGCAAGCGTCGCGcc                                                                                                                                                                                                                >  2:151919/1‑251 (MQ=255)                               gACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCAtt                                                                                                                                                                                                             >  2:285109/1‑249 (MQ=255)                                      ggtGACTTTTCATCCGGGGAGTTTCTTTAAANCCCAAACTCTTCGCGATCGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCTCCATTAGCCTGaat                                                                                                                                                                                                    <  2:212996/249‑1 (MQ=255)                                       aTGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGaat                                                                                                                                                                                                    >  1:346154/1‑250 (MQ=255)                                         gACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGaataat                                                                                                                                                                                                 <  1:285109/251‑1 (MQ=255)                                              ttCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTcc                                                                                                                                                                                             <  1:380916/250‑1 (MQ=255)                                                   ccGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCACGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGat                                                                                                                                                                                       >  2:51858/1‑250 (MQ=255)                                                     ggCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGattc                                                                                                                                                                                     >  1:26589/1‑248 (MQ=255)                                                       cGAGTTTCTTTAACCGCCAATCTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTGCCAGATAGGCAATCAGATCTTTCAGGGTGACGATAGAGATCACTTTGCAGTTTTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTcgc                                                                                                                                                                                   <  2:423434/251‑1 (MQ=255)                                                                ttAAACGCCAACCTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACTATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCAGCGATTCGCGAATCg                                                                                                                                                                             <  1:353073/248‑1 (MQ=255)                                                                      gCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCcgg                                                                                                                                                                    >  1:298604/1‑251 (MQ=255)                                                                         aaaCTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCcggcgg                                                                                                                                                                 >  2:322680/1‑251 (MQ=255)                                                                          aaCTCTTCGCGATAGGCCTTAACCGCCGCCCGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCcggcggg                                                                                                                                                                >  1:138404/1‑250 (MQ=255)                                                                                       aGGCTTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCt                                                                                                                                                   <  2:346322/251‑1 (MQ=255)                                                                                          ccTTAACCGCCGCCAGATGTTCCGACATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAAGGAGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGACGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGttctaaactcatcttac                                                                                                                                                >  1:196220/1‑235 (MQ=255)                                                                                              aaCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGc                                                                                                                                             >  1:46368/1‑250 (MQ=255)                                                                                               aCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTAAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGTCGATCAAGCGAAATCAATACGCCCGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACCTCTTCTACCGGCAt                                                                                                                                           >  1:86476/1‑251 (MQ=255)                                                                                                  gccgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAc                                                                                                                                        <  1:51858/251‑1 (MQ=255)                                                                                                    cgccAGATGTTCCGCCATGTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTGCCGGCGGTGAGCACATCATCTACCAGCATTAcgc                                                                                                                                      <  2:180019/251‑1 (MQ=255)                                                                                                        agctGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCTGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTcc                                                                                                                                  <  2:243854/248‑1 (MQ=255)                                                                                                              tCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTa                                                                                                                             <  2:72601/250‑1 (MQ=255)                                                                                                                 gCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCACCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCATTCCGCGTCCTTGTAAcgc                                                                                                                         >  2:291383/1‑251 (MQ=255)                                                                                                                   cATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTTCGCGTCCTTGTAACgcgc                                                                                                                       >  2:46016/1‑251 (MQ=255)                                                                                                                    aTTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCCCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGAACACATCATCTACCCGCATTACGCGTCCTTGTAACGCGCt                                                                                                                      >  1:296541/1‑251 (MQ=255)                                                                                                                        ccGGCTTCTCTTCCAGATAGGCAATCAGCTCTTTCAGGGTGATGATAGAGATCACTTCGCAGTTGTAATCACGCTCAACTTCCTGACTCGTCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGAACAAGCTAAATCAAGACGCCAGCAAGCGGCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAAcgctgtacca                                                                                                                  <  2:46884/251‑8 (MQ=255)                                                                                                                              tctcTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCATTTCGCGTCCTTGTAACGCGCTacccacc                                                                                                               >  2:282872/1‑248 (MQ=255)                                                                                                                              tctcTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTaccaacc                                                                                                               >  1:199728/1‑248 (MQ=255)                                                                                                                                      agataggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTgccgcc                                                                                                    <  1:111541/245‑1 (MQ=255)                                                                                                                                       gataggcaatcagagttttgaGGGGGATGATAGGGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTGCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCt                                                                                                   <  2:197223/231‑1 (MQ=255)                                                                                                                                        ataggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCtt                                                                                                  <  2:346154/247‑1 (MQ=255)                                                                                                                                            gCAATCAGATCTTGCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCAcc                                                                                              <  1:384498/251‑1 (MQ=255)                                                                                                                                                 cagatCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGCCCTTGTAACGCGCCACCAACCAGATTTCCGCCTTCCCCGTggg                                                                                         >  2:240550/5‑250 (MQ=255)                                                                                                                                                  agatCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCCACCAGATTGCCGCCTTCACCGTGGTc                                                                                        >  2:243618/4‑251 (MQ=255)                                                                                                                                                    atCTTTCAGGGTGATGATAGAGATCCCTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCt                                                                                                                                 <  2:241270/207‑1 (MQ=255)                                                                                                                                                    atCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCt                                                                                                                                 >  1:241270/2‑208 (MQ=255)                                                                                                                                                         tCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATTCCATCTCCTAACAGCATTACGTGTCCTTTTCACCCGCTACCACNCAGACTGCCACCCTCACCGTGGTCttttccc                                                                                 >  2:223825/1‑248 (MQ=255)                                                                                                                                                          cAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGccc                                                                                >  2:291027/1‑249 (MQ=255)                                                                                                                                                           aGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctc                                                                               <  1:38870/251‑4 (MQ=255)                                                                                                                                                             ggTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                                             >  1:146882/1‑251 (MQ=255)                                                                                                                                                             ggTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                                             <  1:329988/251‑1 (MQ=255)                                                                                                                                                               tgatgaTAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtct                                                                                                                                                                                               <  2:254776/135‑1 (MQ=255)                                                                                                                                                               tgatgaTAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtct                                                                                                                                                                                               >  1:254776/1‑135 (MQ=255)                                                                                                                                                                 atgatAGAGATCACTTTGCAGTTGTAATCACGCTCACCTTCCTGAATCGCCGACA‑TCTCGCCGCGCCCCAGTTCCTGACGATCAAACGAAATCAATACGCCAGCAAGCGTCGCGCCATCAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGAGGGGATCAAATCATCTACCAGCATTACGCGTCCCTTTAACGACCTCCCAACCAGATCGACCCCGTCACCGCGGACTTTTGcctatttccg                                                                         >  2:266748/1‑242 (MQ=255)                                                                                                                                                                 atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAC‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCCCATCATCTCCAAGCATTAAGCGTCCTTGTAACGCGCTACCCACCAGATTGCCGCCTTCACCCGGGTCTTTTGCCTCTTTGCg                                                                         >  2:88163/1‑251 (MQ=255)                                                                                                                                                                     tAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGACA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGACTCGCCGTTCCGGCGGTGATTACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTCCCCACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGttc                                                                     >  2:88290/1‑250 (MQ=255)                                                                                                                                                                          aTCACTTTGCATGTGTAATCACGCTCAACGTCCTTAATCGCCGAAA‑TCTCGCCTCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCTCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCCTTCCGGCGGTGATCACATCATCTACCTGCAGTACGCGTCCTTGTACCGCGCTGCCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCTTTTCGGTTGAAGCa                                                                <  2:242623/251‑1 (MQ=255)                                                                                                                                                                             aCTTTGCAGTTGTAATCACGCTCATCTTCCTGAATCGCCGAAAGTC‑CGCCGCGCCCCGGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAATCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGGATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTGTTGCCTCGTTGCGGTTAAAGCAGTa                                                             <  2:84347/251‑1 (MQ=255)                                                                                                                                                                                 tGCAGTTGTAATCACGCTCAACTCCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCTGTTAAAGCAGTACgg                                                          <  2:265619/250‑1 (MQ=255)                                                                                                                                                                                         tAATCACGCTCAACTTCATGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTTCCGCCTTCACCGTGGTCTTTTTCCCCTTTGCGGTTAAAGCAGTACGGcagggcc                                                   >  2:367969/1‑246 (MQ=255)                                                                                                                                                                                           atcccGCTTAACTTCCTTAATCGCCGAAA‑TCTCGCCGCGCCCCCGGTCCTGACTATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGTCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCTGTTAAAGCAGTACGGcaggtcaaggt                                               <  1:35227/247‑1 (MQ=255)                                                                                                                                                                                                ccTCAACTTCCTGAACCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGa                                          <  1:423237/250‑1 (MQ=255)                                                                                                                                                                                                        tCCTGAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCTcccc                                  >  1:422325/1‑249 (MQ=255)                                                                                                                                                                                                            gAATCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAgc                              <  2:146882/251‑2 (MQ=255)                                                                                                                                                                                                             aaTCGCCGAAA‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGAGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGcagggccagggtatggtggcccgcccccg                             >  2:375105/1‑232 (MQ=255)                                                                                                                                                                                                              aTCGCCGAAA‑TCTCCCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAgcgc                            <  2:191190/251‑4 (MQ=255)                                                                                                                                                                                                                       a‑TCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTGCTGGTGCTCCggccgcgccaccggggg                   >  1:382223/1‑235 (MQ=255)                                                                                                                                                                                                                          ctcGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCAAGATGGTCCGCCAGCGccccggcggggg                 >  2:327145/1‑241 (MQ=255)                                                                                                                                                                                                                                 cgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAgtggtcgc          <  1:9585/251‑4 (MQ=255)                                                                                                                                                                                                                                    ccccgTTCCGGACGATCAAGCGAAACCAATCCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCAAATCGCCGTTCCGGCGGTGAGCTCATCATCTCCCAGCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTGTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAgtggtcgcgac       <  2:37360/248‑7 (MQ=255)                                                                                                                                                                                                                                          tCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGa  <  2:43009/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               CCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAA‑TCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGA  >  NC_000913/3815064‑3815545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑