| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,697,170 | C→T | A238V (GCG→GTG) | bcsE → | c‑di‑GMP‑binding protein BcsE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,697,170 | 0 | C | T | 91.7% | 133.1 / ‑5.1 | 49 | A238V (GCG→GTG) | bcsE | c‑di‑GMP‑binding protein BcsE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/18); minor base G (4/0); total (31/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
AACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGCGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCTGCGGTGCA > NC_000913/3696924‑3697411 | aaCAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGtgccg > 1:223432/1‑251 (MQ=255) aaTTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGANGAATGTTGCTGTACTGGAAGGTGtgccgccg > 2:213914/1‑251 (MQ=255) cATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCg > 1:17630/1‑251 (MQ=255) aTTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAGACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGGGCCGCCGCTATCgg > 2:278242/1‑251 (MQ=255) ttgttgCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGGGTGCCGCCGCTATCgg > 1:38844/1‑250 (MQ=255) tgttgCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGCCCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGCAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGGGCCGCCGCTATCGGa > 2:257758/1‑250 (MQ=255) tgttgCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGCAAAAGGGGTCAGCGC‑CCGTCAGCAGATTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAACCGCATTCTGAGTAATGTTGCTGTACTGGAATGTGTGCCGCCGCTATCGGa > 1:412981/1‑250 (MQ=255) tgttgCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGGGTGCCGCCGCTATCGGa > 2:104900/1‑250 (MQ=255) ttGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTgg > 1:98274/1‑251 (MQ=255) aCCGTTCCCTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAGACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTc < 1:278242/251‑1 (MQ=255) ccAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTc < 1:185159/251‑1 (MQ=255) gtgcagctaaaaaGGGGTCA‑CGCTCCGTCATCAACTTATCGTTCTGCAACAAAATGTTATCTGG‑ACAGTAATTCAAAGCGAAGAGCCAGAGATCCAACCCCGCAGCGACGAAAAACGCGTTCTTATTAATGTTGCTGTACTGGAAGTTGTGCCGCCGCTATCGTAACACGGGCAACTGTTCAACAATAACGAAGTCCTTTTCAATGAAGCCCGTACCGCTCAGGCGGCGACTGTGGTCTTGTCTTTACatc < 2:310892/243‑3 (MQ=255) aCGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTTGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTTAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAAt < 1:130512/251‑1 (MQ=255) aCGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAAt > 1:235195/1‑251 (MQ=255) ggggTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAAGGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAAt < 2:410554/251‑1 (MQ=255) cAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGc > 2:141365/1‑251 (MQ=255) aGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGcc > 1:325515/1‑251 (MQ=255) gTCAGCAGCTTAGCGATCAGCAACAAAATGGAATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGAGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGACGCCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCCCAAATCGAGCCCCTGGcc > 2:54700/1‑251 (MQ=255) cagcagCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGCGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCg > 1:224848/1‑251 (MQ=255) gcTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGCCGAAAAACGCATTCTGAGCAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGATCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCANAATGCGCAAATCGAGCCACTGGCCCgcagc > 2:216042/1‑250 (MQ=255) cAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGGGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATAccc < 2:349158/250‑1 (MQ=255) acaaAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCCTTCATACCCTGCgcc > 2:13152/1‑249 (MQ=255) aaaaaaTGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTc < 2:98274/249‑1 (MQ=255) aaaaaaTGGTA‑CTGGGACATTAGTTCAAAGGGAAGAGGAGGAGACCCACCCACGCAGCGTCGAAAACCTCATTCTGATTAATGTTGCTGTACTGGATGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCTAGCCACTGGCCCGCAGCATTCATACCCTGCGTc < 2:311530/249‑1 (MQ=255) caaAATGGAATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGGGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCg > 1:195296/1‑251 (MQ=255) aaagggTATCTGG‑ACATTAGTTAAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCGGTACTGGAAGGTGTGCCGCCGTTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGcc < 1:54700/247‑1 (MQ=255) aaaTGGTATCTGG‑ACATTAGTTCAAAGCGAANGGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGcc < 2:235195/251‑1 (MQ=255) aaaTGGTATCTGG‑ACAGTAGTTCAAAGCGAAGAGGCGGAGATCCACCCACGCAGGGACGAAAAACGCATTCTGATTAATGTTGCTGAACTGGAAGGGGTGCCGCCGCTATCGGAACACTGGCAACTGTCCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGcc < 2:130661/251‑1 (MQ=255) ggTAACTGG‑ACATTAGTTCAAAGCGAATAGGCGGATATCCAACCACGCAGCGACTAAAAACGCTTTCGGATTAATGTTGCTGTACGGGAAGGTGTGCCGCCGCTATCGGACCACTGGCAACTTTTCAACAATAACGAAGTCCTGTTCAATGAGGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTGCTTTACAGCAAATTGCGCAAATCGAGCCACTGGCCCGCAGCATTCATTCCCTGCGTCGCCAgc < 2:257636/250‑1 (MQ=255) gTTCAAAGCGAAGAGGCGGAGATCCAAACACGCAGCGACGAAAAAAGCATTCTGAGTAATGTTGCTGTACAGGAAGGTGAGCCGCCGCTATCGGAACACTGGCAACTGTTCCACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTAAAGCAAAATGGGCAAATCGAACCACTGGCTCGCAGCATTCATACCCTGCGTCTCCAGCGCGGAAGAGCGATGa > 2:48246/1‑250 (MQ=255) cAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAAt > 1:28222/1‑251 (MQ=255) gCGAAGAGGCGGAGATCCAACCCCGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTc < 1:13152/251‑1 (MQ=255) cGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCg > 1:409352/1‑251 (MQ=255) ggCGGAGACCCAACCACGCAGCGACGAAAAACGCATTCGGAGTAATGTGGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCggg < 2:17630/251‑1 (MQ=255) ggAGATCCAACAACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCCGTTCAATGAAGCACGTAACGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGAGGGAGTGCGATGAAAATCCTCTTGCGGGaa > 2:39368/1‑250 (MQ=255) gaTCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAAt > 1:459827/1‑250 (MQ=255) cGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCt > 1:453421/1‑251 (MQ=255) aGCGACGAAAAAAGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAACTACCGCTAGCCTgcg > 2:31355/1‑251 (MQ=255) gacgaAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTgcgcgc > 1:438981/1‑251 (MQ=255) acgaAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGcc > 1:128032/1‑251 (MQ=255) acgaAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGcc > 2:158538/1‑251 (MQ=255) gAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGttt < 1:141365/249‑1 (MQ=255) gAGTAATGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGt > 1:111867/1‑251 (MQ=255) aaTGTTGCTGTACGGGACGTTGTGCCGCCGCNATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTAtt < 2:223432/251‑1 (MQ=255) aTGTTGCTGTACTGAAAGTTGGGCAGTCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGCCGACGACGGAGGTATTTTCTTTACAGCCAAATGCGCAATTCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGTTGAAAACCCTCTTGCGGGGAACTACCTCTGTCCCGCGCCCCGCCGCTGAACATTTGTTAtgc > 2:275564/1‑249 (MQ=255) tGTTGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGCAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAACTACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTgg > 1:227175/1‑251 (MQ=255) tGCTGTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGCGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGCAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCt > 2:332488/1‑251 (MQ=255) cTGTACTGGAAGGAGTGCCGCCGCTATCGGAACACTGGAAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTCGTGCGATGAAAATCCTCGTGCGGGCAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCTGc > 1:131328/1‑251 (MQ=255) gTACTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCTGCgg < 1:213914/251‑1 (MQ=255) aCTGGAAGGTGTGCCGCCGCTATCGGAACACTGGCAACGGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCTGCGGt < 1:85044/250‑1 (MQ=255) ggAAGGTGTGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCTGCGGTGCa > 1:89019/1‑250 (MQ=255) | AACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGC‑CCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGG‑ACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAGTAATGTTGCTGTACTGGAAGGTGCGCCGCCGCTATCGGAACACTGGCAACTGTTCAACAATAACGAAGTCCTGTTCAATGAAGCCCGTACCGCTCAGGCGGCGACGGTGGTCTTTTCTTTACAGCAAAATGCGCAAATCGAGCCACTGGCCCGCAGCATTCATACCCTGCGTCGCCAGCGCGGTAGTGCGATGAAAATCCTCGTGCGGGAAAATACCGCTAGCCTGCGCGCCACCGATGAACGTTTGTTATTGGCCTGCGGTGCA > NC_000913/3696924‑3697411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |