Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,436	G→A	intergenic (‑26/‑206)	yhiN ← / → pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,436	0	G	A	100.0%	177.5 / NA	47	intergenic (‑26/‑206)	yhiN/pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/31);  total (16/31)

GCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGC  >  NC_000913/3637193‑3637681                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAAtt                                                                                                                                                                                                                                               <  1:364012‑M2/33‑1 (MQ=255)   acatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGt                                                                                                                                                                                                                                             <  1:143779‑M2/35‑1 (MQ=255)      tcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTaa                                                                                                                                                                                                                                           >  1:124223‑M2/214‑250 (MQ=255)              gatatctgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTc                                                                                                                                                                                                                                  <  2:411469‑M2/46‑1 (MQ=255)              gacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTc                                                                                                                                                                                                                                  <  1:188589‑M2/46‑1 (MQ=255)                  actgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTg                                                                                                                                                                                                                              <  1:11067‑M2/50‑1 (MQ=255)                   ctgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgccgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGCAAAGATTCCTcagat                                                                                                                                                                                                                              >  1:411469‑M2/201‑248 (MQ=255)                     gtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTc                                                                                                                                                                                                                           <  1:11127‑M2/53‑1 (MQ=255)                          gaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattatggggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCAcc                                                                                                                                                                                                                      <  2:35528‑M2/58‑1 (MQ=255)                            aatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAg                                                                                                                                                                                                                    <  2:70809‑M2/60‑1 (MQ=255)                             atcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgcagatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGc                                                                                                                                                                                                                   >  1:394537‑M2/191‑251 (MQ=255)                                  cgtgttgaaaggtgacatgggtggcgtgcagcattttgccgatggtgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCaa                                                                                                                                                                                                              <  2:133893‑M2/66‑1 (MQ=255)                                   gtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAat                                                                                                                                                                                                             >  2:340573‑M2/185‑251 (MQ=255)                                   gtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagagtgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGAGGTCGCACCAGCATCAat                                                                                                                                                                                                             <  2:383572‑M2/67‑1 (MQ=255)                                         aaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACt                                                                                                                                                                                                       <  1:77797‑M2/73‑1 (MQ=255)                                           agctgacatgggtgacgtgcagcattttgccgatggcgttatcccccagcgcggcgtgcgtcacgggcatttgcagtgcttgccgaaggaggattgagtctattattgtatcctttggtgaacgaacaacaaatcgccactagcacacctgattcctctctaaataccctatcccaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCTCACCTGCATCACTAGTACTaa                                                                                                                                                                                                     <  2:343626‑M2/75‑1 (MQ=255)                                                          cgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCGCCAGCATCAATATTACTAAAAGGAAGTATTTGcc                                                                                                                                                                                      <  2:78761‑M2/90‑1 (MQ=255)                                                             gcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGa                                                                                                                                                                                   <  1:250552‑M2/93‑1 (MQ=255)                                                                  attttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagagtgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaatacccgatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATAAATATTACTAAAAGGAAGTATTTGCCTGAATtat                                                                                                                                                                              <  1:456114‑M2/98‑1 (MQ=255)                                                                      tgccgatgtcgttatctcccagcgcggcgtgcggcacgggcatgtgcagtgcgtgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggcctctagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATGCCTCAGTGGTCGCACCATCATCAATATTACTAAAAGGAAGTATTTGCCTGAattataga                                                                                                                                                                           <  2:336316‑M2/101‑3 (MQ=255)                                                                                   atcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTAtttt                                                                                                                                                              <  1:11858‑M2/114‑1 (MQ=255)                                                                                                gcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCa                                                                                                                                                <  1:297922‑M2/128‑1 (MQ=255)                                                                                                  gggcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATa                                                                                                                                              <  2:300292‑M2/130‑1 (MQ=255)                                                                                                     cggcgcgggcatttgcagtgcttgtcgaatgaagatagcgtttattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgactcctctctaaataccctatacgaGCTGCGCTCCTCTTA‑AAAAAGCGGACGTGAATTGTAAAGATTCCTCAGTGGTAGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAACATAATTATTTTTTGAGTGAAATCCATACAg                                                                                                                                           <  1:242797‑M2/133‑1 (MQ=255)                                                                                                         acgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGt                                                                                                                                                                                                                               >  1:405278‑M2/115‑163 (MQ=255)                                                                                                         acgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGt                                                                                                                                                                                                                               <  2:405278‑M2/49‑1 (MQ=255)                                                                                                           gggcatttgcagtgcttgccgaaggaagattgagtctattagtgggtcctttggtgaacgatcaacaaagggcccctagcacccctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCa                                                                                                                                     <  2:109562‑M2/139‑1 (MQ=255)                                                                                                               atttgcattgcttgccgaaggaagattgagtctattagtgggtccgttggtgaacgatcaacaaagggcctctagcacacctgattcctctctaaacaccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATGGTAAAGATTCCGCAGTGGTCGCACCAGTATAAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTGGAGTGAAATCCATACAGGGGGCAAATc                                                                                                                                 <  1:420729‑M2/143‑1 (MQ=255)                                                                                                                            gccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCtat                                                                                                                    <  2:118023‑M2/156‑1 (MQ=255)                                                                                                                                    aagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACt                                                                                                            >  1:152486‑M2/88‑251 (MQ=255)                                                                                                                                      gattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCGTA‑AAAAAACGGACGTGAATTGTAAAGATTCCGCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATAGTtcacgtt                                                                                                          <  2:138260‑M2/166‑4 (MQ=255)                                                                                                                                           agtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGCAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccg                                                                                                     >  1:402497‑M2/81‑251 (MQ=255)                                                                                                                                              ctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATCTCAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgc                                                                                                  >  1:207139‑M2/78‑251 (MQ=255)                                                                                                                                                  tattgagtcctttggggaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGAAAATCAAAAAAAGTCTATATTTCACTTTGCccgctccgcg                                                                                              >  2:341052‑M2/74‑251 (MQ=255)                                                                                                                                                   attgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGa                                                                                             <  1:419468‑M2/179‑1 (MQ=255)                                                                                                                                                          cctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCAc                                                                                      >  2:239868‑M2/66‑251 (MQ=255)                                                                                                                                                                    acgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATgcg                                                                            <  1:239868‑M2/196‑1 (MQ=255)                                                                                                                                                                                gggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGt                                                                <  1:93569‑M2/208‑1 (MQ=255)                                                                                                                                                                                gggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGt                                                                >  1:175877‑M2/44‑251 (MQ=255)                                                                                                                                                                                           acacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTAttt                                                                                                                                                                                         <  1:461394‑M2/87‑1 (MQ=255)                                                                                                                                                                                           acacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTAttt                                                                                                                                                                                         >  2:461394‑M2/33‑119 (MQ=255)                                                                                                                                                                                           acacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATgg                                                     >  2:458496‑M2/33‑251 (MQ=255)                                                                                                                                                                                                                accctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACAtt                                <  1:458496‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                     atccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACAtttgttt                           >  2:108624‑M2/7‑251 (MQ=255)                                                                                                                                                                                                                                            aaaaaCGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCg     >  1:37579/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaaCGGACGTGAATTGTAAAGATGCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGGCTGGATTTGCATACCggg   <  2:394537/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaCGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGc  >  1:28164/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGC  >  NC_000913/3637193‑3637681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑