Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,173,431	T→A	I97N (ATC→AAC)	ygiN →	putative quinol monooxygenase YgiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,173,431	0	T	A	100.0%	208.4 / NA	57	I97N (ATC→AAC)	ygiN	putative quinol monooxygenase YgiN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (24/33);  total (24/33)

ATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTTCGCGCA  >  NC_000913/3173185‑3173676                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ataaacggcaacgcacggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGt                                                                                                                                                                                                                                                   <  2:106971‑M2/10‑1 (MQ=255)             gcacggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGcc                                                                                                                                                                                                                                        >  2:30450‑M2/230‑250 (MQ=255)                       tgatcacgtacatggagtagtttaagaaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAAt                                                                                                                                                                                                                             >  1:27670‑M2/220‑251 (MQ=255)                        gatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttatgcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATc                                                                                                                                                                                                                            <  2:344729‑M2/33‑1 (MQ=255)                           cacgttcatggagtagtttaagtgggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCt                                                                                                                                                                                                                          <  2:331959‑M2/35‑1 (MQ=255)                           cacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTg                                                                                                                                                                                                                         <  2:46206‑M2/36‑1 (MQ=255)                               tacatgtagtagtttaagtaggcgttttccttaaattcatgtagcgcacggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagctttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGcc                                                                                                                                                                                                                       <  1:225083‑M2/38‑1 (MQ=255)                                 catggagtagtttaagtaggcgttttccgtaaattcatggagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatagtgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGt                                                                                                                                                                                                                   <  1:235012‑M2/42‑1 (MQ=255)                                  atggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGtt                                                                                                                                                                                                                  >  1:141905‑M2/209‑251 (MQ=255)                                    ggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGttt                                                                                                                                                                                                                 <  1:108049‑M2/44‑1 (MQ=255)                                         agtttaagtaggcgttttccttaaattcaggtagcgcaaggcccgctgccatatcgctcattaattctgattcctcaacttatgcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcggcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTGTTTTGCCCgg                                                                                                                                                                                                           <  2:287670‑M2/50‑1 (MQ=255)                                               attaggcgttttccgaaaattcatttagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATcc                                                                                                                                                                                                     <  2:39503‑M2/56‑1 (MQ=255)                                                     cgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCg                                                                                                                                                                                               <  2:160953‑M2/62‑1 (MQ=255)                                                      gtttgccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattcttattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCgg                                                                                                                                                                                              <  1:342774‑M2/63‑1 (MQ=255)                                                          tccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGCTTTAATCCTGCCTTGTTTGCACGGCCATCCTGACCGGGCa                                                                                                                                                                                           >  2:296893‑M2/185‑250 (MQ=255)                                                           ccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAAt                                                                                                                                                                                         <  1:135151‑M2/68‑1 (MQ=255)                                                           ccgtaaattcatggagcgcaaggcgctctgccatatcgctcattaattctgattccgcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGCCCATCCTGACCGGGCAAt                                                                                                                                                                                         <  2:389943‑M2/68‑1 (MQ=255)                                                            cgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAgg                                                                                                                                                                                                                                     >  1:175883‑M2/183‑206 (MQ=255)                                                            cgtaaagtcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAgg                                                                                                                                                                                                                                     <  2:175883‑M2/24‑1 (MQ=255)                                                             gtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGt                                                                                                                                                                                       <  1:178066‑M2/70‑1 (MQ=255)                                                             gtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGt                                                                                                                                                                                       >  1:215827‑M2/182‑251 (MQ=255)                                                               aaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGtt                                                                                                                                                                                      <  1:49982‑M2/71‑1 (MQ=255)                                                                   tcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCttt                                                                                                                                                                                  <  2:433173‑M2/75‑1 (MQ=255)                                                                         acctcaaggcgctctgccatatctctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatatggccggagatacgacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTaa                                                                                                                                                                           <  2:104718‑M2/82‑1 (MQ=255)                                                                                      ctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAAtctc                                                                                                                                                                <  2:271986‑M2/93‑1 (MQ=255)                                                                                         ccatatcgctcattaattctgattcctcaacgtattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCa                                                                                                                                                           <  2:42940‑M2/98‑1 (MQ=255)                                                                                             atcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTc                                                                                                                                                       >  2:365684‑M2/150‑251 (MQ=255)                                                                                              tcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTCCCTTTAAACCTCAATCTCCGCCATGTCg                                                                                                                                                      >  1:346637‑M2/149‑251 (MQ=255)                                                                                                    attaattctgattcctcatcttattagccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCAGGTCGCCTTtc                                                                                                                                                >  2:391692‑M2/143‑251 (MQ=255)                                                                                                     ttaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTtct                                                                                                                                               >  2:175052‑M2/142‑251 (MQ=255)                                                                                                             gattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAAc                                                                                                                                       >  2:126925‑M2/134‑251 (MQ=255)                                                                                                                          attcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGAt                                                                                                                          <  2:141905‑M2/131‑1 (MQ=255)                                                                                                                              gccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagataaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTc                                                                                                                      >  1:416764‑M2/117‑251 (MQ=255)                                                                                                                                         aaggggcaatattgccggagatactacagtatcggccgcgtcgagtcacaggatatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcagcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCGTCCTGACCGGGTAATGTTCTTGCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGATCGTttct                                                                                                           <  2:320745‑M2/146‑1 (MQ=255)                                                                                                                                             gtcaatattgacggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgggctcagacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCAATCCTGACCGGGCAATGTTCTTTCCTTTAACCCTCAATCTCCGCCATGTCGCTTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGcgtttcttagc                                                                                                       <  1:353528‑M2/150‑4 (MQ=255)                                                                                                                                              gcaatattgccggagatactacattatctgccgcctcgagtcacaggagctcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGcc                                                                                                      >  2:128703‑M2/101‑251 (MQ=255)                                                                                                                                                           agatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCca                                                                                         <  1:99471‑M2/164‑1 (MQ=255)                                                                                                                                                                   cattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTg                                                                                                                                                                                                   <  2:98516‑M2/58‑1 (MQ=255)                                                                                                                                                                   cattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTg                                                                                                                                                                                                   >  1:98516‑M2/80‑137 (MQ=255)                                                                                                                                                                      tatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGtcc                                                                              >  1:162680‑M2/77‑250 (MQ=255)                                                                                                                                                                             cgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCt                                                                       <  1:204134‑M2/182‑1 (MQ=255)                                                                                                                                                                               cgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGAAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGTTCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                                      >  1:27678‑M2/68‑250 (MQ=255)                                                                                                                                                                                    agtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTc                                                                <  1:154369‑M2/189‑1 (MQ=255)                                                                                                                                                                                     gtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCt                                                               >  1:8746‑M2/62‑251 (MQ=255)                                                                                                                                                                                       cacaggagatcaacgtgaatagcgccgccgnacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCtt                                                              <  2:215827‑M2/191‑1 (MQ=255)                                                                                                                                                                                          aggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCAGCATCGCGTCAGTACGCTGATCGTCTtcatc                                                          <  1:365684‑M2/195‑1 (MQ=255)                                                                                                                                                                                            gagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTcatcat                                                        <  1:195723‑M2/197‑1 (MQ=255)                                                                                                                                                                                                        aatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACt                                            <  1:30450‑M2/209‑1 (MQ=255)                                                                                                                                                                                                                    ggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAACCCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTATTCATCATCGATAGTCAACTGCACCAGACGGc                                >  1:204930‑M2/31‑251 (MQ=255)                                                                                                                                                                                                                      ccgggctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAATCCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGa                              <  2:155524‑M2/223‑1 (MQ=255)                                                                                                                                                                                                                       cgtgctccgacggcgataaaggcatcaAAATAACCTTATTCTGCAGCCAGGGTTTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAATCCTCAATCTCCGCCATGTCGCCTTTCTCTTGCATCCAGTTGCGGCTATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATATTCAACTGCACCAGACGGCGAg                             <  1:37192‑M2/224‑1 (MQ=255)                                                                                                                                                                                                                          gctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCNTCGATAGTCAACTGCACCAGACGGCGAgtgt                          >  2:205656‑M2/25‑251 (MQ=255)                                                                                                                                                                                                                              cgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCgg                      >  1:344987‑M2/21‑251 (MQ=255)                                                                                                                                                                                                                                   gcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCa                  >  2:444656‑M2/16‑250 (MQ=255)                                                                                                                                                                                                                                      ataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCg              >  1:318390‑M2/13‑251 (MQ=255)                                                                                                                                                                                                                                                  gAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTTCgcgc   >  1:80391/1‑250 (MQ=255)                                                                                                                                                                                                                                                   aaTAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTTCGCGCa  >  2:68988/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTTCGCGCA  >  NC_000913/3173185‑3173676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑