Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,177	A→G	intergenic (‑116/+257)	rfbB ← / ← galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,177	0	A	G	100.0%	205.9 / NA	56	intergenic (‑116/+257)	rfbB/galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/27);  total (29/27)

GTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACA  >  NC_000913/2112935‑2113417                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ggacattacatactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGcc                                                                                                                                                                                                                                          >  1:293430‑M2/133‑251 (MQ=255)        acatactcctgtcaggtgtgatccccgcgttgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTatgat                                                                                                                                                                                                                                    <  1:90201‑M2/125‑1 (MQ=255)               cctgtcaggtgtgatcaccgcgctgaaggctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACg                                                                                                                                                                                                                              <  1:265742‑M2/131‑1 (MQ=255)                     aggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTAc                                                                                                                                                                                                                      <  1:146801‑M2/139‑1 (MQ=255)                     aggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTAc                                                                                                                                                                                                                      >  1:326153‑M2/113‑251 (MQ=255)                           gatcaccgcgcggaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTc                                                                                                                                                                                                                <  2:339387‑M2/145‑1 (MQ=255)                            atcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTcg                                                                                                                                                                                                               >  1:314636‑M2/106‑251 (MQ=255)                                   cgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTaaa                                                                                                                                                                                                        <  1:388733‑M2/153‑1 (MQ=255)                                       gaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCg                                                                                                                                                                                                    >  1:164352‑M2/95‑251 (MQ=255)                                              cgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAAtt                                                                                                                                                                                             >  1:111647‑M2/88‑251 (MQ=255)                                               gcggcctggaatgttcgcaaataagtatacaaattacttattaaagtataaatagcttatccatgcttatattcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTc                                                                                                                                                                                            <  1:55034‑M2/165‑1 (MQ=255)                                               gcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAAt                                                                                                                                                                                              <  2:192673‑M2/163‑1 (MQ=255)                                               gcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAAt                                                                                                                                                                                              >  1:192673‑M2/87‑249 (MQ=255)                                                   cctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAc                                                                                                                                                                                        <  1:26277‑M2/169‑1 (MQ=255)                                                    ctggaatgttcgcaaataaatatacaaagtaattattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCAATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGAGAGCATGTCTCTGATGTGTCGAGCGTTACCCACTCGCGctaaacccgaaaacttacaccg                                                                                                                                                                                       >  2:426313‑M2/82‑242 (MQ=255)                                                     tggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGc                                                                                                                                                                                      >  2:53055‑M2/81‑251 (MQ=255)                                                        aatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTa                                                                                                                                                                                    >  1:456577‑M2/78‑250 (MQ=255)                                                            ttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatagtcttacggctttatTTATTCAATATTACTTCAGAGCATGCTGTGCAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCATGGCTTTACAGCTACCAGCGCACTGCGTGCATGCCTATGATGGGAAGAGCGTTACCCACTCGCGATATACCCGAAAAATTCACACGCTAATTGt                                                                                                                                                                               >  2:169988‑M2/74‑251 (MQ=255)                                                                     acgtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCAATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAAt                                                                                                                                                                      >  2:415447‑M2/65‑251 (MQ=255)                                                                         atacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggatttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGc                                                                                                                                                                  >  2:282746‑M2/61‑251 (MQ=255)                                                                           acaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGAGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGctc                                                                                                                                                                >  1:69752‑M2/59‑251 (MQ=255)                                                                            caaagttcttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTTTGAAATAAGCGGCTCTCATTTTGATTAATAGAGTTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGctct                                                                                                                                                               <  2:408740‑M2/194‑1 (MQ=255)                                                                             aaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAc                                                                                                                                                                                        >  1:363410‑M2/57‑225 (MQ=255)                                                                             aaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTGCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAc                                                                                                                                                                                        <  2:363410‑M2/169‑1 (MQ=255)                                                                                        taaagtataaatagcttatccaagcttatatgctgacggctttatTTATTCCATATTACTACATAGCATGCTGTGAAATACGCGGCTCTCAGTTTGATTAATAGAGGTAGGAATGCACGCTTCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGa                                                                                                                                                    <  2:118128‑M2/205‑1 (MQ=255)                                                                                           agtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTCATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAAt                                                                                                                                                >  1:439581‑M2/43‑251 (MQ=255)                                                                                           agtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAAt                                                                                                                                                <  1:136023‑M2/209‑1 (MQ=255)                                                                                               taaatagcttacccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCt                                                                                                                                             <  1:187081‑M2/212‑1 (MQ=255)                                                                                                     gcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAAc                                                                                                                                      <  1:220807‑M2/219‑1 (MQ=255)                                                                                                                   atatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAAccc                                                                                                                        <  1:34832‑M2/233‑1 (MQ=255)                                                                                                                        cttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTg                                                                                                                   >  1:408672‑M2/14‑251 (MQ=255)                                                                                                                                       aTTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAACCCCTACTGCTAACTCGTTGTTAt                                                                                                    >  2:413380/1‑251 (MQ=255)                                                                                                                                          ccATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCt                                                                                                 <  1:331490/251‑1 (MQ=255)                                                                                                                                           cATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTg                                                                                                >  2:412978/1‑251 (MQ=255)                                                                                                                                                 aCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGtt                                                                                          >  1:359002/1‑251 (MQ=255)                                                                                                                                                        agCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTtatata                                                                                    >  1:371743/1‑250 (MQ=255)                                                                                                                                                                gtgAAATAAGCGGCTCTCAGCTTGATTAATAGAGGTATTATTGCACGCTACCGCCCCTAGCTTTTCAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGATCGTTACTCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTTCCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAACCCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaacaac                                                                             <  1:276151/249‑1 (MQ=255)                                                                                                                                                                   aaaTAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTCCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAg                                                                        <  2:326153/251‑1 (MQ=255)                                                                                                                                                                     aTAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGa                                                                      <  2:185725/251‑1 (MQ=255)                                                                                                                                                                       aaGCGGGTCTCAGTTTGATTAATAGAGGTATTAAGGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAg                                                                    <  2:256247/251‑1 (MQ=255)                                                                                                                                                                         gCGGCTCTAAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTGATATAAAACAACGGCAGGAAGAt                                                                  <  1:412978/251‑1 (MQ=255)                                                                                                                                                                          cGGCTCTCAGTTTGATTAATAGAGGTATTACTGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTCTTCTGATGGTTTATATAAAACAACGGCAGGAAGAtt                                                                 >  2:311333/1‑251 (MQ=255)                                                                                                                                                                           ggCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGAttt                                                                >  1:236314/1‑250 (MQ=255)                                                                                                                                                                           ggCTCTCAGTTGGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTc                                                                <  1:282746/251‑1 (MQ=255)                                                                                                                                                                                 cAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAATCAACGGCAGGAAGATTCGcaac                                                           >  1:228262/1‑250 (MQ=255)                                                                                                                                                                                       gATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTAc                                                    >  1:177709/1‑251 (MQ=255)                                                                                                                                                                                             aaaagGTATTAATGCCCGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgc                                              <  2:177709/248‑1 (MQ=255)                                                                                                                                                                                              agtggtaataaggactgctcccGCCCCTTGCTTTGCAGCTACCAGAGCACTGCGGGCATGCCTATGATGTGACTAGCGTTACCCACTCCCGCTAATCCCGAAAAATTCATACGCTAATTGTCTTACCAATCCGCTCTGGAAACAACGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTTTTATTATGATGGTTTATATAAATCAACGGCAGGCAGGTTCGCAACAAATTACTTTtgcc                                             <  2:11013/231‑2 (MQ=255)                                                                                                                                                                                                  gTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCg                                          >  1:345620/1‑250 (MQ=255)                                                                                                                                                                                                    aTTAATGCACGCTACCGCCCCTGGCTTTACAGCTCACAGAGCAATGCGTGCATGCCTATGATGGGACGCGCGTTACCCACTCGCGCTAAACCCGAAAAATTCACACGCTAAGTGTCTTACCAATCCGCTCTGAAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACAGCAGGAAGGTTAGCAACAAAATACTTTTGCTGCgga                                        >  2:411970/1‑248 (MQ=255)                                                                                                                                                                                                    aTTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAAt                                       >  1:375347/1‑251 (MQ=255)                                                                                                                                                                                                             cGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGc                              <  1:182348/251‑1 (MQ=255)                                                                                                                                                                                                                tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGt                           >  1:126547/1‑251 (MQ=255)                                                                                                                                                                                                                    gCCCCTGACTTTACAGCTACCAGAGCACAGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAACTCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGACGTtatt                       >  1:367595/1‑250 (MQ=255)                                                                                                                                                                                                                                      aCCAGAGCCCTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCGGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCg       <  2:370303/249‑1 (MQ=255)                                                                                                                                                                                                                                         agagCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACa  <  1:186123/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACA  >  NC_000913/2112935‑2113417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑