Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,924	C→T	S73L (TCA→TTA)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,924	0	C	T	100.0%	136.4 / NA	35	S73L (TCA→TTA)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/14);  total (21/14)

CAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG  >  NC_000913/4437686‑4438165                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  cAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTATATTGGTGTGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGAATTTAAGAAAAAAGATTGTATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTCATAAAAGGGGTTATCAGGTGTTTTCATTAAAAAATGAAAc                                                                                                                                                                                                                                       <  2:377018/251‑1 (MQ=255)  aaataaaataAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACt                                                                                                                                                                                                                                      >  1:66530/1‑251 (MQ=255)                    atatGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATa                                                                                                                                                                                                                      >  1:245948/1‑249 (MQ=255)                      atGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGAGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGtt                                                                                                                                                                                                                  >  1:442163/1‑251 (MQ=255)                                                 tttATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATNAGTTTCTTATCAGTTTCAGCCTTAAAgcta                                                                                                                                                                                        >  2:207560/1‑247 (MQ=255)                                                                gAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATAc                                                                                                                                                                        >  2:336892/1‑251 (MQ=255)                                                                    tATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGTTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATg                                                                                                                                                                    >  2:121689/1‑251 (MQ=255)                                                                                   cGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAg                                                                                                                                                      <  2:66881/250‑1 (MQ=255)                                                                                    gTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTGTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTg                                                                                                                                                    <  1:285200/251‑1 (MQ=255)                                                                                      ataCAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGNCGAATATACAATGACATTGCGAGGTAGTgg                                                                                                                                                   >  2:238490/1‑250 (MQ=255)                                                                                             aaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAAt                                                                                                                                           <  2:345224/251‑1 (MQ=255)                                                                                                 caTTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACg                                                                                                                                        >  1:279612/1‑250 (MQ=255)                                                                                                    tCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACga                                                                                                                                       >  1:391005/1‑248 (MQ=255)                                                                                                       aaCAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAAt                                                                                                                                 <  2:153928/251‑1 (MQ=255)                                                                                                             ttttttAACAACATCCATGATGCATTTACGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGCGGTAGTGGATTTGAATACGAAGAAATAccaat                                                                                                                           >  2:392838/1‑248 (MQ=255)                                                                                                              tttttCACAACATCCATCATGCATTTAAGAAACAAGATTGGATTTCGAATTATGATAACCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCAt                                                                                                                           >  1:297112/1‑250 (MQ=255)                                                                                                                         atccatcATGCATTTGAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACACAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGAGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATg                                                                                                                >  2:302755/1‑250 (MQ=255)                                                                                                                                   cATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGATGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtca                                                                                                     <  1:429552/251‑1 (MQ=255)                                                                                                                                        aaGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtcattcat                                                                                                <  2:196881/251‑1 (MQ=255)                                                                                                                                             aaaaGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGAGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtcagaaaaccttc                                                                                           >  2:141668/1‑241 (MQ=255)                                                                                                                                                            gAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAAt                                                                            >  1:319082/1‑251 (MQ=255)                                                                                                                                                                 aTGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATGGATAAAAGTGGTTATCAGCCGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCCTAAAGATAACGGTGCCGAATATACCATGACATTGCGCGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCg                                                                       >  1:255415/1‑251 (MQ=255)                                                                                                                                                                   gATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGtt                                                                     <  2:66530/251‑1 (MQ=255)                                                                                                                                                                                  ttGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTATCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACACATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGa                                                        <  1:336892/249‑1 (MQ=255)                                                                                                                                                                                  ttGAGGGAGTTATTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGTATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGACAAAAACAGACg                                                      >  2:159328/1‑251 (MQ=255)                                                                                                                                                                                    gAGGGAGTTCTTTCGTTGCGCAACACAGTATNATAAAAGGGGTTATCTGGTGCTTTCATTAAAAAATGAAACTTTTCTCGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGACGAATATACAATGGCATTGCGAGGTATTGGATTTTAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCAAGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACATACGAt                                                    <  2:203902/251‑1 (MQ=255)                                                                                                                                                                                       ggAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGt                                                 >  1:321933/1‑251 (MQ=255)                                                                                                                                                                                             cTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATaa                                                                                                         >  2:366137/1‑189 (MQ=255)                                                                                                                                                                                             cTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATaa                                                                                                         <  1:366137/189‑1 (MQ=255)                                                                                                                                                                                                 cGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTggg                                        <  1:45435/250‑1 (MQ=255)                                                                                                                                                                                                    tGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGatat                                    >  1:237261/1‑251 (MQ=255)                                                                                                                                                                                                          aCTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACa                              <  1:360277/251‑1 (MQ=255)                                                                                                                                                                                                                      aaaagtaataaTCAGGTGCTTTCATTAAACAATGACTCTGATCACGCCATGGATAGGATTGTTATCAGTATAAGCATAAAAGATCACGGTGCAGAATATACAATGACATTGCGCGGTAGTGGATTTGAATACGAAGAAATACCCAAAACAATAAATGAATATAACTCATTCATGGATGTAAAACACCGTGAATTACAGTTAGAACAAACCAGACGATTGTCTGCCTGGGATATTCTACAGACAAAACAGTc                  >  2:161814/11‑251 (MQ=255)                                                                                                                                                                                                                          gTGGTTATCAGGTGCTTTCATTAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc              >  1:249119/1‑251 (MQ=255)                                                                                                                                                                                                                                       tcTTTCATTAAAAAATGAAACGGTTCACTCNATGGATAAGTTTCTTATCAGTTGCAGCCGTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAATAAATACCCATAACAATAAATGAATATAACTCATTCAGGTATTTCAAAAACCGTGAATTTCCGTTAGAACAAAGCAGACGATTGTAAGCCGGGGATATTCTACATAAAAAACAGTCTGACATACCGAAAAgg  <  2:202684/249‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  CAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG  >  NC_000913/4437686‑4438165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑