Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	100.0%	179.6 / NA	54	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

CGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674283‑674762                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     cgCCAGTCCGGTCTGGTCGTGCGGGCCCCTGTTGACCGCAGAAGTCTTCTTATATTCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTTTTCCCAACGGTA‑GTATCCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCGCGGTGCCGGAGCGTTGTTGTTTTTCACCGCCACGCCTTCCgcaggc                                                                                                                                                                                                                                        <  1:99714/250‑1 (MQ=255) cgCCAGTACGGTCTGGTCGTTCGGGCACCAGGTGACGGCAGAAGTTTTCTTATATCCCAGGCTTTTTTTATCCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCAAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTAAT‑ATACGCGATTTTGTCGTACGCCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCgcaggc                                                                                                                                                                                                                                        <  1:195963/250‑1 (MQ=255)     aGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGCCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGGGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGAcc                                                                                                                                                                                                                                   <  1:49246/251‑1 (MQ=255)               gggcGTTCGGGCCCCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATCCAGCCCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATCCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGTTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcgcg                                                                                                                                                                                                                            <  1:272884/245‑1 (MQ=255)                        ggCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc                                                                                                                                                                                                                >  1:278935/1‑251 (MQ=255)                        ggCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGGTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc                                                                                                                                                                                                                >  1:320640/1‑251 (MQ=255)                        ggCACCAGTTGACCGCAAAAGCCTTTTTATATACCAGGCTTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAACCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATGCGCGATGTTGTCTTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc                                                                                                                                                                                                                <  1:102531/251‑1 (MQ=255)                              aGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGc                                                                                                                                                                                                          >  1:21468/1‑251 (MQ=255)                                 ggcccgcggatgtttttttATATCCCAGGCCTTTTTTATCCAGCTGGGTGAAGATTTTCGGTCCCAAACGGTA‑GGATCCCGGCGTAAAGTTGGCCAGCCCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTAAT‑ATACGCGATGTTGGCGTACGCCCCCGGGGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAg                                                                                                                                                                                                        <  2:278935/233‑1 (MQ=255)                                             tcttctTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAt                                                                                                                                                                                                                 >  1:322640/1‑229 (MQ=255)                                               ttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCac                                                                                                                                                                                         >  1:313772/1‑250 (MQ=255)                                                    atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATAc                                                                                                                                                                                    >  1:230323/1‑251 (MQ=255)                                                       tccagtgctttttttATCCAGCGCGGTGAAGAATTGCGGTTCCCAACGGTA‑TTATTCCGGCGTACAGGTTGCCAGCGCGCGGCTCAATTCATAACCAAA‑CCCATGCTTTTGG‑AGCTGGTTTTTCAT‑ATACGCGTTGTTGTCGTACGCCCAGGGTGCGGGAGCGGTGTTGTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGc                                                                                                                                                                                  <  2:111831/241‑1 (MQ=255)                                                        aCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTg                                                                                                                                                                                >  2:260488/1‑251 (MQ=255)                                                         ccAGGTCTTTTTTATACCGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTTTTCCGGCGTACAGTTTGCCAGCGCGCGGCTCCAGTCATAACAAAAGCCCA‑GCATTTTG‑TGCTGGTTTTTCAA‑TTCCGGGTTGTTTTCTTACGCCCACGGTGCCGGAGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTgg                                                                                                                                                                               <  1:431326/251‑1 (MQ=255)                                                              ccTTTTTTATCCAGCGCGGTGAAGAATTCCGGTTCCCATCGGTTTGT‑TCCCGGCGTACGGGGTGCCACCTCGCGCCCCAAGTCATCACCAAAGCCCA‑GCATTTTG‑CGCGGTTTTTTCAT‑CTCCGCGATGTTGTGGTACCTCCGCGGTGCGGGAGGGGTGTTGTTTTGCCCCGCCACGCCGTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCg                                                                                                                                                                          <  2:87895/251‑1 (MQ=255)                                                                  ttttATACAGCCCGTTGAAAAATTTCTGTTCCCAACGTTT‑GTATTCCGGCTTCCAGTTTGCCAGCTCGCTGCTCCATTAATACCCAAAGCCCA‑GCATTTTG‑ATCTGGTTTTTCAT‑ATACGCGATGTTGTCGAACGTCCCCGGTGCCGGAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGc                                                                                                                                                                       <  1:332009/250‑1 (MQ=255)                                                                           gCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                                                                                                                              >  1:52224/1‑250 (MQ=255)                                                                               ggTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                          >  1:348889/1‑250 (MQ=255)                                                                               ggTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                          >  1:417399/1‑250 (MQ=255)                                                                                 gaaaaaaTTTCTTTTCCCAACGGGA‑GAATCCCGGCTTACAGGTTGCCATCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTGG‑AGCGGGTTTTTCAT‑ATACGCGATGTTTTCGTACGCCCACGTTGCCGGAGCGGTGTGGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATg                                                                                                                                                       <  1:445402/246‑1 (MQ=255)                                                                                  aaaaaaTTTCTTTTCCCACTGGTA‑TTATCCCGGCGTACAGGTTGCCAGCTCGCGGCTCCGGTCATAACCAAGCCCCA‑GCATTTTG‑AGCTGTTTTTTCAT‑TTCCGGTATGTTGTCGTACGTCCACGTTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                                                                                                                      <  1:112933/247‑1 (MQ=255)                                                                                   aagaaTTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgt                                                                                                                                                     >  1:225165/1‑251 (MQ=255)                                                                                     gaaTTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGNTGGTAGCGGGCGCTCACGTCACCGATGgtgg                                                                                                                                                   >  2:214197/1‑250 (MQ=255)                                                                                        tttCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCACACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGAAGt                                                                                                                                                >  1:242697/1‑251 (MQ=255)                                                                                              ttCCCAACGGTA‑GTATTCCGGCGTAAGGGTTGCCAGCTCGGGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGCCCACGGTGCCGGAGGGTTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                                                                                                                           <  1:261381/250‑1 (MQ=255)                                                                                              tgcccAACGGTA‑GTTTTCCGGCGTACAGGTTGCAAGCTCGCGCATCCAGTCATACCAAAAGCCCA‑GCTTTTTG‑ATTGGGTTTTTCAT‑ATACGGGATGTTGTCGTACGTCCACGGTCCCGGAGGGGTTTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                                                                                                                           <  1:412865/248‑1 (MQ=255)                                                                                                cccAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑CGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacg                                                                                                                                        >  2:114426/1‑251 (MQ=255)                                                                                                cccAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacg                                                                                                                                        >  2:308302/1‑251 (MQ=255)                                                                                                                  ggCGTACGGGTGGCCGGCTCGGGGCTCCAGTCAAAACCAAAGCCCA‑GCATTTTG‑GGCGGGTTTTTCAT‑ATCCGCGATGTTGTCGTCCGCCCACGGGGCCGGTGCGGTTTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                                                                                                                       <  1:260488/251‑1 (MQ=255)                                                                                                                       ccggttggCCAGCCCGCGGTTCCAGTCATACCCAAGCCCCG‑GAATTTGG‑GGCTGTTTTTTCAT‑ATCCGTGTTTTTGCCGTCCGCCCACGGTGCGGGAGCGGTGTTGTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAg                                                                                                                  <  2:52224/244‑1 (MQ=255)                                                                                                                                ccagttcggggccccttaataaaccaaGCCCC‑GCATTTTGGAGCTGGTTTT‑CATTATCCGCATTTTTGCCGTACGCCCAGGGTGCCGGGGGGGTGTTTTTTTACCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAATGATAg                                                                                                          <  2:361542/226‑1 (MQ=255)                                                                                                                                           tCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTAAACGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGAc                                                                                              >  1:404273/1‑251 (MQ=255)                                                                                                                                            ccAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATCCGGGATTTTGTCGTCCGTCCCCGGTGCCGGAGCGGTGTTTTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGa                                                                                               <  1:154457/249‑1 (MQ=255)                                                                                                                                             cAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGCTAGGGGAGCATAGAc                                                                                              >  2:58213/1‑249 (MQ=255)                                                                                                                                                    aaCCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGAAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAgga                                                                                      >  1:432082/1‑248 (MQ=255)                                                                                                                                                      ccAAGGCCCA‑GCATTTTG‑AGTTGTTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCGCGGTGCCGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATa                                                                                   <  1:308302/251‑1 (MQ=255)                                                                                                                                                         aaGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACtt                                                                                >  1:64580/1‑251 (MQ=255)                                                                                                                                                                 gCATTTTG‑AGGTGGTTTTACAT‑ATACGCGTTTTTGGCGAACGCCCGGGGTGCGGGAGCGGGTTTGTTTGTCGCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTg                                                                         <  1:182284/251‑1 (MQ=255)                                                                                                                                                                            cTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCtt                                                               >  2:188537/1‑251 (MQ=255)                                                                                                                                                                                    tgaaaatgcgggttgttgtCGTAGGTCCACGTTGCCGGGGCGTTTTTTTTTTTCCCCGCCACTCCTTCCGCAGGCAGACCAAACTCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTa                                                         <  1:295414/237‑1 (MQ=255)                                                                                                                                                                                             gcgATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAAtgt                                               >  1:421949/1‑251 (MQ=255)                                                                                                                                                                                              cgATGTTGTCGTACGTACACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCCGTAATACTTCTNTTTGCTCTCGTCTTCGGTTCCTTCAAAtgtg                                              >  2:216352/1‑251 (MQ=255)                                                                                                                                                                                                 tgtggtcgtcCGCCCACGGGGCCGGGGCGTTGTTGTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgcg                                            <  2:196907/246‑1 (MQ=255)                                                                                                                                                                                                             ccccgggggcgttagggttgttgtTTTGCCCCGTCACGCCTTCCGCAGTATGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTGTTTGCCCAGCATTCGGTGGGAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTTCGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTGTTCTTTGCTCTCGTCTTCGGTTACATCAAATGTGCGCTTCTCATCCCaa                               <  2:411393/233‑1 (MQ=255)                                                                                                                                                                                                              ccgcggtgcgggggcgggtttgttTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTTCTTCAAATGTGCGTTTCTCATCCCAAt                              <  2:21468/232‑1 (MQ=255)                                                                                                                                                                                                                 cGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTATCTTTGCTCTCGTCTTCGGTTACTTCAACTGTGCGCTTCTCATCCCAATGa                            >  1:351355/1‑250 (MQ=255)                                                                                                                                                                                                                     cccgggggggTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGTCCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCGCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTg                       <  2:313772/243‑1 (MQ=255)                                                                                                                                                                                                                        ggAGCGGTGTTGTTTTTCACCGCCACGCCTTCAGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTAc                    >  1:141235/1‑251 (MQ=255)                                                                                                                                                                                                                            cGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGAGCGCTTCTCATCCCAATGAAGCTGTACTTTg                >  1:92302/1‑251 (MQ=255)                                                                                                                                                                                                                              ggtttgttTTTCCCCGCCACGCCGTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATTCTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa              <  2:143850/248‑1 (MQ=255)                                                                                                                                                                                                                               tgttgtTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt             >  1:449541/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttttcCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTa         <  1:319917/250‑1 (MQ=255)                                                                                                                                                                                                                                          aCCGCCACGCCTTCAGCAGGCAGACCAAACGCGTCCCAGCCGATAGGCTGCCGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGCGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGCCAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTTCTTCAAATGTGCGCTTCTACTCCCAATGAAGCTGTCCTTTGGATTCTATCTCTTc  >  2:279164/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTA‑GTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCA‑GCATTTTG‑AGCTGGTTTTTCAT‑ATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674283‑674762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑