Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,952,038	A→C	intergenic (+1481/‑163)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,952,038	0	A	C	100.0%	274.1 / NA	74	intergenic (+1481/‑163)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/39);  total (35/39)

GCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGT  >  NC_000913/3951794‑3952281                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    gcgcaAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCa                                                                                                                                                                                                                                               <  1:444950/248‑1 (MQ=255)     aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAaccc                                                                                                                                                                                                                                           >  1:212002/1‑249 (MQ=255)     aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAacac                                                                                                                                                                                                                                           >  2:245109/1‑251 (MQ=255)       gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCGGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                                                                                                         <  1:344985/251‑1 (MQ=255)       gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGCTTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                                                                                                         >  2:181369/1‑251 (MQ=255)              gACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCCACACATCACCCgt                                                                                                                                                                                                                                  >  2:372687/1‑250 (MQ=255)                  aaaCGATAACGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAg                                                                                                                                                                                                                              <  2:321977/250‑1 (MQ=255)                          ccGTCGTCTGTATCTCAGGTGACGGATCTTTCATGATGAATGTGCTAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAACCGTCTTACTCGACAACCAACGGTTAGGGCTGGTTCGACACTGGCAGCAACTGTTTTTCCAGGAAAGTTATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTACCCAGCGCCTTCGGCATACCTGGCCCACACATCACCCgtaacgccctggtt                                                                                                                                                                                                                      >  2:453661/1‑238 (MQ=255)                              cgTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGATCTGGGCACCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGaa                                                                                                                                                                                                                   <  2:181644/249‑1 (MQ=255)                                gcTGTATCTCCGGTGACGGCTCTTGCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTCCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCg                                                                                                                                                                                                                <  2:292621/250‑1 (MQ=255)                                 cTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCCCCCGTAAAGACCAGGTTGACGCgg                                                                                                                                                                                                               >  2:14059/1‑251 (MQ=255)                                   gtagctcCGTTGACGGCTCTATCATTATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACACGATAACCAACGGTTAGGGATGGTGCGACAATTGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCCCCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCa                                                                                                                                                                                                             <  2:148641/247‑1 (MQ=255)                                       cgccGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCtctc                                                                                                                                                                                                          <  1:351620/248‑1 (MQ=255)                                         ccGGTGACGGGTCTTTCAAGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCATGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGGAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAAGTTAGCCAGCGCCTTCGTCATCCCTGGCCGACACATCGCCCGTAAAGACCAGGTTGAAGCGGCACTCGa                                                                                                                                                                                                        <  1:11014/250‑1 (MQ=255)                                           ggTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTTAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCAAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGCAAAGACCAGGCTGAAGCGGCACtggccac                                                                                                                                                                                                     >  2:83067/1‑245 (MQ=255)                                            gTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCCCCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACAcc                                                                                                                                                                                                    <  1:9689/251‑1 (MQ=255)                                             tGACGGCTATTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTATCCAGCGCCTTCGGCATCCCTGGACAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGCCAcc                                                                                                                                                                                                    >  2:240266/1‑250 (MQ=255)                                                            gatgaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGAt                                                                                                                                                                                     <  1:415599/250‑1 (MQ=255)                                                               gactgtgCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGc                                                                                                                                                                                 >  1:92206/4‑251 (MQ=255)                                                               gaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATggg                                                                                                                                                                                  <  2:265118/250‑1 (MQ=255)                                                               gaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATggg                                                                                                                                                                                  <  1:166260/250‑1 (MQ=255)                                                                     gCAAGAGCTGTGCACCTTAACACGACAGCAGTGACCGTTGAAAATCGTCTTCCTCGATAACCATCGATTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGACCTATATAGCTAATCCCCCCTTACCGATACCCCCGATTTCCTCATCTTAGCCAGCGCCTTCGGCATCCCTAGCCAACACATCACCCCTAAAGTCCAGGTTGAAGCGGCACTCTGCACCAGGCTGAACAGCGATGGGTCAtgc                                                                                                                                                                            <  2:19825/250‑3 (MQ=255)                                                                     gCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATAcc                                                                                                                                                                           >  1:75927/1‑251 (MQ=255)                                                                       aaGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCGCTCGACGCCATGCTGAACAGTGATGGGCCATACCTg                                                                                                                                                                         <  1:380851/251‑1 (MQ=255)                                                                           gCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTc                                                                                                                                                                     <  2:171047/251‑1 (MQ=255)                                                                                    cGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCa                                                                                                                                                             >  1:46007/1‑250 (MQ=255)                                                                                     gTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTTAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAAt                                                                                                                                                           <  1:135572/251‑1 (MQ=255)                                                                                                 cAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTga                                                                                                                                               <  1:330203/251‑1 (MQ=255)                                                                                                   gTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGCCACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAAATTg                                                                                                                                                >  2:368949/1‑248 (MQ=255)                                                                                                     tACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAAc                                                                                                                                           >  1:44038/1‑251 (MQ=255)                                                                                                      aCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTATCCAGGAACGATATAGCGAAACCACACTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGATGAACAGTGATGGGCCATCCCTGCTTCATGTCTCAATCGACGAACTTGAGAACg                                                                                                                                          >  1:308279/1‑251 (MQ=255)                                                                                                         gTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGGTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGATAACGTCt                                                                                                                                       <  2:106543/251‑1 (MQ=255)                                                                                                                   gTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGt                                                                                                                             <  2:46007/251‑1 (MQ=255)                                                                                                                           cGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTgccgcc                                                                                                                       <  1:185370/249‑1 (MQ=255)                                                                                                                                 ccAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGAAAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGcc                                                                                                                >  1:466569/1‑250 (MQ=255)                                                                                                                                     cGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTaa                                                                                                           <  1:424243/251‑1 (MQ=255)                                                                                                                                        ttAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTc                                                                                                        >  1:63268/1‑251 (MQ=255)                                                                                                                                         tAGGGATGGTTCTACAATGGCAGCAATTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGTCGAACTTGAGAACGTCTGTCCGCTGGTGCCGCCTGGTGCCAGTAATTc                                                                                                        <  1:164291/250‑1 (MQ=255)                                                                                                                                          aGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAg                                                                                                      <  1:442034/251‑1 (MQ=255)                                                                                                                                                ggTTCGACAATGGCAGCAACTGTTTTTCCAGGACCGATATAGCGAAACCCCCCTTACCGATACCCCCGATTTCCTCATGTTAGCACGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGAAGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                                                                                                 <  2:378077/250‑1 (MQ=255)                                                                                                                                                ggTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                                                                                                 <  1:83067/250‑1 (MQ=255)                                                                                                                                                  ttCGACAATGGCAGCACCTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGGGCCGCCTGGTGCCAGTAATTCAGAAATGTTg                                                                                              >  2:291447/1‑251 (MQ=255)                                                                                                                                                        aaTGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCCTGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCNTGGTGCCAGTAATTCAGAAATGTTGGAGaa                                                                                         >  2:233085/1‑250 (MQ=255)                                                                                                                                                               gcaACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCat                                                                                 >  2:284272/1‑251 (MQ=255)                                                                                                                                                                caACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg                                                                                <  1:181369/251‑1 (MQ=255)                                                                                                                                                                 aaCTGTTTTTCCCGGAACGATATATCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCATGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatga                                                                               <  2:360699/251‑1 (MQ=255)                                                                                                                                                                 aaCTGTCTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCCTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGAGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg                                                                                >  2:360878/1‑250 (MQ=255)                                                                                                                                                                  aCTGGTTTTCCAGGAACGATATAGCGAAACCACCCTGACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatgat                                                                              <  1:239688/251‑1 (MQ=255)                                                                                                                                                                        tttccAGGAACGATATAGCGAAACCCCCCTNACCGATCACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCTGCACTCGACACCATGCTGAACAGTGAGGGGCCATACCTGCTTCAGGGCTCAATCGACGAACTTGAGAACGTCTGGACGCTGGTTCCGCCTGGTGCCAGTAATTCAGAAAGGTTGGAGATATTATCATGATGCAAc                                                                         <  2:215738/246‑1 (MQ=255)                                                                                                                                                                        tttccAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAAc                                                                         >  2:71730/5‑250 (MQ=255)                                                                                                                                                                          tccAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAt                                                                       <  2:44038/248‑1 (MQ=255)                                                                                                                                                                                      ataGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGANATTATCATGATGCAACATCAGGTCAATGTAt                                                          >  2:206531/3‑251 (MQ=255)                                                                                                                                                                                        aGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCg                                                        >  1:246891/1‑251 (MQ=255)                                                                                                                                                                                        aGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCg                                                        >  1:14121/1‑251 (MQ=255)                                                                                                                                                                                         gCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGCGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCgg                                                       >  1:267859/1‑251 (MQ=255)                                                                                                                                                                                         gCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCgg                                                       >  1:378010/1‑251 (MQ=255)                                                                                                                                                                                          cGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGc                                                      <  1:390988/251‑1 (MQ=255)                                                                                                                                                                                              accaccCTTACCGATAACCCCGATTTCCGCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGc                                                  <  2:246891/251‑1 (MQ=255)                                                                                                                                                                                                caccCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCtt                                                >  1:275287/1‑251 (MQ=255)                                                                                                                                                                                                accccTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCCGGCTGAAGCGGCACTCGACACCATCCTCCACAGTGATGGGCCATCCCTGCTTCATGTCTCCATCGACGACCTTCAGAACGTCTTGCCGCTGGTGCCGCCTGGTGCCCGTAATTCCGCACTGTTCGAGTAATTATCAAACTGCAACAGCATGTCAATTTATcacctctctt                                                >  1:275290/3‑242 (MQ=255)                                                                                                                                                                                                  cccTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTc                                               >  1:178176/1‑250 (MQ=255)                                                                                                                                                                                                   ccTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCaa                                             <  1:71730/251‑1 (MQ=255)                                                                                                                                                                                                    cTTACCGATAACCCCGATGTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAAt                                            >  2:394766/1‑251 (MQ=255)                                                                                                                                                                                                       accgATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATcc                                          >  1:2147/4‑250 (MQ=255)                                                                                                                                                                                                        ccgATAACCCCGATTGCCTCATGTTAGCCAGCGCCTTCGGCATACCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGGGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGGGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAg                                        <  2:293031/249‑1 (MQ=255)                                                                                                                                                                                                             aaCCCCGATTTCCTCATGTTAGCCAGCGCCATCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACGGTGATGGGACATACCTGCTTCATGTTTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGACTGGTGCCAGTAATTCAGAAATGTTGGATAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAAc                                    <  1:451918/250‑1 (MQ=255)                                                                                                                                                                                                                      ttCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCACTCCAGAAACCTTAGCACgg                          >  1:430257/1‑250 (MQ=255)                                                                                                                                                                                                                          tCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACAAGGTTGAAGCGGCACTCGACACCATGCTGCACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGCGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTAACTCCAGAAAACTTaaaccgtggtt                      >  1:264551/1‑241 (MQ=255)                                                                                                                                                                                                                                  gCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAAACTTAGAACGTGATTTACGCgtg              >  1:280844/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgtggt            <  1:14059/251‑1 (MQ=255)                                                                                                                                                                                                                                     aGCGCCTTCGGCATCCCTGTCCAACACATCCCCCGTGAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTTATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgtggtg           <  1:308070/251‑1 (MQ=255)                                                                                                                                                                                                                                     aGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAAAGTGTTTTACGCgtggtg           >  1:356632/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggCATCCCTGGCCAACACATCTCCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCTTTCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGCACCTCTGGCCGCTGGTGCCGCCTGGTTCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGTCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGt  <  1:322701/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGt  <  1:368949/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGT  >  NC_000913/3951794‑3952281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑