Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,976	C→T	intergenic (+1419/‑225)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,976	0	C	T	100.0%	209.3 / NA	63	intergenic (+1419/‑225)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/35);  total (28/35)

CGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT  >  NC_000913/3951729‑3952212                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                                                                                                                                                                           <  1:94589/251‑1 (MQ=255) cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                                                                                                                                                                           <  2:353027/251‑1 (MQ=255)      aaTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGACGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGATCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAAACAAAGGTTAGGGAAGGTTTGGAAATGGCAGCAACTGGTTTTCCAGGAACGATATAGCGAAac                                                                                                                                                                                                                                      >  1:57143/1‑251 (MQ=255)                      gCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAGCTGTTTTTCCAGGAACGATATAGCGAAACCTCCCTTACCGATAAc                                                                                                                                                                                                                      <  2:435061/251‑1 (MQ=255)                       cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAcc                                                                                                                                                                                                                     <  2:1882/247‑1 (MQ=255)                       cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTATGTATCTCCGGTGCCGGGTCTTTCATGGAGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGAAATTACCGTTGAAAAACGTCTTACTCGATAAACCACGGTTAGGGATGGCTCGACAATGGCAGCAACTGGTTTTCCAGGAACGATATAGCGAAACCACCCCTAACGATAAcc                                                                                                                                                                                                                     >  1:378077/5‑251 (MQ=255)                            tAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCCCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAAATGTTTTTCCCGGAACGATATAGCGGAACCACCCTTACCGATAACCCCGAt                                                                                                                                                                                                                >  2:360661/1‑251 (MQ=255)                             aggcaCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAAGATACCGTGGACTGTATCTCCGGAGACGGCTCTTTCATGATGAATGAGCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAATTGTCTTACTCGATAACCAAAGGTTAGGGGTGGTTCGACAATGGCTGCCACTGTTTTTCCAGGCACGATATAGCGAAACCAACCTTACCGATAACCCCGAtt                                                                                                                                                                                                               >  1:292621/5‑251 (MQ=255)                                            tGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGcc                                                                                                                                                                                                 <  1:5051/250‑1 (MQ=255)                                                              tGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATccctg                                                                                                                                                                              >  1:261063/1‑248 (MQ=255)                                                              tGGCGCGCAAGTCGCGCGACCAAACGACCCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTCCTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGACCGATATAGCGAAACCACCCTTACCGATCACCCCGATTTCCTCATGTTCGCCAGCGCCTTCGGCATccctg                                                                                                                                                                              >  2:28580/1‑248 (MQ=255)                                                                  gcgcaAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCa                                                                                                                                                                          <  1:444950/248‑1 (MQ=255)                                                                      aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAaccc                                                                                                                                                                      >  1:212002/1‑249 (MQ=255)                                                                      aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAacac                                                                                                                                                                      >  2:245109/1‑251 (MQ=255)                                                                        gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCGGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                                    <  1:344985/251‑1 (MQ=255)                                                                        gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGCTTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                                    >  2:181369/1‑251 (MQ=255)                                                                               gACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCCACACATCACCCgt                                                                                                                                                             >  2:372687/1‑250 (MQ=255)                                                                               gACCAAACGATACCGTCGTCTGTATCTACGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGAATcggaagagcgtcgtgtaggga                                                                                                                                                              >  2:288006/1‑230 (MQ=255)                                                                                   aaaCGATAACGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAg                                                                                                                                                         <  2:321977/250‑1 (MQ=255)                                                                                           ccGTCGTCTGTATCTCAGGTGACGGATCTTTCATGATGAATGTGCTAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAACCGTCTTACTCGACAACCAACGGTTAGGGCTGGTTCGACACTGGCAGCAACTGTTTTTCCAGGAAAGTTATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTACCCAGCGCCTTCGGCATACCTGGCCCACACATCACCCgtaacgccctggtt                                                                                                                                                 >  2:453661/1‑238 (MQ=255)                                                                                               cgTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGATCTGGGCACCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGaa                                                                                                                                              <  2:181644/249‑1 (MQ=255)                                                                                                 gcTGTATCTCCGGTGACGGCTCTTGCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTCCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCg                                                                                                                                           <  2:292621/250‑1 (MQ=255)                                                                                                  cTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCCCCCGTAAAGACCAGGTTGACGCgg                                                                                                                                          >  2:14059/1‑251 (MQ=255)                                                                                                    gtagctcCGTTGACGGCTCTATCATTATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACACGATAACCAACGGTTAGGGATGGTGCGACAATTGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCCCCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCa                                                                                                                                        <  2:148641/247‑1 (MQ=255)                                                                                                        cgccGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCtctc                                                                                                                                     <  1:351620/248‑1 (MQ=255)                                                                                                          ccGGTGACGGGTCTTTCAAGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCATGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGGAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAAGTTAGCCAGCGCCTTCGTCATCCCTGGCCGACACATCGCCCGTAAAGACCAGGTTGAAGCGGCACTCGa                                                                                                                                   <  1:11014/250‑1 (MQ=255)                                                                                                            ggTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTTAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCAAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGCAAAGACCAGGCTGAAGCGGCACtggccac                                                                                                                                >  2:83067/1‑245 (MQ=255)                                                                                                             gTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCCCCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACAcc                                                                                                                               <  1:9689/251‑1 (MQ=255)                                                                                                              tGACGGCTATTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTATCCAGCGCCTTCGGCATCCCTGGACAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGCCAcc                                                                                                                               >  2:240266/1‑250 (MQ=255)                                                                                                                             gatgaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGAt                                                                                                                <  1:415599/250‑1 (MQ=255)                                                                                                                                gactgtgCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGc                                                                                                            >  1:92206/4‑251 (MQ=255)                                                                                                                                gaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATggg                                                                                                             <  2:265118/250‑1 (MQ=255)                                                                                                                                gaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATggg                                                                                                             <  1:166260/250‑1 (MQ=255)                                                                                                                                      gCAAGAGCTGTGCACCTTAACACGACAGCAGTGACCGTTGAAAATCGTCTTCCTCGATAACCATCGATTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGACCTATATAGCTAATCCCCCCTTACCGATACCCCCGATTTCCTCATCTTAGCCAGCGCCTTCGGCATCCCTAGCCAACACATCACCCCTAAAGTCCAGGTTGAAGCGGCACTCTGCACCAGGCTGAACAGCGATGGGTCAtgc                                                                                                       <  2:19825/250‑3 (MQ=255)                                                                                                                                      gCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATAcc                                                                                                      >  1:75927/1‑251 (MQ=255)                                                                                                                                        aaGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCGCTCGACGCCATGCTGAACAGTGATGGGCCATACCTg                                                                                                    <  1:380851/251‑1 (MQ=255)                                                                                                                                            gCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTc                                                                                                <  2:171047/251‑1 (MQ=255)                                                                                                                                                     cGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCa                                                                                        >  1:46007/1‑250 (MQ=255)                                                                                                                                                      gTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTTAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAAt                                                                                      <  1:135572/251‑1 (MQ=255)                                                                                                                                                                  cAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTga                                                                          <  1:330203/251‑1 (MQ=255)                                                                                                                                                                    gTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGCCACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAAATTg                                                                           >  2:368949/1‑248 (MQ=255)                                                                                                                                                                      tACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAAc                                                                      >  1:44038/1‑251 (MQ=255)                                                                                                                                                                       aCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTATCCAGGAACGATATAGCGAAACCACACTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGATGAACAGTGATGGGCCATCCCTGCTTCATGTCTCAATCGACGAACTTGAGAACg                                                                     >  1:308279/1‑251 (MQ=255)                                                                                                                                                                          gTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGGTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGATAACGTCt                                                                  <  2:106543/251‑1 (MQ=255)                                                                                                                                                                                    gTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGt                                                        <  2:46007/251‑1 (MQ=255)                                                                                                                                                                                            cGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTgccgcc                                                  <  1:185370/249‑1 (MQ=255)                                                                                                                                                                                                  ccAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGAAAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGcc                                           >  1:466569/1‑250 (MQ=255)                                                                                                                                                                                                      cGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTaa                                      <  1:424243/251‑1 (MQ=255)                                                                                                                                                                                                         ttAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTc                                   >  1:63268/1‑251 (MQ=255)                                                                                                                                                                                                          tAGGGATGGTTCTACAATGGCAGCAATTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGTCGAACTTGAGAACGTCTGTCCGCTGGTGCCGCCTGGTGCCAGTAATTc                                   <  1:164291/250‑1 (MQ=255)                                                                                                                                                                                                           aGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAg                                 <  1:442034/251‑1 (MQ=255)                                                                                                                                                                                                                 ggTTCGACAATGGCAGCAACTGTTTTTCCAGGACCGATATAGCGAAACCCCCCTTACCGATACCCCCGATTTCCTCATGTTAGCACGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGAAGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                            <  2:378077/250‑1 (MQ=255)                                                                                                                                                                                                                 ggTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                            <  1:83067/250‑1 (MQ=255)                                                                                                                                                                                                                   ttCGACAATGGCAGCACCTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGGGCCGCCTGGTGCCAGTAATTCAGAAATGTTg                         >  2:291447/1‑251 (MQ=255)                                                                                                                                                                                                                         aaTGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCCTGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCNTGGTGCCAGTAATTCAGAAATGTTGGAGaa                    >  2:233085/1‑250 (MQ=255)                                                                                                                                                                                                                                gcaACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCat            >  2:284272/1‑251 (MQ=255)                                                                                                                                                                                                                                 caACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg           <  1:181369/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaCTGTTTTTCCCGGAACGATATATCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCATGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatga          <  2:360699/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaCTGTCTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCCTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGAGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg           >  2:360878/1‑250 (MQ=255)                                                                                                                                                                                                                                   aCTGGTTTTCCAGGAACGATATAGCGAAACCACCCTGACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatgat         <  1:239688/251‑1 (MQ=255)                                                                                                                                                                                                                                         tttccAGGAACGATATAGCGAAACCCCCCTNACCGATCACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCTGCACTCGACACCATGCTGAACAGTGAGGGGCCATACCTGCTTCAGGGCTCAATCGACGAACTTGAGAACGTCTGGACGCTGGTTCCGCCTGGTGCCAGTAATTCAGAAAGGTTGGAGATATTATCATGATGCAAc    <  2:215738/246‑1 (MQ=255)                                                                                                                                                                                                                                         tttccAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAAc    >  2:71730/5‑250 (MQ=255)                                                                                                                                                                                                                                           tccAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAt  <  2:44038/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             CGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT  >  NC_000913/3951729‑3952212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑