Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,964	T→C	intergenic (+1407/‑237)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,964	0	T	C	100.0%	219.3 / NA	61	intergenic (+1407/‑237)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/34);  total (27/34)

CACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGAT  >  NC_000913/3951721‑3952205                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cacTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAAc                                                                                                                                                                                                                                            <  2:75677/251‑1 (MQ=255)         cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                                                                                                                                                                    <  1:94589/251‑1 (MQ=255)         cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                                                                                                                                                                    <  2:353027/251‑1 (MQ=255)              aaTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGACGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGATCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAAACAAAGGTTAGGGAAGGTTTGGAAATGGCAGCAACTGGTTTTCCAGGAACGATATAGCGAAac                                                                                                                                                                                                                               >  1:57143/1‑251 (MQ=255)                              gCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAGCTGTTTTTCCAGGAACGATATAGCGAAACCTCCCTTACCGATAAc                                                                                                                                                                                                               <  2:435061/251‑1 (MQ=255)                               cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAcc                                                                                                                                                                                                              <  2:1882/247‑1 (MQ=255)                               cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTATGTATCTCCGGTGCCGGGTCTTTCATGGAGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGAAATTACCGTTGAAAAACGTCTTACTCGATAAACCACGGTTAGGGATGGCTCGACAATGGCAGCAACTGGTTTTCCAGGAACGATATAGCGAAACCACCCCTAACGATAAcc                                                                                                                                                                                                              >  1:378077/5‑251 (MQ=255)                                    tAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCCCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAAATGTTTTTCCCGGAACGATATAGCGGAACCACCCTTACCGATAACCCCGAt                                                                                                                                                                                                         >  2:360661/1‑251 (MQ=255)                                     aggcaCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAAGATACCGTGGACTGTATCTCCGGAGACGGCTCTTTCATGATGAATGAGCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAATTGTCTTACTCGATAACCAAAGGTTAGGGGTGGTTCGACAATGGCTGCCACTGTTTTTCCAGGCACGATATAGCGAAACCAACCTTACCGATAACCCCGAtt                                                                                                                                                                                                        >  1:292621/5‑251 (MQ=255)                                                    tGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGcc                                                                                                                                                                                          <  1:5051/250‑1 (MQ=255)                                                                      tGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATccctg                                                                                                                                                                       >  1:261063/1‑248 (MQ=255)                                                                      tGGCGCGCAAGTCGCGCGACCAAACGACCCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTCCTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGACCGATATAGCGAAACCACCCTTACCGATCACCCCGATTTCCTCATGTTCGCCAGCGCCTTCGGCATccctg                                                                                                                                                                       >  2:28580/1‑248 (MQ=255)                                                                          gcgcaAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCa                                                                                                                                                                   <  1:444950/248‑1 (MQ=255)                                                                              aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAaccc                                                                                                                                                               >  1:212002/1‑249 (MQ=255)                                                                              aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAacac                                                                                                                                                               >  2:245109/1‑251 (MQ=255)                                                                                gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCGGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                             <  1:344985/251‑1 (MQ=255)                                                                                gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGCTTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                             >  2:181369/1‑251 (MQ=255)                                                                                       gACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCCACACATCACCCgt                                                                                                                                                      >  2:372687/1‑250 (MQ=255)                                                                                       gACCAAACGATACCGTCGTCTGTATCTACGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGAATcggaagagcgtcgtgtaggga                                                                                                                                                       >  2:288006/1‑230 (MQ=255)                                                                                           aaaCGATAACGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAg                                                                                                                                                  <  2:321977/250‑1 (MQ=255)                                                                                                   ccGTCGTCTGTATCTCAGGTGACGGATCTTTCATGATGAATGTGCTAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAACCGTCTTACTCGACAACCAACGGTTAGGGCTGGTTCGACACTGGCAGCAACTGTTTTTCCAGGAAAGTTATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTACCCAGCGCCTTCGGCATACCTGGCCCACACATCACCCgtaacgccctggtt                                                                                                                                          >  2:453661/1‑238 (MQ=255)                                                                                                       cgTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGATCTGGGCACCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGaa                                                                                                                                       <  2:181644/249‑1 (MQ=255)                                                                                                         gcTGTATCTCCGGTGACGGCTCTTGCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTCCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCg                                                                                                                                    <  2:292621/250‑1 (MQ=255)                                                                                                          cTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCCCCCGTAAAGACCAGGTTGACGCgg                                                                                                                                   >  2:14059/1‑251 (MQ=255)                                                                                                            gtagctcCGTTGACGGCTCTATCATTATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACACGATAACCAACGGTTAGGGATGGTGCGACAATTGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCCCCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCa                                                                                                                                 <  2:148641/247‑1 (MQ=255)                                                                                                                cgccGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCtctc                                                                                                                              <  1:351620/248‑1 (MQ=255)                                                                                                                  ccGGTGACGGGTCTTTCAAGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCATGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGGAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAAGTTAGCCAGCGCCTTCGTCATCCCTGGCCGACACATCGCCCGTAAAGACCAGGTTGAAGCGGCACTCGa                                                                                                                            <  1:11014/250‑1 (MQ=255)                                                                                                                    ggTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTTAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCAAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGCAAAGACCAGGCTGAAGCGGCACtggccac                                                                                                                         >  2:83067/1‑245 (MQ=255)                                                                                                                     gTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCCCCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACAcc                                                                                                                        <  1:9689/251‑1 (MQ=255)                                                                                                                      tGACGGCTATTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTATCCAGCGCCTTCGGCATCCCTGGACAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGCCAcc                                                                                                                        >  2:240266/1‑250 (MQ=255)                                                                                                                                     gatgaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGAt                                                                                                         <  1:415599/250‑1 (MQ=255)                                                                                                                                        gactgtgCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGc                                                                                                     >  1:92206/4‑251 (MQ=255)                                                                                                                                        gaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATggg                                                                                                      <  1:166260/250‑1 (MQ=255)                                                                                                                                        gaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATggg                                                                                                      <  2:265118/250‑1 (MQ=255)                                                                                                                                              gCAAGAGCTGTGCACCTTAACACGACAGCAGTGACCGTTGAAAATCGTCTTCCTCGATAACCATCGATTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGACCTATATAGCTAATCCCCCCTTACCGATACCCCCGATTTCCTCATCTTAGCCAGCGCCTTCGGCATCCCTAGCCAACACATCACCCCTAAAGTCCAGGTTGAAGCGGCACTCTGCACCAGGCTGAACAGCGATGGGTCAtgc                                                                                                <  2:19825/250‑3 (MQ=255)                                                                                                                                              gCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATAcc                                                                                               >  1:75927/1‑251 (MQ=255)                                                                                                                                                aaGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCGCTCGACGCCATGCTGAACAGTGATGGGCCATACCTg                                                                                             <  1:380851/251‑1 (MQ=255)                                                                                                                                                    gCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTc                                                                                         <  2:171047/251‑1 (MQ=255)                                                                                                                                                             cGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCa                                                                                 >  1:46007/1‑250 (MQ=255)                                                                                                                                                              gTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTTAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAAt                                                                               <  1:135572/251‑1 (MQ=255)                                                                                                                                                                          cAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTga                                                                   <  1:330203/251‑1 (MQ=255)                                                                                                                                                                            gTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGCCACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAAATTg                                                                    >  2:368949/1‑248 (MQ=255)                                                                                                                                                                              tACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAAc                                                               >  1:44038/1‑251 (MQ=255)                                                                                                                                                                               aCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTATCCAGGAACGATATAGCGAAACCACACTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGATGAACAGTGATGGGCCATCCCTGCTTCATGTCTCAATCGACGAACTTGAGAACg                                                              >  1:308279/1‑251 (MQ=255)                                                                                                                                                                                  gTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGGTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGATAACGTCt                                                           <  2:106543/251‑1 (MQ=255)                                                                                                                                                                                            gTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGt                                                 <  2:46007/251‑1 (MQ=255)                                                                                                                                                                                                    cGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTgccgcc                                           <  1:185370/249‑1 (MQ=255)                                                                                                                                                                                                          ccAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGAAAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGcc                                    >  1:466569/1‑250 (MQ=255)                                                                                                                                                                                                              cGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTaa                               <  1:424243/251‑1 (MQ=255)                                                                                                                                                                                                                 ttAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTc                            >  1:63268/1‑251 (MQ=255)                                                                                                                                                                                                                  tAGGGATGGTTCTACAATGGCAGCAATTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGTCGAACTTGAGAACGTCTGTCCGCTGGTGCCGCCTGGTGCCAGTAATTc                            <  1:164291/250‑1 (MQ=255)                                                                                                                                                                                                                   aGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAg                          <  1:442034/251‑1 (MQ=255)                                                                                                                                                                                                                         ggTTCGACAATGGCAGCAACTGTTTTTCCAGGACCGATATAGCGAAACCCCCCTTACCGATACCCCCGATTTCCTCATGTTAGCACGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGAAGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                     <  2:378077/250‑1 (MQ=255)                                                                                                                                                                                                                         ggTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                     <  1:83067/250‑1 (MQ=255)                                                                                                                                                                                                                           ttCGACAATGGCAGCACCTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGGGCCGCCTGGTGCCAGTAATTCAGAAATGTTg                  >  2:291447/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaTGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCCTGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCNTGGTGCCAGTAATTCAGAAATGTTGGAGaa             >  2:233085/1‑250 (MQ=255)                                                                                                                                                                                                                                        gcaACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCat     >  2:284272/1‑251 (MQ=255)                                                                                                                                                                                                                                         caACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg    <  1:181369/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaCTGTTTTTCCCGGAACGATATATCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCATGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatga   <  2:360699/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaCTGTCTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCCTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGAGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg    >  2:360878/1‑250 (MQ=255)                                                                                                                                                                                                                                           aCTGGTTTTCCAGGAACGATATAGCGAAACCACCCTGACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatgat  <  1:239688/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGAT  >  NC_000913/3951721‑3952205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑