Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,823 T→C intergenic (+1266/‑378) ilvX → / → ilvM uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,8230TC98.1% 162.8 / ‑6.0 53intergenic (+1266/‑378)ilvX/ilvMuncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (22/30);  minor base G (1/0);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTT  >  NC_000913/3951581‑3952069
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      
gccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTTTCAGCTCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTAt                                                                                                                                                                                                                                                <  2:128345/250‑1 (MQ=255)
          aCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGa                                                                                                                                                                                                                                       <  1:333023/250‑1 (MQ=255)
             aCCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGc                                                                                                                                                                                                                                   <  2:288500/251‑1 (MQ=255)
              ccATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGct                                                                                                                                                                                                                                  <  1:73134/251‑1 (MQ=255)
               cATCCCTGTGACTCTAGCTACTCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGTATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGTGCTGCGCAGCACATCGCCCACTCTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATTCCGTCGTCTGTATCTCCGGTGACGGctc                                                                                                                                                                                                                                 <  1:405446/251‑1 (MQ=255)
               cATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACCATACCGTCGTCTGGATCTCCGGGGACGGctc                                                                                                                                                                                                                                 >  1:13309/1‑251 (MQ=255)
                 ccccGGTGACGCTATCTACGCGCCGTGGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCtt                                                                                                                                                                                                                               <  2:137377/250‑1 (MQ=255)
                                    gcgcCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAaga                                                                                                                                                                                                            <  1:438845/251‑1 (MQ=255)
                                                             cagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAACTCGCGCGACCAAACGATACCGTCATCTGTATCTCCGGTGCAGGCTCTTTCATGACGACTGTGCACGAGCTTGGCCCCCTAAAACGCCAGccg                                                                                                                                                                                   >  2:13808/3‑249 (MQ=255)
                                                                      aaCCTGCGGATTGCGTCGTGACCACAGATGTNGTGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGGTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTGCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTg                                                                                                                                                                          <  2:214982/251‑1 (MQ=255)
                                                                           gCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGaaaa                                                                                                                                                                      <  2:78025/250‑1 (MQ=255)
                                                                            cGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACCCAAGCCGATACCGGTGAAAAtt                                                                                                                                                                    >  2:5051/1‑250 (MQ=255)
                                                                            cGGATGGCGGCGTGACCACAGATTGGGGGCAGCCCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCGGTATCTCCGGTGACGGCGCTTTCATGATGAATGTGCAAGAGCTGGGAACCGTAAAACGCAAGCAGTTACCGTTGAAAATc                                                                                                                                                                    <  2:179799/251‑1 (MQ=255)
                                                                                   cgtcgtTACCACAGATGTGGGGCAGCAACAGATGTGGGCTGCGCAGCACATCGCCCCCACTCGCCCGGAAAATTTCATCACCGCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTAc                                                                                                                                                             <  1:466475/251‑1 (MQ=255)
                                                                                      cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTc                                                                                                                                                           <  2:326027/250‑1 (MQ=255)
                                                                                      cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCGCTTTCATGATGAATGTGCAAGAGCTGGGCACCGAAAAACGCCAGCAGTTACCGTTGAAAATCGGCTTACTCg                                                                                                                                                          >  1:293031/1‑251 (MQ=255)
                                                                                       gtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCg                                                                                                                                                          >  1:75677/1‑250 (MQ=255)
                                                                                                 aTGTGGGGCAGCACCAGATGTGGGCTGCGCAGCCCATCGCCCCCACCCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACgg                                                                                                                                               <  2:344955/251‑1 (MQ=255)
                                                                                                   gtgGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGtt                                                                                                                                             <  1:445178/251‑1 (MQ=255)
                                                                                                       ggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATGTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAggg                                                                                                                                         <  2:72290/251‑1 (MQ=255)
                                                                                                        gcagcaCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGa                                                                                                                                        <  1:133247/251‑1 (MQ=255)
                                                                                                         cagcaCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGGTCCCGTTGAAAATCGTCTTACTCGATAACCCACGGGTAGGGAt                                                                                                                                       >  1:265118/1‑251 (MQ=255)
                                                                                                            caCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGGTAGGGATggg                                                                                                                                    >  2:390988/1‑250 (MQ=255)
                                                                                                                           gcgcAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGgcag                                                                                                                       <  1:7878/249‑1 (MQ=255)
                                                                                                                            cgcAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGGGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCCTGATGAATGTGCAAGAGATGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTAATCGATAACCAACGGTTAGGGATGGttaggccaaggcagaa                                                                                                                     >  1:181644/1‑236 (MQ=255)
                                                                                                                              cagcaCATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACt                                                                                                                  <  2:403027/251‑1 (MQ=255)
                                                                                                                                 cacaTCGCACACCCTCGCCCGGAAAAGTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGCCGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGtt                                                                                                               <  1:75876/251‑1 (MQ=255)
                                                                                                                                  ccaTCGCCCACCCCCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCTCCATGGTTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGCCGCGCGAGCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTGTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAAGCGTCTTACTCGATAACCAACGGTTAGGGAGGGTTCGACAATGGCAGCAACTGttt                                                                                                              <  2:24200/250‑1 (MQ=255)
                                                                                                                                            cacTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAAc                                                                                                    <  2:75677/251‑1 (MQ=255)
                                                                                                                                                  cccGGAAAATTTCATCACATCCAGCGGCTTAGGCACAATGGGTTTTGGTTTAACGACGGCGGACGGCGCGAAAGTCGCGCGACCCACCGACACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGCATGTGCAAAGGCTCGGCACCGTAAAACGCAAGCAGATAACGTTAAAAATCGTCTTAATCGATAACCAACGGCTAGGGATGGTTCGACAAAGGCAGCCCCTGtttttcccggacagcaaa                                                                                               >  2:407787/1‑237 (MQ=255)
                                                                                                                                                   ccGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCAACGTAAAAAGCAAGCAGTTACCGTTTAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGgcggcacatgattttcccggaacgagat                                                                                               >  1:95509/1‑227 (MQ=255)
                                                                                                                                                    cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                            <  1:94589/251‑1 (MQ=255)
                                                                                                                                                    cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                            <  2:353027/251‑1 (MQ=255)
                                                                                                                                                         aaTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGACGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGATCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAAACAAAGGTTAGGGAAGGTTTGGAAATGGCAGCAACTGGTTTTCCAGGAACGATATAGCGAAac                                                                                       >  1:57143/1‑251 (MQ=255)
                                                                                                                                                                         gCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAGCTGTTTTTCCAGGAACGATATAGCGAAACCTCCCTTACCGATAAc                                                                       <  2:435061/251‑1 (MQ=255)
                                                                                                                                                                          cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAcc                                                                      <  2:1882/247‑1 (MQ=255)
                                                                                                                                                                          cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTATGTATCTCCGGTGCCGGGTCTTTCATGGAGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGAAATTACCGTTGAAAAACGTCTTACTCGATAAACCACGGTTAGGGATGGCTCGACAATGGCAGCAACTGGTTTTCCAGGAACGATATAGCGAAACCACCCCTAACGATAAcc                                                                      >  1:378077/5‑251 (MQ=255)
                                                                                                                                                                               tAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCCCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAAATGTTTTTCCCGGAACGATATAGCGGAACCACCCTTACCGATAACCCCGAt                                                                 >  2:360661/1‑251 (MQ=255)
                                                                                                                                                                                aggcaCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAAGATACCGTGGACTGTATCTCCGGAGACGGCTCTTTCATGATGAATGAGCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAATTGTCTTACTCGATAACCAAAGGTTAGGGGTGGTTCGACAATGGCTGCCACTGTTTTTCCAGGCACGATATAGCGAAACCAACCTTACCGATAACCCCGAtt                                                                >  1:292621/5‑251 (MQ=255)
                                                                                                                                                                                               tGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGcc                                                  <  1:5051/250‑1 (MQ=255)
                                                                                                                                                                                                                 tGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATccctg                               >  1:261063/1‑248 (MQ=255)
                                                                                                                                                                                                                 tGGCGCGCAAGTCGCGCGACCAAACGACCCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTCCTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGACCGATATAGCGAAACCACCCTTACCGATCACCCCGATTTCCTCATGTTCGCCAGCGCCTTCGGCATccctg                               >  2:28580/1‑248 (MQ=255)
                                                                                                                                                                                                                     gcgcaAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCa                           <  1:444950/248‑1 (MQ=255)
                                                                                                                                                                                                                         aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAaccc                       >  1:212002/1‑249 (MQ=255)
                                                                                                                                                                                                                         aaGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAacac                       >  2:245109/1‑251 (MQ=255)
                                                                                                                                                                                                                           gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCGGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                     <  1:344985/251‑1 (MQ=255)
                                                                                                                                                                                                                           gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGCTTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                     >  2:181369/1‑251 (MQ=255)
                                                                                                                                                                                                                                  gACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATaa                                                                                                                                                      >  2:213064/1‑115 (MQ=255)
                                                                                                                                                                                                                                  gACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATaa                                                                                                                                                      <  1:213064/115‑1 (MQ=255)
                                                                                                                                                                                                                                  gACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCCACACATCACCCgt              >  2:372687/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gACCAAACGATACCGTCGTCTGTATCTACGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGAATcggaagagcgtcgtgtaggga               >  2:288006/1‑230 (MQ=255)
                                                                                                                                                                                                                                      aaaCGATAACGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAg          <  2:321977/250‑1 (MQ=255)
                                                                                                                                                                                                                                              ccGTCGTCTGTATCTCAGGTGACGGATCTTTCATGATGAATGTGCTAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAACCGTCTTACTCGACAACCAACGGTTAGGGCTGGTTCGACACTGGCAGCAACTGTTTTTCCAGGAAAGTTATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTACCCAGCGCCTTCGGCATACCTGGCCCACACATCACCCgtaacgccctggtt  >  2:453661/1‑238 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      
TCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTT  >  NC_000913/3951581‑3952069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑