Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,552	A→T	intergenic (+995/‑649)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,552	0	A	T	100.0%	224.2 / NA	65	intergenic (+995/‑649)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (34/31);  total (34/31)

TGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTG  >  NC_000913/3951308‑3951791                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 tGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTacac                                                                                                                                                                                                                                              >  1:321977/1‑251 (MQ=255)                        ttCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCAAACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAAc                                                                                                                                                                                                                        >  1:179799/1‑250 (MQ=255)                         tCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACa                                                                                                                                                                                                                       <  1:377775/250‑1 (MQ=255)                             ggTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAAcctgcct                                                                                                                                                                                                                  >  1:326027/1‑245 (MQ=255)                                gCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGCGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGAACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGc                                                                                                                                                                                                               >  1:72290/1‑251 (MQ=255)                                    gAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTa                                                                                                                                                                                                           <  1:253609/251‑1 (MQ=255)                                      gtgcgacttgctgATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACg                                                                                                                                                                                                         >  2:373581/9‑251 (MQ=255)                                        gcgacttgctgATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGAc                                                                                                                                                                                                       <  1:373581/245‑1 (MQ=255)                                                      gCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGc                                                                                                                                                                                         >  1:344955/1‑251 (MQ=255)                                                         gTGGGTGCACGTTTTGATGAACGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑AAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTGACGACCATCCCGGTGACGCTAt                                                                                                                                                                                      >  2:343191/1‑251 (MQ=255)                                                                  ggTTTTGATGACCGGGTGACCGGCAAACTGAACGCCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGGGGCATGACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑ATAGCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGcc                                                                                                                                                                             <  1:269552/250‑1 (MQ=255)                                                                  cGTTTTGATGACCGGGTGACCGGCAAACTGANCACCTTCGCACCACACGCCCGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCGGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAAGGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGcc                                                                                                                                                                             <  2:227477/251‑1 (MQ=255)                                                                  cGTTTTGAGGACCGGGGGGCCGGCAAACGGACCACCTTCGCACCACACGCCAGGGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGcc                                                                                                                                                                             <  2:55947/251‑1 (MQ=255)                                                                   gTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCg                                                                                                                                                                            >  2:133247/1‑251 (MQ=255)                                                                     ttttatgaCCGGGTGACCTGCAAACTGAATAACTTCGCCCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTGCCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGGCTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCgt                                                                                                                                                                           <  1:44654/246‑1 (MQ=255)                                                                     tttGATGACCGGGTGACCGGCAAACTGAACGCCTTCGCCCCACACGCCAGGTTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCGCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgt                                                                                                                                                                           <  2:147429/250‑1 (MQ=255)                                                                                 gTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTAGCCATAGTGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAaaca                                                                                                                                                              <  1:16133/251‑1 (MQ=255)                                                                                      cGGCAAACTGAACACCTTCGCACCACACGCCAATGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGAGCGCAGCTGCGTGATGAACATGCCTGGCGCTCCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTCAaacacatgt                                                                                                                                                         >  2:135572/1‑246 (MQ=255)                                                                                       ggCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc                                                                                                                                                        >  2:438845/1‑251 (MQ=255)                                                                                               gAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGt                                                                                                                                                 <  2:353319/250‑1 (MQ=255)                                                                                                        cgcaccACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCgg                                                                                                                                       <  1:165522/247‑1 (MQ=255)                                                                                                         gcaccACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCgg                                                                                                                                       >  1:78025/4‑250 (MQ=255)                                                                                                           accACACGCCAGTGTTATCCATATGGATATCCACCCGGCAGAAATGAACAAGCTGCGTCAGTCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCTTAAACCTGCGGAtt                                                                                                                                    <  2:325911/250‑1 (MQ=255)                                                                                                               cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGcgt                                                                                                                                >  1:106543/1‑251 (MQ=255)                                                                                                                   ccAGTTTTCTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTCCCCGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAGCCTGCGGATTGCGTCGTg                                                                                                                            <  2:383347/251‑1 (MQ=255)                                                                                                                    cAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTg                                                                                                                            >  1:288500/1‑250 (MQ=255)                                                                                                                      gtgtTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGAcc                                                                                                                         >  1:237077/1‑251 (MQ=255)                                                                                                                           aTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACcac                                                                                                                       >  2:415599/1‑248 (MQ=255)                                                                                                                                   ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTgggg                                                                                                            <  2:417501/250‑1 (MQ=255)                                                                                                                                          gACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAACACAACTGTCAGATCGTACACCTGCGGCTTGCGTTGTGACC‑CACGATGTGGAGCCGCAccc                                                                                                    >  2:166260/1‑250 (MQ=255)                                                                                                                                           aCCCTGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCAcc                                                                                                     >  1:360699/1‑249 (MQ=255)                                                                                                                                              cGGCAGAAATGCACAAGCTACGTCAGGAACCTGAGGAATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAACCGTTAAATAT‑‑CAATGACTAGCAGCTACACTGCGAGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGAGCCGCTATCTACGCGACGTTGGTGTTAAAACAACTGTCAGATCGTAAACCTGCGTATTGCGTCGTGACCACA‑GATGTGGTGCAGGACCAGAt                                                                                                 >  2:164291/1‑250 (MQ=255)                                                                                                                                              cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GCTGTGGGGCAGCACCAGAtg                                                                                                >  1:214982/1‑251 (MQ=255)                                                                                                                                                 cAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTgg                                                                                             <  2:428243/251‑1 (MQ=255)                                                                                                                                                     aaTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTg                                                                                                                                          >  1:249282/1‑203 (MQ=255)                                                                                                                                                     aaTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTg                                                                                                                                          <  2:249282/203‑1 (MQ=255)                                                                                                                                                       tGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGCTTTAACTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGCTCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGAAGCACAAGATGTGGGCTggg                                                                                       >  2:185370/1‑249 (MQ=255)                                                                                                                                                             aGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcac                                                                                 >  2:94589/1‑251 (MQ=255)                                                                                                                                                                 gCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCATCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCg                                                                             <  1:43910/251‑1 (MQ=255)                                                                                                                                                                  cGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGc                                                                            <  1:287213/251‑1 (MQ=255)                                                                                                                                                                   gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGAt                                                                                                 >  1:198611/1‑229 (MQ=255)                                                                                                                                                                   gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGAt                                                                                                 <  2:198611/229‑1 (MQ=255)                                                                                                                                                                                      aCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCCCACGCGCCCGGAAAAtt                                                        >  2:73134/1‑251 (MQ=255)                                                                                                                                                                                       cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAAttt                                                       >  1:128345/1‑251 (MQ=255)                                                                                                                                                                                                   aaTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAg                                           <  1:151089/251‑1 (MQ=255)                                                                                                                                                                                                   aaTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAg                                           <  1:232671/251‑1 (MQ=255)                                                                                                                                                                                                           gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCggct                                      <  1:98811/248‑3 (MQ=255)                                                                                                                                                                                                           gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTa                                    <  2:377300/250‑1 (MQ=255)                                                                                                                                                                                                           gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAg                                   <  1:227605/251‑1 (MQ=255)                                                                                                                                                                                                           gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGGTACGACCATCCCGGGGACGCTAGCTACTCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCATCACCATATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtcat                                                    <  1:383082/234‑1 (MQ=255)                                                                                                                                                                                                           gTTACCAGCATTACAGCACCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGAGCAGCTGCGTGATGAACATGCCTGGCGTTAAGACCATCCCGGTGACGCTATATACGCGACGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCCGATGTGGGCCGCGCAGCACATCGCACACACTCGCCCGGCAAACTtcatagataggaaccgcgtcg                                   >  2:383082/1‑234 (MQ=255)                                                                                                                                                                                                             tcccAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGCTCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCggcctcggc                                 >  2:9689/3‑244 (MQ=255)                                                                                                                                                                                                               ccAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTTCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggcac                               <  1:398817/251‑4 (MQ=255)                                                                                                                                                                                                                       aCAGCAGCCGTTAAATAT‑‑CACTGACGGGCAGCTACACTGCGCGCCGCTCCGTGATGAACATGCCAGACGATACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGATAAAACAACTGTCAGATCGTAAACCTGCGGTTTGCGTCGTGACCACA‑GATGTGGCCCAGCCCCAGAAGTGGACTGGGCAGCACATCGCCCACACTCGCCAGGAAAATTTCATCACCTCCAGCGCCTTAGGCACCCTgggct                       >  2:380851/1‑249 (MQ=255)                                                                                                                                                                                                                           cagcCGTTAAATAT‑‑CAATGACTGGCCGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCAAA‑GATGTGGGGCAGCACCAGCTGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGATTTgg                   >  2:330203/1‑251 (MQ=255)                                                                                                                                                                                                                           cagcCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTgg                   <  1:264458/251‑1 (MQ=255)                                                                                                                                                                                                                                gttaaataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTAc              >  1:148641/8‑251 (MQ=255)                                                                                                                                                                                                                                  taaataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTAcc             <  1:222899/245‑1 (MQ=255)                                                                                                                                                                                                                                    aataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggc          >  1:403027/4‑251 (MQ=255)                                                                                                                                                                                                                                    aataT‑‑CAATGACTGGCAGCTACACGGCGCGCAGCTTCGGGATGAACATGCCGGGCGGTACGACCATCCCGGTGACGGTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGGGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGGCCACACGCGCCCGGAAAATGTCAGCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggc          <  2:408826/248‑1 (MQ=255)                                                                                                                                                                                                                                      taT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcgg        >  2:442034/2‑251 (MQ=255)                                                                                                                                                                                                                                       aT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCNGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcggc       <  2:237077/251‑1 (MQ=255)                                                                                                                                                                                                                                           cAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcggcgg     >  2:344985/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCCGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGtt   >  2:444950/1‑251 (MQ=255)                                                                                                                                                                                                                                               gACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTg  <  1:383652/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 TGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTG  >  NC_000913/3951308‑3951791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑